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Many plant tissues change colour when exposed to the air. The enzymes that produce the
coloured products may be affected by pH.
Investigation
You will investigate the effect of pH on the production of these coloured products formed on
the freshly cut surface of a plant tissue.
(a) You are provided with 2 pieces of plant tissue, labelled W1, that have been cut before
the start of the examination.
(i) Use one piece of W1 to compare the colour of the exposed cut surface with the
unexposed surface which is in contact with the dish.
Keep this piece of W1 in the dish for part (b).
(ii) You have been provided with 2 pieces of universal indicator paper and access to a
pH colour chart.
Test the pH of solutions A1 and B1.
Record the colours and pH values in Table 1.1.
Table 1.1
solution
A1 B1
colour
pH
[3]
[3]
(iv) Describe and explain the effect of pH on the development of the coloured products
in this plant tissue.
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...............................................................................................................................[2]
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Table 1.2
W1
test
observation conclusion
iodine
solution
biuret
[4]
(iii) Describe briefly how you would carry out a test for simple reducing sugars. State
what observation would indicate the presence of a reducing sugar.
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...............................................................................................................................[3]
[Total : 21]
(a) (i) Make a large labelled outline drawing of the whole leaf and show the details of one
pair of leaflets. Include at least three labels.
[9]
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...............................................................................................................................[2]
Fig. 2.1
(i) Name and label on Fig. 2.1, the different layers of cells. Identify clearly those cells
which contain chloroplasts. [4]
(ii) Name and label a feature on Fig. 2.1 that enables gaseous exchange to occur. [1]
(iii) The section of the leaf is magnified by × 200. Calculate the thickness of the leaf.
working
[Total : 19]
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University of Cambridge International Examinations is part of the University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of
the University of Cambridge.
0610/05/O/N/04