BECKMAN DU*-6 UV-Visible Spectrophotometer OPERATING MANUALCONTENTS SECTION ONE 1 12 13 14 Lad 142 143 1a 15 Two 24 aa 221 222 223 224 23 24 2at 242 28 26 26.1 262 263 264 THREE six SEVEN 7 72 DESCRIPTION DESCRIPTION AND INSTALLATION Intended Use ... General Information Keyboard Description Function and Calculation Options. Single Wavelength Mode ‘Sean Mode and Derivative Function ‘Time Drive Mode . QUANT I Storage and Installation Information OPERATION . Initial Start-up Procedure... 7 Analysis Programs and Data Collection » Single Wavelength (3) Mode... Multi Wavelength ()) Mode - ‘Sean Mode «... Time Drive Mode Auto Zero Feature ata Presentation with Opt ‘Automatic Sean Plotting... 4+. Replot Feature (Optional) «....- Scan Search Festure (Optional) Storage and Recall of Data and Programs Storing a Program -.... Recalling a Stored Program . Deleting a Program Deleting a Scan... AUTOMATIC INSTRUMENT FUNCTIONS Start-up Checks Calibration Routine - Background Correction (Scan Mode Only) Automatic Blanking (Auto Zero) « DIAGNOSTIC AND ERROR MESSAGES Power-up Diagnostic Messages . Messages During Operation MAINTENANCE PROCEDURES Source Removal and Replacement . Visible (Tungsten) Lamp Replacement UV (Deuterium) Source Replacement . Source Compartment Cover Replacement Peaking Procedure ....... Peaking the Visible (Tungsten) Source: Peaking the UV (Deuterium) Source . Main Power Fuse Removal and Replacement Replacement of Fuse Bank Fuses Replacement of 220-Volt Fuse (220-Volt Instruments, Only) SUPPLIES SPECIFICATIONS General Specifications... Sample Compartment Dimensions PAGEOPERATIONAL PRECAUTIONS ‘These operational precautions are provided so that the user may avoid actions which could damage the DU®.6 ‘Spectrophotometer or adversely affect the validity of a {qualitative or quantitative determination. Hf you have purchased any additional accessories for the DU-6 Spectrophotometer, be sure to read the special Instruction sheet which was provided with the accessory for operational precautions and information. Accessory instructions complement, but do not replace the operat- ing instructions contained in this manual, and should be read thoroughly. Accessory instructions may be inserted into this binder for future reference. 1, READ BECKMAN INSTRUCTIONS 015-245324, DU-6 SPECTROPHOTOMETER OPERATING MANUAL, BEFORE OPERATING THIS INSTRUMENT. 2, Install the instrument as directed in the installation packet, and in Section One, Paragraph 1.5 of this, manual. Use only a grounded, three-prong outlet as ower source. DO NOT USE ADAPTERS. 8. Do not use the top of the unit as a work or storage ‘surface. Spilled liquids can damage the instrument. 4, Follow instrument calibration and maintenance pro- ‘cedures carefull 5. Do not touch the sources! If this should accidentally ‘occur, clean the source thoroughly with alcohol and clean cloth, 6. Do not open the sample compartment when a test is in progress. 7. Allow the instrument at least thirty minutes to warm up If thas been turned OFF for an extended timo.DESCRIPTION AND INSTALLATION 1.1 INTENDED USE This device is intended for use in qualitative or quantita- ve bioresearch or industrial procedures which require spectrophotometric measurements, It may also be used {as a general-purpose spectrophotometer for analytical ‘and investigative applications, 1.2. GENERAL INFORMATION ‘The DUS-6 Visible and DU®-6 UV-Visible Spectropho- tometers are microprocessor controlled, computing spectrophotometers which operate in the ultraviolet and visible regions of the electromagnetic spectrum. Both Instruments feature a CRT video display which provides operational prompts, and a keyboard for operator input, ‘The DU-6 Visible Spectrophotometer operates from 325 10900 nm, using a Tungsten source. The DU-6 UV-Visible Spectrophotometer incorporates an additional deuto- rlum source which extends the analytical wavelength SECTION ONE range to 180 nm. With the addition of various acces- sories, the instrument may be upgraded and customized to fit specific user needs. Operational and maintenance instructions which apply to both DU-6 Spectrophotometers are contained in this manual. Instructions which pertain to the use of acces- sories will be provided separately and are to be inserted into this binder for later reference. ‘As with any instrument, experience in spectropho- tomotric measurements, manipulation of spectral data, and a familiarization with the operational instructions will ultimately enhance the quality of the results obtained, 1.3 KEYBOARD DESCRIPTION ‘The DU-6 Spectrophotometer keyboard Is used for all operator input. Functions of all keys are given in Figure 1-1 and in the paragraphs following U6 Spectrophotomater Keyboardfanace waa » OPERATING MODE KEYS: The Operating Mode Keys are used to choose the mode for the analysis program. When depressed, the CRT will display the list of analysis parameters available for modification ‘wULTI ME STORAGE CONTROL KEYS: The STORE key is only operational when an optional storage accessory (such ‘as Quant I) has been installed. It is used to access the storage functions described in Paragraph 2.6. The srore| rePtor| REPLOT key is only operational with the Quant | or other storage accessory. It is used to access the Replot mode, Paragraph 24.2. COPY KEY: This key is only operational when an optional coe Printer/Plotter has been installed. When depressed, information displayed on the CRT will be printed, Refer also to Paragraph 2.4 anol AUTO ZERO: The AUTO ZERO key is used to reset the 2eR0 zero value during an analysis, Refer to Paragraph 2.3, {or operational details. aial SCAN SEARCH CONTROLS: These ARROW keys are Seance Used to control the optional Scan Search feature described in Paragraph 25. PROGRAM CONTROL KEYS: START key initiates instru- ment calibration. The STOP keys is used to terminate start| | stor | | aun any function already in progress, The RUN key initiates backgroundand sample readings, Refer to Section Three for complete detail. CURSOR CONTROL KEYS: These four ARROW keys are Used for manual positioning of the cursor field. Each keystroke repositions the cursor one position in the Girection of the arrow. UP and DOWN keys are also used in conjunction with the optional program storage.SELECT KEY: Used to edit bracketed parameters, Each keystroke displays a now option. Must be followed by depression of the ENTER key. NUMERIC KEYS: These keys are used for entry of nutuer- ical (nonbracketed) parameter options. All numerical seu dG ToC JEG 7) ] be ween ce entries must be followed by the ENTER key. ENTER KEY: Must follow all operator input made with ‘SELECT or NUMERIC keys, CLEAR ENTRY: Clears the entty currently displayed by the cursor. 1.4 FUNCTION AND CALCULATION OPTIONS ‘Thore are four basic operational modes available on the DU-8 Spectrophotometer: Single Wavelength, Multiwave- length, Wavelength Scan, and Time Drive. In each of these operating modes, data may be collected in either absorbance units or as % transmission. Additional calculation features which pertain only to specific oper- ational modes are discussed below. 1.4.1 SINGLE WAVELENGTH MODE In addition to absorbance and %T data collection, a concentration calculation is available in the Single Wavelength mode. (Refer also to Paragraph 2.2.1.) The operator may elect to multiply absorbance readings by a specified value using either the FACTOR or STANDARD calculation. These calculations are performed by the Instrument as follows: FACTOR Calculation: Result = (Absorbance of Sample) x (Factor) STANDARD Calculation: (Absorbance of Sample) Result = ‘Apsorbance of Standard) X (Concentration of Standard) where (Factor) and (Standard Concentration) values are centered by the user. 1.4.2 SCAN MODE AND DERIVATIVE FUNCTION In the Scan mode, there ara five scanning speeds avail- ‘able: 300, 150, 60, 30, and 15 nm per minute. When using the Scan mode (Paragraph 2.2.3) the user has the addi tional option of data collection using the first or second Gerivative of absorbance, as a function of wavelength. ‘The derivative function i useful in Wavelength scan- ning as it allows the userto extract important information rapidly from the raw data. The first derivative (1 DER) may be used to locate precisely the peaks and valleys in a spectrum. Similarly, the second derivative (2 DER) function is offen used in wavelength scanning to help visualize fine spectral structures which are super- imposed onto a broad absorption band, While this caleu- lation does not affect the inherent resolution of the data, when used judiciously, it will accentuate spectral details, allowing the user to locate fine structures. To get the mast from derivative data manipulations, however, the user should clearly understand the mathe- matics involved. A derivative (whether first or second) is calculated from the shape or “slope” of the spectral absorbance data over a short wavelength interval. This interval is called “delta lambda” (a4). Since a single data point cannot determine a line, the data immediately before and after @ given data point (i.e., within the an segment} must be examined. Since the aa is relatively short, the data points within this segment may be 3approximated to be a straight line, for which a slope value may be calculated. ‘Although the aA Segment itself is quite short in relation to the length of the entire scan, its width is very impor- tant, Furthermore, different chemical compounds may differ substantially in the widths of their important spec- tral features. Thus the exact value for \ may be chosen by the user to yield tho best results for the sample under sludy. The a parameter may be selected from the fol lowing values: 0.2 nm, 0.4 nm, 0.8 nm, 2.0 nm, 4.0 nm, 8,0.nm, or 16.0 nm. (Same values are noi compatible with some scanning speeds. Refer to Table 1.) ‘Always Koop in mind that the derivative scan will be shortened by ¥ (ai) at each extreme of the scanning range. This is because there is insufficient data within the