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RINGKASAN

RIFDAH APRIANTI. 135040200111061. Uji Plant Growth Promoting Rhizobacteria


(PGPR) Pada Pertumbuhan Tanaman Kacang Hijau dengan Media Tanam yang
Berbeda. Dibawah bimbingan Eko Handayanto dan Nur Laili.
Saat ini Tanaman kacang hijau masih dijadikan sebagai tanaman sampingan, sehingga
teknik budidayanya belum dilakukan secara optimal. Kelebihan dari tanaman kacang hijau
yaitu dapat ditanam pada kondisi tanah yang kurang subur seperti ultisol. Ultisol merupakan
salah satu jenis tanah yang tersebar luas sekitar 25% dari total luas daratan Indonesia. Ultisol
merupakan jenis tanah yang memiliki kandungan hara dan bahan organik yang rendah. Untuk
meningkatkan kesuburan tanah pada ultisol dan pertumbuhan tanaman kacang hijau pada
ultisol maka perlu dilakukan pengelolaan lahan seperti penambahan bahan organik dengan cara
pemupukan menggunakan pupuk organik hayati. Pupuk organik hayati merupakan pupuk yang
dibuat dari bahan-bahan organik dan mikroorganisme khususnya bakteri perakaran atau
dikenal dengan PGPR (Plant Growth Promoting Rhizobacteria). Bakteri PGPR dalam pupuk
organik hayati berperan penting dalam penyediaan unsur hara di tanah yang dibutuhkan untuk
mendukung pertumbuhan tanaman. Penelitian ini bertujuan untuk: 1) Mengetahui dampak
aplikasi isolat yang dijadikan formula pupuk hayati terhadap pertumbuhan tanaman kacang
hijau pada ultisol, 2) Mengetahui pengaruh aplikasi formula pupuk hayati terhadap sifat
biokimia tanah yang terdiri dari: total populasi bakteri penambat N, aktivitas respirasi, C-
organik, enzim urease, pH tanah dan ketersedian unsur hara N.
Penelitian ini dilaksanakan di Laboratorium Kimia Tanah Jurusan Tanah Fakultas
Pertanian Universitas Brawijaya, Laboratorium Mikrobiologi Pertanian dan Rumah Kaca LIPI
Cibinong. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL) Faktorial
terdapat 2 faktor perlakuan. Faktor 1 yaitu perlakuan media tanam yang terdiri dari 2 perlakuan
dan faktor 2 yaitu perlakuan formula pupuk hayati yang terdiri dari 4 perlakuan, sehingga
diperoleh 8 kombinasi perlakuan yang dilakukan 3 kali ulangan. Analisis tanah yang dilakukan
yaitu pH, C-Organik, N-Tersedia, Respirasi Mikroba, Enzim Urease dan Total Populasi Bakteri
Penambat N. Selain itu dilakukan pengamatan pertumbuhan tanaman yang terdiri dari tinggi
tanaman, BB dan BK brangkasan, BB dan BK akar, berat polong, jumlah bintil dan serapan N
tanaman.
Hasil penelitian menunjukkan perlakuan T2 dan H2 mampu meningkatkan populasi
bakteri penambat N, Respirasi, pH tanah dan enzim urease tanah, sedangkan perlakuan T2
mampu meningkatkan C-Organik dan N tersedia didalam tanah. Pada pertumbuhan tanaman,
rata-rata perlakuan media tanam T2 memberikan pengaruh yang lebih baik dibandingkan
dengan T1. Sedangkan pada perlakuan formula pupuk, perlakuan H3 memberikan pengaruh
yang lebih baik terhadap pertumbuhan tanaman, namun untuk berat polong perlakuan H2
memberikan pengaruh lebih baik dibandingkan dengan H3.
SUMMARY

RIFDAH APRIANTI. 135040200111061. Examination of Plant Growth Promotes


Rhizobacteria (PGPR) for Green Bean Plant Growth with Different Cropping Media.
Supervised by Eko Handayanto dan Nur Laili.
Green bean plants are still used as side plants, therefore the technique of green bean
cultivation itself has not been done with such optimum level. Green bean plants have some
advantages, one of which is they can be planted in less fertile soil conditions such
as ultisol. Ultisol is one type of soil that could be found in around 25% of Indonesia total
area. Ultisols has low-level of nutrients and organic matters. In order to improve soil fertility
and the growth of green bean plants, it is necessary to do soil management such as using
biological organic fertilizer. The source of organic biological fertilizer can be obtained from
bacteria in the root zone or so-called PGPR (Plant Growth Promoting Rhizobacteria) that plays
a role in providing nutrients to the soil. This research aims to: 1) find out the impact of the
isolates application that has been used as biofertilizer formula towards the growth of green
bean plants on ultisol type of soil, 2.) to understand the effect of biofertilizer formula
application against soil biochemical traits that consists of: total amount of N-Fixing bacteria,
organic carbon, N-nutrient availability and pH level on ultisol type of soil.
This research was conducted in Soil Chemistry Laboratory of Soil Department, Faculty
of Agriculture, Brawijaya University, Agricultural Microbiology Laboratory and LIPI
Cibinong Greenhouse. Completely Randomized Design (CRD) Factorial was used with two
factors of treatment. The first factor is planting media therapy consisting of two treatments and
the second factor that is the formula of biological fertilizer consisting of 4 treatments, so that
result 8 treatment combination done 3 times replication. Soil analyzes that have been performed
were pH, C-Organic, N-Available, Microbial Respiration, Urease Enzyme and Total
Population of N-Fixing Bacteria. In addition, the observation of plant growth consisting of
plant height, weight plant, oven dry weight plant, weight root, oven dry weight of the root,
weight pods, number of nodules and N-uptake of the plant.
The results showed that from eight treatments there are some divergence and different
responses on the growth of green bean plants and soil fertility on ultisol type of soil. T2H2
treatment can increase the population of N-fixing bacteria, Respiration, soil pH and
soil urease enzyme, T2H0 treatment can increase C-Organic and T2H1 treatment can increase
N available in the soil. In terms of plant growth, T2H3 treatment has a better effect than control.
Abstract
Green bean plants are still used as side plants, so the technique of green bean cultivation
has not been done optimally. The advantages of green bean plants that can be planted in less
fertile soil conditions such as ultisols. Ultisol is one type of soil that is widespread 25% of the
total area of Indonesia. Ultisols contain low nutrients and organic matter. To improve soil
fertility and the growth of green bean plants, it is necessary to do management of soil such as
fertilization with biological organic fertilizer from PGPR (Plant Growth
Promoting Rhizobacteria) which play a role in providing nutrients to the soil. The results
showed that from 8 treatments applied a different effect on the growth of green bean plants and
soil fertility on ultisol. T2H2 treatment can increase the population of N, Respiration, Soil pH,
and Soil Enzymes, T2H0 treatment can increase C-Organic (5.46%) and T2H3 treatment can
increase N available in the soil. In plant growth, T2H3 treatment has a better effect.