PRIME TECH.LTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05 ELY 4y%ob 2490 Y=taL—-3 Piezo impact Micro Manipulator INSTRUCTION MANUAL C SYSTEM PMM-150FU ] (1) PMM CONTROLLER, OPERATION BOX PMAS-CT150,0P-15,0P-16 (2) ONE DIMENSIONAL PIEZO IMPACT DRIVE UNIT MB-U | Please read this manual carefully before using the PMM. | (The Piezo impact Micro Manipulator). PRIME TECH LTD, JAPANPRIME TECH.LTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05 {C CONTENTS j Prior to Operation - 3 For safe Operation 4 1. Introduction = — G 2. Unpacking wma —— 5 3. Specifications ccc eG Functional Outline : = on Changing the Joint Unit ee : 6 4. System Configuration: —— 7 5. Name and Funetion of Each Part =~ 8 (1) PMM Controller (PMAS~CT150) 8 (1-1-A) Front Panel 8 (1-1-8) Rear Panel 9 (1-2) Operation BOX (OP—15) 10 (1-3) Foot Switch (OP—16) 12 (2) One Dimensional Piezo Impact Drive Unit (MB—U) 13 6. Installation eee vi 15 (1) Installing the Controller 15 (2) Installing the Piezo Impact Drive Unit (MB—U), 15 Ext) For Narishige manipulator AD—NP 16 x2) For Narishige manipulator AD—NSL 18 Ex3) For Eppendorf manipulator 19 Exd) For Leica manipulator AD-LBK 20 2Separating the Impact Unit from Exclusive Injection holder 22 7. Method of Use rmmnninninnnnn ee cc 23 8. Truble~shooting etn sn 25 9. Maintenances — —————= 28 10. Limited Warranty smn s a ; 30PRIME TECHLTD, = Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05: Prior to Operation | Before operating this product, be sure to fully read and understand this instruction manual. | Store this manual close at hand so that you have access to it when required. ™ Observe all usage procedures unique to this product as well as all usage procedures specified in the manual. ™ Be sure to understand and follow all instructions in this manual concerning safety. « Instruction manual ) ™ This manual describes the names and functions of individual components, and assumes that the Piezo Impact Drive Unit (MB-U) is mounted on a three dimen- sional micro-manipulator similar to the one shown at right. | The contents of this manual are subject to change without notice due to product improvements. This manual is copyrighted by PRIME TECH LTD. None of its contents shall be transmitted or otherwise duplicated without prior permission from PRIME TECH LTD. This manual is lost, contact PRIME TECH LTD. at the following address. = We have made every effort to confirm the accuracy of information contained in this manual, but should you find any ambiguities, errors or omissions, please contact PRIME TECH LTD. at the following address. ‘= In the event that errors or omissions are found, we regret that we may not be able to correct them immediately. {Responsible for contents) PRIME TECH LTD. 635 Nakamukaihara, Tsuchiura-shi, Ibaraki-ken, 300-0841 Japan Tel +81-29-830-4517 Fax +81-29-830-4515PRIME TECH.LTD. Pi 0 impact Micro Manipulator [PMM-150FU] ‘0707-05 For Safe Operation This manual uses the following indications to warn against potential injury or property damage. Their meanings are shown below. Be sure to familiarize yourself these indications before reading ‘the manual. \Caution! Incorrect operation of product may lead to serious or major injured. (A\Please Note! Incorrect operation of the product may lead to minor injury or property damage. ! Important, Incorrect operation of the product may lead to operational errors. Cautions and Notes Carefully read and observe all the following cautions and notes, to ensure that you operate this product safely and correctly. ™ Usage © This system is designed for research and measurement, and cannot be used for other Purposes. Carefully read and understand this manual and follow all instructions in order to prevent danger @ Installation (A\Please Notel] © Use the attached power cable or a commercially available power cable that conforms to all relevant national laws and standards. The cable should be less than 3m long, © Install a power outlet within reach of operator, e.g... on the table supporting the product's controller. In the event anything abnormal happens, unplug the power cord to cut off the Product's supply. During normal operation, use the power switch to tum the power supply on and off. Environmental conditions (\Please N © Install this system inside a dry room having low levels of atmospheric dust and free from corrosive or flammable gases; room temperature should be maintained at ordinary levels. ez © Use a stable commercial power supply of 100 to 240 VAC and 50/60Hz, © POLLUTION DEGREE 2 @ TEMPERATURE 5°C to 40°C © OVERVOLTAGE CATEGORY IIPRIME TECHLTD. _Piezo impact Mioro Manipulator [PMM-150FU] ‘0107-05 1. Introduction ‘As the biotechnology industry grows, there is an increasing demand for advanced technologies. One example of this is the micro-manipulation of mammalian cells, which is becoming a fundamental and important technology. The PMM ( Piezo Micro Manipulator ) was developed to facilitate micromanipulation by utilizing @ rapid deformation of piezoelectric elements. This original driving method, represents ‘an improvement on conventional pipette manipulation units, such as those driven mechanically Applications Micro manipulations in embryology engineering, cell engineering, gene