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Exchange Student Report

Oleh:

Zakiyatul Khoiriyah

JURUSAN BIOLOGI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS JEMBER
2017
Background

In September 2015, Biology department started bilateral programme with


Hochschule Flensburg University of Applied Sciences in capacity building for
student, staff and lecturer. This programme funded by DAAD (German Academic
Exchange Service) for 4 years. Workshop, exchange student, exchange staff
included in this programme.

Three of my friends and me get a chance to go to Hochschule Flensburg


University of Applied Sciences as student exchange. We was the second group
went to Flensburg. We registered as student in Biotechnology and Process
Engineering Faculty and conducted research with Protein Factory.

Orientation Week

In the beginning of September 2016, three of my friends and me get a


chance to go to Hochschule Flensburg University of Applied Sciences as student
exchange. We left early from Indonesia in winter semester in order to follow
whole activities since the beginning of the semester. Orientation week for
international student have started the winter semesters. Because it was very early,
only a few international students who attend, they are come from Turkey, Spain,
France, Namibian, Kenya and Indonesia. The first week we did the orientation
tour around the campus, register to courses using stud.ip, registered to town hall
and also tour to area around Schelswig-Holstein to know the history from the past.

Figure 1. International students from Turkey, France, Spain, Indonesia, Kenya and Namibian
Courses

Weeks later, the course has already begun. I took four courses for one
semester there, they are Protein Factory: Theory 1, theory 2, lab work and also
German language for foreign students. The courses highly structured, active and
effective within 4 months, we successfully completed the research, study and
exams. Outcome of the Theory 1 was poster, Theory 2 was conference and lab
work was manuscript, all three are interrelated.

Figure 2. Theory 2 about hybridoma cell line

Protein Factory Project

In 2010 Protein Factory group was formed with a target production of


monoclonal antibodies. During the study, we were asked to conduct research of
the animal cell line. There are two groups in the Protein Factory namely Upstream
Processing (USP), which is responsible for producing proteins and Downstream
Processing (DSP) which is responsible for purifying proteins that produced by
USP group.

During in FUAS, I joined the USP group to produce antibodies


(Immunoglobulin G) from CF-10H5 cell line. Based on the previous group
research, the results showed that the concentration of antibody still low, so we
plan to perform optimizations on culture medium to produce antibodies by using
Design of Experiment (DoE). We did the observation of the materials used for
medium culture from the previous research and showed that consumption of
glutamine and glucose by cells is very high and it can be seen in growth curve.
Glutamine and glucose was source of energy for cell growth and antibody
formation.

"Optimization of Immunoglobulin G (IgG) Production with a CF-10H5


Cell Line using Design of Experiment (DoE)" was the title we have chosen to
represent the research that we add. The first step was activated the cell line from
cryovial tube, and adapted them to low serum media in order not to affect the
quality of antibody during purification. After the cell adapt to the low serum
concentrations, we propagated the cells and did growth curve to find out cells
behavior. For the design of experiment we used software for the statistical
planning and the evaluation of experiments, it was called MODDE design wizard.
We have three factors i.e. glucose, glutamine and pluronic with three levels for
each factor, i.e minimum, medium and maximum. With these factors then it can
be 17 experiments with Central Composite Faced (CCF) Design in 1 run order.
After incubating for 7 days, we measured the consumption of glucose, glutamine,
lactate formation and also the concentration of IgG by ELISA. The result showed
that glucose, glutamine and pluronic have a strong influence in the production of
IgG. The influence of glucose and glutamine was directly proportional to the
production of IgG while pluronic inversely.
A B

Figure 3. A. Counting cell concentration. B. Doing ELISA for analysis IgG concentration

Scientific Conference and Poster Presentation

The results of this experiment presented at the scientific conference which


was held on December 15, 2016 and also posters presentation on December 20,
2016. The results were also published in manuscript that is still the process of
review. Examinations were also performed to obtain the value of the courses.

Scientific conference was held by Protein Factory group, Food and


Biotechnology group and Water group. Protein Factory group presented about
production and purification of monoclonal antibody, Food and Biotechnology
group presented about non-alcoholic beverage from malt, Water group presented
about sea water become drinkable water. Professors, master students, bachelor
students, lab school students, technicians and also some teachers from high school
were attended to this conference.

