DAFTAR ISI

JUDUL ................................................................................................................. i
HALAMAN PENGESAHAN ............................................................................. ii
KATA PENGANTAR ......................................................................................... iii
DAFTAR ISI ........................................................................................................ v
DAFTAR TABEL ............................................................................................... vii
DAFTAR GAMBAR ........................................................................................... viii
BAB I PENDAHULUAN
1.1 Latar Belakang ................................................................................. 1
1.2 Rumusan Masalah ............................................................................ 7
1.3 Tujuan Penelitian ............................................................................. 7
1.4 Manfaat Penelitian ........................................................................... 8
1.5 Batasan Masalah .............................................................................. 8

BAB II TINJAUAN PUSTAKA

2.1 Mikroalga Chlorella sp. .................................................................. 9
2.2 Kultivasi Mikroalga Chlorella sp. .................................................. 10
2.3 Ekstraksi Senyawa Aktif .................................................................. 14
2.4 Hidrolisis dan Partisi. ....................................................................... 17
2.5 Senyawa Steroid .............................................................................. 18
2.6 LCMS (Liquid Chromatography Mass Spectrum) .......................... 20
2.7 Pemisahan Senyawa Aktif dengan Kromatografi Lapis Tipis ......... 21
2.8 Uji Toksisitas Senyawa Aktif Larva Udang Artemia salina Leach . 24
2.8.1. Morfologi Larva Udang Artemia salina Leach ...................... 24
2.8.2. Uji Toksisitas terhadap Larva Udang Artemia salina Leach
dengan BSLT .......................................................................... 26
2.9 Analisis Probit.................................................................................. 28

BAB III METODOLOGI PENELITIAN

3.1 Pelaksanaan Penelitian ..................................................................... 30
3.2 Alat dan Bahan................................................................................. 30
3.2.1 Alat ......................................................................................... 30
3.2.2 Bahan ...................................................................................... 30
3.3 Rancangan Penelitian ....................................................................... 31
3.4 Tahapan Penelitian ........................................................................... 32
3.5 Pelaksanaan Penelitian ..................................................................... 33
3.5.1 Kultivasi Mikroalga Chlorella sp. .................................... 33
3.5.1.1 Pembuatan Medium Ekstrak Tauge (MET) 4% ......... 33
3.5.1.2 Kultivasi Mikroalga Chlorella sp. dalam Medium
Ekstrak Tauge (MET) 4%........................................... 33
3.5.1.3 Pemanenan Biomassa Mikroalga Chlorella sp. ......... 33
3.5.2 Preparasi Sampel Biomassa Mikroalga Chlorella sp. ........... 34
3.5.3 Analisis Kadar Air Biomassa Mikroalga Chlorella sp. ......... 34
3.5.4 Ekstraksi Komponen Aktif ..................................................... 34
3.5.5 Hidrolisis Ekstrak Metanol Mikroalga Chlorella sp. ............. 35

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 3.5.6 Partisi Ekstrak Metanol Mikroalga Chlorella sp. .................. 35
3.5.7 Identifikasi Menggunakan LC-MS ......................................... 36
3.5.8 Pemisahan Senyawa Steroid Menggunakan Kromatografi
Lapis Tipis .............................................................................. 36
3.5.9 Uji Toksisitas Biomassa Mikroalga Chlorella sp. terhadap
Larva Udang Artemia salina Leach........................................ 37
3.4.9.1 Penetesan Larva Udang Artemia salina Leach........... 37
3.4.9.2 Uji Toksisitas .............................................................. 37
3.6 Analisa Data ..................................................................................... 38

DAFTAR PUSTAKA .......................................................................................... 39

LAMPIRAN ......................................................................................................... 45

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