THE POWER OF

EASY & AFFORDABLE

FLOW CY TOMETRY & CELL COUNTING
 THE POWER OF
EASY & AFFORDABLE
FLOW CY TOMETRY
& CELL COUNTING

Many assays. The desire to gain a systems perspective by analyzing whole cells has
One platform.
fueled a rising interest in cell- and bead-based applications for basic,
Direct, absolute cell
counts and viability applied, and clinical life science research, including drug discovery
Apoptosis and development efforts. By studying intact cells, researchers can
• Annexin V
• TUNEL gain critical insights into the interactions among biological pathways.
• Pan caspase Further, they can leverage cell studies to expedite target validation, lead
• Caspases 3/7 and 8
• Mitochondrial membrane potential optimization, process development, toxicity determinations, clinical
GFP expression trials, and much more.
Cell cycle
Antigen detection Accelerate discovery and increase productivity.
Cell toxicity Guava® Assays can be applied to a wide and ever-expanding range of applications.
Cell proliferation Cell counts and viability Cell cycle
Cell painting • Cytotoxicity screening • Cell line characterization
• Cell culture optimization • Compound screening
Cell signaling • Bioprocess monitoring • Bioprocess monitoring
Antibody quantification
Apoptosis Cell tracking
• Cell line characterization • In vitro and in vivo partitioning
Multiple cell types.
• Compound screening • Cell toxicity
• Transformed • Mammalian • Bioprocess monitoring • Cell proliferation
• Primary • Insect
Antigen detection Fluorescent proteins
• Suspension • Yeast
• Compound profiling • GFP screening
• Adherent
• Protein/antigen expression analysis • Combined transfection efficiency and viability
• Hybridoma screening Bead-based assays
Beads.
• CD marker detection • Antibody quantification
• Cytokine quantification

2
“[Guava® Systems] have great value not only for assay development and drug lead optimization,
but also as credible screening instruments giving high content readouts from a single well.”
—Jill Gregory, Staff Scientist, Genzyme Corporation
Drug Discovery

Flow cytometry on demand. Do more. Guava® PCA System

The Guava® PCA and PCA-96 Systems as well
as the Guava EasyCyte™ Mini and Plus
Systems have been designed to meet the needs
of today’s life scientist. These on-demand
systems, together with an ever-growing suite of
assays, deliver powerful solutions for counting,
profiling, and screening a most valuable
commodity—your samples. Whether you focus
on scientific applications, routine screening, cell
counting, or commercial production, Guava
Technologies has the solution that is right for
you.
Turnkey solutions.
Guava Technologies has streamlined and simpli-
fied fluorescent cell- and bead-based assays by
integrating instrument, software, and reagents
into targeted, turnkey solutions. Samples in.
Answers out. Next study. The assay develop-
ment has been done for you, so you can
concentrate on getting results.
Four systems meet a variety of needs.
Each Guava® Assay meets exacting standards of
intuitive
robustness, ease-of-use, and affordability, software
while requiring only a few microliters of sample. interface
And, Guava Systems are designed to be very easy
to use, so you can be up and running within a
few hours! Sophisticated cell analysis has never Green
been easier. laser
MINIMAL
Do it right. WASTE
Guava Systems combine fluorescence-based
techniques and high quality optical elements to Guava® PCA-96 System
maximize assay sensitivity. And, automated
software routines reduce operator-induced
variability. The result? Accuracy and reproduc-
ibility.
Do it fast.
With an integrated system of instrument, software,
and reagents from Guava Technologies, you don’t
have to rely on core labs to run your sophisticated
cell analysis assays any longer. You can conduct your
own studies in your own lab on your own schedule. Microplate
Guava EasyCyte™ Mini System or tubEs

PCA PCA-96 EasyCyte Mini EasyCyte Plus

Laser Green Green Blue Blue

OPTIONAL:
- side scatter
Tiny footprint    

SCATTER
Forward (FSC)    
BLUE
Side (SSC) ­
— ­
— optional optional laser

Tube sampling    
Automated 96-well Guava EasyCyte™ Plus System
microplate sampling —  — 
OPTIONAL:
Small sample size
(<20 µL)     - side scatter
- 4 th color detector
Minimal waste
(<50 mL per day)    

Green — —  
FLUORESCENCE Yellow    
DETECTORS Red    
Near infrared — — — optional

