DISTRIBUSI KURKUMIN DAN KURKUMINOID PADA TEMU HITAM

(Curcuma aeruginosa Roxb) BERDASARKAN PERBEDAAN BAGIAN

RIMPANG DAN UMUR TANAMAN

Irma Aviani Meilinda Handayani

12/331450/PA/14704

INTISARI

Telah dilakukan isolasi kurkumin dan kurkuminoid dari empu dan anakan
rimpang temu hitam (Curcuma aeruginosa Roxb) untuk mengetahui distribusi
senyawa kurkumin dan kurkuminoid serta mempelajari pengaruh umur tanaman
terhadap kandungan kurkumin dan kurkuminoid.

Metode yang dilakukan dimulai dengan preparasi sampel. Isolasi

kurkumin dan kurkuminoid dilakukan dengan ekstraksi maserasi 24 jam
menggunakan pelarut etanol. Selanjutnya dilakukan optimasi eluen. Eluen yang
diperoleh berupa kloroform:metanol (97:3). Eluen tersebut digunakan sebagai fasa
gerak dalam metode kromatografi lapis tipis preparatif. Hasil yang diperoleh
ditentukan kadar senyawa kurkumin dan kurkuminoid menggunakan TLC
Scanner serta dibuktikan kandungan senyawanya dengan LC-MS.

Hasil yang diperoleh dari isolasi rimpang C. aeruginosa Roxb berupa

kurkumin, demetoksikurkumin dan bisdemetoksikurkumin. Kadar yang diperoleh
bisdemetoksikurkumin>demetoksikurkumin>kurkumin di umur tanaman 3 bulan,
sedangkan kurkumin>demetoksikurkumin>bisdemetoksikurkumin di umur
tanaman 10 bulan. Distribusi ketiga senyawa tersebut lebih besar berada pada
bagian empu dibanding anakan rimpang. Komponen utama pada rimpang
C.aeruginosa Roxb di umur 3 bulan berupa bisdemetoksikurkumin, sedangkan
pada umur 10 bulan berupa kurkumin.

Kata kunci: C. aeruginosa Roxb, isolasi, kromatografi lapis tipis preparatif,

kurkumin dan kurkuminoid, TLC Scanner
 THE DISTRIBUTION OF CURCUMIN AND CURCUMINOID ON
Curcumae aeriginosa Roxb BASED ON THE DIFFERENCE OF RHIZOME
AND AGE OF PLANTS

Irma Aviani Meilinda Handayani

12/331450/PA/14704

ABSTRACT

Isolation of curcumin and curcuminoid from parent and saplings of

Curcuma aeruginosa Roxb rhizome has been conducted to understand the
distribution of curcumin and curcuminoid compound and to study the leverage of
the age of plant on curcumin and curcuminoid contents.

The preparation of sample was the first method to performed. The

isolation of curcumin and curcumionid was carried out with the maseration
extraction for 24 hours using etanol. The next step was to optimized the eluent
and obtained the result of chloroform:methanol (97:3). The obtained results were
used as mobile phase on preparative thin-layer cromatography then determined
the measure and contents of curcumin and curcuminoid compound using TLC
Scanner and LC-MS, respectively.

The results of the isolation of curcumin and curcuminoid on Curcuma

aeruginosa Roxb rhizome are curcumin, demetoxycurcumin and
bisdemetoxycurcumin. The levels obtained are
bisdemetoxycurcumin>demetoxycurcumin>curcumin at the age of 3 months,
curcumin>demetoxycurcumin>bisdemetoxycurcumin at the age of 10 months.
The distribution of the third compounds were higher on the parent than the
saplings. The main components on Curcuma aeruginosa Roxb rhizome at the age
of 3 months are bisdemetoxycurcumin, while at the age of 10 months are
curcumin.

Keywords :Curcuma aeruginosa Roxb, isolation, preparative thin-layer

cromatography, curcumin and curcuminoid, TLC Scanner