91494734

Varian, Inc.
2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA
ProStar 520 Column Oven

Control
Galaxie Driver Manual
Varian, Inc. 2005 03-914947-34:Rev.2

Table of Contents
Introduction.............................................................................................. 2
General ........................................................................................................................... 2
Installation and Configuration................................................................ 3
Installing the Driver Software .......................................................................................... 3
Configuring the ProStar 520 Column Oven Communications ........................................ 3
Ethernet Communications ........................................................................................... 3
Configuring the Driver ..................................................................................................... 5
Configuring the Column Oven ProStar 520................................................................. 5
Configuring the Synopsis .......................................................................................... 10
Building a Method ................................................................................. 13
Creating a New Method ................................................................................................ 13
Control Method Section for the ProStar 520 Column Oven.......................................... 16
Oven Control ............................................................................................................. 18
Miscellaneous Control ............................................................................................... 20
Starting and Viewing an Acquisition.................................................... 21
Quick Start and Sequence ............................................................................................ 21
Quick Start (Single Acquisition)................................................................................. 21
Sequence .................................................................................................................. 23
Running Acquisition Status ........................................................................................... 24
Viewing a Running Acquisition .................................................................................. 24
Status of the ProStar 520 Column Oven................................................................... 24
ProStar 520 Column Oven Control Manual -1- 03-914947-34:2

Introduction
General
This document describes the configuration of the Varian ProStar
520 driver. It also explains how to build a control method for this
driver, how to start and view a running acquisition and what
actions are available during an analysis.
The ProStar 520 Column Oven controls the temperature of the

mobile phase and column for HPLC separation on a Helix
system. The column oven controls the temperature of all the
components inserted in the oven (autosampler injector, pre-
saturator column, analytical column, etc.).
By keeping all of these components at the same precise

temperature, more reproducible chromatography is obtained.
The oven has a vapor sensor to detect any organic solvents in

the oven. It will automatically shut down the oven heater if there
is a leak from any of the tubing.

Installation and
Configuration
Installing the Driver Software
To install the Galaxie Drivers software refer to the Galaxie

installation Guide.
Configuring the ProStar 520 Column Oven

Communications
Ethernet Communications
The ProStar 520 Column Oven uses Ethernet as the method of
communication for instrument control.
All signals are connected through the rear panel of the oven.
Only the J10 connector is used to connect the oven to the
Galaxie Workstation computer. The other connections are not
used.
The column oven is connected using either an Ethernet

crossover cable or a standard Ethernet cable.

J2 SYNC Cable Connector
J4 S.I.D. Cable Connector
J7 A/S Power
J 10 COMM Connector
RJ45 connector for 10 baste T cable for
Ethernet communications
J 12 SERVICE Connector
F1 Fuse
User accessible fuse designed to
protect the 24 volt dc supply.
AC Power Input
Standard power cable connector
Connectors for Peripheral Equipment
The ProStar 520 uses TCP/IP (Transmission Control Protocol /

Internet Protocol) to communicate over the Ethernet network and
needs to be given a unique IP address to identify the instrument.
A BOOTP Server is used to send IP addresses to TCP/IP

devices.
The BOOTP Server lists Ethernet addresses along with the IP

addresses that are to be assigned to the corresponding devices.
To known how to configure the BOOTP server, refer to the

Galaxie installation Guide.
NOTE: The BOOTP server provided by the Galaxie Chromatography Data System is
installed during the installation of the Galaxie software.

Configuring the Driver
The driver software configuration is done in the Galaxie
Configuration Manager when a new chromatographic system is
created.
Configuring the Column Oven ProStar 520
Click on the button corresponding to the Varian ProStar

520 driver and the configuration screen appears.
Four sub-sections allow you to configure the instrument:

Communication, Column Oven, Vapor Sensor, and
Miscellaneous.
The toolbar allows you to easily scroll through the configuration

windows:
to select the first configuration window.
to select the previous the configuration window.
to select the next configuration window.
to select the final configuration window.
to send the configuration to the instrument.
to get the configuration from the instrument.