engineering, obstetrics and gynecology, electric physiology, nervous physiology, etc. For example; electrophysiology, neurophysiology, nuclear transfer, embryo biopsy, micro fertilization, foreign gene transfer, production of chimeras, measurement of micro potential, PMM is applicable to cells from many species, including livestock (cattle, horse, swine, sheep, goat, etc.) laboratory (rabbits, rats, hamsters, mice, etc.) poultry, plants, fishes, ete. 2. Unpacking This product incorporates precision parts and electronics. Handle these components carefully. (1) Unpack the product so as not to damage the articles inside. (2) See 4, "System Configuration” on page 7 of this manual to confirm that all components are Present. If any component is missing or appears abnormal, contact PRIME TECH LTD. offices or ‘your local distributor. (3) Make sure that no small parts are left in the packing material “IPRIME TECH.LTD. 3. Specifications Piezo impact Micro Manipulator [PMM~150FU] ‘0707-05'. SYSTEM PMMCT50FU = Output, 1 TE i age eee eet SM Moving range "Approx. = Smi —— © Traveling speed 0108 a's (MAX) Traveling resolution : Approx. 0.4m (MIN) Aqustment dial :2 set (Speed=16 levels, Intensity Condition Indicator lamp : 2 set (Condition A (green), Condition B (yellow) |] Condition selection switch Horizontal traveling keys ‘Arrow pedal to move left (Left pedal) Condition selection pedal (Right pedal) | 240w) x 340(0) x 140(H)nm Weight approx. 5 Oke 100KW) x 160(0) x GOH) Weight approx. 530g (including wire) IMPACT UNIT ; Weight approx. 125g (including wire) SLIDE BASE UNIT ; Weight approx. 75g (It dose not include the doin Unit) 140(W) x 1 10(0) x 35¢t)mm. Weight approx, 620g (including wire) [5-40 0-905 16 levels) {0 = 2000 m Sy guia 5] AC 100 - 240 V, 50760He . COVA Scie <_| Spare fuse(T3.15A, 250V), (TIA. 250V), Power cord 5 The above dmoncions do not include protrusions (FUNCTIONAL OUTLINE] SYSTEM [PMM-150FU) Super precision ‘The {MB-U} is one dimensional type (with power supply and moving element ) linear drive unit, ‘The (MB-U) provides a super-precise minimum resolution of 0.itm. ™ Centralized control at your side System [PMM-150FU] has an operating section at your side, incorporating a power supply and control section and which is separate from the main controller. * An easily accessible dial on the operation unit. This allows easy setting of PMM' s operating speed and intensity by hand during operation, The switches on the operation unit enable the operator to remotely control the manipulator with high precision without turning his or her eyes from the microscope, + Afoot switch toggle permits hand-free operation. Easy toggle between two The operation of the PMM Piezo Impact Driver Unit {MB-U) set by conditions A and B can be selected using the operation unit or foot switch. Therefore, you can switch between conditions A ‘and B depending on the condition of the membrane being manipulated. High operability, Useful general purposes If you were using conventional 3-dimensional of the PMM unit’s superior functionality injection holder. Changing the Joint Unit You can change the joint unit depending on type of three dimensional micro-manipulator on which the Piezo Impact Drive Unit (MB-U) is mounted, Please contact PRIME TECH LTD. office or one of your local distributor. Shioping charge and expenses for specification changes shall be borne by the purchaser except vwhen a new purchase order is placed Note that product may differ from the specifications, photographs, or drawings provided in the r9 manipulators, you could easily taken advantage ‘0 impact drive unit {MB-U} include specialPRIME TECH.LTD. 4. System Configuration The SYSTEM PMM-I50FU consists of the following items. Please check that all the items are contained, and inform us within one week if any component is damaged or missing. PMM Series SYSTEM [PMM-150FU] configuration Piezo impact Micro Manipulator [PMM=150FU] ‘0707-0s'_ (1) PMM Controller «PMAS-CT150) (quantity) (1-1) Controller main unit 1 (1-2) Operation box [OP-15] 1 (1-8) Foot switch [OP-16] 1 Accessories (1-a) Power cord 1 (1-b) Fuse (T3.15A, 250V) 1 (1-0) Fuse (THA, 250V) 1 (2) One Dimensional Piezo Impact Drive Unit «MB-U) (quantity) (2-1) Siding unit 1 (2-2) Impact unit 1 (2-3) Injection holder 1 (3) Accessories Fig. 2Piezo Impact Drive Unit {MB-U} (quantity) (3-1) Luer tip connector 1 for 2 nn Teflon tube) (3-2) O-ring for injection holder 4 (3-3) Cleaning shaft 1 (3-4) Hexagonal wrench (25 mm) 1 (quantity) (4) Instruction manual 1 (8) Option “For Narishige manipulator ‘AD-NP “For Leica manipulator ‘AD-LBK For Narishige manipulator ‘AD-NSL “For Eppendorf manipulator Screw(M3, 25 or 30mm) “Tubing Set for Eppendorf CellTram OcT-EPC If anything is unclear, please contact PRIME TECH LTD. or your local distributor._PRIME TECH.LTD. _ Piezo impact Mioro Manipulator [PMM-150FU} ‘0707-05° 5. Name and Function of Each Component (1) PMM Controller «PMAS-CT150) ‘The PMM controller controls the operation of the {MB-U) connected to the controller and incorporates @ power supply and controls. This allows remote controlled operation of the(MB-U) from the operation box {OP-15). Since most settings are controlled via the (OP-15) operation box, (1-1-A)_ Front Panel | PMAS=CTT60 vier wie wmnarvee co 1 ay PME TECH Fig. Front panel 1. Main power indication lamp.PRIME TECH.LTD. Piezo impact Micro Manipulator [PMM=150FU] ‘0707-05 (1-1-B) Rear Panel Fig, Rear panel 4. Main power switch 2. Power socket 3. Cooling fan 4. [0P.80x] Socket for connecting operation box {OP-15) 5. [FOOT SW] _ : Socket for connecting foot switch (OP-16) 6. [FUSE]DC — : Fuse holder DC1 (T1A, 250V) 7. 