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ANSWER QUESTIONS

1. Illustrate the structure of dna!

2. How to determine the amount of isolated DNA using a

spectrophotometer?

DNA containing purine and pyrimidine bases can absorb UV

light. Double band DNA can absorb UV light at 260 nm, while protein
or phenol contaminants can absorb light at 280 nm. This difference in
absorption of UV light, so that the purity of DNA can be measured by
calculating the absorbance value of 260 nm divided by the absorbance
value of 280 (260 / 280), and the value of DNA purity ranges from
1.8-2.0. If the value exceeds 2.0 then the tested solution still contains
the contaminant from the membrane protein / other compound so that
the plasmid DNA content obtained is not pure. If less than 1.8 then
ddH2O is taken too much while the DNA taken is too little. The tools
used in this test with UV-Vis spectrophotometry methods are:
mikropipet, kuvet, nanodrop spectrophotometer and computer set.
Materials used are DNA isolate and aquadest as blank. The workings of
quantitative DNA testing begins by removing the nanodrop
spectrophotometer. Then do the blank test using aquadest. After that, it
was continued by testing the DNA isolates by dripping them on the
sample site as much as 1 microliter. Then measure the purity of DNA
and read the results of its DNA purity.