Arabidopsis Genetics Post-Lab Assignment

(10 points)

Name:_____Jeff Tong________ Lab Section___105______

Table 1: Phenotypic Analysis of Arabidopsis seedlings

Seedlin Trichom Hypocot Seedlin Trichom Hypocot Seedlin Trichom Hypocot
g es yl g es yl g es yl
No. ( + or - ) Length No. ( + or - ) Length No. ( + or - ) Length
(S, M or (S, M or (S, M or
T) T) T)

1 + M 21 + S 41 - M

2 + M 22 42 - S

3 + S 23 - S 43 - S

4 + M 24 44 - S

5 + M 25 45 + L

6 - S 26 + M 46 + M

7 + M 27 + L 47 + M

8 28 + L 48 - M

9 + S 29 + S 49 + S

10 + S 30 - L 50 - M

11 - M 31 + L 51 + S

12 32 + L 52 - M

13 - S 33 + S 53 - S

14 + S 34 + M 54 + S

15 + S 35 + M 55

16 36 - S 56

17 37 - M 57
 18 + S 38 58

19 + L 39 59

20 + S 40 + M 60

Table 2: X2 Analysis of Phenotypic Analysis of Arabidopsis seedlings

sample of ___45__ seedlings

+ - short medium tall

trichomes trichome hypocotyls hypocotyls hypocotyls
s

observed (o) 30 15 21 17 7
expected (e) 34 11 11 23 11
deviation (o- -4 4 10 -6 -4
e)

(o-e)2 16 16 100 36 16

(o-e)2/e 0.4706 1.4545 9.0909 1.5652 1.4545

X2=((o-e)2/ X2=1.9251 X2=12.1106

e)

Table 3: Hypothesis Testing of Phenotypic Analysis of Arabidopsis seedlings

Degrees of Critical Value Reject Null

Freedom Hypothesis?

trichome 1 3.84 No
character

hypocotyl 2 5.99 Yes

length
character
Table 4: Expected (Predicted) Phenotypic Ratios for the Dihybrid Cross

Phenotype Expected Ratio (show your math)

short hypocotyl + trichomes (3/16)*45 = 8

short hypocotyl - trichomes (1/16)*45 = 3

medium hypocotyl + trichomes (6/16)*45 = 17

medium hypocotyl - trichomes (2/16)*45 = 6

tall hypocotyl + trichomes (3/16)*45 = 8

tall hypocotyl - trichomes (1/16) *45 = 3

Table 5: X2 Analysis of the Dihybrid Cross

sample of ___45___ seedlings

short short medium medium tall tall

+ - + - + -
trichomes trichome trichomes trichomes trichomes trichomes
s

observed (o) 14 7 11 6 6 1
expected (e) 8 3 17 6 8 3
deviation 6 4 -6 0 -2 -2
(o-e)

(o-e)2 36 16 36 0 4 4

(o-e)2/e 4.5 5.3333 2.1176 0 0.5 1.3333

X2=((o-e)2/ X2=13.7842
e)

Table 6: Hypothesis Testing of Dihybrid Cross

 Degrees of Critical Value Reject Null
Freedom Hypothesis?

Sum of all 5 11.07 Yes

characters

Part II Questions (page 5-25):

14. Used the pooled data from the Canvas site for the following calculations:
a. What percentage of the plants with a CC genotype had the expected
phenotype?

54.17%

b. What percentage of the plants with a Cc genotype had the expected

phenotype?

45.45%
c. What percentage of the plants with a cc genotype had the expected
phenotype?

68.42%

15. Why do you think the percentages that you calculated in #14 above are less
than 100%?

Due to the subjectivity of classifying a hypocotyl as short, medium, or tall, the

results will vary depending on who is classifying, and even what angle it was
viewed at (some plants grew horizontally or diagonally, which may have had an
effect on their perceived or actual length). In addition, as similarly seen in human
height, length of a hypocotyl may depend on environmental factors, and beyond
this, length may be represented on a continuous spectrum instead of purely
segregated into three distinct categories of short medium and tall.