PART 1: Diffusion
Introduction:
The movement of particles into and out of a cell through the plasma
movements, and cellular energy. One of these forces is known as diffusion. Diffusion
is defined as the random movement of molecules, atoms, or ions from an area of relative
that is applicable to all movement of particles through a plasma membrane; this process is
extremely important for the function of a cell. Only certain particles can diffuse across a
membrane without any help from proteins. In this lab, we will focus on the factors that
inhibit or promote diffusion by using dialysis bags, mixed glucose and starch solution,
and Iodine solution to determine which molecules will move through the permeable
membrane, which in this case is the dialysis bag. The hypothesis for this lab is that the
glucose will move through the dialysis tubing, but the starch will not because of its large,
complex 3D shape.
Materials:
The first step in this lab was to take a strip of dialysis tubing and cut a piece of
about six inches off. The tubing was dipped in water to make it easier to open. Once the
tubing was opened, one end was tied off with string and 30 ml of soluble starch and
glucose solution was poured into the tubing, and then the other end of the tubing was tied
with some more string. Before pouring the glucose solution into the dialysis tube, a
glucose test tape strip was used to confirm that there was glucose in the solution by
putting the strip into the solution and waiting a few minutes. The strip turned a dark
A cup was filled with water, and then 20 ml of Iodine solution was added to the
water, which turned the water a gold color. The purpose of adding Iodine to the water is
that Iodine detects starch in a solution, and causes a chemical reaction that turns the
solution blue. By using the Iodine, observers can detect if the starch moves through the
dialysis membrane. After adding the Iodine to the water, the dialysis tubing was put into
Results:
After letting the dialysis tubing sit in the water for a few minutes, the first
observable change was that the solution inside the tubing had turned a color of blue. This
indicated that the Iodine had moved through the membrane into the extracellular
fluid, which meant that the tubing was permeable to Iodine. The water remained a gold
color, which indicates that the starch present inside the solution did not move out of the
tubing. This means that the pores of the membrane were not big enough to allow the
diffusion of starch. We then used another glucose test tape strip to determine if any
glucose had moved into the water. The glucose strip turned brown for a second time,
indicating that the membrane pores were big enough to allow transport of glucose
molecules, and that the glucose did diffuse into the water.
Based on our results, I would rank the following items in order from smallest to
largest as follows: water, Iodine, glucose, membrane pores, starch. If the experiment was
repeated but sucrose was used instead of glucose, the results would most likely stay the
Compared to starch, which is a long chain of glucose molecules bound together, sucrose
is much smaller than starch and relatively similar to glucose, so it should be able to move
through the membrane. Possible sources of error include the string tied to the tubing
being too loose and allowing molecules to flow through the open tube, instead of through
the membrane; also, the tubing might not have been submerged in the water long enough
for the diffusion to reach equilibrium, or the point where no net movement of molecules
PART 2: Osmosis
Introduction:
permeable membrane. Water is an essential molecule for cellular function, and can exist
in different concentrations in and out of a cell. Human cells function best in a state where
the water to solute concentrations are equal inside and outside of the cell, also known as
isotonic. Plant cells are best suited for an environment that is known as hypertonic, where
the solute concentration is higher inside of the cell than outside of the cell, causing water
to move inside the cell. In other words, when plant cells have more solutes inside of them
than outside, water will rush into the cell. If a cell has less solute than outside of the cell,
water from inside the cell will rush out of it, and the cell is referred to as hypotonic.
In the purpose of this lab, dialysis tubing is filled with different molarities of
sucrose solution and put inside a cup full of distilled water. The movement of water into
and out of the cell, or osmosis, is determined by the concentration of solutions inside the
dialysis bag. The masses of each solution will be recorded before and after being
submersed in water, and the percent changes will be calculated to represent a correlation
in the solute concentration and the movement of water into and out of the tubing.
