4551/2
Chapter 4 : Chemical composition in the cell 2013
Carbohydrate
1
P1-It is a compound synthesized by plants through photosynthesis
1
P2-It is involved in the building of plant cell wall
1
P3-It is present in various forms including monosacharide, disaccharide and polysacharide
1
P4-Polycharide is storage carbohydrate
1
P5-Example: glycogen is storage carbohydrate
Protien
1
P1-It is the main component of cytoplasm an dplasma membrane
1
P2-protien is present as keratin in hair and nail
1
P3-Carier protein in the plasma membrane function in the transport of substances across
the plasma membrane 1
P4-Enzyme , antibody and many hormones are proteins 1
P5-Hormones function to regulate chemical process and cell metabolism , such as 1
digestion
P6-Enzyme act to catalyze metabolism reaction of the cell 1
P7-Antibody defends the body from pathogenic attack 1
P8-Protien also act as source of energy during starvation ANY 10 1 10
(b) How are the carbohydrates stored in plant and animal tissues?
Carbohydrate are stored as starch in glycogen and glycogen in animal tissue 1 1
(c) How are the delicate internal organs of animal protected by the lipids
Lipid form the adipose tissue lining around the delicate internal organsProtecting them
from physical injure 1 1
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Nucleic acid
Trial perlis 2010 Trail kedah 2012 JUJ 2009
Figure below shows the structure of nucleic acid
(d)
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(g) Diagram below shows an incomplete structure of DNA at one polynucleotide chain
complete the structure below
1
Drawing 1m
1 2
Nitrogenous bases matched correctly : 1m
(h) Segment of structure in diagram 2.1 carry characteristic for each individual .describe the
application of genetics that be used for
Identification purposes in solving criminal cases
F1-DNA fingerprint 1
P1-(it is used) to examine DNA from tissue sample of hair/ saliva/blood/semen found at 1 2
the scene of the crime then compare it to suspects DNA
4.2 Carbohydrate
1. Diagram 2 shows a series of reaction process is the formation of molecules Q while process Y is the
breakdown of polypeptide
Process X
Molecule Q
Process Y
Molecule P
Molecule R
Molecule X
(g) Explain how the complex molecule R are formed from their basic units
P1-Molecule R is polypeptide in which consist of amino acids 1
P2-The amino acids are joined together by condensation and arrange in a sequence to form a 1 2
long chain
(h) Explain the breakdown of molecule Y(Protein) Given basic units : amino acid
P1-Molecule is a polypeptide( which consist of many amino acids) 1
P2-Molecule Y is broken down by hydrolysis 1 2
2. Protein are large organic molecule which play diverse roles in living organism . Diagram 2.1 shows
four level of organization in protein structure JUJ 2009 Trail Kelantan 2008
Molecule P Molecule Q
(f) Amino acid is monomer of protein . There are two type o f amino acids which are essential
amino acid and non essential amino acid
Explain about essential amino acid
P1-canot synthesis by the body
P2-obtain from diet 2 2
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X Y Z
Molecule P
(b) State two important product obtained from the hydrolysis of lipid
Glycerol/fatty acid 1 1
Process Y: hydrolysis 1 2
(f) Name the other product of this reaction besides lipid molecule
Water 1 1
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(h) What is the essential structural difference between a phospholipids molecule and the lipid
molecule shown in the figure
P1-A phospholipids molecule has two fatty acids and phosphate group attached to a molecule 1
of glycerol
P2-ipid molecule in the figure has three fatty acids joined to a molecule of glycerol
1 2
(j)
Structure X
Molecule P is important to build the structure of membrane in the cell , including structure X
Name structure X
Plasma membrane 1 1
(k) Explain the arrangement of molecule P in X
P1-Molecule P consists of hydrophilic head and a hydrophobic tail 1
P2-the head part of faces the external surface of the membrane because it attract to water 1
P3-the tail part of P is in the enter of the membrane as there is water in the membrane 1
P4-Form a phospholipids bilayer ANY 3 1 3