8. [FUSE] Fuse holder AC (T3.15A, 250V) [OUTPUT] _: Socket for connecting the {MB-U) Voltage output 24V max. 9. (DIR) Traveling direction selection switch © To avoid an electric shock, wi for five minutes or more after you turn off the main power switch before unplugging the {MB-U}, operation box, and foot switch connectors. '@ Never open controller cover whether the controller is switched on or off. Risk of electric shock, © If the controller (PMAS-CT150) surface is splashed with any liquid, turn off the main power ‘switch and unplug the power cord. Then wipe off the surface using a soft dry cloth or paper. If any liquid penetrates the controller, immediately turn off the main power switch and unplug the power cord. In the event of such spillage, do not use the controller (PMAS-CT150), please contact PRIME TECH LTD. or your local distributor.PRIME TECH.LTD. _ _ Piezo impact Micro Manipulator [PMM~150FU] ‘0707-05 (1-2) Operation Box (OP-15) 1.AB 2A 3. SPEEDA 4.INTA Fig.5. Unit OP=15 This switch selects the operation of the (MB-U) from the two setting conditions (A ‘and B) which you have pre-set. Condition A and B can alternately switch back and forth between A (green indication lamp) or B (yellow indication lamp). Switching to condition A results in a single beep sound, and switching to condition B results in two beep sounds. Condition A operation indicator lamp (green). If the indictor lamp [A] is lit, the (MB-U) is operating under condition A, Speed-setting dial, Set to one of 16 levels of one pulse mode (Setting the value at “1") and increasing Frequency Power 2 (slow) to 16 (fast). When the indicator lamp A lit, the operating speed of the (MB-U) is operating using the pre-set Frequency selected in condition A. Setting the value at “1” causes one-pulse mode to be entered. Operate the operation box or the movement pedal to drive the Piezo Impact Drive Unit KMB-U} by one pulse. Setting the value between 2-16 will change the pulse interval accordingly. We recommend that you select the lowest Frequency that will produce the desired results, Intensity-setting dial. Set to one of 16 levels of power (intensity) from tweak) to 16(strong). When the indicator lamp [A] is lt, the operating intensity of the (MB-U) is operating using the pre-set operating intensity selected in condition A. Increased in— tensity values are small, it allows the optimum to be obtained. We recommend that extraneous movement of the pipette/needle be minimizes by selecting the lowest intensity to complete the task.PRIME TECHLTD._ 5.B 6. SPEED B 7.INTB 8&9. Caution’ Piezo impact Mioro Manipulator [PMM-180FU] ‘0707-05° Condition B operation indicator lamp (yellow). If the indicator lamp [B] is lit, the {MB-U) is operating under condition B. Speed selecting dial. Set to one of 16 levels of one pulse mode (Setting the value at “1") and increasing Frequency Power 2 (slow) to 16 (fast). When indicator lamp B lit, the operating speed of the (MB-U) is operating using the pre-set Frequency Selected in Condition B. Setting the value at “1” causes one-pulse mode to be entered. Operate the operation box or the movement pedal to drive the Piezo Impact Drive Unit {MB-U} by one pulse. Setting the value between 2-16 will change the pulse interval accordingly. We recommend that you select the lowest Frequency that will produce the desired results. We recommended that you select the lowest frequency that will produce the desired results. Intensity setting dial. Set to one of 16 leveles of power (intensity) from 1(weak) to 16(strong). When the indicator lamp [B] is lit, the operating intensity of the {MB-U) is operating intensity the pre-set operating intensity selected in condition B. Increased intensity value are small, it allows the optimum to be obtained. We recommend that ‘extraneous movement of the pipette/needle be minimizes by selecting the lowest intensity to complete the task Arrow keys indicate how the unit may be preset to move the (MB-U) to the left (x) or right Com), © Never disassemble the operation box {OP-15) and the foot switch (OP~16). If you may get an electric shock, © IF the operation box (OP-15) and the foot switch {OP-16) surface is splashed with oil, water, or chemicals, turn off the main power switch and unplug the power cord. Then wipe off the surface using @ soft dry cloth or paper. Ff oil, water, or some chemical has penetrated the operation box {OP-15) and the foot switch (OP-16), immediately turn off the main power switch and unplug the power cord, Do not use them under such conditions. Contact PRIME TECH LTD. or your local distributor. I)__PRIME TECHLTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05! (1-8) Foot Switch _(OP-16) Fig6 Unit OP-16 1. Movement pedal Constant depressions of the left pedal results is activator of the unit according to the