Salmonella

Day

Alternative Solution to Improve Gut Function Integrity

Dr: Nguyn Vn M - Vit Nam

Phone: +84986640965
Email: Vanmyayurvet@gmail.com
Factors affecting Productivity of Chickens
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Breed

Feed Disease

Management Environment

But the Big Questions are
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My Breed is Good My Feed is Good

I Control Environment
My Vaccine is Good nicely

What do they think about reasons??
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By Hand Ghost and Monster

Destiny

But the reason lies

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Which are the most important organs inside the chicken

Most important Organs in Digestive and Immune System

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Liver Intestine

Unfortunately
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Salmonella Spp Damage to Gut, Lung and specially to Liver

Infect to the blood and move to other organ like Lung and Lymphoid

Other pathogen like E.coli, Clostridium Perfringens, Histomonas etc. damage
other organ too

Even using antibiotics also affects the liver and kidney

Liver, Kidney and intestine are the target of pathogens

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Salmonella Clostridium

E.coli Histomonas

Mycotoxins Antibiotics

Enteritis / Diarrhoea
/
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Disease of Small intestine that destroys gut wall

 The Need....
In pursuit to replacement of Gut Acting antibiotics www.ayurvet.com

Bacteria / Virus Exposure Damage Tight Jn Inflamed Intestine

/

Intestine Malfunction Villi Reduction Poor Absorption Pathogenic Bacteria

Increases

Main Bacteria

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Salmonella

E.coli

Clostridium P

Coccidia

Concern about salmonellosis

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This Bacteria very common, and can infect between human and
animal

Bacteria causes most of food borne disease

Very dangerous: Kill a lot of human, animal, difficulty to recover

Look at this cycle

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 Where are we now?

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We are Here !

What is Salmonella Spp?

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Classification
Enterobacteria Aero- anaerobic facultative
-
Gram Negative

Several Species

S. enterica subspecies enterica, Serovars, Salmonella gallinarium,
salmonella typhimurium

All motile except S. gallinarum

Classification

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Species S. entrica > 2400 Serovars

->2400
Classification of serovars according to capsid (O) and flagella (H)

Ex Salmonella enterica typhimurium

Salmonella enterica enterititis

Salmonella Spp infects intestine most frequently
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Very commonly found in Environment and other species

Why dangerous damage liver, spleen, swelling in colon
membranes, damage immunity
-
Loss of profits

Resistant with lot of antibiotics

Very difficult to prevent and treat

Mechanism of Salmonella Infection to intestine in Chicken
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From Chick to Chick From Mother to Egg

From Environment

This is mechanism of Salmonella infection to intestine of chicken

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This is mechanism of Salmonella infection to intestine of chicken

Continued.. www.ayurvet.com

Damage gut

Phagocyte Blood vessel

Results
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Chicks - S. Gallinarium Pullorum

-
Clinical Signs

S. Typhimurium on chicks www.ayurvet.com

Persistance of yolk sac (+discoloration),

inflammation of caeca, hepatic necrosis,
perihepatitis, Pericarditis


Spleen
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Layer
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Fowl Typhoid: Caused by Salmonella gallinarum

Drop in egg production

Diarrhoea

Liver degeneration

Hepatitis and discoloration

Enlarged spleen

Layer
Continued www.ayurvet.com
Layer
Continued www.ayurvet.com
Layer
Continued www.ayurvet.com
Layer
Continued www.ayurvet.com
Results of Antibiotic Resistance of Salmonella

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MIC of Salmonella spp. (2016)
Salmonella spp. (Group D) 2015
250 2016
206.5
Concentration (ppm)

200

150
POULTRY
122.7 122.7
105.1
100 86.5 88.6
71.6 76.1

50 32.1 37.9
16.0
0
Amo Amp Col Dox Flo Nor Hal Spe Gen Tri Enro
2015 Amo Amp Col Dox Flo Nor Hal Spe Gen Tri Enro
Mean 170.67 149.33 57.72 64.00 59.33 25.9 36.4 64.0 6.0 160.0 0.5
Min 128.0 128.0 1.0 32.0 4.0 1.0 16.0 64.0 4.0 64.0 0.5
Max 256.0 256.0 128.0 128.0 128.0 128.0 64.0 64.0 8.0 256.0 0.5
No. of samples 6 6 6 6 6 6 6 2 2 2 2
2016 Amo Amp Col Dox Flo Nor Hal Spe Gen Tri Enro
Mean 122.7 122.7 86.5 71.6 88.6 32.1 16.0 105.1 76.1 206.5 37.9
Min 16.0 16.0 2.0 32.0 4.0 0.3 8.0 32.0 2.0 16.0 0.3
Max 128.0 128.0 128.0 128.0 128.0 128.0 32.0 128.0 256.0 256.0 128.0
No. of samples 21 21 21 21 21 21 21 21 21 21 21
2015 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
No. of samples 3 1 1 15 1
Most Popular method at this moment in prevention and
Treatment
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Traditional Method

