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Microscopy: Parts & Observations 9

Name: _TEACHER'S NOTES_ Period: ____

1. What are the parts? Label the parts of a microscope on the diagram
below.

Eyepiece

Body tube

Nosepiece
Arm
Low power
Med. Power
Stage
High power

Stage clips Coarse Adj.

Diaphragm Fine Adj.

Light source
Base

WORD BANK
Arm Eyepiece Nosepiece
Base Fine adjustment Stage
Body tube High power objective Stage clips
Coarse adjustment Light source Med. Power objective
Diaphragm Low power objective

1 Minority Science Programs – School of Biological Sciences – University of California, Irvine

2. What do the parts do? Match the part of the microscope with its
function.
_5_ Where the slide is placed 1.
Objectives
_4_ Used to focus when using the low power objective 2.
Base
_1_ Magnifies the specimen 3. Arm
_3_ Used to carry microscope 4. Course
adjustment
_7_ Used to focus when using the high power objective 5.
Stage
_6_ The lens you look through, magnifies the specimen 6.
Eyepiece
_2_ Supports the microscope 7. Fine
adjustment

3. Magnification Calculate the total magnification for each objective.

Eyepiece: 10X Objectives: 4X, 10X, or 40X
4X objective: __10__ x __4__ = __40X__
10X objective: __10__ x __10__ = __100X__
40X objective: __10__ x __40__ = __400X__

4. Letter “e” Observe the letter “e” with your naked eye and the
microscope. Draw only what you see through the eyepiece. Include the
correct magnification.
 Naked eye _______ X

What do you observe about the orientation and direction of the image?
___In the eyepiece, the “e” appears to be upside-down
and backwards.__

5. Comic strip Observe the colored comic strip with your naked eye and the
microscope. Draw only what you see through the eyepiece. Include the
correct magnification.

Naked eye _______ X

6. Prepared Slides Observe a prepared slide at different magnifications.

Record the title of the slide. Draw only what you see through the eyepiece.
Include the correct magnification.
Slide title: __________________________
 _______ X _______ X
Slide Preparations:
Letter “e”: Make a class set of letter “e” slides by printing out a page of tab-
delimited e’s. Cut out each letter and secure them to slides using clear
nail polish and a cover slip. Let dry overnight before using.

Comic strip: Cut out small pieces of colored newspaper comic strips. Have
students place them on a slide with a cover slip when viewing under
the microscope.

Prepared slides: Use a commercially available set of assorted prepared

slides.

Cheek cells: Have each student scrape the inside of their cheek using a
cotton swab. Smear the swab on a clean slide to dislodge the cells. Add
a drop of bromothymol blue stain and cover with a cover slip. Disinfect
used slides with bleach. Only allow students to handle their own cheek
cell slides.