Constanza Olguin
Partner: Huaning Li
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Introduction:
Ascorbic acid, most commonly known as Vitamin C, is an antioxidant that is essential for
human nutrition. Vitamin C is synthesized from glucose in the liver of most mammalian
species, but not by humans.1 This is why humans must ingest Vitamin C in their diets,
appropriately treated could be lethal. According to Zhang, this vitamin was first synthesized
Due to its presence in a variety of fruits and vegetables, vitamin C is available for
consumption in form of fruits and vegetables, and also as a supplement in all industrialized
countries.1 Plenty of studies have shown that the amount of ascorbic acid present in a
variety of samples it has been different every time and this is because ascorbic acid is easily
oxidized. There are several storage conditions that can affect the degradation of vitamin C
tablets like temperature and humidity.3 Because of its easy degradation when manufacturing
supplements of vitamin C it is very important to take into account how quickly it degrades
so that the amount and the expiration dates can be responsibly reported in the containers.
This lab involves the analysis of a sample of Vitamin C to determine the moles of Vitamin
C. A way to analyze the vitamin C relies on iodometry in the presence of a starch indicator.
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In this reaction, iodine and vitamin C react in a stoichiometric ratio of 1:1. In this
this the solution will turn blue-black in colour and the excess amount of iodine can be now
titrated against standardize sodium thiosulfate. This type of titration is called back-titration
and is used to determine the moles of vitamin C by subtracting the leftover moles of I2 with
Procedure:
The procedure given in the First Year Chemistry Lab Manual for Experiment G4 was
100 ml of distilled water stirring it using a plate and magnetic stir bar. After being
through a gravity filtration set up, washing the beaker and filter paper with small
amounts of distilled water, and then fill up to the mark with distilled water. The solution
A solution of diluted KIO3 of 250ml was made from the concentrated solution of KIO3
5*10-5 M.
solution into a 125 mL Erlenmeyer flask. To the Erlenmeyer flask, 0.2 g of KI, 1 mL of
1M sulfuric acid was added and 10ml of Vitamin C solution. The titration was carried
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out until a pale yellow colour was obtained. After that 5 drops of starch solution were
added and the titration was continued until the blue colour just disappeared. This was
Observations:
First the solution had a raspberry color, when the first part of the titration was added it
turned pale yellow. Upon the addition of the starch solution it turned into a blue color,
and the titration was continued. As the endpoint is approached, it was important to titrate
slowly with swirling. At the endpoint of the titration, the color of the solution
disappeared.
See the attached Lab K Report Sheets for Data and Calculations.
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Discussion:
Through the technique of back-titration, the moles of Vitamin C of the sample was
determined. A solution of Vitamin C was made and with the added starch indicator was
titrated with sodium thiosulfate to determine the moles of vitamin C by knowing the
In this titration, the starch indicator changes color depending on whether or not it is
bound to Iodine ions present in the solution. The starch is in the when is added to the
solution turns it from a pale yellow color to a blue color, when the titration is at its end
point the starch will change and the color will disappear, indicating the completion of the
titration.
The sample of Vitamin C required 15.41 ± 0.04 ml of Sodium thiosulfate to reach the
end point. This means that the numbers of moles of I2 used by the Vitamin C solution
with I2 in 10 ml is the same. The mass in mg of the original tablet of vitamin C was then
There is an uncertainty associated with each of the measurements taken in the lab, which
is propagated into an uncertainty in the final calculated values of the mass of vitamin C
in the original tablet (± 1.019*10-9 mg). This uncertainty is associated with the dilution
of the KIO3 solution, the uncertainty associated with the sodium thiosulfate solution, and
the titration of the sample using different types of glassware, including the burette,
which has an error of 0.02 mL per reading. Through the multiple readings taken
throughout the process of the titration, the errors begin to accumulate resulting in the
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The final mass of vitamin C in the tablet determined by the calculations was higher than
the one stated in the package, and it is reasonable taking into account that vitamin C
degrades easily. It would be reasonable that manufacturers would add more vitamin C
that the one stated in the package to lengthen the effectivity of the product and because
the package is supposed to expire in 2019 this would make the product have 500mg or
Conclusion:
The well water was determined to have a mass of 509.9 ± 1.019*10-9 mg, which is it
reasonable due to vitamin C’s fast degradation and its expire date on 2019. The
uncertainty in the measurement of ± 1.019*10-9 is very low, indicating that the technique
is precise.
References:
[1] Padayatty, S. J. Journal of the American College of Nutrition. 2003, vol. 22, p 18-35.
https://www.livestrong.com/article/463760-do-vitamin-c-pills-go-bad-or-lose-potency/
[4] First Year Chemistry Lab Manual: Chem 111/113 & Chem 121/123, University of