CFX96 Touch” and CFX96 Touch Deep Well” Real-Time PCR Detection Systems Installation Quick Guide Unpacking the Instruments “Tho GFXO8" of CFXQ6 Deoo Wet” optcalreacten modu and he tooo Tous" smal jr chas ar siopd near cersaner Uroack sch boxcar. 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This acton moves the reaction module orwar, lasehg ino lace Pig {yh oreemoate oot on 6. Usotha supped power cad plo the casas into an sonreeite sect ot. 6. Timon G06 Touch er CP Touch Deep We eatin eg te power sch en ebackot be hase, ‘Removing the Red Shipping Sorew “The CFXO8 an CF%O6 Dogo Wel ontcl acon males te stippod th ae serow place nbs iear Kin the hole coresancng wal At (G00 Fur 4 Flow the nsructon in tha C1000 Touch seen tronove ‘ie shpang sren belo begining operation.Operating under Software Control Installing CFX Manager Software ‘Roce wn be WindowsXP, Wiedows 7, Windows 8cprsing sts seated tora the stare Menu camer soca se lsad ne OPS Touch CFAGE Toth Dep Wl, (GPx Cannes ond CPGH4 Touch sate retucion manual. The etasremmus be nstaled ‘nine complet ure wih acrsiaho ogc, To ntl CPX Manager soar 1. Disonneo yrs netument rm the coment. 2, Pen the CFX Manager softar® CD inthe comput’ CD sive. The sotwaelaunch p98 ‘sods appear autora. 4. Dau ick neal Software cn the oftware aurch pgs. 4. Fal theinseuctons onscreen to corpo staton. ‘5. the unch page does nt appear automaticaly. doce cick (2D eve Bio-Rad CPX, ‘ten ope and low nseucns in th Roasts Instaling the Devers th nsrumert powered of tg the suppiad USB cabana USB B port ge 9 ose ‘ont bok ol ne C1000 Touch asa ad nto a USB 2.0 A pot ent comput. Ta nthe {GEx86 Touch er CPG Teveh Dep Wel am ug the omer switch on he back of he Css. = youre ving Windows 7 or Windows 8 eparae str, the vers Ww be ‘anomaly retalod + yous using 8 Wdows¥P operating ston, flow he deat apions nthe Found New eonare Ward ht launchs after te nsrurr ort dtocte by the computer ‘ter irtalaton — opan CF Manage eotar by cube chr te ‘Storeutenn oatcen ro Winows esky [rthe Datei Insert pane lhe man CPX Manager soars window a sstom as ‘ot appear inhi pane, cant proper USB connection a ental th orb sling ‘Toole > Reinstall instrument Drivers am ema satwarewirdow mars Ns ‘Starting and Analyzing @ Run “The un Sta window provides quik areas tthe i and stings neaded ose Up sraatart aan To open he Fun Setup winon flow one cf tase ops 1 Gi he User defined or PrimePGR buitonh tho Fu Sup ab ote tatup Wears + Clk th User-defined Run Setup PrimePCR Run Sat bastcnin hers stare tober + Ssloct Fila» New'> User defined Run ot PrinePCR Run otha man sotware window ‘Tha Rin Setup window ncies te tabs ure Se + Cle he Prtaeal abo select an tng proc ounce to rat a ne potool Inthe Proto Ester window 1+ Glen Pate tab 0 sole an exng ato ton or et, oo eee new ple nthe Pata Ester wow + Cickne Start Run to chack in stings set oe o mre instrument leks, andbogn ann Fer PrimePCR™ ins, the PimePOR proceso deat lt you wil bs elect based one Ineo elect inh Run Stipa. The PrngPCR proood nds met ep by default, ‘oreduco earn ta, sect he Protocol ta and uncheck be at tap box (crx Manager ctwars automaticaly process rain PCR cat een of ach na and ‘pane te Data Arai window. Te data