a r t i c l e i n f o a b s t r a c t
Article history: Objectives: Literature data show that excess and primary deciency in particular nutrients, vitamins and minerals
Received 20 November 2016 may lead to pre-eclampsia, gestational diabetes, hypertension and neural tube defects in the foetus. The aim of
Accepted 1 February 2017 the study was to determine differences in average daily consumption of selected nutrients during pregnancy
Available online xxxx in women who did not supplement their diet and to evaluate the inuence of dietary habits on the occurrence
of pre-term delivery and hypertension in pregnant women.
Keywords:
Sample group and methods: Information on the course of pregnancy and the newborn's health status at birth was
Caesarean section
Deciency
derived from the Charter of Pregnancy and documents recorded by the hospital. Women's eating habits and di-
Hypertension etary composition were analyzed on the basis of a dietary questionnaire. The sample group was divided into four
Minerals groups: women who delivered neonates appropriate for gestational age (AGA), women with gestosis who deliv-
Premature delivery ered AGA neonates by means of caesarean sections, women who delivered pre-term neonates (PTB) and women
with gestosis who delivered PTB by means of caesarean sections.
Results: In the case of women with vaginal delivery at term the average intake of iodine was always higher than in
other groups. Analysis of average daily intake of folates revealed a higher intake in the group of women who gave
birth to full-term neonates with proper neonatal weight in comparison with the groups of women with pre-term
delivery. P 0.05.
Conclusions: Statistically signicant differences in average daily intake of folates, iodine, retinol, magnesium and
iron were observed between the group of women with vaginal delivery at term and the groups of women with
diagnosed hypertension who delivered preterm. Correlation was demonstrated between average daily intake of
iodine and vitamin D and the occurrence of arterial hypertension. Supplementation of the diet of women in the
preconception and prenatal period with minerals and vitamins should be considered.
2017 Elsevier Inc. All rights reserved.
1. Introduction WHO, 2010). The remaining energy demand, i.e. 4560%, should be pro-
vided by carbohydrates (Jarosz, 2012; WHO, 2007b).
The diet of a pregnant woman, which conditions proper development Studies have shown that supplying an insufcient amount of protein
of the foetus, should be the source of wholesome protein, fats and to the body during pregnancy has an adverse effect on foetal develop-
carbohydrates and take into account recommendations on the right pro- ment and birth body weight of neonates, and low birth weight is associ-
portions and caloric value which vary depending on the trimester of preg- ated with the risk of hypertension, diabetes, obesity or hyperlipidemia
nancy. According to recommendations of the Food and Nutrition Board, at a later age (Jarosz, 2012; Akbari, Mansourian, & Kelishadi, 2015;
daily intake of protein for pregnant women is about 1 g per kilogram of Ostachowska-Gsior, 2008). An excessive intake of carbohydrates dur-
body weight, which constitutes 1015% of daily energy demand (WHO, ing pregnancy is associated with the risk of hyperglycemia for the moth-
2007a) Consumption of fats should be an average of 2530% of daily en- er and leads to hyperglycemia and hyperinsulinemia in the foetus,
ergy demand. During pregnancy, depending on the trimester, fat content leading to increased protein synthesis in the foetus and deposition of
in the diet should be increased by 1115 g daily on average, an adequate fat and glycogen in tissues, which result in macrosomia and the occur-
content of fatty acids should also be taken into account (Jarosz, 2012; rence of polyhydramnios (Wang, Jiang, Yang, & Zhang, 2015).
An important element of proper nutrition of pregnant women is the
supply of adequate amounts of vitamins and minerals, especially those
Corresponding author at: Faculty of Health Sciences, Department of Nursing and
Emergency Medicine, University of Bielsko-Biala, ul. Willowa 2, 43-300 Bielsko-Biala,
scarcely present in food products.
Poland. In accordance with recommendations concerning deciency of vita-
E-mail address: wwaksmanska@ath.bielsko.pl (W. Waksmaska). min D - daily intake of the vitamin for pregnant women is 2025 g.
http://dx.doi.org/10.1016/j.apnr.2017.02.013
0897-1897/ 2017 Elsevier Inc. All rights reserved.
