Milestones Group #10

Milestone #1: Dominic Ventre

Bio-glue Test on Rats Protocol

Purpose: To test the effectiveness of our Bio-Band Aid Product. Which consists of super glue like
material designed to cover a wound in order to reinforce defense against adhesion.

Hypothesis: The control group rat will have the slowest healing process due to environmental and rats
self-interruption. The rat with the Bio-Band Aid will have the fastest healing speed due to the enhanced
formula.

Materials: Bio-Band Aid(product), unmodified glue, gauss, three rats, plain bio-glue, scalpel, Rat cage,
sedative drug for rats(if necessary), sharpie marker, ruler, small spatula, razor blade.

Procedure:

1) Receive three rats of equal health and same gender. Drug them with the sedative (if necessary).

2) Remove any fur in place of incision.

3) Using the scalpel, make an inch long cut (does not have to be deep) on the chest of the rat within
reach of the rat’s arm to scratch. If no sedative is applied, have someone hold the rat down so the
incision process is not interrupted.

4) This rat will be the control group. Use a sharpie to draw the number 1 on this rat.

5) Hold gauss on the rats cut until bleeding stops. When bleeding stops, place the rat in the cage.

6) Repeat step three for the next rat. It is very important that the cuts on each rat are the same size!

7) Get the unmodified glue ready to apply to the rats wound by dabbing a few drops on the small
spatula (enough to cover an inch long cut)

8) Apply the glue by first using the gauss to wipe any blood off of the wound, then carefully spreading
the glue over the wound so that it covers the whole cut. Try to restrict the glue to the cut as much as
possible.

9) Height of the glue should be a fourth of a centimeter or less.

10) Glue surface should be smooth to prevent snags.

11) Steps 7-10 must be performed quickly for the glue dries fast.

12) Draw a number 2 on this rat. Hold rat until glue dries. Be sure to prevent the rat from scratching.
Place in cage when glue is dried.

13) For rat number 3, using Bio-band aid product, repeat steps 6-11. The Bio-Band Aid will have the
same general properties of the unmodified glue and therefore be applied in the same way.

14) Repeat step 12 except draw a number 3 on this rat.

15) Observational data will be collected once a week noting the healing progress.
Clean up:

- Use gauss to wipe up any blood from the rats before it dries. Use rubbing alcohol to sterilize the areas
where spill occurred.

- Use a razor blade to scrap off any spilled glue and dispose of properly.

- Make sure gauss is disposed of properly as well.

Milestone #2: Dominic Ventre

Bio-glue Test on Rats Protocol

I accompanied Dr. Prashanthi on her visit to the lab where rat experimentation occurs. We spoke with
the head of the facility who said that it is possible for us to hold the Bio-glue experiment in a separate
lab if we can get the protocol approved by the end of quarter. As soon as the protocol is approved I am
to be registered for animal testing. We then present the approved project the head of the rat testing
facility once again who will then approve the experiment to be done in a lab.

Milestone #3: Jason Tran

Abrasion Test Design

The abrasion test is designed to test the durability of both the scabs and our Bio-Bandaid. The abrasion
test part that moves will move up and down a set of 4 metal rods and the abrasion tester will move
forwards and backwards. Both the top and bottom will contain pieces of leather that will rub against
each other. Below is the final design. The operation of this test can be explained better in person.

Top:
 Bottom:

Final Design:
Milestone #4: Jason Tran/John Tamase

Machining (Jason Tran)

In the machining workshops located in the undergraduate ThinkTank, my team and I will test our various
bandaid materials with leather and will also use the shop to construct our abrasion tester. With Doug
and his assistant, we will use all the materials to construct the products needed for our project safely.
We will learn how to operate various machines from drill presses to jigsaws.

Comsol (John Tamase)

For milestone 4 we plan to learn the basics of COMSOL and apply it to our project. our objectives include
creating models and use it to gain a Better understanding of our project. Our models will be projected
towards The permeability of skin , blood, antibiotics, and super glue and maybe other adhesives. The
coefficient of skin and glue will be used in order to create the model and simulate the permeability. The
calculation for the permeability coefficient can be incorporated using Darcy's Law. It can be used with
the combination of fluid material and a porous material which can be imputed into COMSOL. The use of
this software can provide a basic visual image for our biobandaid project.

Milestone #5: Aaron Tan

Materials

The majority of required materials for our group project pertain to components for assembly of our
bandage and our test mechanism. Our product The abrasive system will be composed from plywood,
rubber, leather, and metal rods. These materials will be obtained from the home improvements store -
Home depot. Tools needed to put our machine together include a jig-saw, drill press, and wood glue will
be available for use through the Think Tank at UCR. Pig's blood, which will be used in substitute for
human blood, will also be used in conjunction with our abrasive mechanism to test the durability and
efficiency of our product. This will be obtained from a butcher shop. For actual lab trials, it has been
proposed that 4 lab rats will be used. These will be provided by the lab, should permission be granted.