canbe any or saved to th edo four hole, fd can bo emale tem te twas. fr ho OPXOE Touch, CFG Tourh Deep Wel, (Cex Comat and CH84 Tavoh sytens suction mau dale evomaton abt run ‘up ana cata anal) ‘Shoo tenn chase paueas‘Stand-Alone Operation ‘The GF396 Touch ae CFAGS Touch Deep Wel systems can aso be operated a stan-alone touch-screen nsrumets that can run area's POR protec! without a computer tached. Howe, data asi eres he ve of CFX Manogr sofware nstaled on aPC: Creating a Protocol + "Tocree raw un, uch New Protecat nha hore seen to open a Naw Proto tape (Fer0 7 touch tho Name fod hen ere aprotcet nao fo S00 + Toedt an axsing petced euch Saved Files ocpan tefl raya colt a protec owt + Former nforaion abet creating, edna, ad uming too, ero he PAGS Touch (C106 Touch Doep Wl, OFX Cormac, and OFM Teh jms Mercer marl Exporting Data When anand 8 fuessoans data neo ba rated a compute ring (CFKManagersoftare for ana. he ed of ar he dats a sutra save the Fea-Tme Data fer icaed the Ra Te Data crectery Figure, Upto 100 rel ine PCA, rs aestoredin his er Exporting Data Using a USB Flach Drive + fa USB han eres prosnt in 8 USB port on th C1000 Touch chasis athe end ofarun ‘th deta (2 wl autratcaly bo saved oto Fa/-Tino Data od, whch wl be ces ‘no USB Hash ave + a.US8 tach cre has nt been sored to @ USS por, srt anes el tase cons ‘oranda ato be USS fash ve: 1. Touch Saved Files th hore srbn oss the Fle srs 2 Inte Locations pane, sect Real-Time Data, 23, Sct lt export om the Fes panekfeeaten abou the sects ibs ‘layed in ho review pare 4, oespor te, touch ne File Options ute, '5, Tae the OK button o sath othe sts US fash eve. Exporting Data Using Email ‘Ya em choose to mal yur data tom he C1000 Touch chassis tr contin your sal tating (ete eting Up maton the GY000 Touch Chass soto nts ck a8 To snd an ama whan ttachod dato (2p0 fo the C1000 Touch chasis at the endo an, eter an eral adress th Atona Stings window iro theca sat Poe Note ina usrs ar logge, thy can spec hat anafcation and an alachent be sett prods erah aden athe comploton ct eae, tants Fe saecgemacntetonAnalyzing a Data File A stance un data (2p must bo converted ho a data ana fo (pcr by OFX Manager ‘eiteaein ade tobe anahzed.Cranea cla anayasNerom a tan.lone un using ee oe talonng opts: + Chek ana ag na zr vert tha man sofware wow Sl Fle > Open > Stand-alone Run fer he man stare wow menu epions ‘ho softwar wi clot a data lt tht matches the scan mode ard pate size ard appy tt ho tance rn dt lo creat 9 CFX Mager str data pe. The te wl hen open Intra Data Araya wow anc ary pata layout ets can a made by saecing he Pate Setup "ton nthe ota: ler to the CFXO8 Tous, CPAGS Touch op Wl, FX Conc, and CFXG84 Tou sjtome nsrucion manual fr deta ermaton out data ara Setting Up Email on the C1000 Touch Chassis “To canteure he outage rom the C1000 Teuch chess, low these nstructons: 1. yeu nae not aeady done 6, te nome sre, se Took, hen ouch te Admin ution toleg astra acinar. no password asbosn sa he Aaran oasis appears wihot login prompt 2 Inte Adin too a slot mal Settings Fgura 10h §. Sect «mal ere fom he depsoun manu nthe ugg Mal SMTP) Sore Sotings window or ereata anew on by outing the New Server ten go Ti Mako this cover ‘hed by