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QuickComb™ Kit

for Bulk Soybeans


Catalog Number AS 076 TC
Highlights:
 Results in 5 minutes or less Intended Use
 Convenient comb format This EnviroLogix QuickComb Kit for Bulk Soybeans is designed to extract and
 One Common Extraction™: detect the presence of certain proteins at the levels typically expressed in genetically
- Roundup Ready® modified soybeans. The QuickComb may contain the following QuickStix™:
- DMO/RR2 Xtend®
- LibertyLink® Protein/Trade Name Sensitivity
CP4 EPSPS / Roundup Ready 1 soybean in 1000 (0.1%)
DMO / Roundup Ready 2 Xtend 1 soybean in 400 (0.25%)
Contents of Kit: PAT/pat / LibertyLink 1 soybean in 200 (0.5%)
 100 QuickCombs, packaged
5 combs per foil pouch
 Sample cups and disposable
How the Test Works
transfer pipettes In order to detect the proteins expressed by genetically modified bulk grain, the
sample must first be extracted to solubilize the protein. All QuickStix included in the
QuickComb are specially formulated to detect their analytes in a Common Extraction.
Items Not Provided:
Each QuickStix Strip has an absorbent pad at each end. The protective tape with the
 Blender for sample prep:
arrow indicates the end of the strips to insert into the reaction cup. The sample will
1. Oster® Sunbeam blender
travel up the membrane strips and be absorbed into the larger pad at the top of the
model #4094 or equivalent
(with 4 oz. polystyrene strip. The portion of the strip between the protective tape and the absorbent pad at the
blender jar, ice crusher top of the strip is used to view the reactions as described under “Interpreting the
Results.” Please avoid bending the comb.
blade, gasket, and blender
base) ~or~
2. Waring blender model Sample Preparation
31BL91 or equivalent (with
Step 1: Determine Number and Size of Sub-samples
glass Mason jars and jar
adapter [Eberbach #E8495] 1. Collect a composite sample according to USDA/GIPSA instructions found in the
along with protective cover reference documents listed in the margin on Page 2.
~or~ 2. The following is another helpful reference for use in designing a sampling plan:
3. BUNN coffee grinder Remund, K.M., Dixon, D.A., Wright D.L., Holden, L.R. “Statistical
(industrial style grinder considerations in seed purity testing for transgenic traits”, Seed Science
set on AutoDrip setting) Research, June 2001, Vol. 11 No.2, pp. 101-119.
 Graduated cylinder 3. To select the appropriate sample size, determine the purity standard and the
 Tap water degree of confidence required. Confidence level means the statistical probability
 Protective cover for blender that the % of GMO soybeans in the lot is below the selected purity standard.
jar while grinding This calculation should be done for each trait tested, then choose the largest sub-
sample volume result.
Step 2: Determine Sub-sample Weight, Jar Size, Grind Times and
Water Volume Needed for Sample Preparation
For sampling scenarios at different 1. Determine the average weight of the grain from the lot to be tested. Count and
screening or confidence levels,
weigh 100 kernels/seeds, then divide by 100.
refer to the USDA/GIPSA Excel
spreadsheet described below, or 2. Calculate the sub-sample weight (g) needed for testing, (number of seeds X
call EnviroLogix Technical average seed weight).
Support for assistance. 3. Calculate water volume needed for sample preparation. The Soybean Common
Extraction uses a water volume to sample weight ratio of 5 to 1.
Example Calculation using a 100 kernel sub-sample with an average kernel weight of 0.15g.
0.15g x 100 =15 g x 5 mL = 75 mL water for extraction

Rev. 03-22-17
QuickComb Kit for Bulk Soybeans
Page 2 of 4

4. Choose an appropriate jar size and grind time based on the type of blender
USDA References: available for sub-sample preparation (see Table 2). Oster Sunbeam Blender with
 www.gipsa.usda.gov/fgis/ ice crusher blade is recommended over the Waring Blender for its bean grinding
handbook/gihbk1_inspec.aspx efficiency. (Note that bean grind time is longer and requires additional steps*
- USDA Grain Inspection Handbook, when using a Waring Blender). The grind quality should be such that 40-50%
Book 1, Grain Sampling.
passes through a 20-mesh sieve.
 www.gipsa.usda.gov/fgis/
biotech/sample2.htm - Guidance Table 2 - Soybeans
document entitled Sampling for the
Detection of Biotech Grains.
Grind Time
# of Beans Sub-sample on High
 www.gipsa.usda.gov/fgis/ (approximate) Blender Type weight (g) Jar size (oz.) Speed
biotech/sample1.htm - Practical Oster
Application of Sampling for the 100-200 16-38 8 20 seconds
Detection of Biotech Grains. Sunbeam
 www.gipsa.usda.gov/fgis/ 100 Waring 16-38 8 60 seconds
biotech/samplingplan1.xls - (2 X 30 sec.*)
This website provides a simple to use 60 seconds
200-400 Waring 38-65 16 (2 X 30 sec.*)
Sample Planner (29K Excel
Spreadsheet). The planner allows
you to enter different assumptions in
* For best results blend beans for ½ of total time, remove the jar and shake to redistribute
terms of sample size, number of larger particles, replace and resume grinding.
samples, acceptable quality level and
to determine the probability of Step 3: Prepare the Sample
accepting lots with given
concentration levels. It also plots the 1. Weigh sample into the appropriate vessel.
probabilities in graph form for easy
interpretation. Specific data can be 2. Put protective cover over glass jars.
saved for documentation and future
analyses. 3. Grind sample on high speed all whole beans are finely ground.
4. Add the volume of tap water calculated above (see formula above or at left).
5. Cap and shake jar vigorously for 30 seconds, insuring that the entire sample is
wet. Sample will begin to settle immediately and liquid can be drawn off at that
time. Avoid pulling up particles with the transfer pipette.
6. Transfer 12 mL of the liquid portion from above the settled sample into the
sample cup. The level should be above the bottom of the arrows but below the
Choose grinding method
top of the lower colored portion of the QuickComb strips.
7. To prevent cross-contamination, thoroughly clean blender parts and jars to
remove dust and residue prior to preparation of each sample, and use a new
Soybean Common Extraction sample cup for each. If pipetting, use a new tip or disposable pipette for each
Grams of beans x 5 = mL water sample.
For example: (100 x 0.15)=15g
15 g x 5 = 75 mL water
How to Run the QuickComb Test
Extract in tap water
1. Allow refrigerated foil pouch to come to room temperature before opening.
Remove the QuickCombs to be used. Avoid bending the strips or handling the
loose comb end. Reseal the canister immediately, if so packaged.
2. Place the comb into the three-ounce cup containing 12 mL of the liquid soybean
extract. The sample will travel up the strips.
3. Allow the comb to develop for 5 minutes before making final assay
interpretations. Positive sample results may become obvious much more quickly.
4. Remove the QuickComb from the cup to read visually. To retain the combs, cut
Transfer extract to cup, off and discard the bottom section of each strip covered by the arrow tape.
about 3 pipettefuls Note: Use extreme caution to prevent sample-to-sample cross-contamination
with grain, fluids or disposables.