14 W. Waksmaska et al. / Applied Nursing Research 35 (2017) 1317
Literature data show that vitamin D affects expression of genes regulat- To obtain a homogeneous group of women, the following inclusion
ing angiogenesis. During pregnancy vitamin D is essential to maintain criteria were applied:
the foetus. Vitamin D deciency may lead to the occurrence of pre-
1. Polish nationality (excluding naturalized Polish citizens); single
eclampsia, gestational diabetes, and in the foetus - to the development
pregnancy; pregnancy IIII (consider parity);
of rickets (Gruber, 2015; Misiorowska & Misiorowski, 2014; Robinson,
2. Stable socioeconomic status. Socioeconomic status was determined
Wagner, Hollis, Baatz, & Johnson, 2011; Sadin, Pourghassem Gargari,
based on factors such as income, marital status, education, place of
Fard, & Tabrizi, 2015).
residence. Women eligible for the study were married, had good
Calcitriol - an active form of vitamin D, affects calcium-phosphate
housing conditions, had secondary or higher education level; lived
metabolism of the organism and inhibits secretion of parathormone
in a highly industrialized urban region, both the women and their
(Misiorowska & Misiorowski, 2014).
husbands had a steady job;
Another important vitamin in the diet of pregnant women is vitamin
3. Following a typical diet for the Polish population. The diet of the
B9, also called folic acid. The consumption norm concerning folates dur-
women was determined on the basis of Album of Photographs of
ing pregnancy is 600 g.
Food products and Dishes (Szponar, Wolnicka, & Rychlik, 2010)
Folates participate in the synthesis of red cells and joining of alkyl
(the women accepted into the study were neither vegetarians nor
groups to nucleobases. They are also a cofactor of metabolic changes
followed any other special diets). Their diet was not modied in
of homocysteine (Kapka-Skrzypczak, Niedwiecka, Skrzypczak, &
any way;
Wojtya, 2012).
4. Granting consent to participate in the study.
Deciency of folates leads to an increase in homocysteine concentra-
The following exclusion criteria were applied:
tion, which is a risk factor of development of thrombotic disorders. Dur-
ing the preconception period and early pregnancy, deciency of folates 1. Chronic diseases occurring in the women before pregnancy, such as
may lead to neural tube defects in the foetus, and later in pregnancy, it chronic hypertension, pre-gestational diabetes; infections during
may increase the risk for premature birth, the occurrence of pre- pregnancy (any kind of infection in the perinatal period, such as
eclampsia and hypertension (Kapka-Skrzypczak et al., 2012; Cielik & fever, respiratory infections, urinary infections, etc.); miscarriages
Kociej, 2012; Seremak-Mrozikiewicz, 2013). and/or premature birth resulting in the death of the child or develop-
Another important element of the diet during pregnancy is iodine. mental anomalies in the foetus;
Daily intake of iodine recommended by WHO is 250 g (Hubalewska- 2. AIDS and sexually transmitted diseases;
Dydejczyk, Lewiski, Milewicz, et al., 2011). Iodine is essential for the 3. Adherence to a vegetarian diet, Mediterranean diet, or any other spe-
production of the thyroid hormone - thyroxine, which participates in, cial diet;
among others, proliferation of neural cells of the developing foetus 4. Lack of the mother's consent to take part in the research program or
(Zygmunt & Lewinski, 2015). withdrawal of consent during the study.
Severe iodine deciency during pregnancy may lead to an increased Women who participated in the research program were classied
risk of miscarriage, preterm delivery, impaired foetal lung development, into four groups according to the following criteria:
deafness, damage to the central nervous system and even to irreversible -Group A - Group of women who delivered neonates appropriate for
brain underdevelopment in the foetus (Zygmunt & Lewinski, 2015). gestational age. (AGA), healthy mothers, routine, uneventful pregnancy
The risk of hypertension in pregnant women is increased in the case (neonatal weight 10th90th percentile) (n = 11),
of magnesium deciency. Magnesium is a cofactor of carbohydrate, fat -Group B - Group of women who delivered neonates appropriate for
and protein metabolism. Magnesium takes part in glycolysis, calcium gestational age by means of caesarean sections due to gestational high
homeostasis, vitamin D hydroxylation and synthesis of high-energy blood-pressure (neonatal weight 10th90th percentile) (n = 10),
bonds (ATP, GTP). By creating complex formations with phospholipids, -Group C - Group of women who delivered preterm neonates (PTB),
magnesium is also part of the structure of cell membranes (Iskra, healthy mothers, routine, uneventful pregnancy (n = 7),
Krasiska, & Tykarski, 2013). -Group D - Group of women who delivered preterm neonates (PTB),
Pregnancy complications concerning both the mother and foetus af- by mean of caesarean sections due to gestational high blood-pressure
fect the increase of perinatal mortality, which constitutes an important (n = 8) (Table 1).
