To be uploaded IGCSE Programme

to CIE. Biology 0610

Quiz
Name: ________________________ Class: ____ Date: __ / __ / _____ First Semester 2010
Topic: Enzymes & Food Tests Mark: out of [13] Duration: (15 min)
Answer all questions.
Question

The enzyme amylase speeds up the break down of starch into reducing sugar. For
Examiner's
Use
Amylase is present in saliva.

Equal volumes of starch and amylase solutions were mixed thoroughly in a container. A sample
was removed immediately and tested for presence of starch, protein and reducing sugar. The
remaining mixture was placed in a water- bath at 40 oC and left for one hour. During this time, the
contents were stirred with a clean glass rod at regular intervals. After one hour, a second sample
was removed and tested in the same way.

(a) Describe how you would carry out a simple test for starch and reducing sugar. In each case,
state the colour changes that would occur if the results were positive.

Test for starch

[2]

Test for reducing sugar

...

[3]

(b) (i) Record the results of the tests for the sample taken immediately after mixing the starch and
amylase solutions and for the second sample taken after one hour later. [3]

Test Sample tested immediately Sample tested after one hour

Starch

Protein

Reducing
Sugar

1
(ii) Suggest one reason why the water- bath used in this investigation was set at 40 oC. For
Examiner's
Use
. [1]

(iii) Suggest one reason why the mixture was stirred at regular intervals.

.. [1]

(c) In another investigation, the amylase solution was boiled and cooled and then mixed with the
starch solution. A sample was removed immediately and tested, as before. The remaining mixture
was kept at 40 oC and sampled again after one hour.

. [3]

[Total: 13]