ouching ha Set As Default box 4. To rate an mal sone, ete ho ma ser aks and mal ane porta approne fais. Aneowerk acini may need o be contacted is obtain is momen. ‘5. ner th use are and pasouerd fr he selected ma sever 6. oven net me Uutgong Mal (HH) Sever Stings ar covet, a tat sal canbe sent bouche Test Ema tor. 7. Err the emai aces and an attachment ie, then tc Sand Ema Fir 12) Sect anatn nonowned US pr tne ar cr Sn A ee @ CED 2 nn Upson RR Sn en Sener Somer anaes Sean eee meee eee ‘Gases nara ee a eee SU Ta ar usCe ley Cree Teal g Quick Guide Combine he unsurpassed peromance st ‘oxy of OFXO6 Touch” CFXB6 Touch Deep Wat, CFX Cannoe, or CPG Toe” reattine PCR deacon syst wen inpressh ‘sajoatoak in abate sorters, Athens asters ‘ame wth alioonge fo abasoe software. Tas ‘oer combination wt speed up your lime FOR epormonts and aca cory. ose+ ithe protessorlsuocossor ofthe Ghort Usiveraty Base stare frre PCR cata ‘aaj. chaser lstabed en tha prove shor ‘nd fan ncnology (ith hound fans PubMed) and erranced win prepay ager _ndinnovave fers. Easly et youre taro bases fr furar nahi. Labases Software License ‘ourvimave aban sttore bene action ove proved on he star below. The caso Icenseis unique and sect ra given computer Bogezslshas a absco vrs fr al male, compute cperaing toms, euch as ‘Miosot Windows, Macros, and rx “Software License Activation Code Biogazelle qbase- activation code \VuLhbg6N Register fora Biogazele Account |. Non wir blogazele;com, 2. Clk Login the Biogzate home page ‘lok Greate now account yBiogazaa ogn windon Fue [Login myscpazete Fg. nlognt ogni 28 Prov ser deals and use conact -rermation Cis Create new account Activate Code 1 Ck MyBlogazale the hon o8ae ‘whl loged 2.Cick Licenses. Cick Use activation ode (Fgue a2. myencte tense wacom {8 €rtar the actaton code proc by ‘Bo-RasL orto, and i AtivateDownloed qbase+ Software 1 Ck tho Software downloads sone MySogaza10 window que. 2. 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Now you es redy to uso qbaso+ star, An ort _ghases Sortware Information Formor iterate abou soot cotare, ‘www biogazele.com/gbaseplus For super login to your account, cick Support inthe Sioa ame pape, than cick Submit tekst You nay dco contac: ‘ourlocal Bio-Rad supper ra or more rain, Srebriteeceeme erence ne For mare intron aut CPX Manager softwar vist wrnbio-ad.comvampSWabaseactChecking Firmware and Software Updates RRR Mur Ronee Uru) (CFX96 Touch”, CFX96 Touch Deep Well, and Adngahiaede Real-Time PCR Detection Systems v3 Bio-Rad Laboratories, no, portal fers upates for C1000 Touch thermal, CFXOE Teuch syst, CFAO8 Touch dopa ate, and FAA ‘Touch tom fare ana ie OPx Manager Sortware Wo song recarmend tat you rogdary ‘heck our aytem anita any vate ups betang Updates aio opti roar ors you instant and provides aera eahos as "hey become mii, ‘C1000 Taveh heal yea the GPE Touch, (GPA98 Tun deap wal oF CPXSBE Touch 6m and the vron of CF Manage softwares ‘you computer Compare heoo otal ‘eros peed onto Bo Fad wabsto at ‘wow bieradcomper updates {Tum he nner; wat un the tens 2.0n he mal menu srs fhe 1000 Toc ‘hormal lr touch he Toles gue Touch About nth Use menu gue 2. 