MOLECULAR
GENETICS
BIO20 Introduction
to Biomimetics Prof. Ureah Thea A. Sevilla
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Deoxynucleoside triphosphates
Deoxyadenosine Deoxycytidine triphosphate,
triphosphate, dATP dCTP
Deoxyguanosine Deoxythymidine
triphosphate, dGTP triphosphate, dTTP
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5’ 3’ Polynucleotide Chain
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Anti-parallel strands of
the DNA
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3.4-nanometer length of
each full twist of the double
helix (360 ° turn)
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Costed $3 billion
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DNA Replication
DNA replication is an essential aspect of cellular
and viral reproduction.
Replication of a double-stranded DNA results in two
double-stranded DNAs as products.
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DNA Replication
DNA replication is an
exponential process.
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DNA helicase
Breaks hydrogen bonds between DNA strands
DNA polymerase
Joins free nucleotides into a new strand of DNA
DNA ligase
Joins DNA segments on discontinuous strand
INITIATION STEP
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ELONGATION STEP
Elongation Step
At a replication fork, one daughter strand (the
leading strand) is elongated continuously. The other
daughter strand (the lagging strand) is formed as a
series of discontinuous.
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TERMINATION STEP
DNA ligase
JoinsDNA segments on
discontinuous strand
Leading strand
– continuous synthesis
Lagging strand
– discontinuous synthesis
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Problems
What is the complimentary sequence of the given
DNA strand?
Problems
What is the complimentary sequence of the given
DNA strand?
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Structures of RNA
Ribosomal RNA, rRNA
isassociated with a number of different proteins as
components of the ribosome (complex as site for
protein synthesis)
80% of the RNA in the cell
Structures of RNA
Messenger RNA, mRNA
carries genetic information from the DNA to the
cytosol, where it is used as the template for protein
synthesis
5% of the RNA in the cell
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Structures of RNA
rRNA
tRNA
POST-TRANSCRIPTIONAL
MODIFICATION (Eukaryotes)
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Base-Pairing in
DNA Synthesis and Transcription
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Problems
Given the DNA template, what is the sequence of
the RNA produced?
Problems
Given the DNA template, what is the sequence of
the RNA produced?
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Termination of Transcription
Rho-independent
termination
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Termination of Transcription
Rho-dependent Termination
The Rho factor acts as an ATP-dependent unwinding enzyme,
moving along the newly forming RNA molecule towards its 3′
end and unwinding it from the DNA template as it proceeds.
Post-Transcriptional Modifications
In eukaryotes, RNA is modified before it leaves the
nucleus as a mature mRNA
Introns (noncoding sequence)
Nucleotide sequences that are removed from a new
RNA
Exons (coding sequence)
Sequences that stay in the RNA
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Alternative Splicing
Alternative splicing
Allows one gene to encode different proteins
Introns are removed from RNA and exons are spliced
together in various combinations
Addition of modified GTP at the 5’-end and
addition of poly-Adenines at 3’-end
Aftersplicing, transcripts are finished with a modified
guanine “cap” at the 5' end and a poly-A tail at the 3'
end.
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POST-TRANSLATIONAL
MODIFICATION (eukaryotes)
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Problems
Given the DNA template, what is the amino acid
sequence when it is translated?
Problems
Given the DNA template, what is the amino acid
sequence when it is translated?
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The Ribosome
Ribosome of E. coli
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References:
Voet, Voet and Pratt, Principles of Biochemistry 3rd
ed. Wiley Publication (2008).
Lodish H, Berk A, Zipursky SL, et al. Molecular Cell
Biology, 5th edition. New York: W. H. Freeman;
(2004).
Barnum, S., Biotechnology, Wadsworth – Thomson
Publishing Co. (1998).
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