Formal Report
Spectrophotometric Determination of the Acid
Date Performed: 6 Dissociation Constant of Methyl Red
November 2015
Date Submitted: 20 D.A. Mercado
November 2015
Department of Mining, The acid dissociation constant of methyl red was obtained by using the
Metallurgical and spectrophotometric method. The maximum wavelengths were determined from both the
Materials Engineering
College of Engineering acidic and basic forms of the methyl red and were used in obtaining the absorbance. In
order to determine the molar absorptivity constant, the sample solutions were prepared
Instructor: Searle by mixing the specified values of HCl and HMR as well as a mixture of NaOAc and
Aichelle Duay MR-. The acid dissociation constant was then determined by measuring the absorbance
of a mixture prepared by adding HOAc and NaOAc into the standard methyl red
solution. This then lead to the calculation of the acid dissociation constant at 1.3142 x
10-5 which had a percent error of 31.42% from the theoretical value of 1.00 x 10 -5. [1]
Table 1. Absorbances and Molarities of Solutions 1-6 AHMR ( HMR ,HMR )b(c HMR ) ( MR ,HMR )b(c MR )
Solution Absorbance Molarity [M]
HMR- [nm] MR- [nm] (6)
1 0.498 0.037 1.3962 x 10 AMR ( HMR ,MR )b(c HMR ) ( MR ,MR )b(c MR )
5
(7)
2 0.313 0.022 9.283 x 10 6
3 0.126 0.003 4.604 x 10 6 Equations (6) and (7) can then be further manipulated to
give equations (8) and (9) which then lead to the values
4 0.014 0.220 1.3962 x 10 calculated in Table 3.
5
Graph
Spectrophotometric Determination of the Acid Dissociation Constant of Methyl Red Mercado, 2015| 4
Figure 2. pH vs. log [MR-]/[HMR]
Calculations
Molarity of Solutions
(0.05 g )
Mstock = 0.00371333M
( 269.3 g / mol )(0.05 L)
Solution 1 and 4
2.5mL 5mL 15.04mL 5
0.0037133 M 1.3962 x10 M
50 mL 50mL 20 mL
Solution 2 and 5
2.5mL 5mL 10.00mL 6
0.0037133M 9.283x10 M
50mL 50mL 20mL
Solution 3 and 6
2.5mL 5mL 4.96mL 6
0.0037133M 4.604 x10 M
50mL 50mL 20mL
Solution 7
(0.872)(1282.33) (0.117 )(16670)
[HMR]= 1.264 x10 6 M
1[(3633.25)(1282.33) (16670)(39752)]
(0.117 ) (39752)(1)(1.264 x10 6 )
[MR ]= 5.2056 x10 5 M
(1)(1282.33)
Solution 8
(0.837)(1282.33) (0.181)(16670)
[HMR]= 2.954 x10 6 M
1[(3633.25)(1282.33) (16670)(39752)]
(0.181) (39752)(1)( 2.954 x10 6 )
[MR ]= 4.9575 x10 5 M
(1)(1282.33)
Solution 9
(0.785)(1282.33) (0.338)(16670)
[HMR]= 7.033x10 6 M
1[(3633.25)(1282.33) (16670)(39752)]
(0.338) (39752)(1)(7.033x10 6 )
[MR ]= 4.556 x10 5 M
(1)(1282.33)
Solution 10
(0.719)(1282.33) (0.549)(16670)
[HMR]= 1.2507 x10 5 M
1[(3633.25)(1282.33) (16670)(39752)]
(0.549) (39752)(1)(1.2507 x10 5 )
[MR ]= 4.0412 x10 5 M
(1)(1282.33)
Acid Dissociation Constant
10 4.881322016 1.3142 x10 5
Relative Error
(1.00 x10 5 ) (1.3142 x10 5 )
x100 31.42%
1.00 x10 5
Spectrophotometric Determination of the Acid Dissociation Constant of Methyl Red Mercado, 2015| 5