3
 THE POWER OF CHOICE.
M A N Y A S S AY S . O N E P L AT F O R M .

Guava Technologies has streamlined and simplified cell- and Cell cycle.
Determining the distribution of cells across the
bead-based assays by integrating instruments and software phases of cellular replication and division is
with optimized reagents. Now, you can conduct cell analysis done easily with the Guava® Cell Cycle Assay.
In addition to providing G0/G1, S, and G2/M
studies from your bench top, on your schedule. Now, you phase percentages, this assay exports data in
have the ability to generate data wherever and whenever industry standard formats to allow more
detailed analysis. M

30 you want to make better, faster decisions and maximize your 100
G2

laboratory’s productivity.
G0
G1

75
S

Cell Count
Culture Endpoint analysis
50
cells Guava® Cell Cycle Assay

Guava® Caspase 3/7 Apoptosis Assay The Guava EasyCyte Plus System 25
Induce offers a convenient platform
Guava® Caspase 8 Apoptosis Assay for exploring apoptosis biology,
while addressing important 0
Split cells applications such as endpoint 0 1024 2048 3071 4095
Guava® Multi-Caspase Apoptosis Assay
& select analysis and mechanism of DNA Content (PM2)
desired Guava Nexin® Annexin V Apoptosis Assay action studies.
assays
Guava® Mitochondrial Depolarization Apoptosis Assay
Multicolor antigen detection.
Guava® TUNEL Apoptosis Assay
Guava Express® ss® Cell Surface Antibodies are
Mechanism of action
fluorophore-conjugated antibodies that have
been optimized for use on most Guava®
Cell counting and viability. Accuracy Systems. The antibodies provide quick, easy
Speed, ease, and depth: Unlike other viability Based on the detection of fluorescent DNA-
100%
staining of human CD markers and allow for
assays, the Guava ViaCount® Assay not only binding dyes, the ViaCount Assay offers far rapid multicolor immunophenotyping of
Observed Viability

95%
detects viable and dead cells, but also simul- greater accuracy and reproducibility than hematopoetic cells. The Guava Express® Assay
taneously assesses the apoptotic cell fraction. traditional
90% Trypan Blue-based methods, while and Guava Express® Plus and Pro Software
requiring only a single reagent, it is a simple no- using a much smaller cell sample. Because it Modules allow you to use your own primary
85%
conjugates or our second-step reagents to
Guava ViaCount
wash, mix-and-read assay that rivals the fastest can identify both viable and apoptotic
Trypan Blue/ cells,
Hemacytometer
of similar assays. Moreover, its versatility extends the ViaCount
80% Assay can be used for cytotoxicity detect surface and internal receptors or antigens
its applicability across a wide range of cell types, screens, 80%
proliferation
85%
assays,
90%
and cell
95%
culture
100% on single cells. This is ideal for hybridoma-
Expected Viability
including suspended and adherent, mammalian monitoring. In addition, ViaCount reagents can screening, immunophenotyping, and other 2-,
and non-mammalian, and many others. assess cell health in any cell-based assay. 3-, or 4-color assays.
Accuracy Reproducibility 10e4

100%
CD45RA-PE (YLW-HLog)
Low Viability

Guava ViaCount
Assay 10e3
Observed Viability

95%
Trypan Blue
10e2
90%
Low Cell Density

Guava ViaCount
Assay
85% Guava ViaCount
10e1
Trypan Blue/ Trypan Blue
Hemacytometer
80% 10e0
80% 85% 90% 95% 100% 0 5 10 15 20 25 30 10e0 10e1 10e2 10e3 10e4
Expected Viability Percent CV CD3-PECy5 (RED-HLog)

At lower viability levels, the Guava ViaCount Assay provides more As shown above, the Guava ViaCount Assay provides superior Dual staining of lysed whole blood with anti-CD45rA-PE and
accurate assessments of cell culture viability than Trypan Blue dye reproducibility over manual cell counting with Trypan Blue in cell anti-CD3-PECy5 identifies naïve T cells (CD3+CD45rA+), memory
exclusion assays using a hemacytometer. cultures with low viabilities or low cell concentrations. T cells (CD3+CD45rA-), and B cells (CD3-CD45rA+). Cells in the
Reproducibility lymphocyte gate were used for this analysis.
4
ility

Guava ViaCount
Assay
“The Guava® PCA is a major advance in our ability to rapidly and easily measure most
of the parameters important in cell biology.” —Jennie Mather, CEO and President, Raven Biotechnologies, Inc.
Cancer Therapeutics

Apoptosis. Caspases. By virtue of fluorochrome- Mitochondrial membrane potential.

