SULIT 2 4551/3

Answer all questions

Jawab semua soalan

1. A group of student carried out an experiment to study the effect of temperature on

the rate of amylase reaction on starch.
Sekumpulan pelajar menjalankan eksperimen untuk mengkaji kesan suhu terhadap
kadar tindakbalas amilase terhadap kanji.

Diagram 1 shows the apparatus set-up of the experiment.

Rajah 1 menunjukkan susunan radas untuk eksperimen tersebut.

Step 1 : 1ml of saliva solution and 5 ml of 1% starch suspension were placed in

two different test tube in a water bath at 5° C for 5 minute.
Langkah 1 : 1ml larutan air liur dan 5 ml 1% ampaian kanji diletakkan ke dalam dua
tabung uji yang berbeza di dalam kukus air 5° C selama 5 minit.

Step 2 : After 5 minute the saliva solution was added to the starch suspension.
Langkah 2 : Selepas 5 minit larutan air liur itu dimasukkan ke dalam ampaian kanji
tersebut.

Step 3 : At one minute intervals two drop of the mixture was withdrawn with
a dropper and placed on a drop of iodine solution on a white tile.
Langkah 3 : Dalam selang masa satu minit, dua titis campuran itu di ambil
menggunakan penitis dan dititiskan pada satu titik larutan iodin di atas
jubin putih.

Step 4 : Step 3 was repeated until the blue black solution turn into yellowish.
The time taken is recorded.
Langkah 4 : Langkah 3 diulang sehingga warna larutan biru gelap bertukar
kekuningan. Masa yang diambil dicatatkan.

Step 5 : Step 1 to 5 is repeated using water baths at 5° C, 28° C,37° C , 45° C

and 55° C.
Langkah 5 : Langkah 1 hingga langkah 4 di ulang dengan menggunakan suhu
kukus 5° C, 28° C,37° C , 45° C dan 55° C.

water
air
ice
ais

5 ml of 1% starch suspension 1 ml of saliva solution

5 ml 1% ampaian kanji 1 ml larutan air liur

Diagram 1
Rajah 1

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SULIT 3 4551/3

(a) Record the time taken for the iodine solution remains yellowish in the boxes
provided in Table 1.
Rekodkan masa yang di ambil untuk warna iodine kekal kekuningan di dalam
kotak yang disediakan dalam Jadual 1. [ 3 marks]
[3 markah]
Temperature Time taken for iodine solution remains yellowish / (minute)
Suhu (° C) Masa yang diambil untuk warna iodine kekal kekuningan/ (minit)

28

37

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 SULIT 4 4551/3

45

55

For
Examiner’s Table 1
Use Jadual 1

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1(a)
 SULIT 5 4551/3

For
(b) (i) State two different observations made from Table 1. Examiner’s
Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 1. Use

Observation 1 /
Pemerhatian 1 :

__________________________________________________________

__________________________________________________________

__________________________________________________________

Observation 2 /
Pemerhatian 2 :

__________________________________________________________
1(b)(i)
__________________________________________________________

__________________________________________________________
[ 3 marks]
[3 markah]

(ii) State the inference which corresponds to the observations in 1 (b) (i).
Nyatakan inferens yang sepadan dengan pemerhatian di 1 (b) (i).

Inference from observation 1 /

Inferens daripada pemerhatian 1 :

__________________________________________________________

__________________________________________________________

__________________________________________________________

Inference from observation 2 /

Inferens daripada pemerhatian 2 :

__________________________________________________________
1(b)(ii)
__________________________________________________________

__________________________________________________________
[ 3 marks]
[3 markah]

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 For SULIT 6 4551/3
Examiner’s
Use

(c) Complete Table 2 based on this experiment

Lengkapkan Jadual 2 berdasarkan eksprimen ini

Variable Method to handle the variable

Pembolehubah Cara mengendali pembolehubah
Manipulated variable
Pembolehubah dimanipulasi

________________________ ________________________

________________________ ________________________

________________________ ________________________

Responding variable
Pembolehubah bergerakbalas

________________________ ________________________

________________________ ________________________

________________________ ________________________

Constant variable
Pembolehubah dimalarkan

________________________ ________________________

________________________ ________________________

________________________ ________________________
1(c)
Table 2
Jadual 2
[ 3 marks]
[3 markah]

(d) State the hypothesis for this experiment.

