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    Anatomi Dan Fisiologi Yang Berhubungan Dengan Konstruksi G

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    Anatomi Dan Fisiologi Yang Berhubungan Dengan Konstruksi G

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    13 tayangan12 halaman

    Chitosan PDF

    Diunggah oleh

    kusno
    Anatomi Dan Fisiologi Yang Berhubungan Dengan Konstruksi G

    Hak Cipta:

    © All Rights Reserved
    Unduh sebagai PDF atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
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    International Journal of Nanomedicine Dove; 3a ORIGINAL RESEARCH Improving effects of chitosan nanofiber scaffolds on osteoblast proliferation and maturation Ming-Hua Ho! Mei-Hsiu Liao? Yi-Ling Lin? Chien-Hao Lai? Pei Lin? Ruei-Ming Chen?“ "Department of Chemiesl Engineering Nadonal Taiwan Univerieyof Science and Technology, Tapes, Tawa Cal Physiology and Molecular Ima Rerearch Center ane Departme ‘Anesthesiology, Wan Fang Hospital Graduate Institute of Medical Sciences, Taipei Medical University, ‘Talpel.Talwan;‘Anestheties and ‘Toxicology Retearch Center 7sipeh Medical Unversity Hospital Tape, of Correspondence: Rushing Chen Graduate Inset of Medial Sciences, College of Medicine, Tape Media! Universivy 250 Wu Xing St, Tape 110, Taiwan Tol +666 2.2736 1668 one 3202 Fr 18862 8662 1119 Email emchen@umedutw Abstract: Osteoblast maturation plays a key role in regulating osteogenesis. Eletrospun rnanofbrous products were eported to possess a high surface area and porosity. In this study, we developed chitosan nanofibers and examined the effects of nanofibeous scaffolds on osteo: ‘last maturation and the possible mechanisms. Macro- and miero observations of the chitosan nanofibers revealed tht these nanoproducts had a at surface and well-distibuted fibers with nanoscale diameters. Moute osteoblasts were able to alach onto the chitosan nanoSiber sca folds, andthe scaffolde degraded ina time-dependent manner. Analysis by scanning electron ictoscopy further showed mouse osteoblasts adhered onto the seaffolds along the nanofibers, annd cell-cell communication was also detected, Mouse osteoblasts grew much bette on chitosan nanofiber seaffolds than on chitosan films, In addition, human ostecblasts were able to adhere and grow on the chitosan nanofiber scaffolds, Interestingly, culturing buman osteoblasts on. chitosan nanofiber scaffolds ime-dependently increased DNA replication and cell proliferation {In parallel, administration of human esteoblasts onto chitosan nanofibers significantly induced costeapontin, esteocalein, and alkaline phosphatate (ALP) messenger (mRNA expression. AS to the mechanism, chitosan sanosibers triggered runt-rclated tanscrption factor 2 mRNA and protein syntheses. Consequently, results of ALP-,alzarn ed and von Kossa-taining analyses showed that chitosan nanofibers improved ostecblast mineralization. Taken together, results of this study demonstrate that chitosan nanofibers can stimulate osteoblast proliferation and maturation via runt-telated transcription factor 2-mediated regulation of osteoblast associated costeopontn, osteocalcin, and ALP gene expression, Keywords: chitosan nanofibers otecblast-associated gene expression, osteoblast maturation, Rum Introduction Bone contributes to tissue protection, physical movement, and skeletal support. In the clini, hone diseases suchas bone defects and fractures in patients may lea to disability or even death. Anatomically, the bone structure is maintained by bone remodeling, Which is a balanced and dynamic process of bone formation and resorption! Osteo- blasts, which differentiate from stromal stem cells, were reported to play a central role in mediating bone formation » Osteogenesis is the process of bone formation and consists of various consecutive stages, including osteoprogenitor proliferation, matrix maturation, and osteoblast mineralization Inthe beginning of bone turnover or frac- ture healing, stem cells and primitive osteoprogenitors proliferate and subsequently replace old and damaged osteoblasts. These precursor cells successively undergo sequential differentiation and finally achieve osteablast maturation and bone nodule formation. However, multiple factors participate in controling osteoblast maturation. Thus, to develop innovative biomaterials for therapy of hone diseases, itis important IngeratiorlJooral of Nanomedicine 10149 43-0004 4293 RS feist ra inte hin Sin act eect Saeco ls fran eter npr a eee dasa yl an Cio npe one onset ignoring Speco Ho cea Dovey to evalvate the mechanisms of how biomaterials regulate ‘osteoblast development ‘A complicated network of molecular events is involved in regulating bone development." These progressive events axe tightly regulated by sequential induction of osteablast

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