Student number: 12018448 Advanced DNA Examination 9th January 2018

Table 1. MtDNA

Position within Hypervariable 1 region of mtDNA

HV1

15974
16007
16044
16086
16093
16131
16144
16152
16178
16188
16230
16277
16336
16367
16369
16378
Origin of Sample Source
HV1 A A - G A G C C C C A G A C C C
mtA G A - G A G C A A G A G A T C C C
mtB A A - G T A G C C C - C A C C A T
mtC A C A C A G C C C C A G T C G C G
mtD G A - G A G C A A G A G A T C C A
mtE A A - G T A G C C C - C A C C A
mtF A C A C A G C C C C A G T C G C

Table 2. SGM Plus profiles

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 1 14,16 16,19 10,14 20,23 X 13 29,33 16,18 12,14 6,9.3 22,23
Profile 2 15,16 15,19 - 18,20 XY 12,13 29 16,20 13,14 7,9.3 -
Profile 3 15,16 18,19 10,11 19,22 X 13 28,29 12,16 12,15.2 9.3 19,22
Profile 4 16 16,19 11,14 20 XY 10,13 28,29 13.2,20 14,15 7 23,25
Profile 5 15 15,20 12,13 17,18 X 11,12 29,33 15,16 13,15 7,9.3 25,26

Table 3. Potential match

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 1 14,15 16,18 10,14 20,23 X 13 29,33 16,18 12,14 6,9.3 22,23
Profile 2 15,16 15,19 - 18,20 XY 12,13 29 16,20 13,14 7,9.3 -
Profile 4 15 16,19 11,14 20 XY 10,13 28,29 13.2,20 14,15 7 23,25

Table 4. Potential match

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 1 14,16 16,18 10,14 19,23 X 13 29,33 16,18 12,14 6,9.3 22,23
Profile 3 15,16 18,19 10,11 19,22 X 13 28,29 12,16 12,15.2 9.3 19,22
Profile 4 16 16,19 11,14 20 XY 10,13 28,29 13.2,20 14,15 7 23,25
Student number: 12018448 Advanced DNA Examination 9th January 2018

Table 5. All female

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 1 14,16 16,19 10,14 20,23 X 13 29,33 16,18 12,14 6,9.3 22,23
Profile 3 15,16 18,19 10,11 19,22 X 13 28,29 12,16 12,15.2 9.3 19,22
Profile 5 16 15,20 12,13 17,18 X 11,12 29,33 15,16 13,15 7,9.3 25,26

Table 6. All male

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 2 15,16 15,19 - 18,20 XY 12,13 29 16,20 13,14 7,9.3 -
Profile 4 16 16,19 11,14 20 XY 10,13 28,29 13.2,20 14,15 7 23,25

Table 7. Potential match

Amelogen
D3S1358 vWA D16S539 D2S1338 D8S1179 D21S11 D18S51 D19S433 THO1 FGA
in
Profile 2 15,16 15,19 - 18,20 XY 12,13 29 16,20 13,14 7,9.3 -
Profile 5 15 15,20 12,13 17,18 X 11,12 29,33 15,16 13,15 7,9.3 25,26

Table 8. Genotype frequency of profile 1

Profile 1 p q 2pq p2 genotype frequency

D3S1358 14 11.25 0.1125 0.0500625 0.0500625
16 22.25 0.2225
vWA 16 19.75 0.1975 0.04345 0.04345
19 11 0.11
D16S539 10 4.25 0.0425 0.0014875 0.0014875
14 1.75 0.0175
D2S1338 20 13.5 0.135 0.029025 0.029025
23 10.75 0.1075
Amelogenin X

D8S1179 13 34.75 0.3475 0.12075625 0.12075625

D21S11 29 20.75 0.2075 0.0010375 0.0010375

33 0.25 0.0025
D18S51 16 14 0.14 0.0168 0.0168
18 6 0.06
D19S433 12 6.5 0.065 0.042575 0.042575
14 32.75 0.3275
THO1 6 25.25 0.2525 0.17675 0.17675
9.3 35 0.35
FGA 22 16.5 0.165 0.0462 0.0462
23 14 0.14
Student number: 12018448 Advanced DNA Examination 9th January 2018