a2 interval to calculate a derivative for these extreme: data points. ‘Similarly, a sufficient range and number of data points {scanning range) must be used in the initial scan to obtain the best possible results from the derivative cal- culation. For this reason, the minimum scanning range is 10 nm for all values of AA except 16 nm, for which the minimum is 17 nm. [oble 1, Acceptable values Soo0d ‘minimum scanning range of 10 am le aasurod for each, ‘except 160 (mummum range of 17 nm) Iran unaccenteble ‘Salve ls entered an evar message willbe csplayed AN tom) 02 04 08 20 1.4.3 TIME DRIVE MODE ‘The option of first or second derivative data collection Is. also available in the Time Drive made. Time Drive data may be collected at any of the follow- ing data rates: 1, 10, 60, 120, or 900 readings per minute. Data raie, as weil as total time of the analysis is selected during the creation of a Time Drive program. The option of first or second derivative data collection Is also available in the Time Drive mode. Because the initial scan produces absorbance data as a function of time, the first derivative Is the slope of the resultant line of rate of change of absorbance with time, This can be vory useful in tho analysis of reaction kinetics. Unlike the derivative-based calculation functions used inthe Wavelength Scanning mode, Time Drive derivatives are calculated using a set value for the interval. (Refer carefully to Paragraph 1.4.2, particularly to the informa- tion regarding derivative calculations.) However, due to the nature of the derivative calculation, a sufficient num- parameters which must be used are given in Table 2 for each of the possible data collection rates. ‘Table 2, Mima Analysis Tes for Is monerime fasten an tnd the deren calulaon wins be petorma Daas el a ve an o |] «| © | wo | 20 Minimum 5min | amin | sane | Sane | sone 1.4.4 QUANT I STORAGE AND CALCULATION OPTIONS When the DU-6 Spectrophotometer is equipped with the Quant | Storage and Calculation Accessory (Beckman Part §96148) or any accessory which includes Quant 1 Capabilities, additional calculation and display features are available. Each scan is automatically stored in the instrument memory until the next scan is run. In the Scan mode, data initially collected as Absorb- ‘ance vs Wavelength or Time may be converted to first or second derivative using the Replot feature, also described in Paragraph 2.4. An automatic Peak Pick computation is performed for each scan to determine the wavelength at which up to ten of the most significant peaks appear. The wavelenath and reading for each of the peaks are displayed on the hard copy printout of each soan and in the Replot mode. Feeplot mode is also used to facilitate scale expansion. ‘The wavelength ang absorbance ranges may be changed to accommodate the data and optimize the display as described in Paragraph 2.4.2. 1.5 INSTALLATION INFORMATION Select a location for your instrument which has a fairly constant temperature, and is away from heating and cooling vents or windows. Also, the location should be reasonably free of dust, excessive moisture, and cor- rosive chemicals, Keep in mind that instrument pertorm- ance may be affected by strong electromagnetic fields that may exist in the proximity of large electric motors, diathermy machines, or microwave sources.OPERATION ‘The DUB-6 Spectrophotometer features a CAT display which indicates analysis parameters, and operational prompts before and during the analysis, and displays the collected data upon completion of a test. The DU-6 ‘Spectrophotometer keyboard is used to select or edit analysis parameters in the creation of an analysis pro- gram. After selection of an analysis mode, a list of parameters is displayed on the CRT screen. The param- ‘ter to be modified is indicated by an inverse video field, fr cursor. Entries are made with either the NUMERIC. keys, or the [SEL] key on the keyboard. (The [SEL] key is used to call up the available options for bracketed parameters.) After each keystroke, the cursor will indi- tale a new option until all the options have been shown, After a complete cycle of options has been displayed, the cycle repeats. Each entry must be followed by depression of the ENTER key. After a parameter is modi- fied or edited, the cursor automatically moves to the next parameter. The cursor may be repositioned manually using the four ARROW keys. Each depression of an ARROW Koy. moves the cursor one available position in the direction of the ARROW. Use of the cursor, with the [SEL], NUMERIC, ARROW, and ENTER keysis the same in each of the analysis, data presentation, and memory modes. ‘The following paragraphs describe the use of each analysis mode as well as the subsequent presentation of the data, Data presentation functions which are available in the Wavelength (x) Scan, and Time Drive modes with the addition of the accessory Printer/ Plotter and Quant | Accessory are discussed in Paragraph 2.4, Use of tho optional instrument memory (Quant | Accessory) for storage and recall of specific programs is discussed in Paragraph 26, and use of the optional Scan Search feature Is described in Paragraph 25. NOTE Some of the accessories available for the DU-6 Spectrophotometer include the Quant | Accessory features. For instance, Quant il and Il Analysis Accessories and some Gel Scan and Kinetics Analysis Accessories allow access to all of the Quant | storage and calculation functions. If you havo one of these acces- sorles instead of the Quant I accessory, you should read carefully the sections in this manual which refer to use of the Quant I ‘Accessory features. 2.1 INITIAL START-UP PROCEDURE After connection of the power cord to an appropriate ‘source, the instrument will attain Idle mode. Once power is applied, it should remain on (in Idle) except for ‘extended periods of inactivity, or when the instrument is, serviced. This will provide accurate and consistent performance, and will avoid unnecessary wear on the system. Always allow for thirty minutes of warmup time after the instrument has been off or in Idle mode for an ‘extended period. SECTION TWO ‘The DU-6 Spectropnotometer performs a series of diagnostic selt-checks when system power is turned on. (Diagnostic checks are described in Section Three of this manual.) If any instrument malfunctions are detected, an error message is displayed on the CRT. If this occurs, refer to the suggested procedures in Section Four of this manual. To begin an analysis: Make sure that the instrument is plugged into a power source of the voltage specified for the unit. o— Depress the ON/IDLE key on the instrument keyboard. The instrument homes all motors, and displays a list of operating instructions. OND (Le ‘Turn on appropriate sources using the kays on the upper left-hand section of the keyboard. (The UV key is not ‘operational on the DU-6 Visible Spectrophotometer.) An Indicator at the bottom of the CRT screon will flash until the UV lamp has lit, When the symbols "UV" and/or "VIS" remain constant, the respective lamp is lit w vis The user now has the option of selecting one of the four available operational modes, or obtaining a list of the existing programs from the instrument memory. Paragraph 2.2 provides detailed instructions for creating ‘@ new program and collecting data in each of the oper- ating modes. list of existing programs may be obtained by depressing the STORE key, when the Quant | Acces sory is installed. ar i2.2 ANALYSIS PROGRAMS AND DATA COLLECTION Operating mode is chosen by depressing the appropriate key on the lett-hand section of the keyboard: Single A, Scan, or Time Drive. At this point, the analysis parameters will appear and may be edited to fit the specitic analysis requirements. To use an existing program, depress the operating mode key, then enter the storage number of the program. All parameters will automatically reset to the ostablished values. Numerical entries (symbolized by (2) in the following) are made with the NUMERIC keys on the keyboard, and ‘must be followed by the ENTER key. Bracketed parameters aro called-up using the [SEL] key. When the appro} value appears, depress tho ENTER key. ARROW keys (symbolized by [{] ) may be used to reposition the cursor on the CRT, 2.21 SINGLE WAVELENGTH (1) MODE ‘The Single Wavelength mode is used to obtain a % transmission, absorbance, or concentration reading at one anal cal wavelength. Use the following sequence to create a Single Wavelength analysis program, SINGLE] Depress the SINGLE 2 key on the instrument keyboard The CRT displays available parameters. © Enter a storage identification number: + ENTER ® Select the desired mode for data collection, Abs or %T: 608 © Ise) ENTER] © Enter the analytical wavelength (190 to 800 nm or £325 to 900 nm): * ENTER. @ In Abs mode, tho concentration calculation may be ither FACTOR or STANDARD (Paragraph 1.4.1). (Use NONE if no calculation is to be performed): (seu) ENTER] Then enter the desired value for the calculation option chosen: enten| Make sure that the samplo compartment is empty or contains only the blank or reference solution. Close lid Depress the START key to calibrate the Instrument, [sr]When the CRT prompt appears in the upper right-hand corner, insert sample (or standard) into the sample compartment, then close lid securely. Depress RUN key to obtain the reading. Place each additional sample into the sample compartment, close lid, and depress RUN key to obtain the reading. The results of each sample measurement are immedi- alely displayed on the CRT screen, In this example, a “standard” calculation has been used to determine the concentration, resulting in a factor of 10.6. 