Jahe Merah

kons. Absrbansi sel perlakuan Rata2 Absorbansi Media Rata2

0.124 0.113
500 0.128 0.115 0.116
0.136 0.121
0.093
250 0.079
0.078
0.171
125 0.172
0.175
0.636
62.5 0.651
0.662
0.652
31.3 0.577
0.657
0.771
15.2 0.817
0.816
0.932
kontrol 0.913 0.915
0.899

yang dirata-rata adalah persen sel hidup

Conc % viability
500 1.671 coba dibuat grafik dari data ini
125 7.101
62.5 66.876 100.000
31.3 64.244 90.000
15.2 85.881 f(x) = - 0.6701244395x + 95.2277600759
80.000

70.000

60.000

50.000 Colu
Line
IC50 = 68 40.000
menulis IC50 adalah bulat, tidak ada koma 30.000

20.000

10.000

0.000
0 20 40 60 80 100 120 140
 Absorbansi sel-media % sel hidup rata2
0.008 1.003 1.671
0.012 1.504
0.020 2.506
-0.023 -2.882
-0.037 -4.637 dibuang
-0.038 -4.762
0.055 6.892 7.101
0.056 7.018
0.059 7.393
0.520 65.163 66.876
0.535 67.043
0.546 68.421
0.536 67.168 64.244
0.461 57.769
0.541 67.794
0.655 82.080 85.881
0.701 87.845
0.700 87.719

0.798

data 500 tidak dipakai karena pada kons 125 sel sudah mulai mati

(x) = - 0.6701244395x + 95.2277600759

Column B
Linear (Column B)

40 60 80 100 120 140

5Fluorourasil
kons. Absorbansi sel perlakuan Rata2 Absorbansi Media Rata2
0.683 0.113
1000 0.719 0.115 0.116
0.686 0.121
0.75
500 0.732
0.73
0.738
250 0.741
0.756
0.789
125 0.761
0.804
0.78
62.5 0.79
0.754
0.766
31.3 0.787
0.774
0.932
kontrol 0.913 0.915
0.899

86
1000 72.68
84
500 77.86
82 f(x) = - 0.0108122507x + 83.272859877
250 78.82 R² = 0.9232058131
125 83.79 80
62.5 82.54 78
31.3 82.66 76
74
IC50 = (50-83,27)/(-0.010)
3327 72
70
68
66
0 200 400 600 800 1000 1200
 Absorbansi sel-m% sel hidup rata2
0.567 71.053 72.68
0.603 75.564
0.57 71.429
0.634 79.449 77.86
0.616 77.193
0.614 76.942
0.622 77.945 78.82
0.625 78.321
0.64 80.201
0.673 84.336 83.79
0.645 80.827
0.688 86.216
0.664 83.208 82.54
0.674 84.461
0.638 79.950
0.65 81.454 82.66
0.671 84.085
0.658 82.456

0.798

0108122507x + 83.272859877
2058131

Column B
Linear (Column B)

400 600 800 1000 1200

5Fluorourasil
kons. Absorbansi seRata2 Absorbansi Medi Rata2 Absorbansi sel-me% sel hidup
0.89 0.087 0.796 84.411
1000 0.811 0.102 0.094 0.717 76.034
0.767 0.093 0.673 71.368
0.934 0.840 89.077
500 0.899 0.805 85.366
0.955 0.839 88.971
0.943 0.849 90.032
250 0.946 0.852 90.350
0.948 0.854 90.562
0.971 0.877 93.001
125 0.922 0.828 87.805
0.96 0.866 91.835
0.997 0.903 113.158
62.5 0.947 0.853 90.456
0.869 0.775 82.185
0.955 0.861 91.304
31.3 0.994 0.900 95.440
0.889 0.795 84.305
1.05
kontrol 1.033 1.037 0.943
1.028

conc %viability
1000 77.27 5fu
500 87.8 120
250 90.31
125 90.88 100
62.5 95.27 f(x) = - 0.0172576344x + 94.9933333333
80 R² = 0.9554231853
31.3 90.35
60
IC50 = (50-94,9)/(-0,017)
2641 40

20

0
0 200 400 600 800 1000
 rata2
77.27

87.80

90.31

90.88

95.27

90.35

5fu

44x + 94.9933333333
5fu
Linear (5fu)

