Chapter IV
This chapter presents and interprets the data gathered in textual, graphical and
tabular manner.
Biological, RCNS, UST. The results show that the treatments released antibacterial
S. epidermidis Replicates
Mean
R1 R2 R3 Zone of Descriptive
Treatments
(mm) (mm) (mm) Inhibition Interpretation
(mm)
T1 Positive Control
34 34 34 34 Strong
(Cefoxtamine)
T2 Negative Control
Water)
T3 50% of Patolang
17 13 11 13.67 Moderate
Gubat Stem Extract
T4 75% of Patolang
20 17 18 18.33 Strong
Gubat Stem Extract
T5 100% of Patolang
25 23 24 24 Strong
Gubat Stem Extract
40
35
30
25
Replicate 1
20
Replicate 2
15 Replicate 3
10
0
Treatment 1 Treatment 2 Treatment 3 Treatment 4 Treatment 5
The descriptive interpretation of the results was adopted from Capal et, al, which
7 - 11 +, weak
0-6 Negative
The results show that that Patolang Gubat (Trichosanthes quinquangulata) Stem
the positive control (Cefoxtamine), has the highest average mean zone of inhibition of
34mm followed by the T5 which contains 100% of Patolang Gubat Stem extract with a
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General Santos City National High School
mean zone of inhibition of 24mm. this indicates that the Patolang Gubat Stem extract
epidermidis
This table shows the computed values based on the statistical test, One Way
ANOVA, which was performed to interpret the results for the Antibacterial Screening.
The Statistical Test indicates that the mean zone of inhibition of the different
are statistically different from treatment, which in the positive control. This means that
the four treatments are not effective against Staphylococcus epidermidis. On the other
hand Treatment 4 is not significantly different from treatment 1. This implies that 100%
present on the plant. The test was done in accordance with the Guidebook to Plant
Screening: Phytochemical & Biological, RCNS, UST. The results in this test are as
follows:
Phytochemicals Results
Alkaloids Positive
Flavonoids Negative
Glycosides Negative
Saponins Negative
Steroids Negative
Tannins Positive
Alkaloids and Tannins. It is also shown, based on the results that Flavonoids,
Glycosides, Saponins and Steroids are not present in the plant material.
Tannins is an antiseptic and astringent that affected the zone of inhibition of the
TLC was developed in six different solvents. Viewed under UV lamp and sprayed
with vanillin sulfuric acid. This test was done under concentrate condition. The results
for the Thin Layer Chromatography Test were expressed qualitatively and it is found out
10:1. No separation of components was observed in the rest of the developing solvents
and most of the components stayed at the original spot. The components of the
The results for the stability test done on the ointment are shown in the table. The
results include the evaluation of the ointment specifically on its colour, appearance and
consistency and pH level for a month. The ointment was observed three times for a
month.
Extract
Since there are no liquid formations, it indicates that the extract did not separate from
the base. First observation done on the ointment is 5.0 for the pH level. On the second
observation, the pH level rose to 5.1 and the third observation the pH level rose to 5.2.
This tells that the pH level of the ointment is within the acceptable range.
pH Level
5.25
5.2
5.15
5.1
pH Level
5.05
4.95
4.9
Trial 1 Trial 2 Trial 3
The table shows that the results for the first observation done on the ointment
are 5.0 for the pH level, it was greenish in color and uniform coloration with no liquid
formation was observed. On the second observation, the same results were gained
aside from the pH level which rose to 5.1. And on the third observation the colour of the
ointment was still greenish, uniform coloration with no liquid formation was also
observed but its pH level rose to 5.2. With respect to the scale of interpretation of pH
and chemical properties for a month. This tells that the ointment is safe and there are no
changes on its chemical properties that might affect its effectiveness and other
Results of the Antioxidant using 0.01 mM DPPH scavenging Assay of all Trials of
quinquangulata) stem extract using 0.01mM DPPH scavenging assay. The results in
1 0.286 22.70
2 0.290 21.62
3 0.290 21.62
The results for the three trials were 22.70%, 21.62% and 21.62% respectively.
The findings indicate that the Patolang Gubat (Trichosanthes quinquangulata) Stem
extract have inhibit the formation of DPPH at the percentage range of 21.62% to
22.70%.
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General Santos City National High School
% Scavenging Activity
22.8
22.6
22.4
22.2
22
21.6
21.4
21.2
21
Trial 1 Trial 2 Trial 3
This result tells that the plant extract can be an antioxidant agent however the
materials.