Antibiotic: Enrofloxacin, Glucozo, Electrolyte, Use Liver tonic after

Amoxicilin, Gentamycin, Vitamin, B-complex
Tiamulin, Florfenicol treatment


B

Step 1 Step 2 Step 3

1 2 3
Advantage of Traditional Method

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Good control and fast symptoms of the disease

Cheap price, convenient to use and apply

Increase animal health, increase body weigh, optimum FCR

Disadvantage of this method

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Antibiotic kill all bacteria: Beneficial bacteria and harmful bacteria

Do not protect Gastro Intestinal membrane

Increase more Endotoxin because kill all harmful microorganism
and can not absorb and neutralize Toxin

Can not support for Villi growth and mucus production

Develop more resistant bacteria

Special

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Takes a long time to recover GI and body weight

When the liver is in necrosis cause by Salmonella Spp, Histomonas,
E.coli and Mycotoxins using antibiotics will increase the
mortality rate


Must combine several drugs together in one disease

How to?
? www.ayurvet.com

Prevent and treat birds without Synthetic Antibiotics

Faster Recovery after Treatment

Reduce Mortality and kill only harmful micro-organism

Maintain Body weight of chickens during Diarrhoea

treatment

We need to change Method

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Antidiarrheal and Gut Function Modulator

Scientific Validations
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Poland Vietnam Philippines Hungary Thailand

Publications 13

Trial Reports 9

Thesis 3

 Herbal Active Ingredient

For Keeping Gut Integrity www.ayurvet.com

Berberis aristata Plantago ovata

Antidiarrhoeal, Gut function
Antibacterial modulator

These Optimal Combination of herbs and

special supplements helps to shorten

Aegle marmelos
treatment time
Acacia catechu
Punica granatum
Antidiarrhoeal, Astringent, Antiviral,

Tonic, Antimicrobial Antioxidant, Antimicrobial, Anti-
and Antidiarrheal amoebic and
Immunomodulatory antidiarrheal


And Many More

 Herbal Antimicrobial Effect

Salcochek pro www.ayurvet.com

The hydrophobic character of EOs enables them to partition in the lipids of the cell membrane and
mitochondria, rendering them permeable and leading to leakage of cell contents

Disintegrate the outer membrane of Gram-negative bacteria releasing lipopolysaccharides (Burt, 2004)

Mechanism of Action Salcocheck Pro

Salcocheck Pro
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Mechanism of Action Salcocheck Pro
Salcocheck Pro www.ayurvet.com

Healthy Intestinal Tract

Mechanism of Action Salcocheck Pro
Salcocheck Pro www.ayurvet.com

Before

Shorter villi
Salcochek

Longer villi

After

 Mechanism
No effect by pH value in the intestine www.ayurvet.com
pH

Salcochek
Salcochek Way to Multiply Profits

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Kill Harmful microorganism only: E.coli, Salmonella spp, Clostridium perfringens, Balantidium
coli

Recover Villus, reduce inflammation, protect layer part and increase Goblet cell

Balance electrolyte in intestine

Harmful bacteria do not develop resistance with Salcochek
Salcochek
Increase digestibility and optimum feed intake, increase FCR

Reduce Smelly feces through that and reduce respiratory syndrome

and protect environment

Validation at Greece

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Effect of New Gen Phytogenic in

Broiler

Performance
Gut health
Gut Microbiota
Digestibility
Immunity
Antioxidant Status
Carcass Traits
SALCOCH
EK
Experimental Design
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DOC Ross 308 2 groups 8 Subgroup

DOC Ross - 308 - 2 8

640 DOC

128 DOC 128 DOC

CONTROL SALCOCHEK
Basal Diet 500 g

EQUAL MANAGEMENTAL CONDITION, FEED & WATER FREE FLOW

Results

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PERFORMANCE

PERFORMANCE

CONTROL Improvement
SALCOCHEK

Body Wt. 2.535 Kg 62 g / Bird
2.473 Kg

FCR Better
1.78 1.72

Better Performance with Food Safety Guaranteed !!