pre~settings in the selected channel and for the duration of pedal depression. Release of the pedal causes the unit to stop. 2. Switching pedal The condition selection pedal toggles between pre-set conditions A and B and is operated by depressing the pedal once, Note that the condition selected by the foot-pedal is automatically displayed by the selection lamp A (green) or 8 (yellow). Switching to condition A results in a single beep sound, and switching to condition B results in two beep sounds, © Never disassemble the operation box (OP-15) and the foot switch (OP=16). If you may get an electric shock, © If the operation box {OP-15} and the foot switch {OP-16) surface is splashed with cil, water, or chemicals, turn off the main power switch and unplug the power cord. Then wipe off the surface using a soft dry cloth or paper. If oil, water, or some chemical has penetrated the operation box {OP-15) and the foot switch (OP-16), immediately turn off the main power switch and unplug the power cord. Do not use them under such conditions. Contact PRIME TECH LTD. o your local distributor.PRIME TECH.LTD, Piezo impact Micro Manipulator [PMM-~150FU] ‘0707-05'_ (2) One Dimensional Piezo Impact Drive Unit {MB-U) 1. Impact unit. 2. Support adjustment knob. 3. Sliding unit. 4, Support leaf spring. 5. Injection holder, 6. Luer tip connector. Fig.7, PMM Piezo Impact Drive Unit (MB—U) ‘This is the PMM piezo electric drive unit. It translates the piezo-electric effect into movement of the device. ‘Adjusts the tension of support leaf spring on the injection holder, which is set in the V-shaped groove of holders which possess them. This is a fixing unit to support the mounted injection holder and impact unit, Holds the injection holder in position while allowing it to move when the PMM is operated, ‘This is @ special injection holder for PMM impact drive unit (MB-U). This connects with the injector line. This is only for g 2mm Teflon tube. Refer since P.16 for the installation adaptor of other types, 7PRIME TECH.LTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0107-05 Please Note} © The piezo-electric element is made of the ceramic. Do not drop, and do not throw the impact unit hard, ‘\Please Noted © The injection holder and the luer tip connector have not been sterilized. If you sterilize these ‘components, avoid dry sterilization or autoclaving. Heat may stress these components, leading to malfunctions © The luer tip connector is only for 2 am Teflon tube. If your injector line is not 2am Teflon tube, please contact PRIME TECH LTD. © The {MB-U) unit incorporates a special injection holder. It is incompatible with other type of injection holder. © Never open the case of (MB-U). If you may get an electric shock, © If the {MB-U) surface is splashed with oil, water, or chemicals, tum off the main power switch and unplug the power cord. Then wipe off the surface using a soft dry cloth or paper. If ol, water, or some chemical has penetrated the (MB-U), immediately turn off the main power switch and unplug the power cord. Do not use the (MB-U} under such conditions. Please contact PRIME ‘TECH LTD. or your local distributor.PRIME TECH.LTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05"_ 6. Installation (1) Ing ing the controller (PMAS-CT150) + After ensuring that the power switch of the main unit is turned off, connect the assembly to a power source as follows. 1) Plug the operation box {OP-15) connections into the rear-panel connector labeled OP. BOX and tighten the fixing screws. 2) Plug the (MB-U) connections into the rear-panel connector labeled OUT PUT. 3) Plug the foot switch (OP-16) connections into the rear-panel connector labeled FOOT SW. 4) Plug one end of the power cord into the power supply socket and the other end into the main outlet. [A\Please Note| © Place the controller on a horizontal table in a cool location having low levels of atmospheric dust, because it uses a cooling fan to codl its interior. ‘© For ventilation, avoid blocking the vent slit in the controller’ s bottom surface and leave a gap of at least 100mm between the rear panel and the wall Caution! © Be sure to connect the ground the power cord properly Use a commercially available power cord that the conforms to the relevant national standards. @ Do not attempt to connect the unit to a mains power supply until you have ensured that the ower switch turned off. The power switch should not be turned on until you have completed all connections, (2) Installing the PMM Piezo Impact Drive Unit (MB-U) Before installing ‘© Check that there is no backlash in the 3-dimensional micromanipulator (to which the unit, (MB-U) is going to be fitted). Tighten the fixing sorews as necessary, ‘© Check that there is no backlash in the injection holder fixing screw. [ sete up the Piezo Impact Drive Unit (MB-U) to €@ Narishige micro-manipulator.