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pH/REF/ORP ELECTRODES
OPERATING INSTRUCTIONS
PREPARATION FOR USE
All sensors are shipped with the measuring end
covered with a soaker teat. Before use, remove
the soaker teat from the electrode (keep this in
a safe place for future long term storage) and
rinse the measuring end with deionised water.
The electrode will now be ready for use.
Note: During shipment it is possible for air
bubbles to move into the glass bulb. If this has
occurred, shake the electrode in the same
manner as a clinical thermometer until the
glass bulb is filled with solution.
ELECTRODE STORAGE
pH glass electrodes should be stored in a pH 4.0
buffer solution for both overnight and long
term storage.
Single junction type reference and glass/plastic
electrodes should be stored in a 3 M KCI
solution (pH adjusted to 4.0).
Double junction types should use a 3.0 M
solution of the appropriate salt.
ELECTRODE CARE & CLEANING
Slow response and non-reproducible
measurements are indications that the
electrodes may have become coated or
clogged.
Typically 95% of the final reading should be
achieved within 10 seconds. If the glass has
become coated or clogged the time taken to
perform a measurement will increase. Rinsing
with methanol should remove the coating and
restore the original response speed. If rinsing
with methanol does not restore the response it
should be restored as follows:
1. Soak in 0.1 M HCI for 5 minutes
2. Remove and rinse with water
3. Place in 0.1 M NaOH for 5 minutes
4. Remove and rinse with water
5. Soak in pH 4.0 buffer for at least 10
minutes
before use.
O.R.P electrodes may require additional
cleaning from time to time with crocus paper.
By continuously using a pH electrode above
60°C the outer layer of the pH glass loses its
sensitivity. The sensitivity can be restored as
follows:
1. Prepare a 10% solution of ammonium
bifluoride.*
2. Immerse the electrode for 10 - 20
seconds.
3. Rinse in water.
4. Immerse in 5 M HCI for 5 minutes to remove
any excess bifluoride.
5. Rinse in water.
6. Soak in pH 4.0 buffer for at least 1 hour
before use.
* Ammonium bifluoride is a hazardous
substance and should therefore only be
handled by qualified personnel.
Note: pH electrodes have the optimum
response after being stored in slightly acidic
solutions e.g. a pH 4.0 buffer.
Reference electrodes use a liquid junction for
electrical contact to the solution being
measured. The reference will become erratic if
this junction becomes clogged or coated.
Periodic cleaning with methanol or 0.1 M HCI
will enhance the electrodes performance.
Reference electrodes should be stored in a
slightly acidic 3.0 M KCI solution.
COMBINATION pH ELECTRODES
As these are a combination of reference and
pH glass electrodes they should be cleaned as
described above and stored in slightly acidic 3.0
M KCI solution.
FILLING SOLUTIONS
Reference Electrodes - Saturated KCI
Combination pH/ORP Electrodes-3.0 M KCI
saturated with AgCI.
To fill the electrode, slide down the plastic ring
to reveal the hole in the side. Using a small
syringe, fill the outer part of the electrode with
the filling solution detailed above. Once the
filling solution is level with the hole slide the
plastic ring back to recover the hole.
Note: Certain combination electrodes are filled
with KCI gel and therefore do not require
filling. These electrodes have no filling hole.
061 663/REV A/08-08

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