Media Uses

Alkaline Peptone Water Enrichment media for Vibrio cholerae

Alkaline Salt Transport
Medium
Transport media for diarrheal diseases
suspected of being caused by V. cholerae
Taurocholate Peptone
Transport Medium
Liquid media by addition of

Glucose (0.5 % to 1 %)
Ascorbic Acid (0.1 %)
Anaerobic Media
Cysteine (0.1 %)
Sodium Merceptoacetate (0.1 %)
Thioglycollate (0.1 %)
Particles of cooked meat broth
Bile Salt Agar Selective media for V. cholerae
 Thiosulphate Citrate Bile
Salts-Sucrose Agar (TCBS)
Monsurs Tellurite
Taurocholate Gelatin Agar
Bile Esculin Agar Selective media for Enterococcus species
(Contains 40% Bile) (Black coloration of the medium)
Enriched media (Supports the growth of
fastidious organisms, e.g. Streptococcus)

Indicator media to show hemolytic

Blood Agar properties of certain organisms
(Staphylococcus aureus: -Hemolytic;
Streptococcus pneumonia and S viridans:
-Hemolytic; Enterococcus: Non-Hemolytic)

Bordet-Gengou Agar

Charcoal Blood Agar

Regan-Lowe Medium Isolation of Bordetella pertussis
(Charcoal Agar with blood,
cephalexin and
Amphotericin B)
Brain Heart Infusion Used in blood culture bottles (both adult
Broth and pediatric patients)
Buffered Charcoal Yeast
Specialized media for isolation of
Agar (BCYA)
Legionella
 Feeley Gorman Agar
Campylobacter Selective holding media for recovery of
Thioglycollate Broth Campylobacter species
Biphasic medium for the isolation of
Castaneda Medium
Brucella
Cefoxitin Cycloserine
Selective media for isolation of Clostridium
Fructose Agar (CCFA)
difficile form suspected cases of
pseudomembranous colitis/antibiotic-
Cefoxitin Cycloserine
associated diaeehea
Egg-Yolk Agar (CCEY)
Cefsulodin-Irgasan-
Selective media for Yersinia (and may be
Novobiocin medium (CIN
used for Aeromonas also)
Medium)
Columbia-Colistin
Selective media for the isolation of Gram-
Nalidixic Acid agar (CNA
positive cocci
Agar)
Cooked meat broth

Nutrient agar slopes

In general, used for preservation and
Semisolid nutrient agar
storage of bacterial cultures
stabs
Heated blood agar
slopes
Selective media for Streptococcus
Crystal violet blood agar
pyogenes
Cysteine Lactose
Most commonly used media for culturing
Electrolyte Decient Media
urine samples
(CLED Media)
 Preservation of cultures of Gram-negative
Egg Saline Medium
bacilli
Detection of lipase and lecithinase activity
Egg Yolk Agar
of Clostridium species
Ellners Medium

Medium of Duncan and Specialized media to induce sporulation in

Strong Clostridium
Medium of Phillips
Alkaline Egg Medium
Fildes Blood-Digest Agar
and Broth Enriched media for recovery of
Haemophilus inuenzae
Levinthals Agar
Firm Agar (4% to 6% Prevents swarming of Proteus mirabilis, P.
Agar) vulgaris and Clostridium tetani
Fletchers Agar

Ellinghausen and
McCullough Medium Solid media for isolation of Leptospira
Ellinghausen-
McCullough-Johnson-
Harris (EMJH) Media
Glycerol Saline Transport
Transport stool specimen for typhoid bacilli
Medium
Growth of fastidious organisms (E.g.
Heated Blood
Hemophilus inuenzae, Neisseria
Agar/Chocolate Agar
gonorrhoeae and S. pneumoniae
 Hoyles Tellurite Lysed
Blood Agar Selective media for isolation of
Corynebacterium from throat swabs
Tinsdale Medium
Stimulation of metachromatic granules in
Loefer Serum Slope
Corynebacterium diphtheriae
Lowenstein-Jensen
Selective media for isolation of
Medium
Mycobacterium tuberculosis from sputum
and other samples
Middlebrook Media
Differential media for Enterobacteriacaeae
MacConkey Agar (i.e., lactose fermenting and non-lactose
fermenting)
MacConkey Bile Salt
Lactose Agar

