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JOURNAL OF EXPERIMENT

Title : SALIVA
Day/Date : Monday, November 20th 20156
Name : Khairul Ashar Chairan
ID : 1413442009
Class/Group : ICP A/ II (Two)
Partnerts : 1. Andi Tenri Tayu
2. Jumriana Jufri
3. Yuli Ratnadillah
Assistant : Marwah Karim

A. OBJECTIVE OF EXPERIMENT
At the end of this experiment in the hope students can:
a) Knowing the mucin test
b) Knowing the saliva test compounds inorganic compounds
c) Knowing thiocyanate test
d) Knowing test the effect of temperature on the activity of ptyalin
e) Knowing test ptyalin
f) Knowing the pH determination test is suitable for employment saliva
g) Knowing the effects of compounds that inhibit / destroying the activity of
bacteria in salivary amylase.
B. APPARATUS AND CHEMICALS
1. Apparatus
a. Measuring cup 10 ml and 20 ml 2 pieces
b. Beaker 50 ml, 250 ml and 600 ml 1 piece
c. Test tube 10 pieces
d. Test tube rack 1 piece
e. 100oC thermometer 1 piece
f. Funnel usual 1 piece
g. Pipette 20 pieces
h. Tripod and gauze asbestos 1 piece
i. Spiritus 1 piece
j. Stopwatch 1 piece
k. Stir bar 1 piece

2. Chemicals
a. Saliva
b. Distilled water
c. BaCl solution of 25%
d. Solution of NH 4 -Oksalat 4%
e. CH3COOH solution 2 N and 0.1N
f. Starch solution 1%
g. reagent millon
h. reagent benedict
i. reagent molisch
j. Solution I2 0.01 M
k. 0.1 M NaCl Solution
l. Filter paper
m. toluene
n. Solution of concentrated HCl
o. chloroform
p. HgCl solution of 1%
q. Phenol 2%
r. AgNO3 solution
s. HNO3 aqueous solution
t. Phenol solution (pH 5, 7, 9)
u. NaF and 0.1 M FeCl
v. Tissue and lighters

C. WORK PROCEDURES
1. Mucin Test
Millon Benedict Mollish
Reagent Reagent Reagent

5 mL saliva 2 drops of Using water as a control by


adding 2 drops of 0.1M acetic Filter the precipitate
acetic acid
formed
0.1M acid 2 drops
Testing

2. Thiocyanate test
If red still means there thiocyanate, if
the red color is missing then no
thiocyanate

5 mL saliva 5 drops of FeCl 1 drop of Using water as a control by 5 drops of HgCl


0,1M concentrated adding 5 drops of FeCl 0.1M and 1%.
HCl 1 drop of concentrated HCl

3. Saliva Test Composer Inorganic Compounds

1 2 3 4

15 mL saliva
Drops with 2 N acetic acid,
forming a precipitate Enter into 4 different reaction
Heated to boiling filtered The filtrate tubes
was inserted
3 mL

Checking ion Cl

1 1

Acidified with Added a few


dilute HNO3 drops of 0.5 M
AgNO3

Checking PO43-
2 2

Acidified with Added 1 mole of reagent


dilute HNO3 ammonium molybdate be heated

Checking ion SO42-

3 3

Acidified with HNO3 (e) Added 1 mL CaCl2 2%

Checking ion Ca2+

Added 1 mL of NH4 - Oxalate 4%

In each experiment carried out observations of the results are instantaneous, and
also after one hour and then take a good look possibility precipitate formed little
that it is difficult to observe.

4. Effect of Temperature Tests Against Activities Ptiatin


1 2 3 4
2 1 2 3

Each tube was added Tube 1 be


cooled tube 2 at room
temperature To the tube was added
5 mL of 1% starch. saliva 1: 9
tube 3 heated to a
temperature of 38oC

Every 5 minutes to take samples and checked tube 4 added salivary that has
in with 2 drops of 0.01 M I2, and record the been heated for 5 minutes
speed of the starch solution in each tube

5. Test Estimation Ptialin


2 mL NaCl 0,1M

10 mL starch 1% Into a test tube inserted 8 3 moles of water and 3 drops of a


solution of 0.01 M I2 1 mL of saliva 1: 9 into a
tube containing starch
Heated at 38oC

Record the time at the time of adding any starch


mixture of saliva in a solution of I2
indeterminate cause the color
If the time it takes less than four minutes to do
the experiment under the same way but saliva
should be diluted 2 times

Intervals of 30 seconds with a pipette take the starch mixture


and insert 2 drops into the tube 8

6. Determination of pH tests from which to work saliva


Heated at 38oC.

5,2
8 7,4 6,8 6

pH 8 pH 7,4 pH 6,8 pH 6 pH 5,2

8 7,4 6,8 6 5,2

10 mL larutan buffer 5 mL pati 1% 2 mL NaCl 2 mL saliva


0,1M 1:9

8 7,4 6,8 6 5,2 8 7,4

Adding I2 and specify in which a tube is missing The addition of acetic acid
the color point is reached so that the mixture becomes
acidic atmosphere
7. Effect compounds that inhibit / destroying the activity of bacteria in salivary
amylase
8 mL
water 5 drops 5 drops
5 drops
toluene chlorofor HgCl2 1%
1 mL liquid salive into 7 reaction tube
m

1 2 3 4 5 6 7 1 2 3

2 mL saliva.

added starch solution 1% 5 mL 5 drops


5 drops 0,5 mg NaF fenol 2%
into each tube Let stand for 10 minutes while stirring occasionally water

1 2 3 4 5 6 7 1 2 3 4 5 6 7 6 5 4

in water 38oC

6 7 each tube
1 2 3 4 5
halved I2 Benedict
solution Solution

Observe and record observations of each


tube

Makassar, November 16th 2016


Asisstant Apperantice

Marwah Karim Khairul Ashar Chairan