Figure 4. Professors, Technicians and Master students from Biotechnology and Process
Engineering in Scientific Conference

International Student Activity

Not only followed courses, but also we joined some event for International
Student.

1. Trip to Laboe Naval Memorial

The Laboe Naval Memorial (a.k.a. Laboe Tower) is a memorial located in


Laboe, near Kiel, in Schleswig-Holstein, Germany. The monument originally
memorialized the World War I, war dead of the Kaiserliche Marine, with the
Kriegsmarine dead of World War II being added after 1945. In 1954, it was
rededicated to commemorate the sailors of all nationalities who died during
the World Wars.

The monument consists of a 72-metre-high (236 ft.) tower topped by an


observation deck. The deck stands a total 85 m (279 ft.) above sea level. A
hall of remembrance and World War II-era German submarine U-995, which
houses a technical museum, both sit near the foot of the monument, and the
site is a popular tourist venue. U-995 is the worlds only remaining Type VII
U-boat.
A B

C D

Figure 5. A. Laboe Tower B. German submarine U-995 C. Inside Laboe Tower D. International
Students in front of Laboe Tower

2. Trip to Freilichtmuseum Molfsee

Over 60 historic buildings, farms and mills of the different landscapes of


Schleswig-Holstein can be experienced on the 40-hectare site with meadows,
gardens, field sand ponds. Furniture, household items, instrument, animals
were kept in the courtyards, make this one of a kind excursion destination in
Schleswig-Holstein.

Figure 6. International students were in Freilichtmuseum Molfsee


3. Trip to Glcksburg

The House of Glcksburg is a Dano-German ducal family and a branch of


the House of Oldenburg, members of which have at various times reigned in
Denmark, Norway and Greece. Glcksburg Castle is a water castle
(Wasserschloss) in the town of Glcksburg, Germany. It is one of the most
important Renaissance castles in northern Europe. It is the seat of the House of
Schleswig-Holstein-Sonderburg-Glcksburg and was also used by the Danish
kings. Situated on the Flensburg Fjord the castle is now a museum owned by a
foundation, and is no longer inhabited by the ducal family. Its board of
directors is chaired by Christoph, Prince of Schleswig-Holstein, the current
titular duke and head of the House of Glcksburg and House of Oldenburg.

A B

Figure 7. A. Glcksburg Castle B. I was posed in front of Glcksburg Castle

4. City Tour
City tour was held by ASTA (student assosiation) for the first stop, we
going to Phanomenta, the science centre, Planetarium, and also we were went
to Denmark to buy hot dog and ice cream, but I can not eat them so I bought
fried fries.

5. International Dinner

We were held International dinner in Rotunde. All international students


were cooked special dishes from each country and introduced them to the
other student. My friends and me from Indonesia had cooked Terong Balado,
rice and omelett. We were very happy when the other students love our
dishes. I was also try dishes from Turkey, France, India, Spain and Germany.
We had a lot of fun and delcious dishes.
Figure 7. International dinner

6. Trip to Lbeck

Lbeck is a city in Schleswig-Holstein, northern Germany, and one of the


major ports of Germany. On the river Trave, it was the leading city of the
Hanseatic League, and because of its extensive Brick Gothic architecture is
listed by UNESCO as a World Heritage Site. The old part of Lbeck is on an
island enclosed by the Trave. The ElbeLbeck Canal connects the Trave
with the Elbe River. Another important river near the town centre is the
Wakenitz. The Autobahn 1 connects Lbeck with Hamburg and Denmark.
Travemnde is a sea resort and ferry port on the coast of the Baltic Sea.
Lbeck Hauptbahnhof links Lbeck to a number of railway lines, notably the
line to Hamburg.

Lbeck is famous for its marzipan industry. According to local legend,


marzipan was first made in Lbeck, possibly in response either to a military
siege of the city or a famine year. The story, perhaps apocryphal, is that the
town ran out of all food except stored almonds and sugar, which were used to
make loaves of marzipan "bread". Others believe that marzipan was actually
invented in Persia a few hundred years before Lbeck claims to have invented
it. The best known producer is Niederegger, which tourists often visit while in
Lbeck, especially at Christmas time. Unfortunately, Lbeck was foggy and
very cold so we can not see Holstentor and old building clearly.