Communication Window
Enter the IP address of the ProStar 520 Column Oven (declared

in the BOOTP Server) in the GC name or address field or click
on the button to find the instrument previously configured
with the BOOTP Server program and select it.

Press the button to automatically
get the configuration from the instrument.
Column Oven Window
The temperature of the oven can be calibrated to 0.1C in the

lab. Calibrating the temperature has three basic uses:
If you want to adjust the temperature to the exactly
correct temperature, you can calibrate the oven
using a high accuracy temperature measuring
device.
If you want to adjust the temperature set in and read

from the oven based on results for a particular set of
compounds published in the scientific literature.
If you want to adjust the temperature of many

different ovens in the same lab to the same
temperature.

To calibrate the oven, use the following steps:
1. Run one or more standards with setting the temperature to

several values in the range where the separation should
occur (this can be in 0.1 or 0.2 C increments).
2. By looking at the chromatograms, decide what the actual

temperature should be for a particular separation.
3. Select the Column Oven sub-section and choose the

Constant Correction option from the list, if you want to
perform the calibration at one point or select the Linear
Correction option, if you want to perform the calibration at
two points.
4. Enter the temperature that you set in the column oven under
the ProStar 520 heading (Temperature data point 1 or 2
entry).
5. Enter the temperature at which this separation should occur

in the Calibration heading (Temperature data point 1 or 2
entry).
If you are going to run only one set of samples at one

temperature, use the single point calibration (Constant
Correction calibration mode). If you are going to run sample at
several temperatures, you can use a two point calibration. For
this, run two different standards at the two bounds of the
temperature range of interest.
The calibration model is a linear curve with the equation:
Calibrated Temperature = (Oven Temperature x a) + b
Where the coefficients, a and b, are derived from two point

calibration; if a one point calibration was done, a is equal to 1
and b is the only coefficient used.

Vapor Sensor Window
The vapor sensor is located in the column oven. It is designed to

detect leaks of organic chemicals such as acetonitrile in the
mobile phase in the column oven. If it detects a leak, it will
automatically turn off the column heaters, open the column oven
vent, and keep the vent fan running. This will help to clear the
oven of any solvent.
The vapor sensor only responds to organic chemicals. It does

not respond to water or buffer. If there is a water only leak in the
column oven, no alarm will be triggered.
The vapor alarm can trigger three actions besides shutting down
the oven. It can shut down the pumps, turn off the lamps and
sound an audible alarm in the PC which displays the status of
the ProStar 520 Column Oven. Check the required options in the
Vapor Alarm Actions fields.

Miscellaneous Window
The Ready in Contact Closed option allows you to specify what

switch state corresponds to external device Ready. Check the
option if the closed state means Ready.
Configuring the Synopsis
In the Overview part of the system configuration, a synopsis can

be defined for the system. This synopsis depends on the
configured devices.
1. To save the configuration of these devices, click on the OK

button.
2. Right click on the system name in the bottom part of the

Galaxie Configuration Manager screen. A pop-up menu
appears: click on Stop. Then right click on the system name
again, and click on Start.
ProStar 520 Column Oven Control Manual - 10 - 03-914947-34:2

3. Go back to the System Configuration screen (Properties
wizard). A graphic of the devices that comprise the system is
displayed in the synopsis screen. To configure the synopsis
as the real instrument configuration, move each module,
then connect it appropriately. To do this, select one anchor
for each of the two modules (the selected anchors become
red) and click on the button ; a connection tube will

appear between the two anchors.
Click on the button to delete a selected tube, click on
the button to delete all the tubes.
Click on to define a background color, or on to

make the background transparent.
Click on the icon to change the grid color.
4. Once the synopsis configuration is complete, it will be

displayed in the Galaxie Chromatography Data System, in
the Systems tab. In this tab, the Overview will show the
synopsis of the system with some parameters displayed
(temperature, flow, signal, etc.) depending on the installed
devices.
For example, the following pictures represent the synopsis of

the ProStar 520 Column oven:
Galaxie Configuration Manager Screen

Galaxie Chromatography Data System screen
NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start acquisitions. For more
details about the creation and configuration of a system, refer to the Galaxie
Configuration Manager Users Guide.
Click on the button to print the current configuration.