Brilliant MacConkey Agar

Leifsons Deoxycholate-
Citrate Agar (DCA) Differential and media for isolation of
Wilson and Blairs Salmonella and Shigella from stool
Brilliant Green Bismuth- specimens
Sulphite Agar (BBSA)
Taylors Xylose Lysine
Deoxycholate Agar (XLD)
Hektoen Enteric Agar
SalmonellaShigella Agar
Mannitol Salt Agar Selective and indicator media for S. aureus
Modied Barbour Specialized media for Borrelia burgdorferi
Stoenner Kelly medium
(BSK)
Modied Korthoffs
Liquid media for isolation of Leptospira
Medium
Modied New York City
Medium (contains colistin,
Selective media for Neisseria gonorrhoeae
lincomycin, trimethoprim,
amphotericin B)
Performing antimicrobial susceptibility for
Mueller-Hinton Agar
bacteria
Basal media in microbiology
Nutrient Agar (1% to 2%
Agar) Supports the growth of all non-fastidious
organisms
Cultivation of parasites (e.g.,
Non-Nutrient Agar
Acanthamoeba)
Basal media for preparation for
carbohydrate fermentation media

Peptone Water To ascertain whether a bacteria is

motile or non-motile
Basis for Indole test

Phenol-Red Egg Yolk Selective media for isolation of Bacillus

Polymyxin Agar cereus from food, feces, and vomitus
Preservation of S. pyogenes, pneumococci,
Pikes Media and Hemophilus inuenzae in nose and
throat swabs
 Selective media for isolation of Bacillus
Polymyxin B-lysozyme-
anthracis from soil and other medium
EDTA-Thallous Acetate
materials containing numerous spore
(PLET)
formers of other species
Polymyxin B, Neomycin, Selective media for S. pyogenes (or -
Fusidic Acid Media (PNF) hemolytic Streptococcus)
PPLO Medium (Contains Specialized media for Mycoplasma
Sterol) pneumoniae
Pre-Reduced
Commercially available media for
Anaerobically Sterilized
anaerobic organisms
(PRAS) Media
Proteose Peptone-Yeast Media for carrying out biochemical tests
Extraction Broth for anaerobes
Requirements of X and V
Isolation of Haemophilus inuenzae
Factors
Roswell Park Memorial
Cultivation of malarial parasites (i.e.,
Institute (RPMI) 1640
Plasmodium)
Medium
Growth of anaerobes (e.g., Clostridium)
Robertson Cooked Meat
Broth (RCMB) Maintaining stock cultures of anaerobic
organisms
Salt-Cooked Meat Broth
Enrichment media for isolation of S. aureus
(Cooked Meat Broth with
from heavily contaminated materials
10% NaCl)
Prevents convection current and allows the
Semisolid Agar (0.05% to
growth of anaerobic and micro-aerophilic
0.1% Agar)
organisms
 Skirrows Campylobacter
Medium (contains
polymixin B, trimethoprim,
vancomycin)

Preston Campylobacter
Medium (contains Selective media for Campylobacter jejuni
polymixin B, rifampicin,
trimethoprim)
Campy Blood Agar
CVA Medium (contains
cefoperazone, vancomycin,
amphotericin)
Cultivation of nonpathogenic treponemes
Smith-Noguchi Medium (e.g., Reiter strain of Treponema
phagedenis)
Isolation of verocytotoxin-producing
Sorbitol MacConkey
(enterohemorrhagic) E. coli of 0157 type (as
Agar
it fails to ferment D-sorbitol)
Stuart Transport Media
Maintaining the viability of gonococci on
swabs during transportation
Amies Transport Media
Tetrathionate Broth
Enrichment media for isolation of Shigella
Gram-Negative Broth and Salmonella from stool samples
Selenite-F Broth
Thayer-Martin Medium
Selective media for Neisseria gonorrhoeae
(contains vancomycin,
colistin, nystatin)
Thioglycollate Broth All purpose enrichment broth for
anaerobes, aerobes, micro-aerophilic, and
Trypticase Soy Broth fastidious organisms
Selective and enrichment for
Todd Hewitt Broth with
Streptococccus agalactiae in female genital
Antibiotics
specimens
Triple Sugar Iron Agar Differentiation of various members of
(TSI) Medium Enterobacteriaceae
Performing antimicrobial susceptibility of
anaerobic bacteria
Wilkins-Chalgren Agar