public health issue. Women eligible for the study underwent three ultrasound examina-
To date, research has proved that women's awareness of balanced tions (between the 12th and 14th weeks of gestation, between the 20th
nutrition during pregnancy and the inuence of nutrition on the health and 22nd weeks and between the 32nd and 33rd week). All the foetuses
of the mother and her future child is insufcient, both in terms of choice had normal karyotypes and no malformation at birth.
of nutritional ingredients and dietary supplements (Akbari et al., 2015; The mothers did not receive any dietary supplementation during
Kapka-Skrzypczak et al., 2012; Seremak-Mrozikiewicz, 2013; Zygmunt pregnancy. The mothers in PTB did not receive corticosteroids. Those
& Lewinski, 2015; Hamuka, Wawrzyniak, & Pawowska, 2010; Suliga, children whose weight exceeded the 90th percentile (Large for gesta-
2013; Waszkowiak, Szymandera-Buszka, & Szewczyk, 2010). tional age - LGA) were not included in the study.
The information on the course of pregnancy of the women came
from the Charter of Pregnancy, i.e. the document recorded by the gyne-
2. Aim of the study
cologist who took care of the pregnant woman. All the information
The aim of the study was to determine differences in average daily
consumption of selected nutrients during pregnancy in women who
did not supplement their diet, and to evaluate the inuence of dietary Table 1
habits on the occurrence of preterm delivery and hypertension in preg- Analysis of the studied groups, g - gram.
nant women. Studied Size of Mean age of Birth order Average birth
group group participants dened in % weight (g)
concerning the birth and postpartum period, the newborn's health sta- 5. Statistics
tus at birth and the results of their physical examination was taken from
the documents recorded by the hospital. The Kruskal-Wallis ANOVA test was used to determine differences in
The study was approved by the Bioethics Review Board of Bielsko- food intake by the three groups. The Kruskal-Wallis nonparametric
Biala (No: 2016/02/11/4) which is in accordance with the Declaration analysis of variance was used due to high absolute values of the skew-
of Helsinki. ness and kurtosis of a large number of the variables. A detailed post
hoc analysis was also conducted.
4. Procedures The statistical signicance was determined at P b 0.05.
Gestational age was calculated according to the Naegele's rule. The
The women's eating habits and dietary composition were ana- mean age of subjects ranged between 27 and 32 years. In the group of
lyzed based on a monthly dietary questionnaire designed by the women who delivered at term it was second pregnancy for them in
National Food and Nutrition Institute in Warsaw, Poland. The 91%. For women who gave birth before the due date it was the rst preg-
women specied their diet during the last month of their pregnancy. nancy in 43%, and in 57% - the third pregnancy. Women diagnosed with
Portion sizes were veried using The Album of Photographs of Food hypertension who at the same time had a caesarean section performed
Products and Dishes (Szponar et al., 2010). Before the research before the due date it was their rst pregnancy in 100% (Table 1).
began every participant was clearly instructed and offered training
on how to ll in the questionnaire as well as how to record the vol- 6. Results
ume or the mass of foods using standard household measures such
as a spoonful, a glass, etc. The mothers were not given any hints or The highest average daily intake of fatty acids has been recorded in
tips on how to diet. Based on the album, the women lled in the the group of women with full-term delivery by means of caesarean sec-
questionnaire specifying the quantity and quality of the food con- tion (group B). Rather considerable differences concerning average
sumed. The questionnaire allowed the researchers to determine the daily intake of calcium, magnesium, iron and vitamin D have been re-
daily consumption of each particular dietary component (proteins, corded between women who delivered vaginally at term (group A)
carbohydrates, fats, fatty acids, vitamins) as well as the mother's and other studied groups. In the case of women with vaginal delivery
calorie consumption over a one-month period. at term (group A) the average intake of iodine was always higher (and
The questionnaire included a list of products grouped according to statistically signicant) than in other groups. Analysis of average daily
the following 22 food groups: milk and dairy products, eggs, meat, sau- intake of folates revealed a higher intake in the group of women who
sages, offal, sh, seafood, animal and vegetable fats, vegetables, fruit and gave birth to full-term neonates with proper neonatal weight (group
fruit-products, potatoes and potato-based products, seeds, legumes, ce- A) in comparison with the groups of women with preterm delivery
reals and cereal-products, pre-cooked ready meals, salty snacks, nuts (groups C and D). Lower than recommended norms of folates and iodine
and grains, sugar and sweets, soft drinks, alcohol, soup concentrates, consumption were reported in all analyzed groups (Table 2).
