HEMATOLOGY

n which organ or tissue do T-lymphocytes mature?

Please select the single best answer

bone marrow

thymus

lymph nodes

spleen

The image on the right contains the fluorescence data from CD45 from a flow cytometry
analysis. Note that virtually all cells in the gated population have CD45 surface antigens,
indicating they are which type of cells?

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Leukocytes

Erythrocytes

Platelets

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The mediator cells that bind MOST to IgE antibodies are:

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Basophils

Eosinophils

Polymorphonuclear neutrophils

Macrophages

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Allergen-specific IgE, synthesized in response to allergens, becomes fixed to receptors on

cellular membranes, especially those of basophils. If these receptor-bound IgE molecules
are aggregated on re-exposure to specific allergen, both mast cells and basophils produce
mediators that result in the allergic response. IgE-antigen interaction at the cell surface
causes degranulation of cells and release of substances including: histamine, SRS-A,
platelet activator, a kallikrein, and an eosinophil chemotactic factor. Basophils are
the principal cells that bind IgE antibody while their number of receptor sites is
proportional to serum IgE levels. Eosinophils are drawn to the site by the
basophil chemotaxis mechanism, but are not the main cell which binds the
IgE

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What should be the first step performed to resolve a case where all forward and reverse ABO
typing results are negative on a patient?

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Wash the patient cells and retest.

Get a new sample and retest.
Test the patient cells with Anti-H Lectin.
Incubate all testing tubes at 22c

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If all results for a patient's forward and reverse type are negative, it is suggested that the
reactions incubate at room temperature for at least 15 minutes to help strengthen any
reactions to become observable.

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This drawing depicts which alpha chain genotype?

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Alpha thalassemia major (Hydrops fetalis)

Alpha thalassemia intermedia (Hemoglobin H Disease)
Normal Adult
Silent Carrier

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The genotype pictured, --/-, is known as Alpha thalassemia intermedia or Hemoglobin H
disease.

When three gene loci of alpha chains are deleted (--/-) or inactive, only 70-90% of
Hemoglobin A is made.

The excess beta chains that remain unpaired form the tetramers of Hemoglobin H.

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In skin tests for allergy, a wheal and flare development is indicative of:

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immediate hypersensitivity

delayed hypersensitivity

anergy

Arthus reaction

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In skin tests for allergies, a skin eruption can follow an injection of an antigen. It is
characterized by redness and swelling; this is caused by a release of histamine. This type of
reaction typically occurs in stages. First, the appearance of an erythematous area at the site
of injury. Next, the development of a flare surrounding the site. Third, a wheal forms at the
site when fluid leaks under the skin.

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In skin tests for allergy, a wheal and flare development is indicative of:

The correct answer is highlighted below

immediate hypersensitivity

delayed hypersensitivity

anergy

Arthus reaction

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In skin tests for allergies, a skin eruption can follow an injection of an antigen. It is
characterized by redness and swelling; this is caused by a release of histamine. This type of
reaction typically occurs in stages. First, the appearance of an erythematous area at the site
of injury. Next, the development of a flare surrounding the site. Third, a wheal forms at the
site when fluid leaks under the skin.