(l) State the function of phospholipids in cells
Provide the structure basis for all cell membrane 1 1
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(l) Butter is saturated fat but corn oil is unsaturated fat . Explain briefly the differences between
saturated fat and unsaturated fat
P1- Saturated fat is solid (at room temperature) 1
P2-saturated fat without double bonds between carbon atoms where as unsaturated fat have at 1 2
least one doubled bond (between the carbon atoms)
4.5 Enzyme Trial Johor 2009 Trial Kedah 2012 Trial Kedah 2009 JUJ 2009
(c) Extracellular enzyme are enzyme which are produced in the cell but secreted from the cell to
function externally
Give one example of the cell
Pancreas//ileum //stomach epithelial cell //salivary cell 1 1
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Synthesis of enzymes Trail Johor 2011 Trail Kelantan 2010 Trial Pahang 2010 Trial MRSM 2011
Trail Perak 2012 Trial Perak 2009 Trial Selangor 2010 Trial Selangor 2008
R: secretory vesicles
P: Golgi apparatus
U : transport vesicles
T : Ribosomes
Q: nucleus
S: mitochondria
Enzyme is made up of chains of polypeptides molecule. The Golgi apparatus in living cell are
(g) malfunction .explain the effect of the malfunction to the production of extracellular enzyme
P1-Processing /modifying/packing/sorting/transporting of the synthesized protein could not be
carry out
P2-Resulting the synthesized protein /carbohydrates/glycoprotein are not able to be carry
1
through the plasma membrane
1 2
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(b) Based on the organelle shown in diagram, explain how extracellular enzyme are
produced starting at ribosome
P1-Protien are synthesized in the ribosome 1
P2-Rough endoplasmic reticulum transport the protein to the Golgi apparatus (by 1
transport vesicles)
P3Golgi apparatus modifies/repackage/transport protein to become enzyme 1
P4-which are secreted out from the cell 1 3
(b) Explain how the protein form at the ribosome is transform into the extracellular enzyme,
so that it can be secreted from the cell
P1-Protien enter lumen Rough Endoplasmic reticulum(RER) 1
P2-Modified in RER into extracellular enzyme 1
P3-Carried to the Golgi body by transporting vesicles 1
P4-Processed/packed in the Golgi body for excretion 1 3
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The mechanism of enzyme action Trial Kedah 2012 Trial Kedah 2009 JUJ 2009
Substrate X
Q:substrate
Substrate Y
(d) Explain why substrate Y, but not Substrate X, can combine with the enzyme
1
P1-substrate x does not have a specific active sites to fit into tha enzyme
1 2
P2-only Substrate Y , has shape which exactly fits into the enzyme
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(e) State the principle of enzymatic reaction as shown in Figure
key and lock
1 1
(f) The enzyme reaction is referred as the key lock hypothesis. Explain about hypothesis
P1-enzyem has active site where inly certain molecules can fit in
1
P2-substrate represents the key and enzyme represent the lock
P3-when the substrate fit into (active sites of ) enzyme forming enzyme-substrate be 1
complex 1
P4-enzyme catalyst the substrate to form products ANY 3 1 4
(g) A student carried out and experiment using enzyme X. he replaced lipid with maltose as
the substrate. At the end of the experiment, he observed that there was no reaction
1
P1-active site of the enzyme X is not complement to the shape of maltose
P2-maltose cannot bind to enzyme X 1
P3-no enzyme substrate complex is formed 1
P4-maltose is not hydrolysed /broken down 1 4
(h) A student carried out and experiment using enzyme X. he replaced lipid with maltose as
the substrate. At the end of the experiment, he observed that there was no reaction
1
P1-active site of the enzyme X is not complement to the shape of maltose
P2-maltose cannot bind to enzyme X 1
P3-no enzyme substrate complex is formed 1
P4-maltose is not hydrolysed /broken down 1 3
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The effect of temperature on the activity of enzyme Trial Johor 2011 Trial Kedah 2012 Trail Kelantan
Each enzyme has an optimum temperature at which its reaction rate is the fastest.
