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Common Effects of Mefenamic Acid (C15H15NO2 ) in Liver of White Mouse (Mus musculus)

Aries Taliquig, RosemaeBerot,Jinky N. Francisco, July Mae Del Carmen, Chemmete Lamosnero,
Gerlyn Mae Daleon.

Agusan del Sur State College of Agriculture and Technology, Bunawan(8506), Agusan del Sur,
Philippines. Institute of Education, Department of Biology, Requirement for the completion of Course
Subject: Cell Biology

Abstract

(Rodents,Mefenamicacid,chronic kidney disease, Drugs and toxins)

1. Introduction
Non-steroidal anti-inflammatory drugs We received the experimental animal last
(NSAIDs) are widely- used therapeutic agents (1). More September 1, 2017 at Bunawan, Agusandel Sur (8506).
than 100 million NSAIDs are prescribed throughout the The Experimental animal was housed on 6x6
world, costing over US $5 billion (2). The analgesic cubic inch cell with food and water container inside
efficacy of mefenamic acid or Ponstan (2',3'- under a 12-hour light/dark cycle.
dimethylN-phenyl-anthranilic acid) has been
documented for inflammatory and non-inflammatory 2.3Experimental Design
pain such as pain after dental interventions or after
trauma. This experiment was focused to determine
Mefenamic acid is also used to treat whether there is a structural damage, which occurs in the
dysmenorrhoea. Despite its wide usage, it can cause Liver of white mice (mus musculus) as result of
serious toxicity such as severe gastrointestinal tract mefenamic acid severe intake.
disorders, hepatotxicity and nephrotoxicity(3). All experiments were conducted in Biology
Like other NSAIDs, mefenamic acid inhibits Laboratory 01 located in Agusan del Sur State College
prostaglandin biosynthesis, a fundamental element of of Agriculture and Technology Campus.
the analgesic, anti-pyretic and anti-inflammatory
properties of all drugs in this group(4). However, 2.4Preparation of Mefenamic acid
NSAIDs have recently gained attention as an effective
therapy for tumour patients (5). Prospective cohort Four bottles of 60 mL mefenamic acid syrup
studies indicated a protective effect of NSAIDs on the were obtained and preserved on a cooler >30 degrees.
incidence or mortality of colon cancer (6,7). However, Date of manufactured 11/2016, Expiration date 11/2019
the adverse drug reactions caused by NSAIDs limit the with batch number SLN143 ensuring its effective
usage of these drugs as anti-tumour therapy. We aimed formulation.
to evaluate the hepatotoxicity caused by mefenamic acid
in mice by focusing on the histopathological and 2.5 Treatments
biochemical changes.
Female white mice (25g, n=3) were identified as
"Rats and mice are mammals that share many
Treatment0 (-Control), Treatment2 (+Control),
processes with humans and are appropriate for use to
Treament2 (++Control) with a scrapped plywood as
answer many research questions," said J Haliski, a bedding. Mice were adapted to experimental conditions
representative for the National Institutes of Health (NIH) for seven days.
Office of Laboratory Animal Welfare. The mice received no treatment or were treated
daily with the drug (at 10ml mefenamic acid syrup/20ml
Animal models are being increasingly used in
of water + pellets & 20ml mefenamic acid syrup/10ml of
the study of disease progression and for safety
water + pellets for 7 days. Control animals received
assessments of new compounds. They are a powerful
equivalent amount of pellets.
tool for developing a more detailed understanding of the
The mouse in (t0=Negative Control) received
role specific genes play in biological pathways. At the
no treatment of mefenamic acid with pellets and free to
present time, the ability to introduce new genetic
tap water.
information into the germ line of complex organisms has
Control animals in (t1) & (t2) received unequal
completely changed and enhanced the study of all
amount of dosed (t1= at 10ml mefenamic acid
aspects of biologic processes.(Haliski)
syrup/20ml of water + pellets.) In (t2= the mouse
This research aim to determine whether if there
received 20ml mefenamic acid syrup/10ml of water +
is effect of Mefenamic acid severe intake into the liver
pellets) for 14 days.
structure and conduct Qualitative and Quantitative
observations if ever on structural damages it caused to
2.6 Dissection Method
the said body organs of white mouse (mus musculus),
basis for recommendations to human health cautions.
A mice dissection is a perfect way to show all
the organs in the body. You can analyze the body organ
system.
2. Materials and method
As we are dealing with organism the researchers
2.1Housing and Handling of the Animals make sure they clean their benches after use, we use
gloves and a lab coat. Take the appropriate safety steps
Three Healthy white mice (mus musculus)6 when dealing with the dissecting tools and dispose of the
months old were obtained through a generous donation mice appropriately.
of a private individual from Surigao City; further, we
considered its health, age and size for the visibility of 3. Results and Discussion
organs that are subject for observation.
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