Business Case Presentation

Production of Powerful Immune Stimulant beta-Glucan

and omega-3-Oils from Marine Diatoms

Innovative Algae Production Concept based on our own Knowledge and Experience from
Brazil, France, The Netherlands, Germany and Norway over several decades
Presented by Morten Laake & Pl Myhre & Lisanne van Dam
Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Background
AlgaVida has acquired exclusive license from SINTEF TTO
30
SINTEF Fisheries & Immunomodulatory glucans from microalgae: Survival of cod Gadus morhua
Aquaculture patented the use 25
larvae fed glucans isolated from diatoms and yeast (commercial)
*
of b-glucan from diatoms
after discovering that the 20

% survival
specific b-glucan
chrysolaminaran extracted 15
from the diatom Chaetoceros
muelleri (CM) show a 40% 10
stronger effect on the
immune system- dependent 5
fish larval survival compared
to other beta-glucans in the 0
Glucan TW Glucan CM Commercial
Macrogard glucan Control
feed market.
(Macrogard)
Skjermo et al, 2006

In 2005 the application of diatoms was Patented in 36 countries WO 2004105775

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
The Algae & the Product Segments

Target markets of biorefinery end products

Aqua feed

Poultry feed

Pig feed

Horse feed

Dog feed
Name Chaetoceros muelleri

Class Diatom Supplements

Size Length: 3-20 m

Lipid 30 % of dry matter Cosmetic

EPA 12-17 % of total lipid

DHA 2 % of total lipid Pharmacy

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Feasibility analysis

Case Study Brazil: Theoretical Max Productivity of

Chaetoceros muelleri in Raceway with the Sun as Light source
Simulation conditions based upon open raceways:
- Nitrogen source: NO3-
30 000
- No carbon limitation 27 047
26 412
25 594 25 816 25 910
- No effect of the variation of the culture temperature 25 000
24 836
23 730
23 063
22 430
- 300 days of production a year

Production mensuelle (kg de MS)

21 855 22 030
20 832
- Harvesting: 1/3 cultivation volume pr. day 20 000

- Number of raceways: 300

15 000
- Surface by raceway: 375 m
- Total production surface: 112 500 m
10 000

Total algal biomass production:

5 000
289 553 kg DM/year
0
Equivalent to 25 tons/ha/yr. on 300 days

Feasibility analysis for AlgaVida executed by AlgoSource, France

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Feasibility analysis

Revenues Prospects of a Full-size

Commercial Production Plant in Brazil
Product k$/ton ton/yr. k$/yr.
Silica powder 3 100 300
Microalgae oil 140 45 6 300
Protein powder 3 75 225
B-glucans powder 100 28 2 800
REVENUES 9 600

Feasibility analysis for AlgaVida executed by AlgoSource, France

Conclusions:
The plant will be very profitable at 10% of World b-Glucan market scale
Exploiting waste or cheap resources is expected to improve profitability
Increasing demand of immuno-stimulants for pharmaceutics add to this

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Norwegian medium size production depend
on closed and artifical light PBR

LGem B.V.
GemTube 250 L
PBR Pilot Unit

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Conclusions of the PBR technology tests
make GemTubes likely choice for Norway
PHOTO-BIORECTOR TECHNOLOGY- AlgaVida AS and LGem B.V.
Although Chaetoceros mlleri is senstive to shear stress it thrives with the
GemTube design, which is radically different from conventional tube PBRs:
Mixed gas and liquid phase avoid horizontal gradients and oversaturation of gases
Air-mediated liquid transport radically reduces energy for pumping and shear stress
Mass transfer for nutrients uptake is very effective due to rolling waves in the tubes
Cell density by microscopy at comparable incident light level matches the
hanging bag cultures when stationary phase is reached after 8 days
Silicate depletion due to low solubility is a problem only during batch growth
and should not happen during semi-continous growth
Observed productivity allow closed mass production in commercial scale
already at 125 mol/m2/s daylight; we now will test even higher levels

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
BUSINESS OPPORTUNITY - AlgaVida AS:
Why not join a winning team in time?
Production of b-1.3,1.6-glucans with PUFAs as secondary
product is profitable in the medium scale of 60 T DW/yr
With Norwegian labour costs, hydro-electricity prices, artificial
fertilizers, silicate and salt water - and climatic conditions; and
With artifical-light year-around production in a dedicated factory
setting a high level of product quality control can be reached; but
It becomes a WINNER for both climate and economy with biobased
energy for heat and electricity, and nutrients re-generated on site
We have decided to move into Phase 3 in 2016: Building
our 1000 m2 Diatom Factory just here by the Oslo Fjord!

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
The AlgaeVida Expertice & Startup-Team

Lisanne van Dam, CTO

M.Sc. Biotechnology, WUR Algae Parc

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016
Small Investors and R&D Partners are wellcome to join our Adventure

TODAY WE INVITE YOU ALL TO BUY 10% OF THE COMPANY

Read our Handout and Contact us: Morten Laake & Pl Myhre

ALGAVIDA AS E-MAIL morten.laake@sigtuninnovation.no

Microalgae Production PHONE +47 913 56 766 / 480 99 110

Morten Laake: AlgaVida Presentation at the SIG Microalga Work Shop - Oslo Science Park - February 4th, 2016

WEB to be launched: www.algavida.com