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SLE234 Microbiology Laboratory Report 1 2018

Please answer the questions in the text boxes, short answers and dot points are fine.

1. Describe the difference between selective and differential media. Give an example of each.

(3 Marks)

2. Can you suggest a method, other than the streaking-out technique, by which a single
bacterial colony could be isolated?

(2 Marks)

3. Give two reasons why you incubate your plates in an inverted position?

(2 Marks)
SLE234 Microbiology Laboratory Report 1 2018

4. Match the terminology correctly with the definition in the table below (eg A=1)

TERMINOLOGY DEFINITION
A. Aerosol 1. Disinfection or sterilization of contaminated
articles
B. Airborne infection 2. Process to kill or remove pathogenic
microorganism (not spores)
C. Asepsis 3. Caused by inhaling airborne material carrying
microbial contaminants
D. Decontamination 4. Process to kill/remove all microorganisms
including spores and viruses
E. Pathogen 5. Thick walled resting cells formed by G + org.
F. Infection 6. Organism able to cause disease in a host
G. Disinfection 7. Suspension in air of finely dispersed solids or
liquids
H. Viable microorganisms 8. Organism capable of multiplication
I. Sterilization 9. Prevention of microbial contamination
J. Spores (bacterial) 10. Disease caused by pathogen transfer

Terminology Definition
A
B
C
D
E
F
G
H
I
J

List 3 Agents used for STERILIZATION List 3 Agents used for DISINFECTION

(16 Marks)
SLE234 Microbiology Laboratory Report 1 2018

5. What reason could there be for failing to obtain isolated colonies on a streak plate?

(2 Marks)

6. List at least three different media or procedures used to grow anaerobic bacteria.

(3 Marks)

7. What is a bacterial colony?

(2 Marks)

(Total= 30 Marks)