Materials:
The first step in this lab was to take a strip of dialysis tubing, open it, and pour 10
ml of the designated solution into the tube. For the sake of time, each group did a
different molarity solution and the results were shared amongst each other. After pouring
the solution into the tube and tying the ends while leaving some space for the expansion
of the contents in the bag, the tubes were dried and weighed, and the masses were
recorded. Then the tubes were put into a flask of distilled water and left to sit for
approximately thirty minutes. After thirty minutes, the tubes were removed, dried, and
The following shows the initial masses, final masses, and percent changes of all
Graphing this data creates a visual correlation in the percent change, or dependent
variable, relative to the molarity, or independent variable, of the sucrose solution. A trend
line, the trend line equation, and the R2 value have been included.
Solution Molarity
As the graph shows, as the molarity of the sucrose solution increased, the percent
change in the mass also increased. This is because the water had a tendency to
diffuse across the dialysis membrane into the glucose solution, because the contents
of the bags became steadily more hypertonic, causing more water to move into the
bags. When the dialysis bags were tied, a space was left so that water can move into
and out of the tube without it bursting. Using the trend line and the corresponding
equation, as well as the percent change of the unknown solution, we can calculate
y = 0.251x + 0.003
x = .3767 M
The following table describes the changes that would happen if all of the dialysis
Introduction:
Water potential is known as the prediction of which direction water will
move in a living cell. Water potential is measured by finding the sum of the solute
potential and the pressure potential of an object. Water will move into and out of a
cell depending on the concentration of solutes inside and outside of the cells. If there
is more solute outside of a cell, water will move out of the cell into the solvent. If
there is more solute inside the cell, water will move into the cell. Water will always
move from an area of higher water potential to an area of lower water potential.
The purpose of this lab is test water potential in potato cells by submerging
them in various solutions, including distilled water and five other sucrose solutions
with different molarities. To save time, the different solutions were split among the
groups of the class and the results were shared. The percent change in the mass of
the potatoes after being submerged in the solution can be used to describe a trend
Materials:
The first step in this lab was to pour 50 ml of a sucrose solution with a 0.6
molarity into a beaker. Next, a cork borer was used to cut two potato cylinders out
of the available potato. The cylinders were approximately 3 cm long. The skin on the
potato cylinders was cut off to give accurate results. The mass of the two cylinders
was recorded, and then they were placed in the beaker with the sucrose solution
and left overnight. After approximately 24 hours, the cylinders were removed from
the beaker, dried and weighed, and their new masses were recorded.
Results:
The following table lists the various sucrose solutions, the initial masses, final
The following graph shows the trend line of the percent changes (dependent
variable) in relation to the solution molarity (independent variable). The trend line
Percent Change
y = -0.5486x + 0.1851
R = 0.9749y = -0.5486x + 0.1851
Solution Molarity R = 0.9749
As the graph shows, as the molarity of the sucrose solution increased, the
amount of water in the potato cells steadily increased. This is due to the fact that as
the solutions molarity steadily increased, the potato cells became more and more
hypotonic relative to the solute concentration outside of the cells, and so the water
1. To determine the molarity of the sucrose solution, which is isotonic, the trend line
y = -.548x + 0.185
0 = -.548x + 0.185
x = .338 M
2. To calculate the osmotic potential of the isotonic sucrose solution from the last
potential of the potato cells would become more negative because the water would be
evaporating from the potato. When water leaves a cell, the osmotic potential becomes
more negative.
4. Based on the results of this experiment, spraying water on fruits and vegetables would
help maintain their freshness. Water will dehydrate from the plant as time progresses, and
so the plant cells with start to become flaccid. If water is sprayed on the plants, the cells
should absorb the water and become turgid again. If you think of this in terms of the
graph and the trend line, when the potato was put in distilled water, the greatest
5. Adding too much fertilizer with mineral salts in it can cause plants to wilt. The salts in
the fertilizer create a hypertonic environment outside of the cell, so the water in the cells
will diffuse out. The loss of water in the plant cells will decrease the turgor pressure that
the plant cell walls give off, which will consequently cause the plant to wilt.
6. The value of the solute potential of the unknown solution in the potato experiment was
.338 molarity. The value of the solute potential of the unknown solution in the potato
experiment was .378 molarity. The values are relatively close to each other, which shows