4. Compare thesia frre ersion dpiayed onthe insburont gus 9 wth ho most curont Note: Te 6051 ane DSP frre se uses ony forreltime POR. youare us @ C1000 Touch thsma cir for standard PCR, tec rmware Efe ranemarepan amr soramsten6. the version fsa on your nstrurant does rt math ‘necurent vate Ietedon the websta, dined nd Insta th ute ‘Checking the CFX Manager Software Version Installed 1. Launch OFX Manager soar, 2, Sot Help» About Yom to man sorters ‘window Fue 3. The About o-Rad CFX Manager window depays the otal eofvare version (aie 4. he version tale on your eur dos nt match the cunt version std on te web, dowrload and ae upda Note: CF% Manager sofware version 3. can automatically hock {orp th computer comet ote sme For mere "emai rear the CFXOG Touch, CFXO® Teuch des We (GFX Comsat, and C214 Toh eatine POF detection systema ratusion manual eset amon ceintmor ios hots CED Bes ne 4 Fo. Nping er wo of CP Manage te ——— Bio-Rad CFX Manager 3 i 5 ated atari payed nt Au Rada ce)‘un Setup Protoco! Tab “ha Protea ta lea @xovaw fhe ectoc loscedin ne Fan Step wow Fg 1 ‘Gc Create Now open ho Proto tor tmoeataa new prion. (Gick Stect Existing to unc eso browser ‘load aprteel ton or ost Use th Exess Lad ropiown mento dct lead probe tonmartoedt (ick at Sotcted to open ho Protos tr tweatthestaps afte soleted toca, ‘hoc th Start Run a ota an th the ‘erent oaded preteal protocol or ea an auzing ono igue 2, "Slot any tp eta to graphical or txt ‘lay ~ to sto bocomse Nghigheds| ue. lek ne roar or aa ne recy eat ro vale 2 Ck ner Step 0 aa tmporate step to ‘he protcok ck Daete Stop to romove ahghigied Step tom he poo, 8.0 Add Plate Read to Stop o sige "ahentuerescance cata wl be eccueed cing ‘hegre Th ext onthe ton charges ‘0 Remo Pate Pod ho cureny ighighiod sop has a lat red. yu do nt wae acqure daa at het tp, an cc Remove Plate Read 4. Chek the umber of rapats ofa GOTO top 1a change the nur oF jes he potocol Fawe2, Sep 4) (lk the GOTO stp number to cheng ‘he stpsncidd inthe GOTO on, fe GE larl: (tae ed Lhasa me aR cd 5 reac bint Fan ag den sd arg peter ome ip. 2. rea Ete pot erg ar one [Stn ence ten Sci rons Sap ie Npcras a soe sums digs Poa oa“oa grace step coor tet 1. Ciek insert Gradient inna protocl ext contol que 2. 2. Tot ra temparaue ves for thalowest ard ghee peratures into gracin, lek ihe ‘elise nth phil pay, nto txt Seta, ‘rin the radontango calle hat appears Totheright ot te ted doplay Flaue . 8. Win th grant range calor, pect temperature canbe asalgod to ay row Echrow than popu wih the appropiate verpareue satay the pected range. 1950 ¢ tr300 ig. eae crncer sep nie Proc Eten Ase os haus scumugaon tna aaron Tognee Toads arma curve 1. Clk Insert Matt Curve nthe preoco eng com Faure 2 2 tho gaphical or txt pay, ck ne tonperaro vais to eat ha lowest anc Highest temperate of hema case ange (Paws 4 Sp 5, 2. lek he herent ue to et he trperatre ‘sana at whch cota are anaures 4. loka cial ata et th ination te fer ach torpertr inreert Fo. c iar Gnesi ba Pcl Eat: Ave ieos ‘Sigmcntue nb pastas stat ope secre lo tars to ara oe sry caer tang US. carat fronts ony Oe A CED =... —“ Eee eoCFX Manager” Software Plate Quick Guide fun Sotup Plato Tab “Te Piet depo a prodow copa loaded nthe Fun Soup wiedow Fig) Click Create New o aoe ha Fate Etro