sauces and spices. The study participants had to record how frequently The highest average daily cholesterol consumption was reported in
they consumed each product, with the options being: daily, several the group of women with preterm vaginal delivery (group C). When
times a week, once a week, 23 times a month or never. The question- compared to women diagnosed with hypertension and who gave
naire was lled in by each mother in the hospital, three days after child- birth to pre-term neonates by means of caesarean section (group D),
birth. The DIETA FAO program, which includes data on 1067 typical women who gave birth to full-term neonates (group A) consumed
Polish dishes or food products, was used to estimate the quantity of daily more retinol, folates, iodine, magnesium and iron on average.
the aforementioned components. Dietary consumption was validated The differences were statistically signicant. No statistically signicant
via the Food Intake Frequency Questionnaire, a 7-day nutritional sur- differences concerning average daily consumption of nutrients were re-
vey. The method employed was to write down all the products and corded in the group of women who gave full-term birth by caesarean
dishes that were consumed each day, for a period of 7 days. Food por- section due to arterial hypertension (group B) when compared to
tion sizes were given in household measures, and in the case of some women with preterm vaginal delivery (group C).
products, where possible, also in grams. The sizes of portions and food No statistically signicant differences between average values of
products quantities were veried via direct consultation with each daily intake of proteins, fats, carbohydrates and mineral substances
member of the study, using the Album of Photographs of Food Products were found in groups of women who gave preterm birth regardless of
and Dishes (Szponar et al., 2010). the type of delivery (Table 2).
Table 2
Presentation of average daily intake of selected nutrients in analyzed groups.
Studied variable and unit Group A Group B Group C Group D Statistical signicance Daily norm of average consumption for pregnant woman
Average caloric value of daily calorie intake 2262 2320 2150 2005 No 25003000 kcal
Protein (g) 101.8 98.41 93.09 87.36 No 0.98 g/kg of bodyweight
Fats (g) 88.49 99.66 85.55 76.18 No 70115 g
Carbohydrates (g) 286.28 277.46 271.69 260.23 No 175 g
Saturated fatty acids (g) 38.07 40.09 34.45 29.98 No Lowest possible
Monounsaturated fatty acids (g) 33.49 38.55 31.59 29.89 No Not determined
Polyunsaturated fatty acids (g) 10.31 13.96 12.72 10.46 No Not determined
Calcium (mg) 903.02 783.50 793.31 738.54 No 8001100 mg
Vitamin D (g) 5.13 3.46 4.17 2.95 No 2025 g
Folates (g) 317.15 261.02 236.67 219.53 Yes: A and C; A and D 600 g
Iodine g 221.79 128.06 152.27 111.65 Yes: A and B; A and C; 250 g
A and D
Retinol (g) 431.87 474.36 433.09 264.59 Yes: A and D; B and D 530 g
Magnesium (mg) 373.73 327.19 332.95 300.26 Yes: A and D 300335 mg
Iron (mg) 13.03 12.97 12.22 10.41 Yes: A and D 23 mg
Cholesterol (mg) 424.73 480.60 488.44 244.44 Yes: B and D Not determined
16 W. Waksmaska et al. / Applied Nursing Research 35 (2017) 1317
group of women with vaginal delivery at term and the groups of Kopaski, Z., & Maslyak, Z. (2014). Magnesium in human physiology and pathology.
JPHNMR, 1, 46.
women with diagnosed hypertension who delivered preterm. Laskowska-Klita, T., Chechowska, M., Ambroszkiewicz, J., & Gajewska, J. (2012). Folic acid
2. Correlation was demonstrated between average daily intake of io- Its role in the cellular metabolism. Bromat Chem Toksykol, 2, 144151.
dine and vitamin D and the occurrence of arterial hypertension. Misiorowska, J., & Misiorowski, W. (2014). The role of vitamin D in pregnancy. Post Nauk
Med, 12, 865871.
3. The content of iodine and folates in the diet of women with preterm Mitchell, P. J., Cooper, C., Dawson-Hughes, B., Gordon, C. M., & Rizzoli, R. (2015). Life-
delivery was statistically lower compared to women who gave full- course approach to nutrition. Osteoporosis International, 26, 27232742.
term birth. Ostachowska-Gsior, A. (2008). Protein intake in diet of pregnant women with correct
and incorrect pre-pregnancy nutritional status versus the newborns' body mass.
