I. Evaluate the solubility of the following peptides at the given pH.

For each pair, which peptide is

more soluble and why? Hint: Evaluate the ionization states of the amino acid functional groups.
pH 7: Gly20 and (Glu-Asp)10

Jawaban:
Pada pH 7,0, gugus samping pada Aspartat dan Glutamat akan terionisasi. Kedua asam amino tersebut
juga menganfung gugus N dan C terminal yang terionisasi. Hal ini menyebabkan (Glu-Asp)10
memiliki gugus terionisasi lebih banyak daripada Gly20 karena pada pH 7 Glisin membentuk
zwitterion sehingga muatan positif dan negatifnya saling meniadakan, sehingga (Glu-Asp)10
merupakan peptida yang lebih mudah larut.

II. Match the terms below with the accompanying statements. (More than one term may apply to a
statement.)
A. dansyl chloride D. 2-mercaptoethanol
B. phenylisothiocyanate E. cyanogen bromide
C. iodoacetate
1) _____ An alkylating agent that reacts with Cys residues.
2) _____ A reducing agent that carries out reductive cleavage of disulfide bonds.
3) _____ A reagent used to identify N-terminal residues.
4) _____ A reagent that cleaves polypeptides into smaller fragments.
5) _____ A reagent used in sequencing polypeptides from the N-terminus.

Jawaban:
1. C
2. D
3. A
4. E
5. B

III. You have a polypeptide that has been degraded with cyanogen bromide and trypsin. The
sequences of the fragments are listed below (using one-letter codes). Is there enough information to
derive the complete amino acid sequence of the polypeptide? What further treatments might support
the deduced sequence?
Cyanogen bromide treatment:
(1) K
(2) RFM
(3) MLYCRGM
(4) NIKGLM
Trypsin treatment:
(5) FMK
(6) GMNIK
(7) GLMR
(8) MLYCR
Jawaban: Dibuat unit overlapnya
MLYCRGM
MLYCR
GMNIK
NIKGLM
GLMR
RFM
FMK
K

Maka urutan asam aminonya adalah MLYCRGMNIKGLMRFMK.