Rev. 03-22-17
QuickComb Kit for Bulk Soybeans
Page 3 of 4

Interpreting the Results


Development of the Control Lines within 5
minutes indicates that the strips have
functioned properly. Any strip that does
not develop a Control Line should be
discarded and the sample re-tested using
another comb or corresponding strip.
If the extract is from a sample containing at
least the detection level of the strip’s
analyte on the QuickComb, a second line
(Test Line) will develop on the membrane
Place QuickComb in cup strip between the Control Line and the
Control Line
protective tape. The results should be
interpreted as positive for that strip’s Test Line
protein expression.
Cut here
If the extract is from a sample containing
less than the listed detection levels, the
strip will only develop a Control Line.

Kit Storage
This QuickComb Kit should be stored at
room temperature, or refrigerated for
Roundup DMO Liberty-
longer shelf life. Please note shelf life on
Ready Link
the kit box for each storage temperature.
The kit may be used in field applications; however, prolonged exposure to high
temperatures may adversely affect the test results. Important: do not open the
container (foil pouch or canister) until ready to use the combs. Allow container to
come to room temperature before opening to prevent condensation. Immediately re-
seal unused QuickCombs in the canister, if so packaged.

Precautions and Notes


 This kit is designed to be read visually as a screen for presence or absence, and is
Any clearly discernable pink no designed to be quantitative.
Test Line is considered positive  As with all tests, it is recommended that results be confirmed by an alternate
method when necessary.
 The assay has been optimized to be used with the protocol provided in the kit.
Deviation from this protocol may invalidate the results of the test.
 The results generated through the proper use of this kit reflect the condition of the
working sample directly tested. Extrapolation as to the condition of the
originating lot, from which the working sample was derived, should be based on
sound sampling procedures and statistical calculations which address random
sampling effects, non-random seed lot sampling effects and assay system
uncertainty. A negative result obtained when properly testing the working sample
does not necessarily mean the originating lot is entirely negative for the analyte or
protein in question.
 A strong positive result may safely be interpreted in as little as 2 minutes after
sample addition. It is not safe, however, to interpret negative results prior to 5
minutes.
 Protect all components from hot or cold extremes of temperature when not in use.
Do not leave in direct sunlight or in a vehicle.
 CAUTION: Tightly closed containers of soy extract, if left sitting for several
hours, may ferment and cause the lid or container to burst. Dispose of extract
when testing is complete.

Rev. 03-22-17
QuickComb Kit for Bulk Soybeans
Page 4 of 4

LIMITED WARRANTY
EnviroLogix Inc. (“EnviroLogix”) warrants the products sold hereunder (“the Products”)
against defects in materials and workmanship when used in accordance with the applicable
instructions for a period not to extend beyond a product’s printed expiration date. If the
Products do not conform to this Limited Warranty and the customer notifies EnviroLogix in
writing of such defects during the warranty period, including an offer by the customer to return
the Products to EnviroLogix for evaluation, EnviroLogix will repair or replace, at its option,
any product or part thereof that proves defective in materials or workmanship within the
warranty period.
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This Limited Warranty states the entire obligation of EnviroLogix with respect to the Products.
If any part of this Limited Warranty is determined to be void or illegal, the remainder shall
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License
EnviroLogix has developed some tests in this kit using proprietary reagents as well as reagents
licensed from Monsanto Company.

Roundup Ready and Roundup Ready 2 Xtend are registered trademarks of Monsanto
Technology, LLC
LibertyLink is a trademark of Bayer CropScience AG
EnviroLogix, the EnviroLogix logo, QuickComb, QuickStix, and Common Extraction are
trademarks of EnviroLogix Inc.

© EnviroLogix 2017

Rev. 03-22-17