Table 9. Genotype frequency of profile

- 2
Profile 3
D3S1358 15 28.25 0.2825 0.1257125 0.1257125
16 22.25 0.2225
vWA 18 25.75 0.2575 0.05665 0.05665
19 11 0.11
D16S539 10 4.25 0.0425 0.0252875 0.0252875
11 29.75 0.2975
D2S1338 19 12.75 0.1275 0.0095625 0.0095625
22 3.75 0.0375
Amelogenin X

D8S1179 13 34.75 0.3475 0.12075625 0.12075625

D21S11 28 16.25 0.1625 0.0674375 0.0674375

29 20.75 0.2075
D18S51 12 14.25 0.1425 0.0399 0.0399
16 14 0.14
D19S433 12 6.5 0.065 0.00325 0.00325
15.2 2.5 0.025
THO1 9.3 35 0.35 0.1225 0.1125

FGA 19 6.25 0.0625 0.020625 0.020625

Table 10. Genotype frequency of -profile 3

Profile 3
D3S1358 15 28.25 0.2825 0.1257125 0.1257125
16 22.25 0.2225
vWA 18 25.75 0.2575 0.05665 0.05665
19 11 0.11
D16S539 10 4.25 0.0425 0.0252875 0.0252875
11 29.75 0.2975
D2S1338 19 12.75 0.1275 0.0095625 0.0095625
22 3.75 0.0375
Amelogenin X

D8S1179 13 34.75 0.3475 0.12075625 0.12075625

D21S11 28 16.25 0.1625 0.0674375 0.0674375

29 20.75 0.2075
D18S51 12 14.25 0.1425 0.0399 0.0399
16 14 0.14
D19S433 12 6.5 0.065 0.00325 0.00325
15.2 2.5 0.025
THO1 9.3 35 0.35 0.1225 0.1125

FGA 19 6.25 0.0625 0.020625 0.020625

Student number: 12018448 Advanced DNA Examination 9th January 2018

Table 11. Genotype frequency of22profile 416.5 0.165

Profile 4
D3S1358 16 22.25 0.2225 0.04950625 0.04950625

vWA 16 19.75 0.1975 0.04345 0.04345

19 11 0.11
D16S539 11 29.75 0.2975 0.0104125 0.0104125
14 1.75 0.0175
D2S1338 20 13.5 0.135 0.018225 0.018225

Amelogenin XY

D8S1179 10 8 0.08 0.0556 0.0556

13 34.75 0.3475
D21S11 28 16.25 0.1625 0.0674375 0.0674375
29 20.75 0.2075
*D18S51 13.2 0.005 0.00005 0.0000015 0.0000015
20 1.5 0.015
D19S433 14 32.75 0.3275 0.1195375 0.1195375
15 18.25 0.1825
THO1 7 16 0.16 0.0256 0.0256

FGA 23 14 0.14 0.0315 0.0315

25 11.25 0.1125

Locus D18S51, allele 13.2 is not found in the population therefore had to use default value
of 0.005

Table 11. Genotype frequency of25profile11.25

5 0.1125
Profile 5
D3S1358 15 28.25 0.2825 0.07980625 0.07980625

vWA 15 8.25 0.0825 0.002475 0.002475

20 1.5 0.015
D16S539 12 34.25 0.3425 0.119875 0.119875
13 17.5 0.175
D2S1338 17 17.75 0.1775 0.0310625 0.0310625
18 8.75 0.0875
Amelogenin X

D8S1179 11 6.25 0.0625 0.0178125 0.0178125

12 14.25 0.1425
D21S11 29 20.75 0.2075 0.0010375 0.0010375
33 0.25 0.0025
D18S51 15 14.25 0.1425 0.0399 0.0399
16 14 0.14
D19S433 13 26 0.26 0.0949 0.0949
15 18.25 0.1825
THO1 7 16 0.16 0.112 0.112
9.3 35 0.35
FGA 25 11.25 0.1125 0.003375 0.003375
26 1.5 0.015