cat a new pt Click Select Exlating to lunch the te bowser oad a plate fletouse narinorto edt seth Sxprens Load cropdoun manu te cet oad a pste fletouss narunorto eat Click Blt Selected to pn te Pe Etro whe vl covers of he soto late (ick to Start Run ab to proosed and tart aun with he curanty losdod ats “ho la Eatoris oad to creat @ awa oe an exiting one Fa. |. Use tha Scan Mace cropdonn manu nthe Pt Eton ‘ecb to deignae the dts ecquaton mado be sed rng then 2.Cltk Select Fuorophores ona the Msrophres a wie used he run ‘8.mitin pata gra slst ova oa, 4.Choom he Serpe stam dap men ‘5.Clck the appcpriate cracker ola the heropheres) inthe selected wal 6-Tpe the Target Nama foreach raphe fue fa gene xpress aaj) and ros Enter cr choose on ‘me cropdoun mer, 1. Tpathe Sample Nas fequed fr gan axvession ana) peas Enter, cr choos cne on fe cepdoan men. |. To ertarta Bllogioa Set Nam, check Blloglal Seth ‘he Vow bax ho baton of he data ara window. ‘For gona wession ana, cick Experiment Settings to sssrrence target and cant sap BIO. Fa. Fmubinee rin Seip wdon tensa pe ‘beste mepeecaaconass oer oeat et ane‘eer Rept ambos sexing came ‘ocdeagpata ast ofrepioste wal, ighight he wels sna ‘ype. choot a opcate number he Repeats # box in be Pate ein cones gue Ateneo asin repos haar srl wel subsets toes 1. Sot vin plat agra ack Rapicate Salas, “Te epsta Saves cg wndow opens Fg 2. the Replicate Group Sie andthe Starting Feplesto# 2 Inoat whether repos ee loaded horaotaly or vera 4.Clck Apply to era the repost umber retin x Standard re "oertar ie staring target concertaton of ne sand ‘alt he wets loaded wa Sate Ta/Stardar, ora ‘al under Caneaneaton inh plate ding oon Faure, ‘sgt Alera spec turophere and than cick ths Load checkbox tate, tr concertos fore ere ‘Sandard une aia at ence. |. Salt re wal hatha alo oan assigned consecutive replete numbers and lex Dilution Series. The Dizon ‘Series window opens gue 4 2 Ener the Staring Concntaton of he dition sais, Ese tho umber ofthe rt and lst rplentos inthe seis {ter tne Dlaton Factor en nea wher heaton ie ‘remain or decossng (hil, ha he aus Orion ig 4 Din St widow a eat tna ‘Step 28 tha leat or ghest concanatin, 5.Clck Apply to sssign helo sees crest Wel rune To creas wal grape hat are anaes ndopendorty 1. Ofek ne Wall Groups button in he Pats Etta. “The Wel Groups Managorwhdow cpons Figs 5, 2.Clek Ad to crest ne group, ‘nthe late gram, salt ha was at wil const the ‘wal eu, 4.Clek OK totum tothe Pte str wn ‘aaa natal eta nd ner nowt on Genet ge cameo suas oprah sae Forma tration, vit wr.bio-ae.comampSWBEPIat, CED si, 'e q ‘t iy i i 4 4 I‘Quantification Tab nein ‘The amplileaton chart pay re of he lave uersoenoe cote fem each wel at very cyl ot thea (Choose the orophore data you went opis by clkng te ‘horoprere checkboxes etd ura ho areiceten chart Foue Clee was, ro, cluresin he wal adie Figs) te doxay turescence race and afer ssid wal Selctad wal ar ue; unseated wale are ht gy. Poston the cursor ora uerescarcs tach ovr a wel trowel sdecter Figure 1, wt AS, ver aca nthe cata Sreashons cre poi on the standard creo ight earespondng rrmatenm a furlocstens. ‘Tho sete uses Wo modes cuareneaton gyo~ Co) starr regesacn snd age aera, Sao Settings