2.2.2. MULTI WAVELENGTH (1) MODE ‘The Multi Wavelength modo is used to take readings at as many as eight (8) distinct analytical wavelengths. Use the following sequence to create a Multi Wavelength analysis program. enn Depress the MULTI a key on the instrument keyboard. ‘Tho CRT displays available parameters. © Entor a storage (identification) number: 2 ENTER, secvmnm ous areconornoraneren | ry @ Selent the desired mode for data collection, Abs or 267: L@ wai] [aves] . ENTER © Enter the values for the analytical wavelengths (these may be entered in any order): + ENTER,Make sure that the sample compartment is empty, or Depress the RUN key to obtain the reading. Place each Contains only the blank solution. Close lid. additional sample into the sample compariment, close lid, and depress RUN key to obtain the readings. Depress the START key to calibrate the instrument. Fun staat] ‘The results of each sample measurement will be imme- aibaty aiepved on Wi GHT potoen a win asap When the CAT promot appear, ingot the sample into Sal apived ‘a he sampt compartoant ten love the 2.2.3 SCAN MODE ‘The Scan mode Is used to obtain readings of absorbance, % transmission, first derivative or second derivative of absorbance vs wavelength for any wavelength interval. (With the Quant | Storage Accessory, scan data Is stored in the instrument memory automatically until a new scan is run.) Use the following sequence to create a Scan program. Depress the SCAN key on the insuument keyboard, ‘The CAT displays available parameters. © Enter a storage jentification) number: * ENTER, © Select the ai 1 Der, or2 Der: for data collection, Abs, %T, tseyy | [exter © Enter the Starting and Ending analytical wavelengths. ‘The Starting wavalength must always be at least 10 nm ‘greater than the Ending wavelength: O88 d ENTER, @ Select the scanning speed, 200, 150, 60, 30, of 18 nm per minute: seq) || enren © Enter the upper and lower limits for the y-axis (~3.0 to .0A oF Oto 200 %T):‘Make sure that the sample compartment is empty and. that the lid is closed. Then depress START key to cali= brato the instrument. stant] ‘When the prompt appears, place the blank or reference solution in the sample compartment. (Air reference may also be used.) Depress the RUN key to obtain the background scan. [me] Place the sample in the sample compartment. [~] Depress the RUN key to obiain the scan ofthe sample. (The accessory Printer/Plotier must be used in con Junetion with the DUS Spectrephotometer to obtain @ graphic plot ofthe data) With the optional Quant | storage and Calculation Accessory installed, Peak Pick results may be displayed at the completion of the sample scan by depressing the REPLOT key. The most significant peaks (up to ten) are Identified automatically for the original scan range. PERE PICK a aes as Abie 1.198 455.0. 1.075 447.5 2.485 ‘The scan range may then be changed while the instru- ment is in Replot made, by entering new wavelength values with the NUMERIC and ENTER keys. Poak Pick values will be updated to include the ten most significant peaks in the new range. ‘The Scan Search feature, Paragraph 2.5, may be usod at this point to incromont through the data and obtain specific readings, To convert Absorbance data to First or Second Deriv- ative data while in Replot mode, use the [SEL] and ENTER keys to change the function option as needed.2.2.4 TIME DRIVE MODE The Time Drive mode is used to monitor absorbance, % transmission, or first or second derivative of absorbance a a function of time at a speci 1d wavelength. Readings may be taken at a rate of 1, 10, 60, 120, or 300 per minute for a total analysis time of up to 1200 minutes. Use the following sequence to create a Time Drive program, 10 ddddod Depress the TIME DRIVE key on the instrument keyboard. ‘The CRT displays availablo parameters. © Entera storage (identification) number: = ENTER © Select the desired mode for data collection, Abs, %T, 1 Der, or 2 Der: tse) || entea © Enter the analytical wavelength: * ENTER, @ Select the Rate of data collection, 1, 10, 60, 120, or 300 per minute. tet) | | enten © Enter the total time for the analysis, up to 1200 minutos: . ENTER, © Enter the upper and lower Limits for the y-axis: * ENTER Make sure that the sample compartment is empty or contains only the blank solution. Close li. Depress START key to initiate instrument cal sranr] When the prompt appears, insort sample into the sample compartment. Close lid securely. Depress the RUN key to begin the sample readings. RUNWith the Printer/Plotter accessory graphically displ stalled, results are /od as the analysis proceeds. When a Kinetics Analysis accessory is installed, the i Tabulation mode may also be used to display data. In the Tabulation mode, the data is displayed page-by-page. To change to the next “page” in the sequence, position the cursor over Page" on the display, and depress the “up” or “down” arrow key to access the adjoining page in the sequence, (Page One) (Page Two) 42.3 AUTO ZERO FEATURE ‘The Auto Zero feature is a convenient way to compen- sate for instrumental baseline drift. In any mode, the DU-6 Spectrophotometer may be reset 100.0 or 100.%T by depressing the AUTO ZERO key. The instrument is then ready to resume collection of sample data. ‘The Auto Zero feature may also be used to reset to 0.0 A (or 100 %7) after a chango of blank or reference solution. This may be done only if the new and old blank solutions have similar spectral characteristics. Using the ‘Auto Zero feature to compensate for large differences in blank solutions may affect instrument accuracy. ‘Auto Zero is much faster than recalibration and there- fore can greatly reduce analysis time. However, when greatest accuracy is required, a complete recalibration, Using the START key, should be performed, 2.4 DATA PRESENTATION WITH OPTIONAL PRINTER/PLOTTER When the Printer/Plotter accessory (Beckman Part 96148 or 596144) is installed and used in conjunction with the DU-6 Spectrophotometer, additional data pres- entation options become available. These are discussed in the following paragraphs. In general, any information which is displayed on the DU-6 Spectrophotometer CRT may be transferred to and recorded by the Printer/Plotter. To do so, depress the COPY key on the keyboard. 2.4.1 AUTOMATIC SCAN PLOTTING When the Printer/Plotter is powered-up and on tine with the DU-6 Spectrophotometer, all data taken in Scan or Time Drive operational mode will be automatically plotted. The Printer/ Plotter produces a real-time graphic plotof absorbance, % transmission or derivative readings vs wavelength while the 4 Scan data is being collected by the instrument. An example is shown in Figure 2-1. In Figure 24. Plotot Sean Data 12 addition to the plot of the data, the optional Peak Pick values calculated with the Quant | Accessory are tabu- lated for each scan at the bottom of the printout. In the Time Drive operational mode, a plot of the absorbance, % transmission, or derivative data vs time |s created, as shown in Figure 2-2, Fiouro 22. Plotot Time Brive Date Bocause these graphic printouts are created automa- tically while the data is being collected, no operational commands are necessary. 2.4.2 REPLOT FEATURE (OPTIONAL) ‘The Replot feature Is available when both the Quant 1 Accessory Package (Beckman Part 596148) and the Printer/Plotter are used In conjunction with the OU-6 ‘Spectrophotometer. ‘Once a plot from a scan has been obtained using the DU-6 Spectrophotometer and the Printor/ Plotter, depress the REPLOT Key on the instrument keyboard. This allows the analyst to reset tho paramotors of the analysis (function, wavelength range, and upper and ower limits) and obtain a modified or expanded plot of the data, When the wavelength range is modified, the Peak Pick values (if applicable) are updated to include the ten most significant peaks within the new range. The Scan Search feature may also be used to obtain specific data points. ‘To ecit the parameters for an expanded or converted scan, use the same procedure as you would in creating 8 new program. The cursor field indicates the parameter to be edited, and may be repositioned with the ARROW keys. New values are entered, as necessary, using the NUMERIC, [SEL], and ENTER keys. After the values aro establishod, depress the RUN key to obtain a new graphics plot.WN 2.5 SCAN SEARCH FEATURE (OPTIONAL) ‘The Scan Search feature, also available with the Quant | Accessory, may be used after data has been collected In either the Scan or Timo Drive operating modes. Scan ‘Search controls are located in the upper center section of the keyboard, and the values are displayed in the lower, right-hand section of the CRT. Scan Search allows the operator to manually incre- ment through the readings for each wavelength or time in the original scanning range. The wavelength (or time) display is controlled by depressing one of the Scan ‘Search ARROW keys. To increment up to a higher wave- length or time, depress the right-hand ARROW key. To dacrease the wavelength or time, depress the left-hand ARROW key until the desired value appears. 2.6 STORAGE AND RECALL OF DATA AND PROGRAMS Up to 89 frequently used analysis programs may be stored in the OU-6 Spectrophotometer memory when the Quant | Accessory is installed. (Refer also to Beckman Instructions 015-245977, Quant | Storage Accessory instructions.) The program Is then recalled by storage Identification number for use. Each Wavelength Scan or Time Drive Scan is auto- matically stored, and is held in the instrument memory until the next scan is run 1 STORING A PROGRAM Use the following sequence te store an analysis program, 2 RECALLING A STORED PROGRAM After the desired program parameters are sat, depress the STORE key on the instrument keyboard. STORE Using the NUMERIC and ENTER keys, enter the storage Identification number (between 1 and 99) for the program at the bottom of the Storage Display Screen. NOTE A unique number must be chosen for each program! Stored programs are recalled from memory using the following procedure. SCAN > #01 FUNCTION (abs? STARTING 475) ENDING 425 SPEED sol UPPER LIMIT 3.000 LOWER LIMIT 8.000 Depress the key for the operating mode (Single a, Multi, et.) of the stored program. When the program parameters appear, enter the storage: identification number for the stored program. Tho parameters automatically reset to those of the stored program. [pre 132.8.3 DELETING A PROGRAM Stored programs may also be deleted from memory by the following sequence. 