Results

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Digestibility of Nutrients

DIGESTIBILITY NUTRIENT

CONTROL Improvement
SALCOCHEK

Dry Matter
70.3 70.7 ..
Crude Protein
61.9 63.5 ..
Ether Extract
82.9 91.2 ..

More Retention of Nutrient and Less Pollution

Guaranteed !!

Results

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Intestinal Morphology
INTESTINE MORPHOLOGY

CONTROL SALCOCHEK Improvement
%
Villous Height
1357 1545 14 %
Crypt Depth
138 144 5%
Villi / Crypt
9.81 10.73 ..
Goblet Cells
185 202 9%

Villi Height - All measures in Micro Meter (um)

Better Assimilation & Absorption

Guaranteed

Results

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Phagocytosis and Immunity
IMMUNITY

CONTROL
CONTROL SALCO Improvement

CHEK %

Phagocytosis
NDV TITRE 1.98
1.98
1.98 2.05 ..
Heterophils

124
124
124 15 %
Intensity 142

Monocytes 168
168 182 9%
Intensity
Phagocytosis - Mean
NDV Ab Titer 1 (log 10)
Fluorescence Intensity

Better Protection and Immunity Guaranteed !!

Results

Antioxidant Activity www.ayurvet.com

ANTIOXIDANT
CONTROL Improvement
SALCOCHEK
Phenolic %
Content 1.9 34.18 ..

mg /g of dry matter
/

Better Free Radical Protection Guaranteed !!

Results

Pathogen Load www.ayurvet.com

GUT MICROFLORA
CONTROL SALCOC Improvement
HEK %
E.coli
83 X 105 12 X 105 85 / 51

Caecum
Clostridium P
41 X 104 2 X 103 950

Lactobacillus 15 X 105 17 X 105 13 / 260

Less Pathogenic, More Good Bacteria &

Reduction in Antibiotic usage Guaranteed !!

 Results

Fatty Acid Profile www.ayurvet.com

Control Salcochek
Breast Meat %

Saturated FA
32.160 29.630

Monounsaturated FA
32.421 35.544
Polyunsaturated FA
31.692 31.003

Control Salcochek
Saturated FA
Leg Meat %

28.476 33.418
Monounsaturated FA

38.419 36.328
Polyunsaturated FA
30.229 26.194

No significant variation observed

Results

Numbers of Eimeria Spp. Of chickens in response to diet and age www.ayurvet.com

Groups 21 d of age 42 d of age

21 42
Control 24 6

Salcocheck 30 6
Unit=103/gram of feces

No significant change in the Eimeria infection is seen

Results

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Sensory Evaluation of Cooked Meat Panel Test
-

Groups Aroma Taste Juiciness

Control 6 7 6

Salcocheck 7 7 7

Aroma , Taste and Juiciness enhances in Salcoheck Group

Salcoheckz
Conclusion

SALCOCHEK PRO www.ayurvet.com
Salcochek Pro has positive relations with body weight and FCR
Salcochek Pro
It improves gut health and microflora conditions

Better digestibility and gut fermentation characteristics

Enhanced immunity and antioxidant activity of the birds

Its administration leads to better carcass characteristics

Reduced antibiotic uses

Alternative to antibiotics and other chemicals

Cost effective

No residual effect

Mr. Giao
Vietnamese Progressive Farm
Total number 2000 laying hens
2000
Time to try experiment
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Before treatment After treatment
5 days use Salochek 2kg/ton of
feed and superliv 1ml/1litter
water
5salochek 2 superliv 1

Mortality There are two hens death/day No mortality

average

Laying rate 75% 76,5%

Water in feces Feces wet and contain a lot of Dry feces and less smelly
water

Smell of house cause by feces Very smelly Less smelly to much

 Mr. Thong Hai Duong Province
This farm had infected Histomonas disease average 10 Quails dead per day, Loss of egg color www.ayurvet.com
Histomonas10

Total number 5000 Quails 4 day use Sulfur mono + Amox colistin 4 days use Sulfamono + Salcochek
5000 4+ 4+Salcochek

Time to try experiment 4 days 4 days use Salcochek 2kg/ton of

4 feed and superliv 1ml/1litter water
4salochek 2 superliv 1

Mortality 10 quails dead per day, loss ( total Reduce 1 quail per day and go to 2 day
10 no mortality
12