from to mona an hen colt Ga Determination Mode ‘bchange ta deerinaton mate. nthe Sng Teeseld ‘modo, lekand cag the troshold ine to aul poston (Cex Manage ota automata subtacts he baslne from wa data. Seet Settings fom theme br and tron ‘Slat Baseline Threshold o change he basen ange ft anywol Clcktho Plate Setup burton o chose: + View/Eat Plate — et wet corer, crest we ups nda, rom, crexcrse wae fom ane + Replace Pate Fle — crangezlate out that of ‘sproviusy sed pate fla), + Apply PrmePCA Fle — charge pao leouto hat ot ‘a PrePOR™ nn fos) BIO-RAD CFX Manager™ Software Data Analysis Quick Guide 1 uanteson nna Daa An nan hone SSschernieee ements eons a2 Popp mannan Hcg he ‘Selecalonoe ‘ar optone Fight clek any char to o2py or save ho rage, ‘ri elect Chart Opting to mod he chrt Fave 2, ‘Sait Trave Style tom the Setings ns ‘ight lek any chart cutis th race oles tor spec wae Lot tokary at hen ks dow ane rag theUse i Slat Wal Group ropown manu on th feabar view ata or a pect wo group gute 3. The swat wal (yocps a naependontrans, an eac can be aaized wi its ‘em setings an, appleabia wih own standard ce (lone Manage Well @roupsiconontetooberto sas ormocty wel un ‘Quantification Data Tab ity Optens Ciccthe Quantification Data she Daa Anas wow 1p ou numeral aia edeuators aed sats, incuang C3, ‘or eat alan the ean and tara dvi or repestes awe 4, ‘Ghar column header to sot ous basd on at elun, Fight ck te sreacenet and elect Sort sot rows based ‘onmisecoitns. Choose Sect Columns to spec which ‘olan wile dapayes Lat ek any cote nase and rahe column oho to ‘tha gt to rearrange the rer of th colannsin he spreads Fight ck te spreacohet and clot © expr ita as att, 20M, Mirodat Exo er HTML He Fgu Selec Plat from the copdown menu under he Quaicaton Date abo spl wel ia for a sdoctod tuoophore in plato sd format {creating a Report ‘Soc Tate > Reports fon to txtbar menu of he Daa Anas nd Pagur ) open the Report window Que) ‘Scr na Cock chackbows inthe report options once pace semi intomatoninherepor. win 8 dsplayedintho prefew wow 3 a ave. Eifion, Te orig of cao an os win areca Tectugel by chy rd gna teow be cated ‘st poston tale nto toba ad sot Sve os er ‘SarOro sect Pita ptr ena Save tho report telat for uso wth thr data les desk, ‘nic ae ctr oxen Sgr So fuch ewe salaries am conmabyone rosetta ete aztec oeroenrenrpare owes oeiorsovhos Pama ae 6 CHAZD 2... ys Sotence Sore Epa pepe laCFX Manager™ Software Gene Expression Analysis Quick Guide Win stigonty quad con, you can use the Gene Expression Mooue of OPK Manager software to wate rave clrences target’ conceration between sarees. The most common usec ths applet or ‘luting the target corcervaten Fh cONA sareas or steady ctata MANA lv. Type, ho meSsap Ios of {ner more reeenceganes is sed to porate the elves ofa gneofrites. elsence genes correc flag Gterncos or tar sapling varie precetn aac sample. The xrasson laf reranco gongs sherk ‘ary acroes th ila contin bang stuck, ‘Gone Exprossion Analysis Sotup “The xara wel shown Fig cnt ange furoghoeloadedn the wal wih fourepeta grouos cottanirg tw efron target rare and we deren sapiens, alning Rterence nes 1 cick Experiment Settings nto Pst Eo: or tho Bar Chart ao he Gare Expression Modis