49

7. LAMPIRAN

LAMPIRAN 1. Pembuatan Reagen Bradford dan Larutan Standar Protein

1.1. Pembuatan Reagen Bradford

Commasive Blue sebanyak 0,01 gram dilarutkan ke dalam 5 ml etanol 95%
kemudain ditambah asam fosfor 85% sebanyak 10 ml, kemudian di saring dengan
menggunakan kertas saringdan disimpan dalam botol gelap pada suhu rendah.
Saat hendak akan digunakan maka harus dilakukan pengenceran sebanyak lima
kali.
(Bradford, 1976)

1.2. Pembuatan Larutan Standar Protein

Bovine Serum Albumin (BSA) sebanyak 25 mg dilarutkan ke dalam 10 ml
aquadest dan hasil pencampuran dinamakan larutan stock BSA 2500 ppm. Setelah
itu diambil 4ml dan diencerkan hingga 100 ml dengan menggunakan aquadest.
Setelah itu membuat pengukuran terhadap standar protein terlarut dengan
konsentrasi 0,10, 20,30, 40, 50, 60, 70, 80, 90,dan 100. Setelah itu diambil masing
masing 0,1ml larutan standar dari masing masing konsentrasi kemudian
ditambahkan dengan 5ml larutan Bradford dan didiamkan 5 menit,dan
dispektrofotometri dengan menggunakan panjang gelombang 595 nm.

49
 50

LAMPIRAN 2. Komposisi Larutan Kerja dalam Elektroforesis SDS-Page

Nama Larutan Komposisi

Larutan A 29,2 gram acrylamide
(Acrylamide Stock Solution), 100 0,8 gram bis-acrylamide
ml 30% acrylamide, 0,8% bis-
acrylamide
Larutan B 75 ml 2M Tris-HCl (pH 8,8) --- 1,5 M
(4x Separating Gel Buffer), 100 ml 4 ml 10% SDS --- 0,4 %
21 ml ddH2O
Larutan C 50 ml 1M Tris-HCl (pH 6,8) --- 0,5 M
(4x Stacking Gel Buffer), 100 ml 4 ml 10% SDS --- 0,4 %
46 ml ddH2O
10% Ammonium Persulphate 0,5 gram APS
(APS) 5 ml DDH2O
Buffer Elektroforesis 3 gr Tris --- 25Mm
14,4 gram glycine --- 192Mm
1 gram SDS --- 0,1%
ddH2O sampai 1 liter
Sampel Buffer 3,028 gram Tris
0,25 ml larutan C
0,4 ml 10% SDS
25 ml gliserol
0,2 mg bromophenol blue
0,031 gram Dithiothreitol (DTT)
Sumber : (Bollag et al., 1991 dengan modifikasi)
 51

LAMPIRAN 3. Kurva Standar BSA (Bovine Serum Albumin) Berat Molekular

Weight

3.1. Kurva Standar untuk Mie jahe konsentrasi 0% dan 1%

1.000

0.800
y = -1.0122x + 1.8568
0.600 R = 0.9635
Series1
0.400
Linear (Series1)
0.200

0.000
0.000 0.500 1.000 1.500 2.000
-0.200

3.2. Kurva Standar untuk Mie Jahe konsentrasi 2%

 52

LAMPIRAN 4. Analisis SPSS

4.1. Pengujian SPSS untuk Warna

Tests of Normality
a
Kolmogorov-Smirnov Shapiro-W ilk
perlakuan Statistic df Sig. Statistic df Sig.
L Mie Kontrol .186 6 .200* .903 6 .392
Mie Jahe 1% .158 6 .200* .969 6 .882
Mie Jahe 2% .333 6 .036 .845 6 .143
a Mie Kontrol .306 6 .084 .849 6 .154
Mie Jahe 1% .248 6 .200* .945 6 .696
Mie Jahe 2% .173 6 .200* .983 6 .964
b Mie Kontrol .197 6 .200* .975 6 .922
Mie Jahe 1% .189 6 .200* .868 6 .217
Mie Jahe 2% .183 6 .200* .961 6 .830
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

L
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Jahe 2% 6 53.3500
Mie Jahe 1% 6 66.6883
Mie Kontrol 6 71.4950
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.

a
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Kontrol 6 -1.0500
Mie Jahe 1% 6 -.6183
Mie Jahe 2% 6 .3700
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.

b
a
Duncan
Subset for alpha = .05
perlakuan N 1 2
Mie Jahe 2% 6 11.6600
Mie Jahe 1% 6 15.5400
Mie Kontrol 6 16.5633
Sig. 1.000 .307
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.
 53

4.2. Pengujian SPSS untuk Kelentingan

4.2.1. Tingkat Kelentingan pada Mie Jahe Formulasi A

Tests of Normality
a
Kolmogorov-Smirnov Shapiro-Wilk
perlakuan Statistic df Sig. Statistic df Sig.
Tensile_Strength Mie Kontrol .142 6 .200* .985 6 .972
Mie Jahe 1% .209 6 .200* .906 6 .414
Mie Jahe 2% .199 6 .200* .954 6 .774
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Tensile_Strength
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Jahe 2% 6 .01426500
Mie Jahe 1% 6 .02583083
Mie Kontrol 6 .05543833
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.

6.2.2. Tingkat Kelentingan pada Mie Jahe Formulasi B

Tests of Normality
a
Kolmogorov-Smirnov Shapiro-Wilk
perlakuan Statistic df Sig. Statistic df Sig.
Tensile_Strength Mie Kontrol .230 6 .200* .860 6 .187
Mie Jahe 1% .180 6 .200* .931 6 .591
Mie Jahe 2% .220 6 .200* .945 6 .697
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Tensile_Strength
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Kontrol 6 .01475633
Mie Jahe 1% 6 .02288317
Mie Jahe 2% 6 .03299517
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.
 54

4.3. Pengujian SPSS untuk Aktivitas Antioksidan

4.3.1. Aktivitas Antioksidan pada Mie Jahe Mentah

Tests of Normality
a
Kolmogorov-Smirnov Shapiro-Wilk
perlakuan Statistic df Sig. Statistic df Sig.
Inhibisi Mie Kontrol .187 6 .200* .939 6 .647
Mie Jahe 1% .266 6 .200* .900 6 .373
Mie Jahe 2% .265 6 .200* .861 6 .192
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Inhibisi
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Kontrol 6 7.6433
Mie Jahe 1% 6 27.7850
Mie Jahe 2% 6 49.5300
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.

4.3.2. Aktivitas Antioksidan pada Mie Jahe Matang

Tests of Normality
a
Kolmogorov-Smirnov Shapiro-Wilk
perlakuan Statistic df Sig. Statistic df Sig.
Inhibisi Mie Kontrol .241 6 .200* .900 6 .375
Mie Jahe 1% .237 6 .200* .952 6 .754
Mie Jahe 2% .187 6 .200* .933 6 .607
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Inhibisi
a
Duncan
Subset for alpha = .05
perlakuan N 1 2 3
Mie Kontrol 6 3.2733
Mie Jahe 1% 6 10.5083
Mie Jahe 2% 6 20.7933
Sig. 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6.000.