Building a Method
Creating a New Method

The method contains parameters for instrument control, data
acquisition, chromatogram processing, and editing of final
results. To initially set up a method, it is not necessary to define
all of the sub-method sections, however, you must fill in the
instrument control section prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD

option from the Galaxie Chromatography Data System main
menu.
A wizard guide will appear and provide assistance during the

first steps of method creation:

2. Choose the system (chromatograph) associated with the
method. A method is created for a particular system. When
starting an acquisition, the name of the system that performs
the acquisition must be correct, since method access is
limited only to those methods associated with this system.
3. Once this field is completed, click on the Next button to

move to the second step of the method creation:

4. Enter the New Method Name in the first field. In the
Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the OK

button. The new method is created and is opened with
default parameters in the Galaxie Chromatography Data
System. The next step is the definition of each of the method
sections.
The control section must be defined in order to start an

acquisition. In the following part of this guide, only the control
section for the ProStar 520 Column Oven is detailed. Other
parameters such as Injection Volume, Vial and Rack number
may be necessary and are defined in the acquisition section.
NOTE: For more details about the programming of each method section, turn to the
Galaxie Chromatography Data System Users Guide.

ProStar 520 Column Oven Control Method
Once the new method is created, select the control section in the
lower part of the browser.
The following screen appears:
Press the button to display the System Control Method

Advanced Tools window:
to send the current method to the instrument
to get the active method from the instrument

to print the current method
Click on the button to display the ProStar 520 Column

Oven control method section. It contains two sub-sections: Oven
and Miscellaneous. Click on the required sub-section to define
the corresponding parameters that will be used during the
chromatographic run.
The toolbar allows you to easily scroll through the control method
windows:
to select the first window of the control method.
to move back to the previous window of the control

method.
to move forward to the next window of the control

method.
to select the final window of the control method.

Oven Control
General Parameter Group:
Enter the Stabilization time in the corresponding field.
Column Oven Program Parameter Group:
Check (or uncheck) the Heater option to turn it ON (or OFF).
Use the table to build a temperature ramp program to heat

and/or cool the column oven.
The first row of the table contains the initial temperature and hold
time for the column oven. The column oven will equilibrate to this
setting and stabilize for the specified Stabilization time (defined
in the General field), when the method is activated, and will be
restored to this setting when each chromatographic run is

completed. The rest of the table contains the programmed
settings for each of the ramp segments. Enter the final
temperature of each ramp segment in the Step column, the Rate
(C/min) and the Time the column oven is held at this
temperature in the corresponding column.
Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.
The graph shows the column oven temperature program.
NOTE: The browser always displays the current end time of the column oven program.

Miscellaneous Control
Driver Behavior Parameter Group:
Check or uncheck the Reset oven program at the end of the

acquisition to activate or deactivate this option. If it is checked
and the acquisition is stopped from the acquisition window, the
oven temperature program will be reset before continuing with
the next injection (if a sequence is running).
Check or uncheck the Stop oven at the end of the acquisition to

activate or deactivate this option.

Starting and Viewing an
Acquisition
Quick Start and Sequence

Once the instrument control method has been correctly
programmed, an analysis can be started. The next section briefly
describes how to start an acquisition.
Quick Start (Single Acquisition)

1. Press the button.
In the Quick Start window, select the system, the method

and optionally the project if connected in "All project" mode.
2. Press the OK button.

The following screen is displayed:
In the Acquisition Parameters tab, required fields like the

Run Name, the Run ID and the Acquisition length must be
filled before starting an analysis. Furthermore, if the system
is equipped with an autosampler controlled by the Galaxie
Chromatography Data System, the Injection Volume, the Vial
position and the Rack number (if required) must be entered.
Other parameters associated with each acquisition channel

can be entered in the Channel X tab.
3. Click on the Start button to start the acquisition if the system

is a single-injector system or click on the Next button if the
system is a multiple injector system. In a multiple injector
system the same parameters entered for the first injector
must be entered for the other injectors before starting the
acquisition.

