Sudan IV Lipids Test and Paper Bag Test

In the first photograph

we tested the paper bag
test which is a sign for
lipids. Firstly we placed
one drop of water on the
first paper towel sheet
and a drop of fat on the
second towel sheet. As
you can see from the
before and after photos
the water is much less
staining when compared
to the fats added, which
have a deeper stain and
also spread farther on
the paper. In the after
photo you can see that the water has started to evaporate, which the fats have not, as well the
fats are relatively plain on the paper, while the water tends to warp the paper and leave the
paper warped after evaporating. The test results are positive for lipids on the bottom paper towel
sheet due to the coloration, and characteristics of lipids.
Sudan IV Test
In this Sudan IV test we used three test
tubes. The first was our control with 10
droplets of water and 5 droplets of oil,
which would be insoluble so there was a
level of oil on the top. While in our
second tube we added our control and 5
droplets of Sudan IV as the first part of
the lipids test. The third test tube was a
positive test when boiled in water the
Sudan IV and oil dissolved into each
other since both are nonpolar and
hydrophobic. Sudan IV stains lipids a
reddish-orange so our test result
concludes positive for lipids.