4. Supplementation of the diet of women in the preconception and pre-
Problem Hig Epidemiology, 89(4), 537542.
natal period with minerals and vitamins should be considered. Ramage, S. M., McCargar, L. J., Berglund, C., Harber, V., Bell, R. C., & the APrON Study Team
(2015). Assessment of pre-pregnancy dietary intake with a food frequency question-
naire in Alberta women. Nutrients, 7(8), 61556166.
Acknowledgments
Robinson, C. J., Wagner, C. L., Hollis, B. W., Baatz, J. E., & Johnson, D. D. (2011). Maternal
vitamin D and fetal growth in early-onset severe preeclampsia. American Journal of
Our grateful thanks to the women who participated in the survey. Obstetrics and Gynecology, 204(6), 14 (556e).
Sadin, B., Pourghassem Gargari, B., Fard, P., & Tabrizi, F. (2015). Vitamin D status in pre-
eclamptic and non-preeclamptic pregnant women: A case-control study in the
References North West of Iran. Health Promotion Perspective, 5(3), 183190.
Seremak-Mrozikiewicz, A. (2013). The signicance of folate metabolism in complications
Akbari, Z., Mansourian, M., & Kelishadi, R. (2015). Relationship of the intake of different of pregnant women. Ginekologia Polska, 84, 377384.
food groups by pregnant mothers with the birth weight and gestational age: Need Suliga, E. (2015). Eating habits and nutritional status of pregnant women and the course
for public and individual educational programs. Journal of Education Health and xoutcomes of pregnancy. Student Medicine, 31(1), 6065.
Promotion, 4, 16. Suliga, E. (2013). Economic and social factors and the quality of nutrition of pregnant
Bobiski, R., & Mikulska, M. (2012). Placental transport and metabolism of long polyun- women. Student Medicine, 29(2), 160166.
saturated fatty acids. Advances in Cell Biology, 39(4), 697708. Szponar, L., Wolnicka, K., & Rychlik, E. (2010). Album of photographs of food products and
Cielik, E., & Kociej, A. (2012). Folic acid Occurrence and signicance. Problem Hig dishes. Poland, Warsaw: National Food and Nutrition Institute.
Epidemiology, 93(1), 17. Wang, H., Jiang, H., Yang, L., & Zhang, M. (2015). Impacts of dietary fat changes on preg-
Gietka-Czernel, M., Dbska, M., Kretowicz, P., et al. (2010). Iodine status of pregnant nant women with gestational diabetes mellitus: A randomized controlled study. Asia
women from central Poland ten years after introduction of iodine prophylaxis pro- Pacic Journal of Clinical Nutrition, 24(1), 5864.
gramme. Polish Journal of Endocrinology, 61(6), 646651. Waszkowiak, K., Szymandera-Buszka, K., & Szewczyk, M. (2010). The contribution of dairy
Gruber, B. (2015). The phenomenon of vitamin D. Postpy Higieny i Medycyny products to iodine intake in pregnant women's diet. Problem Hig Epidemiology, 91(4),
Dowiadczalnej, 169, 127139. 560563.
Hamuka, J., Wawrzyniak, A., & Pawowska, R. (2010). Assessment of vitamins and min- World Health Organisation. FAO (2010). Report of an expert consultation: Fats and fatty
erals intake with supplements in pregnant women. Roczn PZH, 61(3), 269275. acids in human nutrition. Geneva, Switzerland: World Health Organisation.
Hubalewska-Dydejczyk, A., Lewiski, A., Milewicz, A., et al. (2011). Management of thy- World Health Organisation (2007a). Joint WHO/FAO/UNU expert consultation: Protein
roid diseases during pregnancy. Polish Journal of Endocrinology, 62(4), 362381. and amino acid requirements in human nutrition. World Health Organization
Iskra, M., Krasiska, B., & Tykarski, A. (2013). Magnesium Physiological role, clinical im- Technical Report Series, 935, 1265.
portance of deciency in hypertension and related diseases, and possibility of supple- World Health Organisation (2007b). Joint FAO/WHO. Scientic update on carbohydrates
mentation in the human body. Arterial Hypertens, 17(6), 447459. in human nutrition. European Journal of Clinical Nutrition, 61, 132137.
Jarosz, M. (2012). The standard of nutrition for the Polish population Amendment. Poland, Zygmunt, A., & Lewinski, A. (2015). Iodine prophylaxis in pregnant women in Poland
Warsaw: National Food and Nutrition Institute. Where we are? Thyroid Research, 8, 817.
Kapka-Skrzypczak, L., Niedwiecka, J., Skrzypczak, M., & Wojtya, A. (2012). Folic acid
Effects of deciency and justication for supplementation. MONZ, 18(1), 6569.