MSP WITHOUT INHIBITOR RAW DATA KINETICS

0m 0.1m 0.2m 0.3m 0.4m 0.5m 0.6m

M M M M M M M
[S] V 1/[S] 1/V
bla 0 0 0 0 0 0 0
0.1
nk 0.0379 10 26.340
0 0 64 0.07 0.04 43
0.07 0.06 0.06 0.05
0.3 0.0460 3.3333
9 21.719
2 3 2 3
30 0 42 0.31 330.38 30.51 0.43 0.33 0.68
0.5 0.0484
7 2 5 20.633
4 1 6 8
60 0 65 0.47 0.68 33
0.75 0.76 0.57 0.95
1 9 7 7 3 5
90 0 0.60 0.90 1.01 1.16 0.86 1.14
9 6 6 6 7 9
120 0 0.71 1.09 1.35 1.35 1.05 1.36
4 8 2 2 9 6
150 0 0.80 1.23 1.49 1.51 1.30 1.52
8 4 5 5 5 3
180 0 0.88 1.34 1.57 1.57 1.41 1.59
9 5 5 3 4 6
210 0 0.95 1.43 1.62 1.63 1.53 1.64
3 9 5 2 8 2
240 0 1.01 1.49 1.62 1.65 1.58 1.65
2 5 7 7 5 9
270 0 1.06 1.53 1.67 1.67 1.62 1.67
2 2 4 4 9 6
300 0 1.10 1.56 1.68 1.68 1.64 1.68
2 6 2 3 5 4
330 0 1.13 1.58 1.69 1.68 1.66 1.69
6 7 1 3 1 3
360 0 1.16 1.61 1.7 1.68 1.67 1.69 CORRECTED DATA
9 3 3
0m 0.1m 0.2m 0.3m 0.4m 0.5m 0.6m
390 0 1.19 1.61 1.7 1.68 1.67 1.69
M M M M M M M
5 8 3 8 3
0 0 0 0 0 0 0 0
420 0 1.21 1.62 1.7 1.68 1.68 1.69
30 0 0.247 0.336 0.442 0.368 0.274 0.635
8 6 3 7 3
60 0 0.401 0.64 0.685 0.704 0.511 0.902
450 0 1.21 1.63 1.7 1.68 1.68 1.69
90 0 0.539 0.857 0.944 1.103 0.805 1.096
8 4 3 7 3
12 0 0.644 1.049 1.28 1.289 0.997 1.313
0
15 0 0.738 1.185 1.423 1.452 1.243 1.47
0
18 0 0.819 1.296 1.503 1.51 1.352 1.543
0
21 0 0.883 1.39 1.553 1.569 1.476 1.589
0
24 0 0.942 1.446 1.555 1.594 1.523 1.606
0
27 0 0.992 1.483 1.602 1.611 1.567 1.623
0
30 0 1.032 1.517 1.61 1.62 1.583 1.631
0
33 0 1.066 1.538 1.619 1.62 1.599 1.64
0
36 0 1.099 1.561 1.628 1.62 1.608 1.64
0
39 0 1.125 1.569 1.628 1.62 1.616 1.64
0
42 0 1.148 1.577 1.628 1.62 1.625 1.64
0
45 0 1.148 1.585 1.628 1.62 1.625 1.64
0
 m-m curve plot W/O INHIBITOR
0.06 LB PLOT W/O INHIBITOR MSP
0.04 30
V 20 f(x) = 0.71x + 19.28
0.02 R = 1
1/V 10

0 0
0 0.1 0.2 0.3 0.4 0.5 0.6 1 2 3 4 5 6 7 8 9 10 11
[S] 1/[S]

