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Plant-tc Monthly Archive - January, 2006

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Re: MnSO4.7H2O and MnSO4.H2O


Subject: Re: MnSO4.7H2O and MnSO4.H2O
From: Andrew Smith <vancusmith@GMAIL.COM>
Date: Sun, 8 Jan 2006 22:48:11 +0500
Reply-to: Plant Tissue Culture <PLANT-TC@LISTS.UMN.EDU>

Hi every one

thanks a lot for the detail reply, I am summarizing all the mails I have
received

so its mean I can use MnSO4.H2O instead of MnSO4.4H2O


and what should be the amount I have to use in one litter of MS media.

Thanks
Andrew Smith

++++1-As far as I know, MnSO4 crystallizes with only one H2O... so the only
compound you could find is MnSO4. H2O.
++++2-Answer to be found in Kyte & Kleyn, p. 62.
++++3-They are the same except for waters of hydration. Check in the original
paper which one was used and then equimolar amounts of whatever you have .

4-1gram MnSO4.H2O contains 0,32505gram Mn


1gram MnSO4.7H2O contains 0,19823gram Mn.

5- This is referred to as 'waters of hydration'. You just need to calculate


how much of MnSO4 is present, excluding the chemically attached water. This
is a classic example which is discussed in several books, including 'Plants
>From Test Tubes' by Kyte and Kleyn (p. 62 of the third edition).
The following is for those who don't have a copy of the above book
available.

The molecular weight of MNSO4 without water is 151.


The molecular weight of MnSO4 . 4H2O is 223 (151 + 4 molecules of water 72). So 2.23g of this gives you 1.51g. of MnSO4.
The molecular weight of MnSO4 . H2O is 169 (151 + 1 molecule of water = 18).
So 1.69g of this gives you the same 1.51g. of MnSO4.

Simple ratios give you the answer:

If you have MnSO4 . 4H2O:


(223 / 169) X Grams of MnSO4 . H2O in the formula
= Grams of MnSO4 . 4H2O you need to substitute

If you have MnSO4 . H2O:


(169 / 223) X Grams of MnSO4 . 4H2O in the formula
= Grams of MnSO4 . H2O you need to substitute

++++6- Both of the substances you have (MnSO4.7H2O and


> MnSO4.H2O) are water crystal hydrates of one salt:
MnSO4. They differ each other only with the number of
water molecules which present in the crystal. In the
composition of MS it is important how many molecules
of Mn (2+) and SO4 (2-) ions you'll add rather than
water molecules. So, it is easy for you because you
just have to re-calculate the amount of MnSO4.7H2O
having in mind 7 molecules of water (7x186). For
MnSO4.4H2O you have 4 water molecules 4x18r. There
is no problem to use any of them.

On 1/8/06, Andrew Smith <vancusmith@gmail.com> wrote:


>
> *Hi Every one*
> *in some book i found MS media with MnSO4.4H2O*
> *and in some research papers i found MnSO4.H2O*
> *so please help me which of the following is suitable for MS "MnSO4.7H2Oand
> MnSO4.H2O"*
> *and what is the difference b/w them and what will be the problem if i

converted by W eb2PDFConvert.com
> use any of them *
> *please*
> *Andrew*

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