2.6.4 DELETING A SCAN Depress the STORE key on the instrument keyboard. Use TORE the [SEL] and ENTER keys to modify the option field at the bottom of the display to [Delete Program), Use the NUMERIC and ENTER keys to enter the storage Identification number of the program. Scan data is automatically stored in the instrument memory, therefore no manual storage commands are required, However, scans may be manually daleted as follows. 14 Depress the STORE key on the instrument Keyboard. STORE Use the [SEL] and ENTER keys to modify the option field at the bottom of the display to [Delete Scan], The scan is now deleted from memory.AUTOMATIC INSTRUMENT FUNCTIONS During normal operation, the DU®-6 Spectrophatometer Performs @ number of automatic self-calibration rou- tines. This section is provided to give the user a belter understanding of the instrument. Experience has shown that an operator who understands how an instrument works is able to get consistently better results and is. better propared to recognize maladjustments or misuse cf the instrument, 3.1. START-UP CHECKS Each time the DU®-6 Spectrophotometer is turned on, it automatically performs a series of checks to verity proper operation. The items monitored include: RAM (Random Access Memory) ROM (Read Only Memory) . Internal timer . Video display system . Peripheral processor . Filter position Wavelength position Signal-processing electronics . Optics Source Mirror 11. Sources If any malfunctions are found, an error message will be displayed on the CRT screen. Refer to Section Four tor malfunction explanations, 93.2 CALIBRATION ROUTINE Atter the desired parameters aro established, the START key is depressed to calibrate the instrument. The instru- ‘ment automatically performs the following steps: 1. Dark current is measured and corrected. This, compensation assures accurate readings at high absorbance, 2. The monochromator is moved to the proper wave- length. 3. The proper detector attenuation value is selected, ‘This minimizes the noise level and maximizes pho- tometric accuracy, 4, Tho log converter (absorbance-based modes, only) is calibrated to provide linearity throughout the operating range. ‘These steps assure proper instrument performance everytime the instrument is used; day-to-day and year~ to-year. SECTION THREE 3.8 BACKGROUND CORRECTION (Scan Mode Only) [At the completion of the calibration, Insert Background- Push Fun” appears in the upper right hand corner of the CRT screen, At this time, the blank or reference solution should bo placed in the sample compartment. When RUN Is pushed, a complete scan of the blank ia initiated, and siored in tho instrument memory. All subsequent sample measurements are corrected for background against this scan 3.4 AUTOMATIC BLANKING (AUTO ZERO) In any mode, the instrument can be reset to OA or 100 %T by placing the blank solution in the sample compartment ‘and pushing the AUTO ZERO key. In less than two sec- ‘nds the DU-6 Spectrophotometer is ready to resume the collection of sample data, Refer also to Paragraph 2.3. 15DIAGNOSTIC SECTION FOUR AND ERROR MESSAGES 4.1 POWER-UP DIAGNOSTIC MESSAGES Error messages which appear during the initial power- Up of the instrument indicate failure of a circuit board. ‘The messages are described below to help the user locate the problem. Replace the circuit boards as indl- cated, using the installation instructions provided with ‘Some error messages indicate a possible fallure at more than one location. If this is the case, as indicated under Possible Location of Failure in Figure 4-1, replace the components in the samo order as they are listed until the instrument performs properly. Diagnostic tests are Performed sequentially until a failure is detected, and an error message displayed, Thus, if more than one cam- Ponent fails, only the first error will be displayed until the problem is corrected, at which tima a secand error ‘message will appear during power-up. the board, or call your local Beckman Service Office. Nore I the beam path is blocked or sample compart- ‘ment tid is open, failure messages will ovour Nore tthe Kinetics il Analysis Accessory is installed, erroneously. always power-up the main instrument belore the Kinetics Control Unit. Otherwise error mes- ‘sages may occur erroneously. ERROR POSSIBLE LOCATION MESSAGE SUMMARY OF TEST ‘OF FAILURE ERROR 1 (CPU Board: RAM and ROM are tested nondestructively; Interrupt 1, CPU Board Controller is tested; Interval Timer is tested. (Bockman Part §94042) ERROR 2 CRT Board: RAM (character and graphics) is tested nondestructively; 1. CRT Board CRT controller is tested (Beckman Part 584136) ERRORS 1/0 CPU Tost: The CPU on the I/© Board Is tested 1. 1/0 and Analog Boara (Beckman Part 896112) ERROR 4 Detector Compartment: ADC is tested to read output from the photo- +, 1/0 and Analog Board diode with detector compartment dark (Beckman Part 696112) 2, Preamp Board (Beckman Part 696114) 3, Light leakage. ERRORS — Preamp/Preamp LED: ADC readings with preamp LED on andolf are 4. Preamp Board compared for significant difference. (Beckman Part 596114) 2. 1/0 and Analog Board (Beckman Part 596112) ERROR 6 Wavelength and Filter: Wavelength and Filter drives are tested for 1. 1/0 and Analog Board funetionality (Beckman Part 596112) ERROR7 —_ Filter Home: Filter position is tested. 1. 1/0 and Analog Board (Beckman Part 596112) ERROR 8 Visible Source: Readings are taken with the beam blockad and un- 1. Sample or othar blocked by the filter, and compares for significant increase. (NOTE: ‘obstruction is in the Ifa sample is blocking the beam, this message may appear errone- sample compartment. ously — remove any obstruction if the prompt appears.) 2. Visible (Tungsten) Source (Beckman Part 873947) 3. 1/0 and Analog Board (Beckman Part 596112) 4. Source mirror alignment. Figure 41. Disgnottic Error Messoges continued, next page. W7ERROR MESSAGE SUMMARY OF TEST POSSIBLE LOCATION OF FAILURE ERROR 9 ——_UV Source Mirror: Readings from visible source (on) and trom UV source (off) compared for significant decrease. ERROR 10 Visible Source Mirror: Readings from UV source (off) and from visible source (on) are compared for significant increase. 1/0 and Analog Board (Beckman Part $8612) Source mirror alignment. 1/0 and Analog Board (Beckman Part 686112) Source mirror alignment. Figure 4. Diagnostic Error Messages (Continued) ‘An obvious problem during power-up (@.g., no instru- the fuses (Including the main Power Fuse) and replace It ‘ment response, blank GRT, etc, indicates either atailure necessary. If this does not correct the problem, replace of one or more of the fuses, or within the Power Supply the Power Supply Board. Board (Beckman Part 596026). When such a failure is, Indicated, first, check that the power to the instrument is adequate. Then disconnect instrument power, inspect“4.2 MESSAGES DURING OPERATION The following messages occur while operating the DU-6 Spectrophotometar. Unioss otherwise noted, the instru- ment takes no further action and walts for additional Input betore execution of the last command. MESSAGE PROBABLE CAUSE/DISCUSSION ‘OPERATOR ACTION Outof-Range Incorrect Format, Source Not On Vis Source Failure UV Source Failure Press START Insufficient Memory — File Exists ‘Warning — Range Limit XXA = KOK Warning — Low Energy Attenuator Overrange (0%T Offset Failure Value entered which is outside specified limits. Field is blanked. Numerical value entered with incorrect number of digits to either side of decimal point. Field is blanked. START Is pressed before source is turned on. Source fails to fire immediately, Source fails to fire within § minutes. AUN is pressed after program parametor ‘modification. Run is not executed, Insufficient storage space to ‘accommodate Program or Scan. File name (number) has already boon assigned, Upon second attempt, old file will be deleted and new file will be stored under same assignment. Background has measured more than 1,04. Instrument may not meet specifications for absorbances greater than X.XA as displayed. Instrument was unable to calibrate with @ large enough signal to meet ‘dynamic range. Excessive light energy, pre-amp zero misadjusted, Analog Board failure. Light leakage, or Analog Board failure, Enter new value within specified parameter limits. Enter new value in correct format for parameter. Turn on source, wait until it has fired to press START. Check VIS source, replace if necessary. Check UV source, replace if necessary. Press START. Allow to re-calibrate. Press RUN. Delete files to free-up memory, or reduce memory requirements of scan, {Increase Scan Speed or Decrease Wavelength Range) User may do any of the following: — Assign a new storage number. — Manually delete old file. — Automatically delete old file and replace by a second attempt. User may do any of the following: = Dilute background sample. — Scan a shorter range. User may do any of the following: = Proceed with caution. — Dilute background sample. — Remove any obstruction, if applicable. Roplace Analog I/O Board (Beckman Part 596112). Chock for Light Leaks. Fieplace Analog 1/0 Board (Beckman Part 596112) Figure 42, Operational Error Messages 19MAINTENANCE PROCEDURES This section includes detailed repl you! this structions for routine intenance procedures such as fuse and source lacements. Replacement paris may be obtained from ir Beckman Representative. (Refer to Section Six of ‘manual for part numbers.) ‘CAUTION ALWAYS DISCONNECT INSTRUMENT POWER. BEFORE PERFORMING ANY OPERATION WHICH REQUIRES THE REMOVAL OF THE INSTRUMENT CASING. Imp jortant Note: When instrument power is disconnected and/or instrument cover is removed, all information stored in memory lost unloss the Memory Protect ‘Accessory (Beckman Part 594098) is engaged. 