Laying rate 55% 85%

Egg color 70 80 % loss Color and 30% Thin egg 95% recover and Egg shell become to
shell normal
70 - 8030 95
Smell of house cause by feces Very smell Less smell to much

Mr. Hi Thanh Ha Province
Vietnam Progressive Farm
Total number 8000 Broilers 4000 chick use Amox Colistin 4000 Chicks use Salcochek and www.ayurvet.com
8000 4000Amox- Superliv and Stresroak
4000Salcochek
SuperlivStresroak
Time to try experiment 20 days 5 days use Salochek 2kg/ton of
Use Amox Colistin 2kg/ton of feed feed and superliv 1ml/1litter water
Sorbitol 5Salochek 2kg /
20 Amox - superliv 1ml / 1L
2kg /
Mortality 50 Chicks had dead 7 chicks had dead
50 7
Sign of Salmonella 6% 1%

Water in feces Feces wet and contain a lot of Dry feces and less smell
water

Smell of house cause by feces Very smell Less smell to much

Change the Medicine / Feed Additive and change the way you
use
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/
Change the methodDo not Forget Change the way you use
Medicine...
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Find out main reason to handle and control them

1

- Prevention: Use Salcochek pro frequently in animal feed

Salcochek Pro
2 - Treatment: Use Salcochek pro two time/day to three time/day
Salcochek pro//

Use right dosage and adequate dosage

3
Salcochek Pro Powerful in Control of Salmonella spp and other
harmful bacteria in the intestine www.ayurvet.com

Prevent and treatment all sign off diarrhea like: Green feces, white feces,
feces with gas, wet feces. Cause by E.coli, Salmonella spp, Clostridium
perfringen , Balantidium coli

Control Diarrhea very fast compared with antibiotic

No affect by pH Value
pH

No resistance drug and no residual in meat, egg and milk

Use it Right and you will achieve more success!!!
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But one important thing we almost forget

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When liver had damaged: We Can not use antibiotic, it will infect
secondary bacteria, disease will be more complicated

Can not use Antibiotic with liver tonic

Liver tonic today can not recover each part of damaged liver

We will still fail and can not achieve success if we forget this www.ayurvet.com

So

How to recover liver

How to use liver tonic together with antibiotic and other medicine
use in raising poultry

 Some Factor effecting liver and kidney frequently

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.

Antibiotics as Diseases

prevention and
treatment of Newcastle,
Gumboro, CRD, ORT
Disease

High Protein and Toxin and Stress Factors

Fat Usage Mycotoxins

Vaccination,
More stress on Liver From bacteria, virus Transport, Neb
and excretion and feed molds Cutting
system

Neb
How to neutralize toxin and recover damage parts in liver?

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Liver damaged: Use too much Liver damaged by : Salmonella spp;

Antibiotic Histomonas

This is Salmonella Spp

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Broiler, Breeder and Layer

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Fatty Liver Infection Kidney Urate Deposition

Poor Digestion of the intestinal Tract due to decreased secretion
of bile
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Liver tonic Drug when inside in the gut and mechanism

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Can not combine with antibiotic and other drug

Can not increase ability regret spleen liquid => reduce digestibility
=>

antibiotic
Liver antibiotic

tonic Drug

So what is the solution
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Most of liver tonic Drug only release

toxin and can not recover damage cell
of liver


Use singular only. Cannot mix or
combine with antibiotic and other Drug

Chicken infect disease lose body weight
and take long time to recover

Introducing SUPERLIV
SUPERLIV
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100% Herbal, without Residues in the body of birds
100 Hepato-Protective

Hepato-Regenerative

Hepato-stimulant

Di-uretic

Anti-hepatotoxic

Immuno-modulator

Superliv
Mechanism of Action of Superliv
Superliv
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Sup Sup
Sup

Sup Sup

Sup

Regeneration of Liver Injury Lesions Fatty Liver Recovery

Superliv can combine with any drug and increase potency
Superliv
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 One Experiment for Performance

From POLAND www.ayurvet.com

The experimental materials comprised 40 Ross 308 broiler chickens randomly divided into two
groups with five replicates of 4 birds each (2 females and 2 males). The experiment lasted for 42
days.
40Ross 30842542
Control and experimental groups broiler chickens were fed a complete diets. Experimental group II
birds received the Superliv in the drinking water during whole period of experiment.
Superliv
The body weights and feed intake of chickens were determined at one-week intervals. A slaughter
analysis was performed on day 42 of the experiment. Breast muscles, abdominal fat, hearts and
livers were separated and weighed.
42

