HISTOLOGY PRELIMS FIRST

PRACTICALS: LYMPHATIC
SYSTEM
KRIZIAOUMO P. ORPIA, RMT, MED-1C
LYMPH NODE
 Cortex

Capsule

Medulla
CORTEX Trabecula

Trabecular Sinus

Subcapsular Sinus

Capsule

Mantle/Crescent
Germinal Center
of Flemming
Lymphatic Nodule
CORTEX Trabecula

Trabecular Sinus

Subcapsular Sinus

Germinal Center
GERMINAL CENTER

Located in the central region of the nodule and

appears lightly stained in histologic sections.
Developed when a lymphocyte that has
recognized an antigen returns to the nodule and
undergoes PROLIFERATION
AFFERENT ARTERIOLE
HILUM
Cortex
Medulla

Branch of the
Trabecular Artery

HILUM
STRUCTURES IN THE HILUM

Efferent Lymphatic vessels

Trabecular Artery
Trabecular Vein
 Branch of the
Trabecular Artery

MEDULLA
Medullary cord

Medullary sinus
 SIDENOTES
Medullary cord- an
extension of the the
paracortex in the
Medullary region
Where
Macrophages,
Plasma Cells, and
B-Lymphocytes
are harbored.
Medullary Sinus-
harbors granulocytes if
there is active infection
LYMPH NODES
Lymphatic Capillaries Blood Capillaries
More permeable Permeability Less permeable
Thin walled Thickness of lumen and Thick walled
wall
Present Presence of Valves none
Review Questions:
No pump, relies on body Pump Circulatory pump (relies
movement to move on the circulation
Chyle (Lymph) CONTENT RBC
Lymphocyte WBC
Lymphocyte
Platelets
 The leaflets of the valves are pointed towards the flow of Lymph
Layers/ Parts of the Lymph Node
CORTEX B-cells/Macrophages/APCs/Reticular cells
PARACORTEX T-cells CD4
MEDULLA B-cell/ plasma cells/ Macrophages
Primary Nodule B-cells
Diffuse Lymph Tissue Middle Recta Artery
Germinal Centers B-cells
Thymus T- cells
GERMINAL CENTER

When an APC returns to the Node with a recognized antigen, they form a germinal Center
Germinal Center of Flemming
Usually in the cortad
PATHOLOGY OF LYMPH NODES

Lymphagioma- malformation of lymph vessels

Lymphadenophathy- enlargement of lymph nodes
Elephantiasis- Wuchereria bancrofti- Lower Limb; Brugia malayi- Upper Limbs
Lymphadenitis- inflammation and infection of the lymph
Lymphangitis- erythema following the course of the lymphatics
TONSILS
Germinal Center

Tonsilar Crypt
 FAUCIAL- PALATINE T.
ADENOID- INF. PHARYNGEAL T.

Germinal Center

Lymphatic nodule

Stratified squamous
e.
- High erythrocyte content
- Sinuses with blood
- Usuallywithout afferent or efferent
- Accessory spleen

- Hemolymph node- intermediate form

HEMAL NODES
COMPONENTS OF THE WALDEYERS ING
WALDEYERS RING

Pharyngeal tonsil
Palatine Tonsil
Lingual Tonsil
Tubal tonsil
DIFFERENTIATION
PALATINE TONSIL LINGUAL TONSIL PHARYNGEAL TONSIL
Non-keratinized strat. Squamous Keratinized Strat. Squamous Respiratory Epithelium
10-20 tonsilar crypts One Crypt per tonsil No Crypts
 Epithelial lining tend to inflame
Because of a THIN epithelia

Germinal Center

Lymphatic nodule

Stratified squamous
e.
SPLEEN
LIEN
SPLEEN
Pointed is the CENTRAL ARTERY

In the White Pulp

Central artery is surrounded by

Lymphocytes collectively termed
As PALS or

PERIARTERIAL LYMPPHATIC
SHEATH
PERIPHERAL T
WHITE
PULP (MALPIGHIAN BODIES) WP MZ

WP- white pulp GC

RP- red pulp
GC- Germinal Center
MZ- Marginal Zone/ Mantle
T- Trabeculae

Central Artery

RP
SPLEEN

WP- white pulp WP

RP- red pulp
GC- Germinal Center
MZ- Marginal Zone/ Mantle
T- Trabeculae
T MZ

GC
TV- Trabecular Vein TV
T- Trabeculae
T
 GC

WP- white pulp

RP- red pulp
GC- Germinal Center
MZ- Marginal Zone/ Mantle
T- Trabeculae WP
RP
THYMUS
THYMUS
THYMUS
 THYMIC CORPUSCLES/HASSALLS CORPUSCLE

Concentrically arranged
epithelioreticular cells (Type VI)
that are isolated and closely
packed

Contain keratohyalin granules

IMPORTANCE OF THE THYMUS

A primary lymphoid organ, same with the bone marrow

It is the maturation site for T-cells or T-lymphocytes,
selectively classified as a T-Helper, T-regulatory or T-
Cytotoxic Cells
REFERENCE:

Histology: A Text and Atlas w/ Correlated Cell and Molecular Biology by Michael Ross
Bloom and Fawcett by Don Fawcett
SLIDE BOX 20: Photographed by Kriziaoumo P. Orpia, RMT, MED 1C

N.B. please be guided still with your own atlas.