600 800 1000 1200

5Fluorourasil
kons. Absorbansi sel Rata2 Absorbansi MedRata2 Absorbansi se % sel hidup rata2
0.869 0.087 0.775 82.185 78.61
1500 0.881 0.102 0.094 0.787 83.457
0.756 0.093 0.662 70.201
0.712 0.618 65.536 79.46
750 0.941 0.847 89.820
0.899 0.783 83.033
0.926 0.832 88.229 89.54
375 0.966 0.872 92.471
0.923 0.829 87.911
0.964 0.870 92.259 94.59
187.5 0.977 0.883 93.637
1.017 0.923 97.879
0.843 0.749 93.860 97.28
93.8 0.99 0.896 95.016
1.065 0.971 102.969
0.922 0.828 87.805 92.65
46.8 0.997 0.903 95.758
0.984 0.890 94.380
1.05
kontrol 1.033 1.037 0.943
1.028
conc %viability
1500 78.61
750 79.46 5fu
375 89.54
187.5 94.59 120
93.8 97.28
100
46.8 92.65 f(x) = - 0.0270715093x + 99.7351658883
80 R² = 0.9999213415
5fu
IC50 = (50-99,73)/-0,027 Linear (5fu)
1842 60

40

20

0
0 100 200 300 400 500 600 700 800
 5fu
Linear (5fu)

800
5Fluorourasil
kons. AbsorbansiRata2 AbsorbansRata2 Absorbansi% sel hidu rata2
0.156 0.087 0.062 6.575 6.89
30000 0.155 0.102 0.094 0.061 6.469
0.166 0.093 0.072 7.635
0.666 0.572 60.657 56.38
15000 0.61 0.516 54.719
0.623 0.507 53.765
0.688 0.594 62.990 67.16
7500 0.745 0.651 69.035
0.749 0.655 69.459
0.685 0.591 62.672 69.85
3750 0.825 0.731 77.519
0.748 0.654 69.353
0.75 0.656 82.206 81.13
1875 0.832 0.738 78.261
0.876 0.782 82.927
0.794 0.700 74.231 80.38
937.5 0.854 0.760 80.594
0.908 0.814 86.320
1.05
kontrol 1.033 1.037 0.943
1.028
0.803 0.803 85.154 95.72
468.75 0.951 0.951 100.848
0.954 0.954 101.166

conc %viability
120
30000 6.89
15000 56.38
100
7500 67.16
3750 69.85
80 f(x) = - 0.0025957013x + 87.4336206897
1875 81.13 R² = 0.9501395307
937.5 80.38
60 Column B
468.75 95.72
Linear (Column B)
40

20

0
0 5000 10000 15000 20000 25000 30000 35000
Column B
Linear (Column B)
5Fluorourasil
kons. Absorbansi sRata2 Absorbansi Media Rata2 Absorbansi sel- % sel hidup rata2
0.137 0.087 0.043 4.560 4.95
40000 0.144 0.102 0.094 0.050 5.302
0.141 0.093 0.047 4.984
0.299 0.205 21.739 20.54
20000 0.293 0.199 21.103
0.293 0.177 18.770
0.616 0.522 55.355 58.71
10000 0.71 0.616 65.323
0.617 0.523 55.461
0.587 0.493 52.280 58.75
5000 0.667 0.573 60.764
0.69 0.596 63.203
0.759 0.665 83.333 73.87
2500 0.704 0.610 64.687
0.788 0.694 73.595
0.684 0.590 62.566 64.93
1250 0.642 0.548 58.112
0.793 0.699 74.125
1.05
kontrol 1.033 1.037 0.943
80
1.028
0.745 70
0.745 f(x) = - 0.0028380174x
79.003 81.30 + 79.5739130435
R² = 0.9307941448
625 0.79 0.790
60 83.775
0.765 0.765 81.124
50
conc %viability 40
40000 4.95
20000 20.54 30
10000 58.71 20
5000 58.75
2500 73.87 10
1250 64.93
0
625 81.3 0 5000 10000 15000 20000 250

IC50 = (50-79,57)/-0,002
14785
.0028380174x + 79.5739130435
07941448

Column B
Linear (Column B)

10000 15000 20000 25000