Tho sparmene Setngs window open QUE 2. Seloctne Target ab, 2. Cickine appropiate crocabone occa he tele that wl be vad as rlrenoes, 4. Clk tho Show Analyte Settings checkbox in eter ‘0 usa react felony oer han 100% or ary age 5. The Auto Ecleney chacbox's checked by dau. a ‘#arcar uve nas boon nccod har than he stfeioneyeaclate rom the standard curve wl be used Inne csledatora, Atoratioy ype aasston stfeency ‘luo imo the ol corseponding tthe approosat target ‘o ue his eicenayin ne clos, 6, Cheek tho sap typ) that shouts be exci tom gore axpeedon cautions. Note: Cranes nsclancy madein the bar cha setings window wl berefecedin the valuscalodsedn athe ig. Aniging teen rot ‘her pot p28airing Cote Somes ‘Tine Exprmant Stings wow, select he Samples tb. 2 Olek the aoeroprite checkbox teats tho samp that wl uses ashe coeain tho calclsons gu, The cote samgle i stigned aval ot fo every ge, wheal ator Sales are proses wit alos eat the cont Note: Te ort samp and rfrences can ato bo salectod tang the Getun Wears, which alalen th tg Tools ‘ronson maruin te Pt Ear window ‘Gone Expression Analy ‘Toro we soveral wast Vow gone oxresion dit: bar char, caustayan, sata p, volcano plo ad ht map. These otons ‘be slate tem ats on eet oof th anaes wow gure 4, Th Rest sb combines gone een ‘lectins rom ihe ferent pls no-one wri. (Gee expression eprosenacby bas of varying heat depending ‘on tele of exeroson fer ach sare ard taatcaranatn. ‘lc tn analysis mode tom the Mace cropdown menu (Figue 5: [|Z omweam | il ammeemll == Soo + Nomataos expression AAC — rave quanti of geno crerecticnomaladto ristve qustiy othe resence (ones aoc eames + Ralave quantity Ca — elas quay of gor ot erost | x Isnet nmalzc the ecu xpress to gone concerraion o ‘iste tacthersompina the apotinene ‘xaphing optens bar chars: + Select tom he Graph Data ceeponn manu Figure 6) depy ‘raph data reatve fo conta oginaing st er raat to ze Inte aa he Graph Data cpten cats 0 alae to ont + Clcthe Show Chert Settings box sc salt tom the o = Ss 2 Xi copsonn mana ie 0 Sly Sao Teast | onto xan * Selec rom tn VAs doosom menu (gue 1 ue Linas, —______lil o92, org 10 ashe y-au orale 4 Gre moan sami ctor abe + When poded, sc tom th Sclng ceopdown menu — Hahost, Lowes or Unsealed + 80cm he ior Tipe sropdown menu to slay ‘Stand er ofthe Moan cr Standard Dvaton + Groce the Sort ton on the tk raph menu Fgue 5) ‘tw oustonze the presertaten err 2 ha eis + Fgh lhe graph ony an past tno car aplestens ‘cto save copy he rach as an ago, Anata ‘conyng option to cek te ean nha top ght carro fat and rag to @ Mcrsot Word or PowerPoint countterra Samples and targets re clustered hear base on the sinlaly ara gana expresen. The data pont olor cats (stor sossen fo ower exresin (ren, or no change Ireesaon ck, Thoth ha cor, greater the xpresson i Graphing opens for dusters: * See ekteor abe deta by tga, sample bat get sau, o nate target ner cami by chooana fom {hota optons inh aprepriat ropdoem mena * Aust tna sia ofthe pot mage usng to Si sider + By coat, verano vais ae pot ody vais fr replete, sc Spt Ot Replicas ‘Tha eave dfrenc in expression of atarget or a.cona ‘susan experiential sample is displyed. 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