(a) beyond the optimum temperature an enzyme is said to be denatured
blood stain. the shirts were washed by using enzyme-containing washing powder at tow
different temperature, 35oCand 65oC
(d) Explain why washing of shirt in P is more effective when used with warm water 30oC-40oC
than cold water
1
P1-Enzyme are less active at low temperature
P2-Many enzyme have an optimum temperature range between 30oC-40oC 1 2
(e) Enzyme activity at low temperature
Explain the rate of enzyme reaction at low temperature and at the optimum temperature
1
P1-At low temperature , the enzyme is inactive and the rate of reaction Is slow
P2-at the optimum temperature, the enzyme is very active and the rate of enzyme reaction is at 1 2
maximum
(i) Draw a graph to show the effect of temperature on the activity of enzyme
Draw 1m(/) 1
Label 1m(/) 1 2
(ii) what is the optimum temperature for this reaction 1 1
37oC
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Starch ,protein and peptone can be hydrolyzed by enzyme amylase, pepsine and trypsin
respectively
Diagram shows the effect of pH an the rate of enzyme activity
Based on the diagram , explain how the changes in pH affect the enzyme activity 1
P1-Enzyme are denature by changes in pH level of the reaction medium 1
P2-Each enzyme function actively at its optimum pH 1
P3-he enzyme salivary amylase functions at the optimum pH 7.0. 1
P4-it will become inactive when its reaction medium is too acidic or alkaline 1
P5-the optimum pH for pepsin is 1.5-2.0and trypsin is pH 8.5 1
P6-the changes in pH will cause the changes in the concentration of hydrogen ion (H+)or
hydroxyl ions (OH-) 1
P7-the excess hydrogen ions or hydroxyl ions destabilize enzymes by changing the shape of 1
the active site
1
P8-enzyme stop functioning
1
P9-the effect of pH changes on the enzyme activity are reversible
1 10
P10-An enzyme which is inactive in a high pH will become active again when it is at its
optimum pH
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The effect of substrate concentration on the activity of enzymes Trial Melaka 2008 Trial perils 2011
62
Table 1 shows the enzyme substrate complex molecule at Q where the concentration of the
substrate is X%
P-1 1 2
R-1 1
Based on the graph in diagram 3.1, complete Diagram 3.2 by drawing the substrate molecule
(e) at region P and R
(f) Based on your drawing in diagram 3.2, explain the relationship of the substrate and enzyme
molecule at region P and R
1
P- P1-when the concentration of substrate increase, more substrate molecule bind to active
sites of the enzyme 1
P2-more product will be produced// the rate of reaction is directly propotional to the substrate
concentration 1
R: P3-after the maximum rate , all active sites of the enzyme molecule are filled/engaged 1
P4-the rate of reaction become constant
P5-the concentration of enzyme become the limiting factor 1 5
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(g) If the concentration of enzyme increase, draw a line to show the enzyme activity in diagram
3.3
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Chapter 4 : Chemical composition in the cell 2013
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(h) Explain your answer in (c)
P1-When the enzyme concentration increases, more substrate will bind to the active site of
1
enzyme
P2-the rate of reaction increase 1 2
The use of enzyme in daily life and industries Trial Johor 2009 Trial Kedah 2012 Trial Penang 2012
Biological enzyme are added to the washing power to boost its effectiveness
(a) Give one example of a biological enzyme of biological enzyme that can be added to washing
powder
Explain the action of this enzyme
F1-Protease/Lipase/Amylase 1
P2-to breakdown//dissolve/hydrolyses protein stain /fat stain / starch stain 1 2
(b) Name the enzyme present in washing powder to remove blood stain
Amylase/protease/lipase
1 1
(c) Suggest one type of enzyme would need to be present in a washing powder design to remove
blood stain
1 1
lipase/protease
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(d)
Give two example of enzyme present in biological washing powder that helps in the removal
of food stain from the clothing. State one function of each of the named enzyme 1
F1-Amylase- 1
P1-to hydrolyse starch stains
F1-Lipase 1
P2-to break down oily stains 1
F3-Protease 1
P3-to hydrolyse protein stain ANY 4 F+P 1 2
(e) Enzyme is widely used in daily life and in industries. explain the use of enzyme in the
process of extracting agar from seaweed
1
P1- Cellulase is added
P2-to digest the cell wall of the seaweed to extract agar 1 2
(f) A chef marinate meat with pineapple for 30 minutes before he cook the meat. Explain why
P1-protease/bromelian in fresh pineapple
1
P2-can be used to hydrolyse /tenderize the protein in the meat 1
P3-the meat is marinated with pineapple slices fro 30 minutes to give time for enzyme react 1 3
with protein in the meat ANY2
(g) Enzymes are widely used in our daily life and industries.
Explain how enzymes act in :
To get rid of stains on cloth:
F1- biological detergents contain proteases, amylases and lipases 1
P1- proteases acts on stains containing proteins / blood / saliva 1
P2- amylases acts on stains containing starch / sauces / ice cream/ gravy 1
P3- lipases are effective in removing oil and grease 1
Helping to cook meat:
F2 Protease acts on protein in meat 1 4
P4 tenderize / softens meat 1
(h) Explain example of the application of enzymes in cereal grains product industry
P1-lipase 1
E1-Ripening cheese OR 1
P2-Rennin 1
E2-Solidify milk potien MAX 2 1 2
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