5A SOURCE REMOVAL AND REPLACEMENT WARNING. BEFORE ATTEMPTING TO REMOVE OR HANDLE THE SOURCE, ALLOW INSTRUMENT TO COOL WITH POWER OFF FOR AT LEAST FIFTEEN (15) MINUTES. Stop 1: DISCONNECT INSTRUMENT POWER. ‘Step 2: Remove the four (4) cover screws located on the outside of the instrument (see Figure 5.6, page 23). Allow the instrument to cool for at Teast fifteen (15) minutes. Make sure that all sample compartment accessories have boon disconnected, Then lift he entire cover straight up to remove. This will expose the source ‘compartment cover located on the center, front of the instrument, Step 3: REMOVE SOURCE COMPARTMENT COVER Using a Phillips-head screwdriver, remove the four (4) screws on the source compariment cover, Figure 5-1 ove Serews ee oe 7 5 | ° = ee Four 195-1, Source Compariment SECTION FIVE Pull the source compartment straight out, being careful not to strike either bulb against the surrounding compartment. DO NOT touch the source mirror with any object during this procedure. Continue with Paragraph 5.1.1 (Visible Source) or 5.1.2 (UV Source), following 5.1.1 VISIBLE (TUNGSTEN) LAMP REPLACEMENT Stop 4: LOCATE THE TUNGSTEN SOURCE Refer to Figure 5-2. Note the orientation of the bulb and manufacturing label Fioure 52, Visible Source Step 5: REMOVE OLD BULB. ‘After the bulb has cooled, grasp firmly and pull ‘out of the holder. ‘CAUTION DO NOT TOUCH NEW BULB WITH FINGERS! Use either the packing sleeve or a cloth to handle, if bulb is accidentally touched, clean ‘caratuily and thoroughly with alcohol and a lint-free cloth. Step 6: INSTALL NEW BULB Push new bulb firmly into position until it is completely against the stop and oriented as was the old bulb. Step 7: VERIFY ALIGNMENT Ensure that the manufacturer's label tacos away from the mirror. Continue with Paragraph 5.1.3, Cover Replacement a5.1.2. UV (DEUTERIUM) SOURCE REPLACEMENT (This procedure to be performed only on DU-6 UV-Vis Spectrophotometers.) Perform Steps 1, 2, and $ as directed above, referring to Figure5-1 as needed Step 4: LOCATE AND REMOVE OLD SOURCE Refer to Figure 5-3. Ceretully note the orienta- tion of the old bulb, especially the internal components. Disconnect the four-wire plug from the compartment cover, and loosen the two (2) clamp screws. Carefully slide the old source out of the clamps. connection Figures. uv source CAUTION DO NOT TOUGH THE NEW BULB! I this should accidentally happen, clean carefully with alcohol and a lint-free cloth. Step POSITION NEW SOURCE Handling $0 as to avoid touching the bulb, slide the new bulb into position between the ‘two clamps. DO NOT tighten the clamp screws. ALIGN THE SOURCE Slide the new Bulb up or down until the center hole in the light source is on the center line indicated in Figure 5-4. The upper row of adjusting screws that position the source holders are a convenient reference for estab- lishing vertical alignment. Without moving the lamp vertically, rotate the bull until the oval face of the light source is. pointing directly at the mirror. Recheck vertical alignment with center line and adjust, if necessary, Stop 6: Step SECURE BULB IN PLACE Tighten the clamp screws so that the bulb is held securely but not too tightly — bulb may break if clamp is too tight! Reconnect the power leads to the outlet in the compartment cover. Continue with Paragraph 5.1.3. CD settce ‘Tungsten loge Lamp Deuterium (uv) Lam (conte itror Adjost Figure s4, UV Source Algnment 225.1.3 SOURCE COMPARTMENT COVER REPLACEMENT Step 8: POSITION SOURCE COMPARTMENT COVER Using the solenoid link (outside top of com- partment) position the source arm slat to open towards the center of ihe compartment, Figure 5: Step 9: Step 10: Stop 11: Figure 6S. Gover Replacement While holding the arm in this position, replace the compartment cover making sure'that the mirror pivot slides into the slot in the arm. Verify that the pivot and arm are properly in place by pulling the solenoid link to the left and releasing — it should return immediately to the original position SECURE COVER Replace the four (4) source compartment cover screws, and tighten securely. REPLACE INSTAUMENT COVER Align the cover screw holes on the instrument cover with the screw holes on the base Replace the cover screws ana tighten securely. Replace sample compartment accessories If necessary, PERFORM PEAKING PROCEDURE, PARAGRAPH 5.2 ‘The Peaking Procedure must ALWAYS be per- formed after source replacement to ensure ‘optimum energy from the lamp. over Serows ‘Aooeed Panel Sree Figure 5:6, Source Aecoss Pane! 235.2 PEAKING PROCEDURE This procedure is to be performed with the instrument cover in place and secured by the four (4) cover screws. The Peaking Procedure must be performed after a lamp has been replaced, and may also be per- formed periodically to ensure maximum radiant energy from the source or sources. Refer to Figures 5-6 and 5-7 ‘as necessary. serew 8 (isan) Screws Screw A Screw D (WV tamp) (is Lame) Lemp) Figura 521 Step 1: Step 2: Step 3: 24 7. Peaking Screws PEAKING THE VISIBLE (TUNGSTEN) SOURCE APPLY POWER, Reconnect instrument power and turn on the Visible Source. Allow at least thirty (80) minutes for the source to warm up. REMOVE ACCESS PANEL Using a Phillips-head screwdriver, remove the two (2) screws on the access panel, Figure 5-6, then pull off the panel (NOT the’ instrument cover!) ‘SET PARAMETERS Set program parameters using the same proce- dure as for a normal “Single \” analysis. Use 7” for the FUNCTION option and a WAVE- LENGTH of 486 nm. Refer also to the Proce- dure Summary. ADJUST PEAKING SCREWS Using the %-inch nut driver wrench provided In the shipping kit and referring to Figure 5-7, adjust the screws as follows: Turn Screw C to febtain maximum %T reading on CRT. Then adjust Screw A, then Screw B, to maximize the value. Return to Screw A and maximize the %6T reading, then repeat the adjustment procedure until no further increase in energy is oblained. ‘PROCEDURE SUMMARY MODE: Single FUNCTION: 488m WAVELENGTH SEQUENCE: CABACAA Stop 5. NOTE: Restart the pragram (depress START) to reset the 100 %T value i the displayed read- Ing exceeds 200 %T during adjustment. REPLACE ACCESS PANEL Hold the panel in place and secure using the two screws. 5.2.2 PEAKING THE UV (DEUTERIUM) SOURCE Step 1: Step 2: Step 3: Step 4: APPLY POWER, Reconnect instrument power and turn on the UV source. Allow at least thirty (30) minutes for the source to warm up. REMOVE ACCESS PANEL Using a Phillips-head screwdriver, remove the two (2) seraws on the access panei, Figure 5-6, then pull off the panel (NOT the instrument cover!) SET PARAMETERS Set program parameters using the same proce- dure as for a normal ‘Single 4” analysis. Use '%T" for the FUNCTION option, and a WAVE- LENGTH of 800 nm. Refer also'to the Proce- dure Summary. ADJUST PEAKING SCREWS Using the %-inch nut driver wrench provided In the shipping kit and referring to Figure 5-7, adjust the screws as follows: Turn Screw F to obtain @ maximum %T reading on the CRT. Then turn Screw D, followed by Screw E to maximize again. Return to Screw D and adjust to the maximum value, then repeat the adjust- ment sequence until no further increase in energy is obtained, PROCEDURE SUMMARY MODE: Sinste» FUNCTION: set WAVELENGTH "300 am SEQUENCE: FED FO-ED Step 5: NOTE: Restart the program (depress START) to reset the %T to 100 if the displayed reading exceeds 200 %T during adjustment. REPLACE ACCESS PANEL Hold the panel in place and secure using the two screws.5.9 MAIN POWER FUSE REMOVAL AND REPLACEMENT ‘An obvious malfunction of the Instrument during power- up (e.g., no Instrument response, blank CAT, etc.) may indicate failure of the main power fuse. If this occurs, verify that the instrument Is receiving adequate power, then replace the main fuse using the following procedure. Stop 1: DISCONNECT INSTRUMENT POWER, Step 2: REMOVE INSTRUMENT COVER Disconnect any sample compartment acces- sories and cables. Orient instrument so that back panel is readily accessible. Remove the four cover screws located on the autside cover. Lift entire cover straight up to remove. LOGATE MAIN POWER FUSE Main cower fuse is located in the middle at the ‘extreme rear of the instrument, Refer to Figure 5-8 REMOVE FUSE HOLDER Insert a flathead screwdriver into the slot at the top of fuse halder, and turn (counterclockwise) Until top is released (about one-quarter turn, Step 5: REMOVE FUSE FROM HOLDER, REPLACE See Section Six of this manual for Beckman Part number. Note orientation of fuse, then replace new fuse into same position. Always use a fuse of same type and rating, Stop 6: REPLACE FUSE HOLDER Place holder back into sleeve, then secure in place using a screwdriver. (Clockwise, about one-quarter tum.) Step 3: step. Step 7: REPLACE INSTRUMENT COVER Bo sure that cover scrow holes line up on base and cover. Replace four cover screws, and reconnect any sample compartment acces- sories and cables, if applicable. ‘Step 8: RECONNECT INSTRUMENT POWER If this procedure has not remedied the problem, contact yaur local Beckman Represantative, or replace Fuse Bank fuses as directed in Para- graph 5.4 5.4 REPLACEMENT OF FUSE BANK FUSES Roplace fuses on the Power Supply Board Fuse Bank when indicated by an instrument malfunction during power-up (which was not remedied by replacement of the main fuse) or when directed to do so by a Beckman Service Representative. Replacement fuses are provided in the Fuse Kit, Bockman Part 08031, and may be ‘ordered through your Beckman Representative. Step 1: DISCONNECT INSTRUMENT POWER ‘Step 2: REMOVE INSTRUMENT COVER AND ALLOW TO COOL Remove the four (4) cover screws located on the outside cover, and be sure that sample compartment accessory cables have been dis- connected. Lift the instrument cover straight up to remove, and allow at least fifteen (15) minutes for the components to cool. Orient the instrument so that the back is readily accessible, Step 3: LOCATE THE FUSE BANK Eight (8) of the fuses are located along the Upper left-hand section