(AD-NP) eee eee Setting up the Piezo Impact Drive Unit {MB-U) to | wanipulator (AD-NSL) 2 Narishige Setting up the Piezo Impact Drive Unit «MB-U) to — see @ ASTP or AMB000 micro-manipulator. aaa Setting up the Piezo Impact Drive Unit MB-U) to — See a Leica micro~manipulator(AD-LBK) eee —> See page 18 ISPRIME TECHTD. Piezo impact Micro Manipulator [PMM~150FU] ‘uro7-o51 PHM (8-0) Setting up the (MB-U) unit to manipulator like the one made by Narishige (microscope column-fitting type), Ex1) Mounting arrangement for AD-NP (Right installation) 1) Set the coarse manipulation unit on the center of the each moving range, 2) The universal joint or the ball joint of Narishige micromanipulator is detached. Attach the sliding Unit with the joint unit (AD-NE) to the 3~dimensional manipulation unit The tilt angle degree of the slide unit is coordinated to about 10° , when the injection pipette of straight type is used. The tit angle degree of the slide unit is coordinated to become about 20° according to the bend angle of the pipette (The bend angle of the pipette once for the injection pipette of 20° ) The length of the shaft of mounting adaptor (AD-NE) is selected according to Narishige ‘micromanipulator, and the installation position is coordinated, Installation A Installation B 1 Microscope interior side 1 1 Microscope interior side 1 ~— a om | Microscope forward side | I Microscope forward side | A ae ae = ‘J About 20" (The bond angle ofthe pipette once) ‘Livove 20" (Th bend aru of the pate onc) Fig8. Direction and angle of installation 16PRIME TECH.LTD, Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05° 3) The tube from the injector is firmly connected with the injection holder, Luer tip connector Fig9 Connection of luer tip connector 4) Mount the impact unit so that the gap between the impact unit and the sliding unit is Smm. The strength of the support leaf spring is controlled by screwing the knob fully and 1g about 360" to give a standard pressure. From this pressure, the adjustment range Fig.10 Positioning of the sliding unit and the impact unit. IFPRIME TECH.LTD. : Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05 Ex2) Mounting arrangement for AD-NSL 1) Set the course manipulation unit on the center of the each moving range. 2) The slide base is installed in AD-NSL. Fig.11 Direction of installation of AD-NSL. 3) The universal joint or the ball joint of Nerishige micromanipulator is detached. Attach the sliding unit with the joint unit (AD-NSL) to the 3-dimensional manipulation unit, 4) The adjustment of the inclination angle degree loosens and coordinates the screw of A and B in two places. Fig.12 Adjustment of inclination angle degree in AD-NSL 16PRIME TECHLTD. Piezo impact Micro Manipulator [PMM~150FU] 0707-05". Ex3) Example of installing Piezo impact drive unit (MB-U) in ASTP and AM6000 of Eppendorf and Leica 1) The pipette holder installation part of the X-axis unit of Eppendorf micromanipulator is detached from the main body of the micromanipulator. 2) The plate © and the screw to fix the pipette holder are extracted from the X-axis unit, The + sorew that fixes the angle regulator on the other side of the X-axis unit is detached at the t @® + screw —~f oe ‘Screw to fix the pipette holder Fig.13Resolution of Eppendorf micromanipulator X-axis unit 3) The slide unit of PMM is matched to the angle regulator, and the screw (M3, 25mm) of the attachment is inserted from the other side of the X unit and the slide unit is fixed. M3, 25mm Serew ro it Fig.14 Method of installing the slide unit in Eppendorf micromanipulator \ 4) The adjustment of the inclination angle degree of the piezo impact drive unit is possible with ‘an angle regulator of Eppendorf. 5) The luer tip connector is connected with injection holder,__PRIME TECHLTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-0 14) Method of installation on micromanipulator M of Leica 1) The ball joint installed in micromanipulator M of Leica is removed. 2) The Mounting adaptor AD-LBK js installed in manipulator M of Leica. The main body of AD-LBK is firmly fixed with the knurling tool screw. Main body of AD-LBK Ball joint a Knurling tool screw Fig.15 Method of the mounting adaptor AD-LBK for micromanipulator M of Leica, 3) The inclination angle degree of the slide unit is coordinated by the ball joint of mounting adaptor AD-LBK. Fig.16 Inclination angle degree adjustment in AD-LBK 4) The luer tip connector is connected with injection holder. LoPRIME TECH.LTD. Piezo impact Micro Manipulator [PMM~150FU] ‘0707-08 [A\Please Note! © The (MB-U) is a precision instrument and should be handled with care. [Important] @ Any cracks and/or bends in injection holder may cause the {MB-U) to malfunction © The luer connector can only used with a Teflon tube of 2 nm outer diameter. © In fixing the joint units of the Piezo Impact Drive Unit (MB-U) to the three-dimensional manipulator, firmly tighten the joint unit fixing screw, injection holder fixing screw, and three-dimensional micromanipulator fixing screw. © Attach the Teflon tube and cable loosely enough to prevent them from becoming caught in the three-dimensional micro-manipulator or other peripheral equipment when the Piezo Impact Drive Unit (MB-U) is mounted on the manipulator.PRIME TECHLTD, Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05° ‘Separating the Impact Unit from Exclusive Injection holder| If the injection holder and the luer tip connector are washed and sterilized before mounting the Piezo Impact Drive Unit {MB-U}, follow the procedure below to separate the impact unit from the injection holder. /\Please Noted © The (MB-U) is @ precision instrument and should be handle with care, © Avoid autoclaving or dry-sterilizing the injection holder. Even slight deformation of the injection holder caused by heat may result in malfunctioning of the PMM. After washing the injection holder, dry it at 50°C or lower over an extended period, Important © Be careful not to damage the injection holder; a damaged injection holder may cause malfunctions. (Separation Method) 1) Remove the clamping screw from the injection holder. Holder Clamp O-ring Injection holder Fig.17 Inside of the injection holder 2) Loosen the injection holder fixing screw of the impact unit and pull the injection holder from the impact unit, working from the side to which the luer tip connector is attached. Injection holder fixing serew luer tip connector Injection holder Za Impact unit Fig.18 Separate of impact unit and injection holder LePRIME TECH.LTD. 7. Method of Use Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05 {Preparation} Before operating the PMM, first make sure that the main power switch of the controller is tured off then follow the following procedure, (1) Check whether the outlet for the connection cord from the Piezo Impact Drive Unit (MB-U) is firmly fixed, whether the cord is damaged, and whether the connection cord to the controller is loose 3KIF any problems are found, please contact PRIME TECH LTD. or your local distributor, (2) Mount the injection holder so that the gap between the impact unit and the sliding unit is about 5mm, LXcaution © If the connection cord or connector to the use it; you may get an electric shock. “ezo Impact Drive Unit (MB-U) is abnormal, do not Important! © The system may become defective if the Piezo Impact Drive Unit (MB-U) is driven while the sliding unit contacts the impact unit. Be sure to set the gap between these units at about Smrn, {Main Operations) (1) Turn on the power switch. The power indicator lamp will light up. (2) Use SPEED A, INT A, SPEED B, INT B to set the {MB-U) and the conditions required for manipulation (8) Use A/B on the operation box or Right pedal of foot switch to select condition that you need. ‘Switching from condition A to condition B produces one beep sound; switching from condition B to condition A produces two beep sounds. 3K When you change the condition you have to confirm the indication lamp on the operation box is changed, (4) You can use the [tl], [BB] keys on the operation box to move the mounted needle forward or backward. 2k The foot switch is a leftward pedal that can be operated only in a leftward direction, Adjust this switch only after reading the “Adjustment Procedures” section that follows, and observing all instructions contained therein, (5) When you have finished a session, turn off the main power switch on the operation box. 2X Wait for five minutes or more after you turn off the main power switch before you turn on the main power switch,PRIME TECH.LTD. - Piezo impact Micro Manipulator [PMM-150FU} ‘0707-05! (Check and Adjustment Procedures) (1) Check whether the condition can be switched using either the [A/B] switch on the operation box or the [A/B] switch pedal of the foot switch. (2) Use the SPEED A, INT A, SPEED B, INT B to regulate the movement of the Impact Drive Unit «MB-U}. + Setting SPEED A or B sets the speed of the Piezo Impact Drive Unit {MB-U). Normally, set this speed at level 10 or below (a minimum req Setting SPEED A or B at “1” causes a one-pulse mode to be entered. In this mode, pressing the [ad] or [9] key instantaneously drives the Piezo Impact Drive Unit (MB-U) by one pulse. + INT A or B: Sets the operational intensity. d value), Set this dial at @ minimum required value unless the operation requires intensity due to the hardness or elasticity of cell membranes. Setting this dial at higher value causes operational speed to increase slightly. If any micro-instrument begins to be vibrated, set this dial at a smaller value, Normally, it should be set to level 10 or below (a minimum required value). * Setting the value of SPEED A or B at “1” drives the Piezo Impact Drive Unit {MB-U) by one Pulse when the [eal] or [im] keys of the operation box or the drive pedal of the foot switch is pressed. Note that to keep pressing the [=s@l] or [BE] keys or the drive pedal dose not drive the «MB-U). To drive the (MB-U) continuously in one-pulse mode, you must repeat to push the [==] or [me] keys or press the drive pedal [DIR] Switch] [DIR] can switch the driving direction of the Impact Drive Unit (MB-U). Mounting the Piezo Impact Drive Unit (MB-U) (01) Three-dimensional manipulator on the right-hand operation side R Three-dimensional manipulator on the left-hand operation side L [iease Notel © Before switching [DIR]. be sure to turn off the main power switch. For information on problems occurring during operation, please see “Trouble Shooting”. you have any questions or comments, please contact PRIME TECH LTD. or your local distributor.PRIME TECHLTD. 