Results

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Item Groups
I - K II (Superliv) SEM P

Duration of trial, d 42 42

Final body weight, g 58.94 0.09

2920.4 3121.7

FCR, kg/kg 2.06b 1.86A 0.04 0.008

Livability, % 95.0 95.0 3,33 1,0

EEI* 314.61 379.29 0.09 0.13

*European Efficiency Index

a, b - P0,05, A, B - P0,01
Post-mortem Liver
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Control group

Experimental group

Nutrient Trial

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A trial was conducted to see the efficacy of Superliv while varying

the Crude protein and Metabolize energy.
Superliv
Here is the depiction of reduction of 4% ME
4

Dr.C.Suresh,
Dept. of Animal Nutrition
Veterinary College and Research Institute
Email: c.suresh@tanuvas.org.in
Mobile: 09042408216

Department of Animal Nutrition

Veterinary College and research Institute
Tamilnadu Veterinary and Animal sciences
University
Supeliv Graded Energy Reduction
Supeliv
Body Weight www.ayurvet.com

X 00,000 VND 85 gm 38 gm
BODY WEIGHT
(85) gm 395 MYR 169 MYR
2250
(395 MYR 1000 Bird
Superliv 2213
2200
(34) gm 2272
(150) MYR 2162
2150 -8%
2128 Standard -4%
2115 0
2100
2077 -4%

2050 -8%

2000

-8% -4% 0 -4% -8%

SUPERLIV
Superliv Graded Feed Energy Reduction Experiment
Superliv
Feed Consumption www.ayurvet.com

X 00,000 VND

FEED CONSUMPTION
4700
4655
4600 39 gm 4617
4500 (560) gm 64 MYR 1000 Bird
Superliv
4400 (846) MYR 3998
38 gm -8%
4300 4321 Standard 4282 56 VND -4%
4095 0
4200
-4%
4100 (226) gm
-8%
(263) MYR
4000

3900

3800
-8% -4% 0 -4% -8%
SUPERLIV
Perfect Combination

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Superliv can mix with any antibiotic

Superliv
Superliv recover liver organ
Superliv
Superliv can use every day to optimum health and digestibility
Superliv
How to control Salmonella Spp, E.coli and Clostridium
perfringens

Sure Shot Proven Method www.ayurvet.com

Environment

Prevent and treatment

- Prevent: Use Salcochek pro 1.5 2kg/ton of feed, Superliv 1 2 ml/1liter water or 10ml/1kg food.
Use for 5 7 days
- Salcochek pro 1.5 2 /Superliv 1 - 2 / 110/ 15 7
Treatment: Salcochek pro 2 3kg/ton of feed or 1 kg/ 5 ton of body weight
+ Plus those product if necessary Superliv + probiotic + Electrolyte in water + Paracetamol ( in cases of
fever)
Salcochek pro 2 3 /1/ 5
Superliv +++
Superliv is Number One in Restoration and Anti-hepatoxic
effects www.ayurvet.com
Superliv
In complicated Cases

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Salmonella spp or E.coli or Clostridium perfringens has signed of high fever

and respiratory

Use Salcochek to control All symptom from intestine
Salcochek
- Use Superliv to protect Liver and immune system
Superliv
- Use Respzz to treat any problem from respiratory
Respzz
In Case Respzz product is not available in your country you should used
antibiotic like: Tylosin, Amoxicillin, Doxycycline, Florfenicol
/Respzz
Some Disease can incorporate Antibiotic with herbal Solution

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Histomonas disease: Salcochek pro + sulfamonomethoxine or sulfadimethoxine

- Paracetamol, Probiotic, Electrolyte, Superliv
Salcochek pro +
-Superliv
*CRD or CCRD: Salcochek pro + Enrofloxacin or Nofloxacin or Amoxicilin..
- Bromhexine, Paracetamol, Probiotic, Superliv
: Salcochek pro +
- Superliv
*Necrotic enteritis: Salcochek pro + probiotic + Electrolyte , Superliv
Salcochek pro ++, Superliv
Thank you for your attention

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Lets Give Better Life with Herbal Solution to Our Consumers

SUPERLIV SALCOCHEK
 Thank You

Please contact for further enquiry

Dr. Praful Kumar

pverma@ayurvet.com
Mob - +91-9013474749
Skype - spcarf