MCB 5505/BSC 4933

VIROLOGY
SAMPLE TEST 1 - BACTERIOPHAGE
R. H. REEVES

THE FIRST THIRTY QUESTIONS ARE WORTH TWO POINTS EACH. THERE IS ONLY
ONE CORRECT ANSWER FOR EACH QUESTION. PLEASE CIRCLE THE CORRECT
LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE QUESTION
NUMBER.

1. HE CRYSTALLIZED A PURIFIED PLANT VIRUS AND HELPED FUEL THE

HEATED DEBATE OVER WHETHER LIVING SYSTEMS COULD BE COMPLETELY
DESCRIBED IN PHYSICAL AND CHEMICAL TERMS.
A. PEYTON ROUS
B. WENDELL STANLEY
C. A. D. HERSHEY
D. D. CARLETON GAJDUSEK
E. JONAS SALK

2. MOST ENVELOPED VIRUSES

A. CONTAIN BOTH RNA AND DNA
B. CONTAIN HOST MEMBRANE PROTEINS
C. ARE INSENSITIVE TO ORGANIC SOLVENTS
D. CONTAIN VIRAL GLYCOPROTEINS
E. ALL OF THE ABOVE

3. CIRCULAR DNA IS FOUND IN THE VIRIONS OF

A. BACTERIOPHAGE LAMBDA
B. T7
C φX-174
D. THE LEVIVIRIDAE
E. T2

4. AN ICOSAHEDRAL VIRUS WITH A TRIANGULATION NUMBER OF SEVEN HAS

___ HEXON CAPSOMERS
A. 30
B. 40
C. 50
D. 60
E. 70

5. THE GENOMIC DNA OF BACTERIOPHAGE LAMBDA

A. IS DOUBLE STRANDED
B. HAS STICKY ENDS
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE

6. BACTERIOPHAGE T7
A. MAKES ITS OWN RNA POLYMERASE
B. MAKES ITS OWN DNA POLYMERASE
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
KEY FOR QUESTIONS 7-16 THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE.

A. T2
B. MS2
C. M13
D. Qβ
E. T7
F. LAMBDA
G. TOBACCO MOSAIC VIRUS
H. φX-174

_____07 ITS DNA HAS cos AND att SITES

_____08. A HELICAL VIRUS WITH A SINGLE-STRANDED DNA GENOME

_____09. ITS GENES ARE EXPRESSED IN THE ORDER THEY APPEAR ON THE
GENOME

_____10. ITS LYSIS PROTEIN GENE OVERLAPS BOTH ITS COAT AND
REPLICASE GENES

_____11. MAKES AN "IMMUNITY SUBSTANCE"

_____12. A PODOVIRUS

_____13. ITS FOURTH GENE IS MADE BY A “READ THROUGH” OF A UGA

STOP CODON

_____14. VIRION HAS A CONTRACTILE TAIL

_____15. MEMBER OF THE SIPHOVIRIDAE

_____16. MAKES ITS OWN DNA-DEPENDENT RNA POLYMERASE

22. EXPLAIN HOW THE TRANSLATION OF THE COAT PROTEIN AND THE COAT
PROTEIN ITSELF REGULATE THE PRODUCTION OF THE RNA REPLICASE
IN PHAGE MS2.

23. HOW DOES THE CII GENE PRODUCT REGULATE THE PRODUCTION OF THE
LAMBDA REPRESSOR AND THE INTEGRASE?

24. BOTH LAMBDA AND φX-174 REPLICATE THEIR DNA BY THE ROLLING
CIRCLE MODEL OF DNA REPLICATION; HOWEVER, THE END RESULT OF
THIS REPLICATION IS VERY DIFFERENT. ONE PHAGE HAS UNIQUE, DS
DNA IN THE VIRION AND THE OTHER HAS A VIRION GENOME WHICH IS
A SS CIRCLE. EXPLAIN. (PLEASE USE DIAGRAMS)

25. WHAT IS THE FUNCTION OF THE TERMINAL REPEATS ON THE ENDS OF

T7 DNA?