of the Power Supply Board (directly behind the CAT, Figure 5-3), and one additional fuse is located below these. Fuse Bank ain Fuse Figure 58. Main Power Fuse Figuie $9. Location of Fuse Bank 25Step 4: INSPECT FUSES Inspect the individual fuses for a source of malfunction, such as improper positioning in holder, broken filament, etc. The individual fuses may be removed and checked for func- tionality with an ohmmeter, it available; how- ever, this is not necessary, Step 5: REPLACE FUSE OR FUSES Ian obvious defect was found during inspec- tion, then only the defective fuse need be replaced. If, however, the malfunction cannot be traced to @ single fuse, replace all nine (9) of the fuses as follows, REMOVE OLD FUSE Remove the fuse from the holder by pulling it straight out from the holder. Note the identifi ‘ation numbers on the ends of the fuse as shown in Figure $-10, step. Figure £70. Fuse identiiestion INSTALL THE NEW FUSE Locate the fuse in the fuse kit with the same identification number (i.e. same type and rat- ing) as the old fuse. The rating of the fuse is also printed on the Power Supply Board imma- diately above the fuse holder. The new fuse should snap completely into place and resist further movement. REPLACE INSTRUMENT COVER Be sure that the cover seats firmly on top of the instrument, and that cover screw holes line up on the base and cover. Replace the four (4) cover screws. Remember to install accessory ‘ables and tubing in the sample compartment, applicable. RECONNECT INSTRUMENT POWER Allow instrument sufficient time to warm up, then check instrument performance. If this pro- ‘cedure has rot remedied the problem, contact, your local Beckman Service Representative, Step Step step 26 5.5 REPLACEMENT OF 220-VOLT FUSE (220-V INSTRUMENTS, ONLY) This procedure is to be performed ONLY on those instru- ‘ments which are designed for use with @ 220-volt power supply source. Before attempting to perform this proce- dure, verify the instrument's specific voltage require- ments (embossed on the name rating plate on the back of the instrument), Figure 6-11, Step 1: DISCONNECT INSTRUMENT POWER ‘Step 2: REMOVE INSTRUMENT COVER AND ALLOW TOCOOL Remove the four (4) cover sorews located on the outside cover, and be sure that sample ‘compartment accessory cables have been dis- connected. Lift the instrument caver straight up to remove, and allow at least fifteen (15) minutes for the components to cool, Oriont the instrument so that the back is readily accessible, Figure 5-11. Pawar Supply yf Name Rating Piste. Figure 5:1. Rear Exposure, Power Supply Board DISCONNECT POWER SUPPLY BOARD Disconnect the Power Supply Cable by grasp- ing firmly, and pulling straight up. Loosen the thumbscrew to free the Power Supply Board, Figure 5-12, REMOVE POWER SUPPLY BOARD. Remove the Power Supply Board completely from the instrument, Figure 6-13, Be careful not to catch any of the Components on the support clip which secures the board at the bottom. LOCATE THE 220-VOLT CONVERSION FUSE HOLDER ‘The 220-volt conversion fuse holder is directly in front (towards the CRT) of the main powor fuse, Figure 5-13. It Is only visible with the Power Supply Board removed Step 3: Step 4: Stop 8:Cable, \ \ paca Figure 5-12. Preparation for Removal ‘Step 6: REMOVE THE FUSE HOLDER Insert a standard screwdriver into the slot at the top of the fuse holder, and turn (counter- clockwise) until top is released. REMOVE FUSE FROM HOLDER Rafer to Section Six of this manual for replace- ‘ment part number. Replace new fuse into same. position. Always use a fuse of same type and. rating. REPLACE FUSE HOLDER Place holder back into sleeve, then secure in place using a standard screwdriver. REPLACE POWER SUPPLY BOARD. Position board exactly as it was before removal. Make sure that the board seats securely on the ‘support clip at the bottom right-hand comer. ‘Secure into position with the thumbscrew. Step 7: Step 6: Step 9: Figure 619. Board Removal ‘Step 10: ATTACH POWER SUPPLY CABLE ‘CONNECTOR Re-install the cable connector at the top of the Power Supply Board, Push securely into place. ‘Step 11: REPLACE INSTRUMENT COVER Be sure that cover seats firmly on top of the instrument, and that cover screw holes line up fon the base and cover. Replace the four (4) cover screws. Remember to install accessory ‘cables and tubing in the sample compartment, it necessary. Step 12: RECONNECT INSTRUMENT POWER Allow instrument sufficient time to warm up, then check instrument performance. If this pro- cedure does not remedy the problem, contact your local Beckman Service Representative, a7i é & ne 28 a C) maSUPPLIES BECKMAN RT NO. DESCRIPTION ACCESSORIES FOR UPDATING YOUR DU-6 SPECTROPHOTOMETER: Hard Copy Options 596143 Printer/Plotter, 115-Volt 596144 Printer/Plotter, 230-Volt 897516 _Printer/Piotter Paper, 1000 fantold sheets 596149 Printer/Plotter Intertace Quantitative Analysis Accessories" 596148 Quant | Accessory 596177 Quant Il Analysis Accessory 596176 Quant Ill Analysis Accessory Sipper and Batch Sampler Accessories" ‘596145 Sipper Sampler, Rail Mount 596146 Sipper Sampler, Transport Mount 596268 Batch Sampler, 115-Volt 596269 Batch Sampler, 230-Volt ‘596264 Sipper/Batch Sample Accessory 594436 Flow Gell, Replacement 594437 _Sipper Cell Replacement Tubing (3 sets) ‘596265 Batch Sampler Replacement Tubing (3 sets) Multi-Position Cell Holders* 598141 Auto 6-Sampler 94280 Manual 6-Position Sampler 590811 Cell Basket, for use with either Sampler Kinetics Analysis Accessories* 596233 Kinetics Gel Scan Software, includes Quant | capabilities. 96228 Kinetics Il Accessory for use with Auto 6-Sampler ‘596222 Kinetics Ill Accessory 696224 Kinetics Ill Upgrade Accessory, for use on ‘Transport-equipped instruments 595987 Coll Temperature Readout Probe 590650 High Temperature Sample Holder for use wiih Kineties Gel Sean Accessories* 596262 Gel Scan Software, includes Quant requires Gel Scanning accessories 596070 Tube Gel Holder for disk gels (up to 10 om) 198649 Gel Guvotio, 7 mm pathlength 596041 Film (Autoradiograph) Holder (10 om scanning path) 596077 Gel Sits, 0.05, 0.1, oF 0.2 mm width by 05, 1, 2, or4 mm height SECTION SIX BECKMAN PART NO. DESCRIPTION Water-Regulated Cell Holders* 198157 Thermoregulated, Water-Jackeied Cell Holder for 1 em rectangular cells 596280 Water-Regulated 6-Position Gell Holder 596231 Special Sample Compartment Cover for Water-Regulated Cell Holders 196783 Universal Mount Other System Options* 594059 1.0nmSSiit 594099 Memory Protect Accessory 596140 _RS-292 Input/Output Accessory 596150 Calibration Test Accessory 596273 UV Upgrade Kit (for DU-6 Visible Spectrophotometer) “Contact your Beckman Represe ‘he applications of those arcosbor REPLACEMENT PARTS FOR THE DU-8 SPECTROPHOTOMETER ive for dataled information on 801588 Main Fuse, 115-Volt, 5 amp. 891215 Main Fuse, 230-Volt, 2.5 amp 594437 Tubing kit 86917 Pump Tubing 589784 Trace-Klean"™ 594438 Sipper Cell, Replacement 566483 Microcell, 210 mL, 2 each 586464 Microcell, 210 mL, 4 each 130767 Microcell, 280 uL2 each 130773 Microcell, 280 nL, 4 each 130768 SemicMicrocell, 350 yL, 2 each 180774 Semi-Microcell, 350 pL, 4 each 580021 Cylindrical Cell, 100'mm pathlength 580020 Cylindrical Cell, 80 mm pathlength 580019 Cylindrical Cell, 20 mm pathlength 580012 Cell, 1 cm(10 mm) 580015 Gell, 1 cm with Stopper 585699 Source Replacement, UV 879947 Source Replacement, Visible 596026 Power Supply Board Assembly 396029 CRT Board Assembly 596248 1/0 Board Assembly 598250 CPU Board Assembly 596031 Fuse Replacement Kit 290 Cy aSPECIFICATIONS SECTION SEVEN 7.1. GENERAL SPECIFICATIONS DUS.6 Visible DUS-6 UV-visible ‘Spectrophotometer Spectrophotometer Wavelength Range £325 nm to 900 nm 190 am to 800 nm Wavelength Accuracy £05 nm £05 nm Wavelength Repeatability 020m 020m Wavelenth Setabilty 0.4 nm increments 0.1 nm incroments Wavelength Resolution 2am (standard) 2am (standard) 4.0 nm (optional) 41.0 nm (optional) Photometric Accuracy (compared to =0.5% of Abs value or *0.002A, whichever is greater NBS standard SAM 90) Photometric Repeatability 0.25% of Abs value from —0.90 o 2.004 using 1 sigma method RMS Noise (600 nm, 2 nm Resolution) 0.0004 Abs (at 0 Abs) 0.0004 Abs (at 0 Abs) Stray Light -<0.1%, Blocking filter method or <0.05% ASTM, at 340mm Baseline Drift (after one-hour warmup} -<0.006 A/hr (840 nm) <<0.008 Ahr (840 nm) Baseline Flatness +0.002A from 825 nm to 750nm — =0.002A from 220 nm to 750.nm Scanning Speeds 15, 30, 60, 150, and 300 nm/min 18, 90, 60, 160, and 300 nm/min Ordinate Displays = 0.300 10 3.008 0.300 to 3.008 0.0 to 200.0 %T 0.0%0 2000 %T (00006 to 99996 (00006 to 99996 Scale Expansion 0.001 Abs increments from ~0.3 103.0 Abs ORT Size 8 inchs (22.8 em) 8 inches (22.9 cm) Optics Single Monochromator, Holographic grating with blocking filters. gle Beam. Line Power 100 oF 120V AC 12V: 50/60 Hz, § amp or 240 07 220V AC =22V; 50/60 Hz, 2.5amp Dimensions Width: 27% inches (68.9 em) Depth: 217% inches (55.6 cm) Height: 19% inches (49.8 cm) Weight: 95 pounds (43 kg) Beckman Instruments, Inc, reserves the right to change specifications without notice as part of our continuing program of product improvement. 317.2 SAMPLE COMPARTMENT DIMENSIONS. The following drawing, Figure 7- point of the beam. |, is provided to show the dimensions of the sample compartment and the focal ‘SAMPLE COMPARTMENT GEOMETRY pom mas Top view ab esp sine vew oa oP oF saupLe ;OUNTING RAS oa Figure 7-1. Sample Compariment Dimonsions ‘The overall size of the sample compartment is 5.8 inches (14 om) wide by 11.8 inches (30 em) deep by 7.1 inches (18 em) high. 32 Wr? — ewe TT oe: et 8 Ee * fe ae &. ieBeckman Instruments, Inc., Fullerton, CA 92634 July 1983 822327 015-245324 Printed in U.S.A. BECKMAN aan: Cpe Tw Can et: ler: dor: ti Mes iM Mn MAP Ri Sa: Toe; asCopsright*1973 Beckman Insirumenis, Ine Fullerton, California INSTALLATION ‘The Models 24 and 25 Spectrophotometers and. recorder ae customer installed. WARRANTY This instrument is covered by the Beckman standard war- ranty but does not include the following: 1, Replacement and installation of tungsten and deu- terium source lamps. 