LCK 555 : Short Instruction BOD5

BOD5 Preparation

1. Dilution Water LZC 901 :

1.
Prepare Dilution 1. 500 ml tap water, free of chlorine
Prepare Dilution 20C 2
water
water 2. 0,3 ml trace element solution
Pump
3. Inoculation Source:......................
4. Inoculation Volume:.............. ml
Aeration! 5. After 1 h of aeration leave to stand for 1 h

2. A1 4 19 mg/l
2.
Choose the A2 7 38 mg/l No. Sample Measuring Range
Choose the
sampling A3 11 56 mg/l
sampling
Measuring B1 25 138 mg/l 1
Measuring
Range !!! B2 50 275 mg/l
Range !!!
B3 75 413 mg/l 2
C1 100 550 mg/l
C2 200 1100 mg/l 3
C3 300 1650 mg/l

3.
3.
Preliminary Sample Dil. Water Pipette into
Preliminary No. Sample Dil. Water reaction tube
dilution [ml] [ml]
dilution and shake
A1-3 4 ml ml vigorously
B1 1 1 for 1min.
B2 1 3 1
B3 1 5
C1 0,4 2,8 2
C2 0,4 6
C3 0,4 9,2 3

4.
4.
Pipette the
Pipette the in ml
diluted sample Fill the
diluted sample No. Pipette .... ml sample
into the cuvette with
into the A1 1,8
cuvette dilution water into the cuvette
cuvette A2 0,9
A3 0,6
B1 to
1
C3 je 0,5 2
3

Fill one cuvette with dilution water only.

Leave to stand for 5 days in darkness at 20 C. After 5 days follow the instruction
corresponding the pictos of LCK 555.