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Description

StrainDesignation:[CBS1320,CCRC20583,IFO1234,NCTC4614,NCYC81,NRRLY973]
DepositedName:SaccharomycescerevisiaeHansen
ProductSheet ProductDescription:Anampoulecontainingviablecells(mayincludesporesandmycelia)suspendedin
cryoprotectant.
Saccharomycescerevisiae
(ATCC7752) Propagation

Theinformationrecommendedinthissectionistoassistusersinobtaininglivingculture(s)fortheirstudies.
Therecommendationdoesnotimplythattheconditionsorproceduresprovidedbelowareoptimum.
PleasereadthisFIRST Experiencedresearchersmayinitiatethegrowthofacultureintheirownway.

StorageTemp. ATCCMedium200:YMagarorYMbroth
Frozen:80Cor ATCCMedium1245:YEPD
colder ATCCMedium28:Emmons'modificationofSabouraud'sagar
FreezeDried:2C

to8C GrowthConditions
LiveCulture:See Temperature:30C
Propagation Atmosphere:Typicalaerobic
Section
RecommendedProcedure
Forfreezedry(lyophilized)ampoules:
1. Openanampouleaccordingtoenclosedinstructions.

BiosafetyLevel 2. Fromasingletesttubeofsteriledistilledwater(5to6mL),withdrawapproximately0.5to1.0mL
1 withasterilepipetteandapplydirectlytothepellet.Stirtoformasuspension.
3. Asepticallytransferthesuspensionbackintothetesttubeofsteriledistilledwater.
4. Letthetesttubesitatroomtemperature(25C)undisturbedforatleast2hourslonger(e.g.,
overnight)rehydrationmightincreaseviabilityofsomefungi.
IntendedUse
5. Mixthesuspensionwell.Useseveraldrops(ormakedilutionsifdesired)toinoculaterecommended
solidorliquidmedium.Includeacontrolthatreceivesnoinoculum.
Thisproductisintendedforresearchuseonly.Itisnot
6. Incubatetheinoculumatthepropagationconditionsrecommended.
intendedforanyanimalorhumantherapeuticor
7. Inspectforgrowthoftheinoculum/strainregularly.Thesignofviabilityisnoticeabletypicallyafter1to
diagnosticuse.
2daysofincubation.However,thetimenecessaryforsignificantgrowthwillvaryfromstrainto
strain.
CitationofStrain

Notes
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Saccharomycescerevisiae(ATCC7752)
Additional,updatedinformationonthisproductmaybeavailableontheATCCwebsiteatwww.atcc.org.

DNASequence

18SribosomalRNAgene,partialsequenceinternaltranscribedspacer1,5.8SribosomalRNAgene,
andinternaltranscribedspacer2,completesequenceand26SribosomalRNAgene,partialsequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTG
TTTTGGCAAGAGCATGAGAGCTTTTACTGGGCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTG
CGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGT
GCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTT
TTTCTTTGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAA
TTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCT
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGA
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT
CCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCAT
TGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGG
TTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTC
AmericanTypeCultureCollection
TAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATC
POBox1549
Manassas,VA20108USA
AATAA
www.atcc.org
D1D2regionofthe26SribosomalRNAgene
800.638.6597or703.365.2700 ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAA
Fax:703.365.2750
AGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGT
Email:Tech@atcc.org

TCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGT
Orcontactyourlocaldistributor TCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCAT
CTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACT
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TTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTT
TTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAG
GATAAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGA
CTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC

ProductSheet Isolation

Saccharomycescerevisiae GebruderMayeryeast

(ATCC7752)
References

Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
PleasereadthisFIRST
BiosafetyLevel:1
StorageTemp.
Frozen:80Cor Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
colder thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
FreezeDried:2C DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes

to8C forHealth.

LiveCulture:See
ATCCWarranty
Propagation
Section
ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
BiosafetyLevel effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein

1 mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.

IntendedUse
Disclaimers

Thisproductisintendedforresearchuseonly.Itisnot
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
intendedforanyanimalorhumantherapeuticor
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
diagnosticuse.
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
CitationofStrain confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
Ifuseofthiscultureresultsinascientificpublication,it
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
shouldbecitedinthatmanuscriptinthefollowing
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
manner:Saccharomycescerevisiae(ATCC7752)
themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2017.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/06]

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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