2. Replacement and installation of control panel indicator lamps. 3. Warranty against contamination of mirrors and other optical elements, Service of the above is available at the current labor rates. ‘A complete list of Beckman Service Offices is provided on the back page of this instruction manual.wu i Beckman ADDENDUM T0 BECKMAN INSTRUCTIONS 015-082245-A MODELS 24/25 SPECTROPHOTOMETERS ‘This addendum, alone, should not be considered to be operating instruc tions 25 Spectaphoton er, it provides eed‘MODEL 24 SPECTROPHOTOMETER, A Visible, Digital Reading And/Or Recording Spectrophotometer MODEL 25 SPECTROPHOTOMETER ‘An Ultraviolet-Visible, Digital Reading And/Or Recording Spectrophotometer INTENDED USE. ‘These devices are intended for use by operators experienced with the methodology during those parts of in vitro diag- nostic enzymatic or quantitative or qualitative analysis pro- ‘cedures which require measurement of light transmission ‘through reagents at wavelengths in the visible and ultra violet regions of the electromagnetic spectrum, ‘The Model 24 is capable of measuring light at wavelengths {rom 340 to 700 nm (190 to 700 nm with optional deuterium source). The Model 25 is capable of measuring light from 190 to 700 nen, INSTALLATION These devices may be installed by a customer, following the procedures outlined in Section Two, Installation and Check- ‘out, of Beckman Fastructions 015-082245-A, PRINCIPLES OF OPERATION ‘The Beckman Models 24/25 Spectrophotometers aredouble- ‘beam, digital reading and/or recording instruments for meas- turing light levels at specificd wavelengths. The Model 24 is ‘capable of measuring light at wavelengths from 340 10 700 ‘am (190 fo 700 nm with optional deuterium source). The ‘Model 25 js capable of measuring light at wavelengths from 190 to 700 nm. In their operation, these devices employ a tungsten light source in the visible range of the spectrum {G40 to 700 nm) and a deuterium source (standard on Model 25 and optional on Model 24) inthe ultraviolet region of the spectrum (190 10 360 nm. Light from either source is di rected to the single-rrating monochromator, which has 1200 lines per millimeter and is blazed at 250 nanometers. Con- structive imerference of a certain wavelength, other than light of the frst order, is removed via a filter wheel whi blocks second and higher-order light from reaching the sam= ple. The monochromatic light is then directed to the saraplc and reference via a vibrating mirror bridge which displaces horizontally at a certain frequeney. Therefore, this bridge allows light to pass into the sample and reference eell holders with a frequency equal to the displacement frequency of the bridge, Light transmission through the cells is detected by a side- ‘on multiplier phototube. This tube traps the photons of light Passing through the sample and reference at & photoathods, ‘The light energy absorbed by the photocathode increases the kinetic energy of electrons from the photocathode material Electrons, in proportion to the light enerey absorbed, escape the photocathode and are trapped by a dynode which has 2 high potential, The electrons and their kinetic energy cap- tured by the dynode are multiplied into a release of more ‘electrons, which pass (oa second dynode having a still higher potential than the first dynode, This dyno in tur, increases i {he mumber of eletrons passing toa suscesive dynode until the multiplication of electrons is quite larg. Finally. the electrons generated in the tube are captured at an anode which generates @ voltage pulse proportional to the total numberof electron received “The current pulses generated by the sample and reference light levels to the multiplier phototube are then amplified and analyzed in an electronic log converter. The log con- j verter transforms the light transmission current values into j ‘optical density values, where absorbance is equal to the logarithm ———raq The output from the log converter 4 is then displayed by a digital display and/or recorder. : PERFORMANCE CHARACTERISTICS ; AND SPECIFICATIONS ‘ “The quoted specifications listed on the frontis of Beckman 7 Instructions 015-082245-A apply to a properly operating device. Should device performance deteriorate to such an extent that it does not conform to the quoted specifications, with the exception of stray light, its use for in vitro ding. € measurements should be discontinued and corrective n taken, OPERATING INSTRUCTIONS ‘The operating instructions for the Models 24/25 Spectro- pphotometers are found in Section Three of Beckman Instruc- tions 015-082245-A, CALIBRATION PROCEDURES ‘The following procedures will enable the operator to verify the performance specifications of the Models 24/25 Spec: trophotometers, Some of the procedures are found in Beckman Instruc tions 015-082245-A, and will be so referenced when applicable. Before proceeding with the verifications of the perform- ance specifications, read Paragraphs 2.6 and 2.6.1 of Beck. man Instructions 015-082245-A. MATERIALS AND EQUIPMENT NEEDED ‘The following materials and equipment are needed to per- form the calibration procedures: 40 mg/l of K.Cr0, in 0.05N KOH for measuring stray light at 370 nm (not supplied with device). 2. 0.25 g/l of K.CrO, in 0.05N KOH for measuring stray light at 370 nm (not supplied with device) 3. O11 g/1 of Nal (aqueous) for measuring stray Tight at 220 nm (not supplied with device) 4, 10 g/l of Naf (aqueous) for measuring stray light at 230 nm (not supplied with device). 5. The 579081 Recorder to measure resolution of the Model 25 Spectrophotometer (supplied if purchased). 6, Holmium oxide filter for measuring resolution of the “Model 24 Spectrophotometer (supplied with device). WAVELENGTH ACCURACY AND REPEATABILITY. CALIBRATION ‘Use procedures outlined in Paragraph 2.6.2 of Beckman In- structions 015-082245-A to vertify this specificationSTRAY LIGHT CALIBRATION The procedure outlined in Paragraph 2.6.5 in Beckman Tn- structions 015-082245-A, allows the operator to check for less than 1% stray light. In order to cheek for stray Tight of less than (1 stated in the specifications, use the following procedure: |. Depress ABS Pushbutton and place Slit Program Selector inthe NORMAL position (Model 25 only) 2. Adjust Wavelength Selector Knob until WAVELENGTH Indicator reads wavelength where measurements are be- ing made. 3, Select tungsten source for check at 370 nm. Select deu- terium source for check at 220 nm. 4, With both the sample and reference beams clear, adjust BASELINE ADIUST Control until the Digital Display reads 0.000. 5, For measurement at 370 nm: a4 Place a I-om cuvette containing 2 solution of 40 ma/] ‘ei K.Cr0, in 0.05N KOH in the reference beam. This will provide a baseline, which means the sample range ‘on the speetrophotometer is approximately 1 10 3 A at 370 am while the Digital Display and recorder range stil 0t02 A b, Place a 1-em eell containing a solution of 0.25 g/l of K.Cr0, in 0.08N KOH in the sample beam, ©. Proceed to Step 7, 6, For measurement at 220 nm: a. Place a J-em cell containing 0.01 1 2/1 Nal (aqueous) in the reference beam. This will provide an approxi- imate 1 to 3 A (at 220 nm) sample range on the spec- traphotometer while the recorder and Digital Display arestill 0102 A, 1 Place a I-cm cell containing a solution of 10 g/l Nal (aqueous) inthe sample beam, «, Proceed to Step 7. 7. Record the absorbance reading ‘8. The absorbance reading for both of the samples should be above 2,000; corresponding to less than 0.1% stray light at cach wavelength, Ifthe device does not display a value above 1.7 A, contact your local Beckman Field Service Representative for assistance. A reading of 1.7 A corresponds to 0.2% stray light which is considered the limit for serious degradation of performance for most in vitro diagnostic assays RESOLUTION Model 25 Only ‘The following procedure is for verification of the resolution specification for the Model 25. (A recorder is required to obtain verification.) Place UV Source Switch in the ON position. Place the Souree Selector in the UV position Depress the ABS Pushbutton |. Place the Mode Switch in the SB position, ‘Adjust the Wavelength Adjust Knob until the WAVE LENGTH Indicator reads 656 nm. 6, Place Slit Program Selector in MANUAL position and adjust MANUAL ADIUST Knob to obtain a slitwidth 9 0.075 nm. 7. Very slowly adjust the Wavelength Selector Knob be- tween 657 and 655 nm until the lowest possible absorb tunce level, without going below 0.000 A, ean be ob: tained. Repeat this operation until satisfied that the low ext absorbance level has been reached 8. IF 0.000 A (or approximately 0 A) cannot be obtained initially, adjust the SB BASELINE Control to obtain a dynode voltage where 0 A can be obisined as described in Step 7, immediately preceding 9, Depress 2.A SPAN Pushbutton, S nm/min WAVE- LENGTH SCAN Pushbutton, and § in/min CHART Speed Pushbutton on recorder. Also depress the Wave- Tength DRIVE Pushbutto 10, Sean the 660 to 655 nm range. The absorbance profile will be a reverse absorbance curve with a peak at ap- proximately 656 nm, At a point 0.3 absorbance um {50% transmittance) from the minimum absorbance, measure the bandwidth, This bandwidth must be les than 0.2 nm to meet specifications. If value larger then 0.2 nm is obtained, contact your local Beckman Field Service Representative for assistance Model 24 Only ‘The following procedure is used for verification of the reso- lution specification of the Model 24: Place a holmium oxide filter in the sample beam, Place Source Selector in VISIBLE position, Depress ABS Pushbution, Place Mode Switch in the DB position. Adjust Wavelength Adjust Knob until WAVELENGTH Indicator reads 470.nm, Depress 2 A SPAN Pushbutton, 5 am/min WAVE: LENGTH SCAN Pushbutton, and 1 in/min CHART Speed Pushbutton. Also depress Wavelength DRIVE Pushbutton, 7. Sean from 470 to 446 nm. In order for the resolution to be better than 4 om, the peak at approximately 453 nm must be two small divisions (on 0 to 100-division chart paper or 0,02 A on absorbance chart paper) higher thao the trough which occurs at a slighily higher wavelength If the specification cannot he verified, contact your local Beckman Field Service Representative for assistance. PHOTOMETRIC ACCURACY AND REPEATABILITY Use the procedure outlined in Paragraphs 2.6.3 und 2.6.4 ‘of Beckman Instructions 015-032245-A for verification of these specifications. 