In the present paper the method of Van Slyke (1) for methemo-
globin determination has been modified by employing technique
1 The micro burette used for measuring the hydrosulfite and the acid
ferricyanide was made by sealing a stop-cock onto a pipette graduated in
0.01 cc. divisions. The delivery capillary was provided with a rubber tip
as shown in Fig. 3, p. 125 of Van Slykes paper (5) and described on p. 126.
D. D. Van Slyke and A. Hiller 207
that this volume of fluid is part of the total 2 cc. measured into the
chamber. Carbon monoxide to 150 mm. pressure is admitted into
the evacuated chamber in the same manner described above.
Of the acid ferricyanide solution only 0.05 cc. is added. It is run
in while the blood solution is still in the top of the chamber and is
followed by several drops of mercury which break up the methemo-
globin precipitate into fine particles. The procedure is continued
as detailed on p. 815 of our former paper (2).
The c correction for the micro method is somewhat over 20 mm.
EXPERIMENTAL.
I 1
Total Unoxidized
Quantity of CO capacity Methemoglobin
hemoglobin in hemoglobin in
blood used for of blood before in terms of
terms of co terms of co
smlysis. oxidation. CO capacity.
capacity. capacity.
cc. vol. per cent cd. per cent vol. per cent
2 18.41 18.34 9.01
18.40 18.51 8.93
18.47
_-
Average.. .. . 18.41 18.44 8.97 9.47
I 18.32 8.82
18.38 8.96
18.46
_
Average.. .. . 18.39 8.89 9.50
_
0.2 18.40 8.76
18.15 8.83
18.51