Sysmex XS-1000i Automated Hematology Analyzer

Red Blood Cells and Platelets - RBCs and PLTs are counted in a dedicated channel using the
direct current detection method with hydrodynamic focusing technology to minimize
coincidence or recirculation. Automatic discriminators separate the two cell populations
based on complex algorithms. The intensity of the electronic pulse from each analyzed RBC
is proportional to the cell volume. The hematocrit (HCT) is directly determined based on the
count and volume detection of each individual RBC. Even with samples at extremely low or
unusually high concentrations, the Sysmex cell counters analyze RBCs and platelets with
uncompromised precision and accuracy.

Hemoglobin Analysis - The Sysmex sodium lauryl sulfate (SLS) method for hemoglobin
analysis is a non-cyanide method. Hemoglobin is determined in a separate channel,
minimizing interference from high leukocyte concentrations. The SLS method shows
excellent correlation with the reference method. With very short reaction times, this method
is ideal for high throughput analyzers.
Fluorescent Flow Cytometry - Traditional flow cytometry is considered the best method for
the differentiation of cell populations. However, traditional flow cytometry requires the use
of costly antigen-antibody reagents and the procedures can be cumbersome and time
consuming. Fluorescent technology enables the XS-1000i to reliably differentiate normal
WBC populations from abnormal WBC populations. The sensitivity of the unique
application of fluorescent flow cytometry gives the lab a high level of confidence in reporting
accurate WBC Differentials, even on critical patient samples when the WBC count is low.

WBC Differential - Fluorescent labeling is a milestone for the routine WBC differential.
Fluorescent measurement reveals the nucleus-plasma ratio of each individually stained cell,
enabling the XS-Series analyzers to differentiate five reportable WBC populations. The
combination of side scatter (inner complexity of the cell), forward scatter (volume) and
fluorescence of nucleic acid material determines the classification of each WBC. The XS-
Series utilizes an Adaptive Cluster Analysis System (ACAS) instead of conventional
discriminators to separate cell populations into well defined clusters. This three-dimensional
measurement of WBC provides an accurate and precise differential, even in highly
pathological samples.

https://www.sysmex.com/us/en/Products/Hematology/XSSeries/Pages/XS-Series-Hematology-
Analyzers.aspx