conjugated caspase inhibitors in combination Designed to save time and effort, the Guava®
In a process as complex as apoptosis, multiple
with a viability dye, Guava® Caspase Assays Mitochondrial Depolarization Assay is a no-
methods of assessment are essential to identify
detect the activation of various caspases during wash, mix-and-read assay to monitor changes in
cell populations
the apoptotic process. Up to four separate mitochondrial membrane potential (∆ψ), the loss
in various stages
populations (viable, early-to-mid apoptotic, late of which is often associated with early apoptosis.
of cell death. The
apoptotic, and dead) can be resolved in a single Collapse of this potential may coincide with
Guava® Apoptosis
assay. These assays detect simultaneous the opening of the mitochondrial permeability
Assay Suite
activation of multiple caspases, caspase 3/7, or transition pores, leading to the release of
contains several
caspase 8. cytochrome C into the cytosol which triggers
complementary
downstream events in the apoptotic cascade.
assays that can E
Addition of the dead cell dye, 7‑AAD, allows you
all be done on a
to easily discern healthy, polarized cells from the
single platform, E
apoptotic/depolarized and dead cells.
 !!$0-

quickly and easily.

E

Fluorescence Emission

Orange JC-1 Aggregates

Green JC-1 Monomers
E

E
Annexin V. Designed to save time and effort, E E E E E
32 6!$ &-+0-
the Guava Nexin® Assay is a no-wash, mix-
Up to four cell populations (viable, early-to-mid apoptotic, late
and-read assay to identify cells in early- to mid- apoptotic, and dead) can be detected using tilting markers 450 500 550 600 650
stage apoptosis. It is one of the fastest and in the Guava® Multi-Caspase Assay. Wavelength (nm)
easiest apoptosis assays available. Using The Guava Mitochondrial Depolarization Assay can distinguish
Annexin V conjugated to a fluorophore, this depolarized Jurkat cells, which fluoresce mostly green, from healthy

assay detects the transfer of phosphatidyl serine TUNEL. This classic assay for apoptosis-induced uninduced cells, which fluoresce mostly orange (overlay).

from the internal to the external face of the DNA fragmentation has been reworked to make
it more convenient and accessible. The Guava® Fluorescent proteins.
plasma membrane during the initial stages of
apoptosis. Addition of the dead cell dye, 7‑AAD, TUNEL Assay detects late-stage apoptotic cells Screening clones of Green Fluorescent Protein
allows you to easily discern early apoptotic cells by using sensitive fluorescent probes to detect (GFP) transfectants is now quick and easy:
from late apoptotic /dead cells. DNA strand breaks. Guava Express® Plus or Pro Assays automate
single cell GFP detection in 96-well plates and
Induction of Early to Mid-Apoptotic Cells
combine it with viability assessment, apoptosis,
80 5-Azacytidine
80
or detection of a second (Plus) or third (Pro)
Ellagic Acid marker. The Guava Express Plus or Pro Assays
60 Mebendazole 60
eliminate the need to test each clone individually
% Annexin V PE+

Albendazole
since an entire plate of transfectants can be
Cell Count

Helenine
40
40 analyzed in a single experiment.
20 20
Guava Express Plus & Pro
Assays make screening for
0 0 Green Fluorescent Protein
10 -2 10 -1 10 0 10 1 10e0 10e1 10e2 10e3 10e4
Compound Concentration (µM)
(GFP) and other fluorescent
DNA Strand Breaks (PM1)
proteins fast and easy.
Compound dose response curve on Jurkat cells. Jurkat cells The Guava TUNEL Assay easily distinguishes and quantifies the
were treated with the indicated compounds at a range of apoptotic cell population, as seen in this sample of HL60 cells
concentrations for 24 hours before testing for apoptosis treated with 20 µM camptothecin for 4 hours.
induction using the Guava Nexin Assay.
5
 “The beauty of the Guava® PCA System is that it generates reliable and reproducible
quantification of apoptotic cells.” —Maureen E. Murphy, Ph.D., Department of Pharmacology, Fox Chase Cancer Center
Cancer Research

Cell tracking. Hybridoma screening. Accurate Prediction of Human IgG Concentrations

20
Improve your cell tracking with three easy-to- Using quantitative bead-based measurements, Guava

Observed Concentration (µg/mL)

