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TOOLS OF BIOCHEMISTRY

Laboratory work No. 2.

EQUIPMENT’S USED IN A BIOCHEMISTRY LABORATORY

Introduction

The aims of biochemical and molecular biological research are complex and diverse. Investigation of the
network of chemical reactions taking place in living organisms and representing the most fundamental
phenomena of life, identification of the molecules playing roles in biochemical processes, determination of
their structure, function and interactions, examination of the molecular background of metabolism, the flow
of energy and information within organisms are all among the common goals of biochemists and molecular
biologists. In accordance with this diversity of problems, a high variety of tools, instruments and methods are
required to answer scientific questions effectively. This practical reviews the most common tools and
instruments used very frequently in almost every laboratory. The appropriate handling and uses set of
equipment required for biochemistry or molecular biology research will also be discussed.

Aim: To demonstrate the following to the students

1. Various laboratory equipment for Biochemistry work


2. Their basic functions and handling

Activity Materials Required Remarks


Different laboratory equipment  Display of available Demonstration and explanations
used in a Biochemistry equipment. especially with regards to
laboratory function and how used.
 Refer to the handout

Conclusions:

What are the principles behind Chromatography, electrophoresis, photometry (colorimeter &
spectrophotometer), centrifugation?