8. Trouble-shooting Controller (PMAS-CT150) Piezo impact Mi ioro Mi lator [PMM=150EU] ‘0707-05' Sua ‘Cause ‘Countermeasures No power: Loosely plug the power cord into the receptacle, Be sure the source outline is supplying power. Correctly connect the power plug to the outlet at the back of the controller. Replace the blown fuse with a good one of the proper size. (Refer P. 29) If the fuse blows frequently, contact your local distributor. Operation box (OP-15) = Foot switch (OP-16) Situation Cause Countermeasures Operating the operation box or foot switch dose not drive the Piezo Impact Drive Unit (MB-U). The connector between the controller and the operation box, foot switch, or Piezo Impact Drive Unit (MB-U) is loose or disconnected. Be sure to turn the main power switch off; after waiting for at least five minutes, connect the connector correctly. (Refer P. 9) Pressing the rightward or leftward movement key or the drive pedal dose not drive the Piezo Impact Drive Unit (MB-U) (the connectors are connected correctly), mode. The SPEED for the selected condition has been set at “1” in order to initiate one-pulse While in one-pulse mode, pressing the rightward or leftward movement key or the drive pedal instantaneously drives the Piezo Impact Drive Unit by one pulse. Increase the set value of SPEED to check whether the Impact Drive Unit {MB-U > is being driven. (Refer P. 25) If this action dose not drive the Impact Drive Unit {MB-U}, please contact PRIME TECH LTD. or your local distributor. No sound when condition A/B switched, Be in silent mode. Turn the main power switch off, after waiting at least five minutes, ‘turn it on while pressing both the rightward and leftward movement keys. The driving direction set by the rightward or leftward movement key is opposite to the actual driving direction of the Impact [Drive Unit (MB-U). direction, DIR has been set to opposite Turn the main power switch off, after waiting at least five minutes, switch DIR. Then, turn the power supply on again, (Refer P. 25) 25PRIME TECH.LTD. Piezo Impact Drive Unit {MB-U) Piezo impact Micro Manipulator [PMM~150FU] ‘ov07-0s! ‘Situation Cause Countermeasures: Power is on, but the {MB-U) dose not respond correctly. (MB-U) secured The control box €OP-15), foot switch (OP-16), and connector are not to the PMM controller (PMAS-150). Correctly secure control __ box {OP-15), foot switch (OP-16), and (MB-U) connector to the outlet on the back of the PMM controller {PMAS-150). (Refer P. 19) sional There is play in the 3-dimen— manipulator that has been mounted. Tighten it sufficiently to fix it. The injection screw has become loose. holder fixing Tighten it firmly so that it will not move. (Refer P. 13) The joint unit fixing screw has become loose. Tighten it firmly so that it will not move. (Refer P. 13) Piezoelectric been damaged elements have Contact us or your local distributor. The motion of the injection holder is unstable. values, INT are set to excessively large Set the dial to the lowest intensity possible, The end ofthe micro-capillary vibrates. ‘SPEED are set to excessively large values. Set the dial to the lowest speed possible, holder is loosely fitted. The micro-capillary isnot | After confirming that silicone tube correctly fixed into the | has been fitted properly, insert a injection holder. micro-capillary of a sufficient length and tighten up. Silicone tube in injection | Tighten up. If the silicone tube is old, replace it. (Refer P. 29) There is backlash in the 3 dimensional manipulator that has been mounted. Tighten it sufficiently to fix it The injection holder screw has become loose. fixing Tighten it firmly so that it will not move. The joint shaft fixing screw has become loose. Tighten it firmly so that it will not move. loosed. Force of support leaf spring is Tighten support adjustment knob firmly. If it proves impossible to tighten it, please contact us or your local distributor. Foreign matter has gotten into the sliding groove, Remove the injection holder integrated with the impact unit from the sliding unit. Remove any foreign matter from the V-shaped groove and presser spring. face. Foreign matter has gotten i the sliding groove causing nicks and wear on the sliding Dismantling and readjustment are required. Contact us or your local distributor.Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05" Operation is erratic at the start, The V-shaped groove in the sliding unit is dusty or dose not allow sufficient sliding. Set SPEED and INT to higher values and drive the PMM back to forth. Once the movement has become stable, set the values back to their predetermine levels, ‘The PMM is being driven while the sliding unit contacts the impact unit. Set the gap between the sliding unit and the impact unit at about 5mm. Movement to either the right or left is slower than normal. It moves either The (MB-U) is installed at steeper angle than normal Install The (MB-U) at an angle of about 10° only right or left The (MB-U) power cable or injection tube are caught on the 3-dimensional micro ma~ nipulator or other peripheral machinery. Provide slack in the cable and line ‘supplying the (MB~U). The (MB-U) has be driving under the impact unit touch sliding unit. Keep about Smm between impact unit and slide unit, If you have any questions about your unit, please contact us or your local distributor.PRIME TECH.LTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-08' 9. MAINTENANCE Replacing Consumable Parts] «Replacement