0.4 LINE Use the following procedure to verify the 0 A line speci fication: (Maximum deviation 30,009 A throughout operating range, including noise.) 1. Depress ABS Pushhution, 2. Place Slit Program Selector in the NORMAL. position (Model 25 only), 3. Sean the wavelength range of the instrument either man- tually or automatically from the high to low wavelength Romtemberthat with the Model25 there isa source change at 350 nm, If the spectrophotometer docs not perform a8specified, contact your local Beckman Field Service Rep- resentative for assistance. BASELINE STABILITY ‘This specification (double-beam better than 0.004 A per hour) is verified as follows: (A recorder is required to obtain verification.) 1. Depress /\ Pushbutton, 2, Place Sit Program Selector in NORMAL position (Model 25 only) 3. Turn on tungsten source and place Source Selector in VISIBLE position. Allow source to warm up for two hours. 4, Adjust Wavelength Selector Knob until WAVELENGTH. Tadicator reads $43 nm. 5, Depress 0.1 A SPAN Pushbutton and 0.1 in/min CHART Speed Pushbutton on recorder. 6, Depress START Pushbutton and allow recorder to run for one hour or more at 0.1 in/min. Ifthe spectrophotom- eter does not perform as specified, contact your local Beekman Field Service Representative for assistance. OPERATIONAL PRECAUTIONS AND LIMITATIONS ‘The following operational precautions and limitations are cited so that the operator may avoid those actions which can damage either device, disrupt an automated series of dete rminations, or adversely affect the validity of a quantitative ‘or qualitative determination 1, READ BECKMAN INSTRUCTIONS 015.082245-4, BEFORE OPERATING DEVICE. Tt is also recommended that Beckman Instructions 015.082058, “An Introduction to Ultraviclet Spestropho- tometzy," by Robert J. Manning, be read. 2. Do not subject either spectrophotometer or recorder to mechanical shock, 3. Use only Beckman-recommended spectrophotometer cells, or equivalent, and use procedures found om Pages 10 and LI of Beckman Bulletin 7685, and Paragraph 4.1 ‘ef Beckman Instructions 015-082245-A, for selection, ‘care, and cleaning of cals. ‘4. When selecting a wavelength, always approach the wave: length from the high side of the scale. This will prevent backlash in the Wavelength Adjust Kaob and, thereby, inaccurate wavelength readings. 5. There is no substitute for careful adherence to the pro- cedures indicated by the test kit manufacturer. ‘The operator should check all outputs from the Models, 24/25 Spectrophotometers with expected results HAZARDS ‘The following hazards are identified for maximum safety of the operator. 1, Do not remove any covers from the Models 24/25 Spe ‘trophotometers, of recorder, other than the lamp housing cover as described in Paragraphs 4.3 and 44 of Bockman Instructions 015-082245-A. These paragraphs describe the replacement of the tungsten and deuterium sources. ‘The equipment inside the other covered areas of the de vices is not operator serviceable and presents electrical shock hazard. Refer servicing to a qualified Beckman Field Service Representative. 2, Paragraphs 2.2.1, 2.2.2, and 2.2.3 are not intended for operator use. When a change from one voltage to another is required as stated in these paragraphs, it ‘must be performed by 2 qualified Beckman Field Service Representative 3. When replacing and aligning the deuterium lamp, the ‘operator is reminded that ultraviolet radiation from this lamp is harmful to the eves. Protective (not necessarily safety) glasses must be worn during the alignment procedure. SERVICE No operator service other than the replacement of the tung sten and deuterium sources (Paragraphs 4.3 and 4.4 of Beck- ‘man Instructions 015-082245-A), and front panel indicator lamps, is recommended. Refer service to a Beckman Field Service Representative. Offices are listed on the inside back ‘cover of Beckman Instructions 015-082245-A. ‘Beckman Instruments, In., Fullerton, CA 92634 July 1974 741089 Printed in U.S.A,mer SS ae ‘Reckman Instructions 015-082245-A MODELS 24/25 1 4 | SPECTROPHOTOMETERS | Ie, ulleron, CA, $2634 August 1975. TS1088" OLSO8248 Potted ih OSA.Writer: A, Price Technical Adviser: E, Bridgeford; L, Clougherty; D. Foster M. Kuo; R. Schmidt; A, Way Art Director: D. Wheeler‘SECTION peppy ut TITLE INTRODUCTION ... ‘Model 25 Spectrophotometer ‘Model 24 Spectrophotometer Recorder .. INSTALLATION AND CHECKOUT | Location «2... ao Instrument Setup |» Hookup For 120 and 240 Operation of the Spectrophotometer . Hookup For 120 and 240 Volt Operation of Recorder... : Hookup of Recorder For 50 and 60 Hz Operation ....... Recorder Connection BCD Connector. PAGE Installation of Silica Windows (Optional Accessory) Checkout . Startup... ‘Wavelength Calibration Checkout Absorbance Checkout Photometric Linearity Checkout... 11 ILLUSTRATIONS FIGURE = a TITLE ‘Model 25 Spectrophotometer « Model 24 Specirophotometer + Recorder «..e.seeseesee Hookup of Spectraphotometer for 1 or 240 Volts Hookup of Recorder Far 120 Volts of 240 Volts . 579054 Chart Lopie and Clock Board - Electrical Connections Silica Windows Installed ia Sample Compartment - Holmium Oxide Filter Wavelength Peaks and Tolerances «+ ‘Holmium Oxide Filter Spectrum : Absorbance Values for SRM 930 Filters . ‘Net Absorbance Values for SRM 931 Filters Chromate Linearity Check Stray Light Test Samples . lls SECTION TITLE PAGE 26.5 Stray Light Checkout 5 THREE OPERATION 6 3.1 Operating Controls.) a 6 3.1 Spectrophotometer Operating Controls <2... 6 3.12 Recorder Operating Controls... . 6 32 Startup. 7 33. Absorbance Measurements 0000000000201 8 33.1 Manual Measurements of Absorbance 5 33.2 Recording Absorbance Versus Wavelength =. 8 33.3. Recording Absorbance Versus Time 9 34° Measurement of Concentration «0.1 34.1 Manual Concentration Measurement 34.2 Recording Concentration Measurements 3.5 Differential Absorbance Measurements . 28 Oporaton With Recorder Zero Supp Cont 2 3.7 Proper Slit Width Selection .. FOUR MAINTENANCE .. 4.1 Care and Cleaning of Silica Windows 42° Chart Paper Replacement 43° Replacing and Aligning Tungsten Lamp - 44 Replacing and Aligning Deuterium Lamp |. -_18 45 Replacing Froat-Panel Indicator Lamps FIVE SELECTED REPLACEMENT PARTS LIST .16 FIGURE TITLE, PAGE 15 Speetrophocometer Operating Contiols ..... 6 16 Recorder Operating Controls «+. | 17 Sample Compartment ...+. 8 18 Chart Expansions in amin. sanets 19 Concentration Ranges Availabie on Digital Display for a Given Absorbance Value .....10 20 Concentration Ranges for Various Span Settings fon the Recorder ve... secs veces veee lO 21 Differential Spectrum u 22 Pallseale Settings Obiainable with Each Span Pushbutton and the A Pushbutton Depressed 12 23 Chart Paper Installation “3 24 Source Compartment Sample Compartment | Cover ewes 25 Rear View of Source Compartment 14 26 Front View of Source Lamps - 1sSPECIFICATIONS MONOCHROMATOR Single monochromator, filtergrati blazed at 250:nm. OPTICAL PRINCIPLE Double-beam and single-beam. WAVELENGTH RANGE Model 24 Spectrophotometer: 340 fo 700 nm, “Model 25 Spectrophotometer: 190 to 700 nm, WAVELENGTH PRESENTATION Linear, digital counter WAVELENGTH ACCURACY 05 nm, WAVELENGTH REPEATABILITY Better than 0.25 nm, STRAY LIGHT “Model 24 Spectrophotometer: less than 0.1% at 370.am, “Model 25 Spectrophotometer: less than 0.1% at 220:am, RESOLUTION Model 24 Spectrophotometer: 4 am. ‘Model 25 Spectrophotometer: 0.2:nm SLITS ‘Model 24 Spoctrophotometer: Fixed, 0.8 mm. Model 25 Spectrophotometer: Two programs for double- beam operation, and manually adjustable 0.05 mm to 2.0 mam, PHOTOMETRIC READOUT Digival Display “4-digit digital display, 0 t0 2 A, oF 0 t0 8000 counts in con- centration, Recorder 100-millivolt per absorbance unit Differential “0.3 Ato $0.7 A with 1A Pushbutton depressed. BCD Connector (to 2.A or 0 to 8000 counts in concentration through 36-pin PHOTOMETRIC ACCURACY 0.5% of reading or 0.001 A, whichever is larger PHOTOMETRIC REPEATABILITY, 0.25% of reading or 0.001 A, whichever is larger. RECORDER SCALES 0.1A,0.25 4,05 A, 1.0 A, and 2.0.8 fullscale SCANNING SPEEDS 100, 50, 20, and 5 nm/min selectable on accessory recorder ALINE ‘Maximum deviation 0.009 A throughout operating cange, including noise, BASELINE STABILITY ‘Double-beam better than 0.0004 A per hour. APPROXIMATE WEIGHT Normal: 70 pounds net. Shipping: 8§ pounds. OVERALL DIMENSIONS 2d inches jong x 16 inches deep x 14 inches high POWER REQUIREMENTS. 120/240 volts (==10% ), 50/60 Hz, 2 amperes.SECTION ONE INTRODUCTION ‘The Models 26 and 25 Spectrophotometers are double-beam, digital reading or recording instruments which can make absorbance, concentration, and differential absorbance meas- lurements. Quantitative and qualitative analysis and reacti rate studies are easly accomplished. Specialized techniques, such as automatic sumpling and batch sampling, are possible with addition of easily mounted accessories |. MODEL 25 SPECTROPHOTOMETER ‘The Model 25 Spectiophotometer, Figure 1, is mounted in @ ‘compact case. Major design features include: two sources (Gungsten and deuterium), power supplies, monochromator detector, large sample compartment, variable sit contro, slit program selector, solidstate cirouitry, and a bull system, 1.2. MODEL 24 SPECTROPHOTOMETER ‘The Model 24 Spectrophotometer, Figure 2, is housed in 2 ‘compact case with front and rear panel controls, and a four

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