Nyatakan hipotesis bagi eksperimen ini.

_______________________________________________________________

_______________________________________________________________
1(d)
_______________________________________________________________

[ 3 marks]
[3 markah]

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SULIT 7 4551/3 For
Examiner’s
Use

(e) (i) Construct a table and record all the data collected in this experiment.
Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimen
ini.

Your table should have the following title.

Jadual anda hendaklah mengandungi tajuk-tajuk berikut.`

 Temperature
Suhu

 Time taken for iodine solution remains yellowish

Masa yang di ambil untuk warna iodin kekal kekuningan

 Rate of enzyme reaction

Kadar tindakbalas enzim

1(e)(i)

[ 3 marks]
[3 markah]

(ii) Use the graph paper provided on page 8 to answer this part of
question. Using the data in 1(e)(i), draw the graph of the rate of
enzyme reaction against the temperature.
Gunakan kertas graf yang disediakan dalam di mukasurat 8 untuk
menjawab soalan bahagian ini. Menggunakan data 1(e)(i), lukiskan
graf kadar tindakbalas enzim melawan suhu.
1(e)(ii)
[ 3 marks]
[3 markah]

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SULIT 8 4551/3

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 SULIT 9 4551/3 For
Examiner’s
Use
(f) Based on the graph in 1 (e) (ii) , explain the relationship between rate of enzyme
reaction and temperature .
Berdasarkan kepada graf di 1 (e) (ii), terangkan hubungan antara kadar
tindakbalas enzim dengan suhu.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________ 1(f)

[ 3 marks]
[3 markah]

(g) This experiment is repeated by increasing the concentration of the saliva

solution. Predict the time taken for iodine solution remains yellowish.
Explain your prediction.
Eksperimen ini diulang dengan menambah kepekatan larutan air liur. Ramalkan
masa yang di ambil untuk warna iodine kekal kekuningan.
Terangkan ramalan anda.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________ 1(g)

[ 3 marks]
[3 markah]

(h) Based on the result from the experiment what can be deduced about enzyme.
Berdasarkan keputusan daripada eksperimen ini, apakah yang dapat dirumuskan
tentang enzim.

_______________________________________________________________

_______________________________________________________________
1(h)
_______________________________________________________________

[ 3 marks]
[3 markah]

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 SULIT 10 4551/3

For
Examiner’s
Use
(i) The following is a list of enzyme and medium for reaction.
Berikut ialah senarai enzim dan medium untuk bertindakbalas.

Alkaline Trypsin Amylase

Beralkali Tripsin Amilase

Pepsin Neutral Acidic

Pepsin Neutral Berasid

Classify the enzyme with the suitable medium for reaction in Table 3.
Klasifikasikan enzim tersebut dengan medium tindakbalas yang sesuai di
dalam Jadual 3.

Enzyme Medium for reaction

Enzim Medium untuk bertindakbalas

Table 3
Jadual 3

1(i)

[ 3 marks]
[3 markah]

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 SULIT 11 4551/3

2. A group of students carry out an experiment to study the effect of environmental

factor on the rate of transpiration of a balsam plant.
Sekumpulan pelajar menjalankan eksperimen untuk mengkaji kesan faktor
persekitaran terhadap kadar transpirasi pokok keembong.

Based on the above information, plan a laboratory experiment to determine the

effect of the temperature on the rate of transpiration
Berdasarkan maklumat di atas , rancang experiment dalam makmal untuk
mengkaji kesan suhu terhadap kadar transpirasi

The planning of your experiment must include the following aspects:

Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:

. The planning of your experiment must include the following aspects:

Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:

 Problem statement
Penyataan masalah

 Objective of investigation
Objektif kajian

 Variables
Pembolehubah-pembolehubah

 Hypothesis
Hipotesis

 List of materials and apparatus

Senarai bahan dan radas digunakan

 Technique used
Teknik yang digunakan

 Experimental procedure or method

Kaedah atau prosedur eksperimen

 Presentation of data
Cara data dipersembahkan

 Conclusion
Kesimpulan [17 marks]
[17 markah]
END OF QUESTION PAPER
KERTAS SOALAN TAMAT

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