of fuses) To replace the fuse, follow the procedures below. [AXcaution' © Unplug the power cord before removing the fuse. If the cord is left plugged in, you may receive an electric shock © Always use the spare fuse provided or an equivalent that conforms to the same standards. (1) Disconnect from power supply. (2) From the rear of the controller, detach the fuse holder housing the fuse to be replaced, turning the holder left. (3) Remove the defect fuse from fuse holder and replace it with a new one, (4) Set the fuse holder back in place, turning it to the right and screwing it completely onto the controller If the fuse blows again after replacement, contact our company or one of our distributors, «Replacement of silicon tube) AAs the silicone tube in the injection holder ages, the micro-pipette may become loose, and the liquid in the injector may leak. If you identify such a problem, it is time to replace the silicone tube, For replacement, follow the procedures below. (1) Remove the injection holder coupled with the impact unit main body from the slide unit of the Piezo Impact Drive Unit {MB-U). (2) Remove the clamp screw of the injection holder. From the interior of the injection holder, remove the spacer and the silicone tube. If the silicone tube is dificult to remove, use the cleaning shaft provided as an aid, (3) Set the attached silicone tube piece in place in the injection holder and put the spacer on it. (4) Tighten the clamp screw. This completes the replacement of the silicone tube. [Himportant] © IF the silicone tube in the injection holder is too short, the pipette cannot be fastened firmly Make sure the tube length is sufficient © When replacing the silicone tube and cleaning the injection holder, take care not to lose the spacer removed from the interior of the injection holder and the clamp screw.__PRIME TECHLLTD. f Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05 Cleaning of PMM-150FU PMM CONTROLLER (PMAS-CT150), OPERATION BOX(OP-15) and FOOT SWITCH(OP-16) Remove any dirt on the surfaces with a soft cloth that has been wrung out after soaking in alcohol ‘oF neutral detergent. Do not use acetone, a diluted solution of a nitro compound, or the like. Take care not to damage the coating or plastic surfaces. © Never disassemble the controller, operation box and foot switch. If you may get an electric shock. © Before starting cleaning, check that the power switch is turned off and be sure to unplug the power cord Piezo Impact Drive Unit (MB-U) + Remove any dirt on the impact unit main body, sliding unit, and injection holder with a soft cloth that has been wrung out after soaking in alcohol or neutral detergent. When cleaning the V-groove on the slide unit, use a cotton swab soaking in alcohol or neutral detergent or acetone. After cleaning, dry the unit thoroughly. If a broken micropipette has fallen into the injection holder, first remove the lure tip connector and clamp screw. Then, using the provided cleaning shaft, push the glass fragment out of the holder, To clean the injection holder, first separate it from the impact unit main body. Immerse the holder inva diluted neutral detergent. Then thoroughly rinse the holder with water and dry it. [A\ Caution’ © Never disassemble the impact unit main body. If you may get an electric shock. © Before starting cleaning, check that the power switch is turned off and be sure to unplug the power cord {Umportani © Take care not to damage the surface of the injection holder. © To dry the injection holder, allow it to stand at room temperature or at 50°C or lower for as long as necessary. [Gansomabia Par] When ordering replacements for consumable and lost parts, specify their part numbers as shown below. NAME CAT. te, STANDARDS Spare fuse OF-ADIS Tae | Tubing Set for H-8/8 | OCT-SF10 ere cae Holder Grip set ooR-5 pce os : Please note that the shipping cost for any product in the list above should be borne by the customer. aPRIME TECHLTD. Piezo impact Mioro Manipulator [PMM-150FU] ‘0707-051 10. Limited Warranty PRIME TECH LTD. limits the warranty on this instrument to repair or replacement of defective components for one year after the date of shipment, provided the instrument has been operated in accordance with the instructions outlined in the instruction manual except the MB-U. We limits the warranty one month after the date of shipping date on piezo-elements in ‘the MB-U. (The MB-U unit is assembled with Piezoelectric elements. It is very fragile because it is made of ceramic parts. Please avoid shock or dropping to it and pay full attention when you handle it) ‘Abuse, misuse or unauthorized repairs will void this warranty. Limited warranty work will be performed only at the factory, the cost of shipment both ways to be borne by the user. The PMM can be used for research only, Do not be used for cli | surgery,PRIME TECH.LTD. Piezo impact Mioro Manip lator [PMM-150FU] ‘0707-05__PRIME TECHLTD. Piezo impact Micro Manipulator [PMM-150FU] ‘0707-05! PRIME TECH emm Piezo impact Micro Manipulator one et a, FItbF 9 RE BI PRIME TECH LTD. 635NAKAMUKAIHARA, TSUCHIURA-SHI, IBARAKI-KEN, 300-0841 JAPAN Phone +81-20-890-4517 Fax 481-29-830-4515, E-mail: pmm@primetech-ip.com Web site: http://ww.primetech com 3?