Silk Fibroin (SF) and Alginate (ALG) are biomaterials that have therapeutic effect in treatment of wounds dressing which are hemostatic and the ability upon absorption of wound exudates. This
research aims to make delivery model for wounds dressing in the form of fiber membrane results from polyelectrolyte complexation (PEC) through coacervation method. SF from silkworm
cocoons (Bombyx mori Linn) used as polycations, and ALG as polyanions agent. Characterization of PEC fiber membranes are conducted with analysis of the functional groups (FTIR), crystallinity
degree (XRD), thermal properties (DSC), microscopic (SEM). Then, the study continued to the measurement of coacervation yield. SDS PAGE was used to verify the protein. This research has
obtained SF with better characteristics that suitable for the treatment of skin wounds, which is responsive to the pH environment. The membrane is a modified form from the hydrogel-forming
of SF/ALG coacervate. Characterization analysis of the membrane shown that the electrostatic bonds occurs between SF with ALG (PEC) and approved that transformation polymorphic forms of
SF from Silk I (metastable) to Silk II (stable) was happened. Polymorphism, thermal properties, and crystallinity degree of SF can be modulated and controlled to obtain the appropiate final
result of the membrane.
Keywords: Silk fibroin, sodium alginate, coacervation, polyelectrolyte complex, fiber membrane
Step 01
Silk degumming Formulation
Step 05 Step 02 F1 = SF/ALG (1:1); F2 = SF/ALG (1:3)
PEC fiber membrane SF solution (*RSF 1) F3 = SF/ALG (3:1)
Fibroin protein
Cocoons weight = 25,051 g
Methods
SDS PAGE analysis Hydrogel-forming of SF/ALG coacervate SF/ALG PEC fiber membrane
Discussion
Result &
Fourier Transformed Infrared (FTIR) X-Ray Diffraction (XRD) Differential Scanning Calorimetry (DSC) Coacervation yield
(a) Degummed silk; (b) SF paste; (c) ALG SF/ALG PEC fiber membrane (a) F1 (1:1);
(b) F2 (1:3); (c) F3 (3:1)
(References of SF IR-band: Pelton, 2000; Barth, 2002; Garidel, 2006; Hu, 2006; Lu, 2010 | SF difractogram: Shimizu, 1941; Hirabayashi, 1967; Magoshi, 1977; Ayub, 1994 | SF DSC: Sashina et al., 2007)
th
Publish at International Seminar In Pharmaceutical Technology 27-28 January 2016, Bandung, West Java, Indonesia