Sequence
1. To create a sequence, select FILE / NEW SEQUENCE
menu, and complete the displayed creation wizard screen
(refer to the Galaxie Chromatography Data System Users
Guide), by entering the system name that will perform the
analysis, the number of lines (corresponding to the number
of samples), and the sequence name.
2. The sequence is displayed. Complete the sequence

information columns (refer to the Galaxie Chromatography
Data System Users Guide).
The Method Name, the Run Name, the Run ID, and the
Acquisition length must be filled before starting the analysis.
All these parameters are automatically imported from the
acquisition part if defined in the method.
3. Start the sequence by clicking on the button.
NOTE: For more details about how to start an acquisition, refer to the Galaxie
Chromatography Data System Users Guide.

Running Acquisition Status
Viewing a Running Acquisition

To view a running acquisition, click on the Systems tab then
select the running system. The running chromatogram and the
status window where the driver modules are listed.
ProStar 520 Column Oven Status
Click on the button corresponding to the ProStar 520

Column Oven driver and the ProStar 520 status screen will
appear. It contains four sub-sections: Oven, Vapor Sensor,
Miscellaneous, and Overview. Click on the required sub-section
to display the corresponding parameters that will be used during
the chromatographic run.
A toolbar allows you to easily scroll through the status windows:
to select the first status window.
to move back to the previous status window.
to move forward to the next status window.
to select the final status window.

Oven Window
The status display includes the following parameters: :
Ready or Not Ready: the column oven is Ready if the setpoints

have been achieved and stabilized. The column oven is Not
Ready if readiness to start a run has not been achieved or if,
during a run, the oven becomes not ready or goes out of
tolerance.
Fault or No Fault: displays if a fault in the column oven

component has occurred.
Heater: displays if the column oven component is turned OFF or

ON at the ProStar 520.
Actual: displays the actual temperature of the column oven at the

ProStar 520.
Setpoint: displays the column oven programmed temperature

setting in the active method.
Stabilization State: displays the current stabilization state of the

column oven. When the state is ON, the
button becomes active. Click on it to bypass the stabilization
time.
Stabilization Time: shows the ProStar 520 Column Oven

stabilization time in minutes. The stabilization time counts down
from the programmed method value and then displays 0.00
minutes when the stabilization period is over.

Click on to turn off the oven when the oven
is on.
Click on to turn on the oven when the oven

is off.
Vapor Sensor Window
Ready or Not Ready: indicates whether the vapor sensor is OK

or not.
Fault or No Fault: Displays if a fault in the vapor sensor

component has occurred (when it has detected vapor in the
oven).
Click on to turn off the vapor sensor alarm.
NOTE: If a vapor sensor alarm or failure occurs, it can only be cleared by turning the
oven off and turning it back on. If the cause of the alarm was a spill in the oven,
the oven should be carefully cleaned of all spilled material and the vapors should
be allowed to dissipate. This is aided by the fan continuing to run when there is
an alarm. If the alarm turns on after it has been restarted, the procedure should
be repeated. Note, if the atmosphere in the laboratory is so full of vapor that the
alarm continues to go on, the instrument should be moved to a safer
environment.
If the vapor alarm has been triggered because of a failure of the vapor sensor,
the instrument should be turned off and back on.

Miscellaneous Window
The miscellaneous screen contains general information about

the current run and the overall state of the instrument.
The state of the instrument (Ready, Running, Stabilizing,
Equilibrating, or Not Ready) is displayed.
A Fault / No Fault indicator is also shown. When a hazardous or

disabling fault occurs, any run in progress will halt and the
affected component will shut down. If the fault is recoverable,
then the run is not terminated.
The run time bar displays the elapsed time in minutes since the
beginning of the run and the end time.
Line Voltage: displays the voltage setting for the ProStar 520
Column Oven.
Firmware Version: displays the version of the firmware installed

in the ProStar 520 Column Oven.
Press the button, to reset the column

oven. If it is RUNNING, the oven aborts the current run, goes to
the READY state and continues with the next injection in the
sequence.

