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  • Sequence: Oligo Explorer - 1.1.2 10/04/2013

    Diunggah oleh

    Clau¿ R.
    9 tayangan2 halaman
    This document analyzes primers and a PCR product for a sequence called Avesconsenso.txt that is 1143 bp long with 137 bp unresolved. An upper primer of 19 bp is identified at position 241 with a GC% of 52.6% and a lower primer of 19 bp is identified at position 840 also with a GC% of 52.6%. The PCR product would be 600 bp long from positions 241 to 840 with a GC% of 44.5% and Tm of 77C for the product and 56.3C for annealing.

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    This document analyzes primers and a PCR product for a sequence called Avesconsenso.txt that is 1143 bp long with 137 bp unresolved. An upper primer of 19 bp is identified at position 241 with a GC% of 52.6% and a lower primer of 19 bp is identified at position 840 also with a GC% of 52.6%. The PCR product would be 600 bp long from positions 241 to 840 with a GC% of 44.5% and Tm of 77C for the product and 56.3C for annealing.

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    © All Rights Reserved
    Unduh sebagai RTF, PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    9 tayangan2 halaman

    Sequence: Oligo Explorer - 1.1.2 10/04/2013

    Diunggah oleh

    Clau¿ R.
    This document analyzes primers and a PCR product for a sequence called Avesconsenso.txt that is 1143 bp long with 137 bp unresolved. An upper primer of 19 bp is identified at position 241 with a GC% of 52.6% and a lower primer of 19 bp is identified at position 840 also with a GC% of 52.6%. The PCR product would be 600 bp long from positions 241 to 840 with a GC% of 44.5% and Tm of 77C for the product and 56.3C for annealing.

    Hak Cipta:

    © All Rights Reserved
    Unduh sebagai RTF, PDF, TXT atau baca online dari Scribd
    Tandai sebagai konten tidak pantas
    dari 2

    Oligo Explorer - 1.1.

    2 10/04/2013

    Sequence

    Filename : Avesconsenso.txt
    Length : 1143 bp
    Unresolved : 137 bp
    GC % : 43,7 %

    Upper primer
    5'-position : 241
    Length : 19
    Sequence : 5´-CGNAACCTNCACGCAAACG-3´
    3´-GCAAACGCACNTCCAANGC-5´

    Tm (basic) : 58,0 ºC
    Tm (salt) : 57,3 ºC
    Tm (NN) : 58,1 ºC

    GC % : 52,6 %
    3´-tail GC % : 57,1 %
    dG : -32,5 kcal/mol
    3´-tail dG : -14,0 kCal/mol

    Lower primer
    5'-position : 840
    Length : 19
    Sequence : 5´-GGCGTAHGCGAATAGGAAG-3´
    3´-GAAGGATAAGCGHATGCGG-5´

    Tm (basic) : 58,0 ºC
    Tm (salt) : 57,3 ºC
    Tm (NN) : 57,3 ºC

    GC % : 52,6 %
    3´-tail GC % : 42,9 %
    dG : -34,7 kcal/mol
    3´-tail dG : -10,8 kCal/mol

    Primer pair properties


    Length difference : 0
    Tm (basic) difference: 0,0 °C
    Tm (salt) difference : 0,0 ºC
    Tm (NN) difference : 0,9 °C
    GC % difference : 0,0 %

    PCR product
    Position : 241 - 840
    Length : 600 bp
    GC% : 44,5 %

    Tm(product) : 77,0 ºC
    Tm(annealing) : 56,3 ºC

    Upper primer self annealing:


    None!
    Upper primer loops:
    None!

    Lower primer self annealing:


    None!

    Lower primer loops:


    None!

    Upper primer - Lower primer annealing:


    5'-CGNAACCTNCACGCAAACG-3'
    : : ||||
    3'-GAAGGATAAGCGHATGCGG-5'
    dG: -4,89 kcal/mol

    5'-CGNAACCTNCACGCAAACG-3'
    ::: ||| :::
    3'-GAAGGATAAGCGHATGCGG-5'
    dG: -3,54 kcal/mol

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