ELISA
use, non-radioactive assays that are superior to Guava RapidQuant™ IgG Assays quantify the Expected Conc.
15
population-based methods. Cell tracking assays levels of soluble IgG in hybridoma supernatants
from Guava Technologies use a well-character- with much less work than traditional assays, 10
ized cell tracking dye, CFSE, that diffuses freely such as ELISAs. Designed to be easy and
into cells and is retained within the cell without effortless, rapidQuant IgG Assays feature no- 5

affecting cellular function. wash protocols, superb day-to-day reproduc-

Guava Cell Toxicity Assay ibility, shorter time to 0
0 5 10 15
Cytotoxicity. When CFSE is answer, and improved Expected Concentration (µg/mL), A280
used in combination with accuracy over other Dilutions of various antibodies tested with the Guava rapidQuant
the dead cell dye, 7-AAD, all available assays. Both Human IgG Assay show excellent correlation with predicted
values from ELISAs.
of the commonly performed mouse and human
cytotoxicity effector assays
Dedicated software.
rapidQuant IgG Assays
(NK, CMC, ADCC) can be are universal—they The friendly and intuitive Guava CytoSoft™
performed with the quantify all IgG subtypes Operating Environment and Guava®
Guava® Cell Toxicity Cytotoxicity data are automatically analyzed and displayed in
of that species. Further, Assay-Specific Software Modules guide
an easy-to-read format.
Assay. rapidQuant Assays can you through setup, maintenance, cleaning, and
be combined with other assays from Guava shutdown procedures as well as data acquisi-
Cell proliferation. The Guava® Cell Growth tion and analysis. All modules are designed
Technologies to accelerate hybridoma screening
Assay is used to monitor cell proliferation of to streamline the experimental process from
and monoclonal antibody development by
primary cultures out to five generations as well hypothesis to conclusion. results can be
allowing all stages of testing to be performed
as to discern which portion of the cells are dead exported to spreadsheets or as industry-stan-
from less than 50 µL of supernatant.
when stained with propidium iodide. dard FCS 2.0 or 3.0 files for further analysis by
Guava Cell Growth Assay
Optimized reagents. third-party software. And, all software modules
support compliance with 21 CFR Part 11 regu-
Optimized for use on all Guava® Systems,
lations. For multicolor detection, the Guava
reagents from Guava Technologies are formu-
Express® Pro Software Module allows you
lated to ensure excellent
to view and analyze up to eight user-selectable
sensitivity and
histogram or dot plots simultaneously. You
ease-of-use. These
can also customize axis labels and statistics,
unique reagents
and select multiple marker and gate types.
can also improve
Additional features include histogram overlays,
and accelerate your
backgating, zoom-to-plot for enlarged views,
assays by eliminat-
and batch-file printing.
Enriched T cells stimlulated with 5 µg/mL PHA, assayed on day 4 ing many of the common preparation steps
and analyzed using the ModFit™ software package from Verity required with traditional proto-
Software House.
cols. In some cases, samples can
Cell painting. The Guava® Cell Paint Assay be read immediately after stain-
utilizes the cell tracking dye as a single color ing, with no loss of sensitivity.
assay to track target cells within a mixed
population for in vivo and in vitro applications.
A second, user-defined dye can be added to
perform a dual color assay for monitoring
protein expression or antigen specificity.
Guava Express® Pro Software.

6
THE POWER OF SMALL.
T H E G UAVA F O R M U L A .

Turnkey solutions for cell analysis from Guava Technologies are

based on a superior interplay among reagents, software, and
instrument. This sophistication elevates cell analysis and counting to
an art—while bringing it down to earth. The result: Tools that are
truly a pleasure to use.

The technology behind it all.

minimal waste (<50 mL per 8 hours), saving
So how does so much power and functionality
you both time and money. Also, since the flow
get packed into such a small space? Credit a
cell is self-aligning and user-replaceable,
unique design that optimizes every available bit
you can remove it at any time for cleaning and
of space and eliminates wasted corners. Then,
maintenance yourself—no more expense or
credit a unique microcapillary flow cell
downtime for service visits. And, of course, the
technology that eliminates the requirement for
tiny footprint fits into the tightest spots,
complicated sheath flow fluidics. The end result?
reserving valuable laboratory bench space for
An extremely compact, easy to use, and easy to
other equipment and uses.
maintain system, supporting on-demand cell
analysis. Sheathless fluidics translates into The flow cell is easily
fewer cells (<20 µL), less reagent, and removed for routine
maintenance.