RAW DATA WITH MSP

0 0. 0. 0. 0. 0. 0.
m 1 2 3 4 5 6
M m M m m m m
M m M M M M
bl 0 0 0 0 0 0 0
a
n
k
0 0 0. 0. 0. 0. 0. 0.
00 0 02 02 02 02
5 2 9 3 3 4
6
3 0 0. 0. 0. 0. 0. 0.
0 14 1 17 15 17 21
4 5 3 6 4 2
9
6 0 0. 0. 0. 0. 0. 0.
0 34 3 37 32 33 40
3 1 5 6 9 4
6
9 0 0. 0. 0. 0. 0. 0.
0 44 4 50 5 47 61
7 7 1 4
1 0 0. 0. 0. 0. 0. 0.
2 53 5 65 66 64 77
0 4 9 9 3 2 8
9
1 0 0. 0. 0. 0. 0. 0.
5 61 7 83 82 78 96
0 9 3 6 8 6 9
6
1 0 0. 0. 0. 0. 0. 1.
8 7 8 93 97 94 12
0 8 8 2 3 8
2
2 0 0. 0. 1. 1. 1. 1.
1 76 9 05 10 07 25
0 3 8 8 2 8 3
7
2 0 0. 1. 1. 1. 1. 1.
4 82 0 16 21 19 35
0 2 6 6 9 5 3
7
2 0 0. 1. 1. 1. 1. 1.
7 87 1 24 32 28 43
0 1 5 5 2 9 4
3 0 0. 1. 1. 1. 1. 1.
0 96 2 32 40 37 50
0 1 2 3 3 2
6
3 0 0. 1. 1. 1. 1. 1.
3 95 2 38 47 43 54
0 9 8 6 4 8 7
8
3 0 0. 1. 1. 1. 1. 1.
6 99 3 43 52 48 57
0 2 4 5 6 8 5
1
3 0 1. 1. 1. 1. 1. 1.
9 02 3 48 56 53 60
0 7 9 2 5 1 5
1
4 0 1. 1. 1. 1. 1. 1.
2 05 4 51 6 56 62
0 6 2 8 4 1
4
4 0 1. 1. 1. 1. 1. 1.
5 08 4 54 62 59 63
0 3 6 3 3 7

0m 0.1m 0.2m 0.3m 0.4m 0.5 0.6m

M M M M M mM M
0 0 0 0 0 0 0 0
30
CORRECTED DATA WITH MSP 0.13
0 0.13 0.13 0.14 0.1 0.18
9 3 4 3 51 8
60 0 V 0.33 1/[S] 1/V
0.29 0.34 0.30 0.3 0.38
8 6 3 16
90 0 0.44 0.44 0.47 0.47 0.4 0.58
2 4 2 7 51 6
12 0 0.52 0.57 0.63 0.64 0.6 0.75
0 9 3 19 4
15 0 0.61 0.71 0.80 0.80 0.7 0.94
0 4 7 5 63 5
18 0 0.69 0.85 0.90 0.94 0.9 1.10
0 5 6 9 9 2 4
21 0 0.75 0.96 1.02 1.07 1.0 1.22
0 8 1 9 9 55 9
24 0 0.81 1.04 1.13 1.19 1.1 1.32
0 7 1 7 6 72 9
27 0 0.86 1.12 1.21 1.29 1.2 1.41
0 6 4 6 9 66
30 0 0.95 1.2 1.29 1.38 1.3 1.47
0 6 4 47 8
33 0 0.95 1.26 1.35 1.45 1.4 1.52
0 4 2 7 1 15 3
36 0 0.98 1.31 1.40 1.50 1.4 1.55
0 7 5 6 3 65 1
39 0 1.02 1.36 1.45 1.54 1.5 1.58
0 2 5 3 2 08 1
42 0 1.05 1.39 1.48 1.57 1.5 1.59
0 1 8 9 7 41 7
45 0 1.07 1.43 1.51 1.6 1.5 1.61
0 8 4 1 7 3
[S]
0.1 0.034 10 29.12
33 941
0.2 0.037 5 26.38
904 253
0.4 0.040 2.5 24.76
388
0.6 0.042 1.666 23.50
548 667 261

LB PLOT MSP
35
30
f(x) = 0.64x + 22.87
25
R = 0.98
20
1/V
15
10
5
0
1 2 3 4 5 6 7 8 9 10 11

1/[S]

M-M CURVE MSP

0.05
0.04
0.04
0.03
0.03
V 0.02
0.02
0.01
0.01
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

[S]
 absorbance vs time
1.8

1.6

1.4

1.2

1
absorbance
0.8

0.6

0.4

0.2

0
0 50 100 150 200 250 300 350 400 450 500

time in secs

0mM 0.1mM 0.2mM 0.3mM 0.4mM 0.5mM 0.6mM

LB PLOT OF MSP(1mM) vs without inhibitor

250

200 f(x) = 11.72x + 91.67

R = 0.64

150

1/[v]
100

50

0
1 f(x) = 2 3 4 5 6 7 8 9 10 11
R = 0
1/[s]

Linear () Linear () Linear ()