Overview Window
Click on the button. The Status Overview window is

displayed and contains three tabs:
General: displays the synopsis of the instrument

where some current values can be read (oven
status, column oven temperature, etc.).
Not Ready: contains the not ready information sent

by the instrument.
Events: contains the events that have occurred on

the instrument.
TROUBLESHOOTING:
If the status is reporting that the vapor sensor is giving a fault when there is no
discernable liquid in the oven, check the message carefully. The vapor sensor will report
whether it has failed or whether it can no longer read vapor.
If the vapor sensor reports vapor in the oven when there is no liquid, it is possible that a
previous leak in the oven has saturated the insulation. If that is the case, continue to run
the oven with the fan on (the heaters will be off) and wait until the fault disappears. If the
fault does not go away within a reasonable length of time (1 day), it is possible that the
atmosphere in the lab has a too high saturation of organic vapor. If this is the case, move
the system to an area where the concentration of organic vapor is known to be low and
run it again.

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Varian, Inc.

2700 Mitchell Drive

ProStar 520 Column Oven

Galaxie Driver Manual

Varian, Inc. 2005 03-914947-34:Rev.2

ProStar 520 Column Oven Control Manual -1- 03-914947-34:2

The ProStar 520 Column Oven controls the temperature of the

By keeping all of these components at the same precise

The oven has a vapor sensor to detect any organic solvents in

ProStar 520 Column Oven Control Manual -2- 03-914947-34:2

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie

Configuring the ProStar 520 Column Oven

The column oven is connected using either an Ethernet

ProStar 520 Column Oven Control Manual -3- 03-914947-34:2

J4 S.I.D. Cable Connector

Connectors for Peripheral Equipment

The ProStar 520 uses TCP/IP (Transmission Control Protocol /

A BOOTP Server is used to send IP addresses to TCP/IP

The BOOTP Server lists Ethernet addresses along with the IP

To known how to configure the BOOTP server, refer to the

ProStar 520 Column Oven Control Manual -4- 03-914947-34:2

Configuring the Column Oven ProStar 520

Click on the button corresponding to the Varian ProStar

Four sub-sections allow you to configure the instrument:

The toolbar allows you to easily scroll through the configuration

to select the first configuration window.

to select the previous the configuration window.

to select the next configuration window.

to select the final configuration window.

to send the configuration to the instrument.

to get the configuration from the instrument.

ProStar 520 Column Oven Control Manual -5- 03-914947-34:2

Enter the IP address of the ProStar 520 Column Oven (declared

ProStar 520 Column Oven Control Manual -6- 03-914947-34:2

Column Oven Window

The temperature of the oven can be calibrated to 0.1C in the

If you want to adjust the temperature set in and read

If you want to adjust the temperature of many

ProStar 520 Column Oven Control Manual -7- 03-914947-34:2

1. Run one or more standards with setting the temperature to

2. By looking at the chromatograms, decide what the actual

3. Select the Column Oven sub-section and choose the

5. Enter the temperature at which this separation should occur

If you are going to run only one set of samples at one

The calibration model is a linear curve with the equation:

Calibrated Temperature = (Oven Temperature x a) + b

Where the coefficients, a and b, are derived from two point

ProStar 520 Column Oven Control Manual -8- 03-914947-34:2

The vapor sensor is located in the column oven. It is designed to

The vapor sensor only responds to organic chemicals. It does

ProStar 520 Column Oven Control Manual -9- 03-914947-34:2

The Ready in Contact Closed option allows you to specify what

Configuring the Synopsis

In the Overview part of the system configuration, a synopsis can

1. To save the configuration of these devices, click on the OK

2. Right click on the system name in the bottom part of the

ProStar 520 Column Oven Control Manual - 10 - 03-914947-34:2

red) and click on the button ; a connection tube will

Click on the button to delete a selected tube, click on

the button to delete all the tubes.

Click on to define a background color, or on to

4. Once the synopsis configuration is complete, it will be

For example, the following pictures represent the synopsis of