Inflammation of the
mammary gland…
Gland Sinus
Udder Skin
Teat Sinus
Teat Wall
Teat Canal
Causes of mastitis
Sometimes very
sick…
– Reduced yield
– Temperature drop
– Vascular collapse
– Death
Why is mastitis important?
• Common (worldwide)…
• Serious repercussions…
Common…
>50 cases/100
• Clinical mastitis
cows/yr
350
300
Incidence rate of clinical mastitis
(cases per 100 cows / year)
250
200
• Subclinical
150
mastitis
100
0
Farm in ascending order of incidence rate
Repercussions…
• Cost
– 40% direct costs of common diseases
– £200M UK (US$2.0B )
• Public health
– Bacteria in milk
– Antibiotic use
• Welfare
– Farm animal welfare council
• Sustainability
– Wastage, costs, environmental impact…
My Journey
Control of mastitis
Prevent infections
Management interventions
Vaccines may come but difficult to date…
Initial studies on control…coincidence
2 Months 10 Months
Simple dry period study
2 2 C M1 M2 M3
E coli.
Isolates: 2 weeks pre-calving
Calving
Mastitis: Week 8
Week 9
Week 10
DNA Fingerprinting - Cow 744 B (RH)
M 4 3 2 1 M1 M2 M3 E. coli
Isolates: 4 weeks pre-calving
2 weeks pre-calving
1 weeks pre-calving
Mastitis: Week 1
Week 7
Week 10
DNA Fingerprinting - Cow 167 S (LH)
M 2 1 M
E. coli
Isolates: 2 weeks pre-calving
1 weeks pre-calving
Mastitis: Week 2
Changing behaviour of environmental bacteria
M -26-12-5 2 41 62 83 -15 -8 -3 2 -1 0 1 -1 0 1 -1 77 -6 3 M
Bradley A.J. and Green M.J. (2001) Adaptation of Escherichia coli to the Bovine Mammary Gland.
J. Clin. Microbiol. 39;1845-9.
It turned out to be important…
Green M.J., Green L.E., Medley G.F., Schukken Y.H., and Bradley A.J. (2002). Influence of Dry
Period Bacterial Intramammary Infection on Clinical Mastitis in Dairy Cows. J. Dairy Sci. 85:
2589-2599.
Mastitis in the first 30 days of Lactation
50.00%
45.00%
Incidence Rate of Clinical Mastitis
40.00%
(Cases per Cow at Risk)
35.00%
30.00%
25.00%
20.00%
15.00%
10.00%
5.00%
0.00%
Farm-years in Ascending order of Clinical Mastitis Incidence
Research into Industry
…Prevention?
Preventive
Treatments
Pathogen, Cow, Farm,
Management
Dry Cow Treatment: A Clinical Trial
Bradley A.J. and Green M.J. (2001) An investigation of the impact of intramammary antibiotic dry
cow therapy on clinical coliform mastitis. J. Dairy Sci. 84 1632-1639
Non-Antibiotic Approaches
Huxley J.N., Green M.J., Green L.E., and Bradley A.J. (2002). Evaluation of the Efficacy of an
Internal Teat Sealer During the Dry Period. J Dairy Sci. 85: 551-561.
Follow on research
Preventive
Pathogen, Cow, Farm,
Treatments
Management
Determinants of Mastitis arising from the Dry
Period
Bacterial Dry
Interactions Period
Decisions
Further studies
Bacterial communities…
Cow and management factors
• Previous Lactation
• Drying Off
• Early Dry period
• Late Dry Period
• Calving Period
• Overall herd factors
8000kg ~ 10m2
Pasture grazing policy is:
Late Dry Period
Graze for 2 weeks then
rest 4 weeks
↓ risk
Farm tool for dry period management
Therefore
Action
Points
“Diagnosis”
Must
Should
Could
Randomised clinical trial
0
Proportional Change in Incidence
-0.05
Rate of Cows Affected
-0.1
-0.15
-0.2
-0.25
-0.3
-0.35
-0.4
Control Compliance Compliance Compliance
Farms < 1/3 1/3 - 2/3 >2/3
Could this be used on a widespread scale?
Action
“Diagnosis” Points
Must
Should COMPLIANCE
Could
Pilot study
20 vets selected by
their farmers
A National Mastitis Control Scheme
• In October 2008 – nationwide…
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Page 30!
Profile?
Virulence and
Molecular
vaccines
epidemiology (strain
Host pathogen
specific infection
interactions patterns)
Understanding infection
patterns to prevent mastitis
Optimising
Risk factor decisions on
studies disease
prevention
• Andrew Bradley
• James Breen, Chris Hudson, Katherine Leach, Laura
Green, Jon Huxley, Aurelien Madouasse
• Jamie Leigh, Tracey Coffey, Richard Emes, Jasmeet
Kaler
• Funders
– Wellcome Trust
– DairyCo
– BBSRC
– Leo AH
– Bimeda AH
– Pfizer AH