Guava® PCA System

photomultipliers (PMT)
detect fluoresecently
labeled cells

Guava EasyCyte™ Plus System

SIDE-scatter (sSC)
photoMULTIPLIER (PMT)
Differentiates cells
by GR ANULARIT Y

Forward scatter
(FSC) photo diode
GREEN laser excites
Differentiates
fluorescently
cells by size
labeled cells

At the heart of Guava Systems is a patented, microcapillary, laser-based technology

capable of detecting mammalian and microbial cells and beads. A sample of
fluorescently labeled cells is aspirated into a uniquely proportioned microcapillary
flow cell. A green or blue diode laser excites the cells—each cell emits signals that blue laser excites
are individually detected by photomultipliers and a photo diode. Guava Software
fluorescently
Modules show all relevant data and results immediately.
labeled cells

7
Expert support and service. Specifications PCA PCA-96 EasyCyte Mini EasyCyte Plus
Excitation 532 nm 532 nm 488 nm 488 nm
All products from Guava Technologies are
Emission 580/20 nm 580/20 nm 525/30 nm 525/30 nm
designed to increase productivity, maximize 675/20 nm 675/20 nm 583/26 nm 583/26 nm
680/30 nm 680/30 nm
uptime, and minimize maintenance. But, 785/70 nm (optional)
in the rare instance when you do need Light scatter Forward Forward Forward Forward
Side (optional) Side (optional)
assistance, our customer solutions team Dimensions 8.5 in (21.6 cm) h 13 in (33 cm) h 9 in (23 cm) h 13 in (33 cm) h
is always available to 12.6 in (32 cm) w 16 in (40.6 cm) w 15.5 in (39.4 cm) w 16 in (40.6 cm) w
14.3 in (36.3 cm) d 22.5 in (57.2 cm) d 17.9 in (45.5 cm) d 22.5 in (57.2 cm) d
answer questions about Weight 32 lbs (13.2 kg) 80 lbs (35.6 kg) 50 lbs (22.7 kg) 80 lbs (35.6 kg)
our software, instru- w/laptop w/laptop w/out monitor w/laptop
Sample format 1.2 & 1.5 mL Tubes 0.5 mL Tubes 1.2 & 1.5 mL Tubes 0.5 mL Tubes
ments, reagents, and 96-Well Plates 96-Well Plates
applications. Individual Applications
members of the team
Specialty
specialize in differ- Guava ViaCount® Assay
   
ent disciplines of life Cell counting and viability
Guava® Cell Cycle Assay
science research to ensure your questions G0/G1, S, G2/M phase distribution    

are answered quickly, thoroughly, and Guava Express® Cell Surface Antibodies1

2

2
 
Antigen detection
completely the first time you call. Guava RapidQuant™ IgG Assays1
Mouse & human IgG quantification n/a n/a n/a 

Get started today. Apoptosis

Guava Nexin® Assay
Discover how products and services from Annexin V binding    

Guava Technologies can accelerate discovery Guava® Caspase 3/7 Assay1

Caspase 3/7 activation n/a n/a 
3

and increase the productivity of your Guava® Caspase 8 Assay1 3
Caspase 8 Activation n/a n/a  
organization. For more information, contact Guava® Multi-Caspase Assay
us directly or visit: Pan caspase    
Guava® TUNEL Assay
www.guavatechnologies.com DNA fragmentation    
Guava® Mitochondrial Depolarization Assay
Mitochondrial membrane potential n/a n/a  

Cell tracking
Guava® Cell Toxicity Assay
Cytotoxicity: NK, CMC, ADCC    
Guava® Cell Paint Assay
Target cell monitoring    
Guava® Cell Growth Assay
Cell proliferation: Primary cultures n/a n/a  
1 Requires Guava Express®, Express® Plus, or Express® Pro Software Modules.
2 Users must optimize Guava Express Cell Surface Antibodies for the PCA and PCA-96 Systems.
3 Users may transfer prepared samples to tubes for collection on the EasyCyte Mini System.

Optional software
Guava Express® Software Module (2-color)
Antigen detection; immmunophenotyping   n/a n/a
Guava Express® Plus Software Module (3-color)
Antigen detection, GFP, immunophenotyping, n/a n/a  
antibody quantification
Guava Express® Pro Software Module (4-color)
Antigen detection, GFP, immunophenotyping, n/a n/a n/a 
antibody quantification, cell signaling

Global Headquarters Europe & Africa Asia-Pacific

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25801 Industrial Blvd. 3 Southview Business Centre­ 1502, DLF Galleria, DLF Phase 4
MK1007-03 Mar07 5K

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Tel: 1 510 576 1400 Stamford, Lincs PE9 2JL UK Haryana, India
Fax: 1 510 576 1500 Tel: +44 (0) 1780 764390 Tel: +91 124 4148247 For Research Use Only. Not for use in diagnostic procedures. ModFit is a trademark of Verity
www.guavatechnologies.com Fax: +44 (0) 1780 765322 Fax: +91 124 4148504 Software House. Guava, Guava Technologies, the Guava Logo and all other trademarks are the
info@guavatechnologies.com sales@guavatechnologies.com info-ap@guavatechnologies.com property of Guava Technologies, Inc. ©2007 Guava Technologies, Inc. All rights reserved.